MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Oxygen-dependent binding of Nro1 to the prolyl hydroxylase Ofd1 regulates SREBP degradation in yeast. Oxygen-dependent binding of Nro1 to the prolyl hydroxylase Ofd1 regulates SREBP degradation in yeast. EMBO J. (0261-4189) 2009 imex curation Lee CY., Stewart EV., Hughes BT., Espenshade PJ. 2009-03-09 Only protein-protein interactions schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Lee CY., Stewart EV., Hughes BT., Espenshade PJ. EMBO J. (0261-4189) 2009 2009-03-09 Only protein-protein interactions imex curation Oxygen-dependent binding of Nro1 to the prolyl hydroxylase Ofd1 regulates SREBP degradation in yeast. Oxygen-dependent binding of Nro1 to the prolyl hydroxylase Ofd1 regulates SREBP degradation in yeast. EMBO J. (0261-4189) 2009 imex curation Lee CY., Stewart EV., Hughes BT., Espenshade PJ. 2009-03-09 Only protein-protein interactions schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lee CY., Stewart EV., Hughes BT., Espenshade PJ. EMBO J. (0261-4189) 2009 2009-03-09 Only protein-protein interactions imex curation Oxygen-dependent binding of Nro1 to the prolyl hydroxylase Ofd1 regulates SREBP degradation in yeast. Oxygen-dependent binding of Nro1 to the prolyl hydroxylase Ofd1 regulates SREBP degradation in yeast. EMBO J. (0261-4189) 2009 imex curation Lee CY., Stewart EV., Hughes BT., Espenshade PJ. 2009-03-09 Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Lee CY., Stewart EV., Hughes BT., Espenshade PJ. EMBO J. (0261-4189) 2009 2009-03-09 Only protein-protein interactions imex curation ett1_schpo Negative regulator of ofd1 SPCC4B3.07 nro1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MIGRRPQGLRAAASLKKQQQLEKQKQEASYELSGNSSPSKENGSENVDNGEMEDETMLVYTEEDNISQLWGLYEMSREKLENDDIDASVSLVFGTIHEADRILRNTEDISTLPKDFHAAYSSALLAVSELFEIAQKRLKETNTEESYIDAAIERAQLGLDAPGNESRLFLALARAYLEKVRVLVWRHDNEESLANIPVTQLVNPYIEKAIQYLRPLAQDSTEYFDALTPDSLRPLYILSSYLFQFGDQFSEAFLLDVCSIITALWLKSVVDPNTPAYYKLIAQEAVLNNYTTFAEYYMDLLDNSESNVDDLINKASSWLNNSVDTWNVIYTLDKSPERLLKLADIKMDLAQIVQDEASQDNYLKEACNAIKEAQGSGVELSPDYVEFVEAYSA 081D5A027088DDDC mint 081D5A027088DDDC ofd1_schpo Prolyl 3,4-dihydroxylase ofd1 PKHD-type hydroxylase ofd1 ofd1 2-oxoglutarate and Fe(II) dioxygenase domain-containing protein 1 uS12 prolyl 3,4-dihydroxylase SPBC6B1.08c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MGDTQQPTVLDRFAPHALDTSEAERLSREYHKNGPYNHVVIRPLINDTLLRNVRKELMENIHFTEKVTDIYRVWQTGDLANLDGLDKKEQNMLKYLRQVRDALYSEEFRSHVQKITGCGPLSASKKDLSVNVYSKGCHLMNHDDVIGTRCISYILYLVEPDEGWKPEYGGALRLFPTLQPSFPAADFCHSIPPQWNQLSFFRVKPGHSFHDVEEVYVDKPRMAISGWFHYPQPGEPGYDANYCDNTVSTLQSLTMKQMDLELPRFSYYPVVKPEPLSKQDTDILSNYINPLYLTPDGIEKLSKRFFQDSVIVLVEFLNQEFANTLLKRIIDAERQPTPMHSSEVSFPWKTAIPPHKHRYLYLDHEEFGPDIILPMDLQRLPAFQRWIQLVSGLPLRSFHQVGRRFRPGSDFTLATTNDTALLEATLCLSPGTGIANTDNGAYDIYMIGDEDPDVDAAVYRGDQQDDSILLSLPAAWNVFSLVYRDEGVLQFVKYVSRQAESSRWDIYSQWNPVAE 4A6EBD870424EE0B mint 4A6EBD870424EE0B ofd1-nro1 2 ofd1 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Nro1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association F5B F7B F7C mint nro1-ofd1 2 Nro1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level ofd1 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region certain certain sequence position certain c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal range MINT-7011898: original sequence: 242-515 : YCDNTVSTLQSLTMKQMDLELPRFSYYPVVKPEPLSKQDTDILSNYINPLYLTPDGIEKLSKRFFQDSVIVLVEFLNQEFANTLLKRIIDAERQPTPMHSSEVSFPWKTAIPPHKHRYLYLDHEEFGPDIILPMDLQRLPAFQRWIQLVSGLPLRSFHQVGRRFRPGSDFTLATTNDTALLEATLCLSPGTGIANTDNGAYDIYMIGDEDPDVDAAVYRGDQQDDSILLSLPAAWNVFSLVYRDEGVLQFVKYVSRQAESSRWDIYSQWNPVA physical association physical association F5D mint ofd1-nro1-1 2 ofd1 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Q11120:p.His210Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain H A Tue Jul 12 10:51:42 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 17:04:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his210ala' Tue Jun 30 18:08:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His210Ala' range MINT-7011916: original sequence: 210-210 : FFRVKPGHSFHDVEEVYVDK Nro1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association F6B mint ofd1-nro1-2 2 ofd1 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Q11120:p.His142Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain H A Tue Jul 12 10:10:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 14:40:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his142ala' Tue Jun 30 20:04:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His142Ala' range MINT-7011922: original sequence: 142-142 : VYSKGCHLMNHDDVIGTRCI Nro1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association F6B mint ofd1-nro1-3 2 ofd1 5 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Q11120:p.Asp144Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A Tue Jul 12 10:51:54 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:50:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp144ala' Tue Jun 30 17:16:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp144Ala' range MINT-7011940: original sequence: 144-144 : SKGCHLMNHDDVIGTRCISY Q11120:p.His142Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain H A Tue Jul 12 10:11:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:01:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his142ala' Tue Jun 30 16:08:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His142Ala' range MINT-7011935: original sequence: 142-142 : VYSKGCHLMNHDDVIGTRCI Nro1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association F6B mint nro1-ofd1-1 1 Nro1 4 anti tag immunost anti tag immunostaining anti tag immunost predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell myc tagged myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ofd1 5 immunostaining immunostaining predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization F5A mint ofd1-nro1-4 3 Nro1 4 western blot western blot Immuno blot Immuno blot predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ofd1 5 anti tag western anti tag western blot anti tag western predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction F5C mint