IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. Biochem. Biophys. Res. Commun. (0006-291X) 2008 imex curation Nonaka H., Takei K., Umikawa M., Oshiro M., Kuninaka K., Bayarjargal M., Asato T., Yamashiro Y., Uechi Y., Endo S., Suzuki T., Kariya K. kariya@med.u-ryukyu.ac.jp 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions in vitro In vitro pull down pull down de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Nonaka H., Takei K., Umikawa M., Oshiro M., Kuninaka K., Bayarjargal M., Asato T., Yamashiro Y., Uechi Y., Endo S., Suzuki T., Kariya K. kariya@med.u-ryukyu.ac.jp Accepted 2009-FEB-28 by DAVET. 09-April-2009: Contacted by JYOTI. Biochem. Biophys. Res. Commun. (0006-291X) 2008 davet Only protein-protein interactions imex curation MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. Biochem. Biophys. Res. Commun. (0006-291X) 2008 imex curation Nonaka H., Takei K., Umikawa M., Oshiro M., Kuninaka K., Bayarjargal M., Asato T., Yamashiro Y., Uechi Y., Endo S., Suzuki T., Kariya K. kariya@med.u-ryukyu.ac.jp 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nonaka H., Takei K., Umikawa M., Oshiro M., Kuninaka K., Bayarjargal M., Asato T., Yamashiro Y., Uechi Y., Endo S., Suzuki T., Kariya K. kariya@med.u-ryukyu.ac.jp Accepted 2009-FEB-28 by DAVET. 09-April-2009: Contacted by JYOTI. Biochem. Biophys. Res. Commun. (0006-291X) 2008 davet Only protein-protein interactions imex curation MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. Biochem. Biophys. Res. Commun. (0006-291X) 2008 imex curation Nonaka H., Takei K., Umikawa M., Oshiro M., Kuninaka K., Bayarjargal M., Asato T., Yamashiro Y., Uechi Y., Endo S., Suzuki T., Kariya K. kariya@med.u-ryukyu.ac.jp 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Nonaka H., Takei K., Umikawa M., Oshiro M., Kuninaka K., Bayarjargal M., Asato T., Yamashiro Y., Uechi Y., Endo S., Suzuki T., Kariya K. kariya@med.u-ryukyu.ac.jp Accepted 2009-FEB-28 by DAVET. 09-April-2009: Contacted by JYOTI. Human foetal brain cDNA Biochem. Biophys. Res. Commun. (0006-291X) 2008 davet Only protein-protein interactions imex curation MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. Biochem. Biophys. Res. Commun. (0006-291X) 2008 imex curation Nonaka H., Takei K., Umikawa M., Oshiro M., Kuninaka K., Bayarjargal M., Asato T., Yamashiro Y., Uechi Y., Endo S., Suzuki T., Kariya K. kariya@med.u-ryukyu.ac.jp 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Nonaka H., Takei K., Umikawa M., Oshiro M., Kuninaka K., Bayarjargal M., Asato T., Yamashiro Y., Uechi Y., Endo S., Suzuki T., Kariya K. kariya@med.u-ryukyu.ac.jp Accepted 2009-FEB-28 by DAVET. 09-April-2009: Contacted by JYOTI. Biochem. Biophys. Res. Commun. (0006-291X) 2008 davet Only protein-protein interactions imex curation MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1. Biochem. Biophys. Res. Commun. (0006-291X) 2008 imex curation Nonaka H., Takei K., Umikawa M., Oshiro M., Kuninaka K., Bayarjargal M., Asato T., Yamashiro Y., Uechi Y., Endo S., Suzuki T., Kariya K. kariya@med.u-ryukyu.ac.jp 09-April-2009: Contacted by JYOTI. davet Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Nonaka H., Takei K., Umikawa M., Oshiro M., Kuninaka K., Bayarjargal M., Asato T., Yamashiro Y., Uechi Y., Endo S., Suzuki T., Kariya K. kariya@med.u-ryukyu.ac.jp Accepted 2009-FEB-28 by DAVET. 09-April-2009: Contacted by JYOTI. Biochem. Biophys. Res. Commun. (0006-291X) 2008 davet Only protein-protein interactions imex curation q5eb61_rat LOC303259 protein protein rat Rattus norvegicus (Rat) Rat EGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIVFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW C1BF27CC1657BF93 tnik_human TRAF2 and NCK-interacting protein kinase TNIK KIAA0551 protein protein human Homo sapiens Human MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRPVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVTSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPILESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPAKVKPEESRDITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPMKKVTDYSSSSEESESSEEEEEDGESETHDGTVAVSDIPRLIPTGAPGSNEQYNVGMVGTHGLETSHADSFSGSISREGTLMIRETSGEKKRSGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHSQEMDSGTEYGMGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW DIP protein Q9UKE5 original sequence version: 153 DIP protein Q9UKE5 original sequence version: 119 DIP protein Q9UKE5-1 original sequence version: 119 3590BC2905A72C3D rap2a_human Ras-related protein Rap-2a RbBP-30 RAP2A protein protein human Homo sapiens Human MREYKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYEKVPVILVGNKVDLESEREVSSSEGRALAEEWGCPFMETSAKSKTMVDELFAEIVRQMNYAAQPDKDDPCCSACNIQ 047D49762765F0B7 d3z811_rat Tnik protein protein rat Rattus norvegicus (Rat) Rat MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHGQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPLEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPNLPPRSESFSISGVQPARTPPMLRPVDPQIPQLVAVKSQGPALTASQSVHEQPTKGLSGFQEALNVTSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGSALGPRLGSQPIRASNPDLRRTEPVLESSLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESRDIPRPSRPADLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSEEEEEDGESETHDGTVAVSDIPRLIPTGAPGKNEQYNMGMVGTHGLETSHADTFGGSISREGTLMIRETAEEKKRSGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHSQEMDTGAEYGMGSSTKASFTPFVDPRVYQTSPTDEDEEDDESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW The sequence has been withdrawn from uniprot. 934F9F28B8136797 tanc1_rat Protein TANC1 Tanc1 Tanc TPR domain, ankyrin-repeat and coiled-coil domain-containing protein 1 protein protein rat Rattus norvegicus (Rat) Rat MLKAVLKKSREGVKGSKKEAGGDFGSETPTLSSSGDSPVNSLSTTEDTYRVSLAKGVSMSLPSSPLLPRQSHLTQSRANKKSPGPVRKPKYVESPRVPGDPVMIPFREGSKPAEPIETEAKVDNEPSCSPAAQELLTRLGFLLGEGIPSATHITIEDKNEAMCTALSQGISPCSTLTSSTASPSTDSPCSTLNSCVSKTAANKSPCETISSPSSTLESKDSGIIATITSSSENDDRSGSSLEWNRDGSLRLGVQKGVLHDRRLDNCSPVAEEETTGSAESVLPKAESSAGDGPVPYSQSSGSLIMPRPNSVAATSSTKLEDLSYLDGQRNAPLRTSIRLPWHSTAGARFAPYKPQEILLKPLLFEVPSITTDSVFVGRDWLFQQIEENLRNTELAENRGAVVVGNVGFGKTAIISKLVALSCHGSRMRQVASSSPSSSLKTSDPTHDLPGTPLLSPSSSTSALSAARTPPGPGTVDSQRPREDAVKYLASRVVAYHYCQADNTYTCLVPEFVHSIATLLCRSHQLAAYRDLLIREPQLQSMLNLRSCVQDPVAAFKRGILEPLTNLRNEQKIPEEEYIILIDGLNEAEFHKPDHGDTLSSFITKIIPKFPPWLKLIVTVRADFQEIISTLPFVKLSLDDFPGNQDIHSDLHAYVQHRVHSSQDILSNISLNGKADAALIGKVSSRLVLRNLGSYLYLKLTLDLFQRGHLVIKSASYKVVPVSLSELYLLQCNMKFMTQSAFDRALPILNVALASLHPMTDEQIFQAINAGHIQGEQGWEDFQQKMEALSCFLIKKRDKTRMFCHPSFREWLVWRADGESTAFLCEPRNGHALLAFMFSRQESKLNRQQTVELGHHILKAHIFKGLSKKTGVSSSHPQALWIGYSTEGLSAALASLRNLYTPNVKVSRLLILGGANVNYRTEVLNNAPILCVQSHLGHEEVVTLLLEFGACLDGMSENGMNALCYAAAAGHMKLVCLLTKKGARVDHLDKKGQCALVHSALRGHSDILQYLLNCEWSAGPPQPGTLRKSQALQQALTAAASMGHSAVVQSLLGMAEEHEIEVNGTDTLWGETALTAAAGRGKLEICELLLERGAAVSRANRRGVPPLFCAARQGHWQVVQLLLDRGCDVNPNDKQGRTPLMVAACEGHLSTVEFLLSKGAALSSLDKEGLSALSWACLKGHRAVVQYLVEEGAEIDQTDKNGRTPLDLAAFYGDAETVLYLVEKGAVIEHVDHSGMRPLDRAIGCRNTAVVVTLLRKGAKLGNAAWAMATFKPDILIILLQKLMEEGNVMYKKGKMKEAAQRYQYALRKFPREGPGEDMRPFNELRVSLYLNLSRCRRKTNDFGLAEEFASKALELKPKSYEAFYARARAKRNSRQFLAALADLQEAVKLCPTNQEIKRLLARVEEECKQLQRNQQQKQQAPLPAPPNDSDNDEEAPASSLKDHFPIEEAEEEDTSSQEESISPTPRSQPPPSVPSPYIRNLQEGLQSKGRPASPQSWAGISKSLRETVAQPGLVMQPTKQAQIVKTNQHLGSGQSSMRNSNTKVQVSSQNPPPSPMPGRVSAAPAVSRNQHLEGTGPFSTGTGCGHFGDRLGPSQSLQLQRGESGTAYPLPSKVKAAERLLAHASVAVDMALPSQGGPVSCSDVRHPASLSSSGSSGSPSSSVKMSSSTSSLTSSSSVSDGFKAQGPDCRIRDRGTTQVQGGTAEHRPRNTPFMGIMDKIARFQQQVNPPSRSWHCPVTEGLLTNTATAAGLQTNSEKPALKPGGYCSQAKPCSVPPLGMGVHNGAQVKELEENKCQVPALCQDNRKTKGVPHLYPEGVSKQPLHVSTEAHRSHLTSAKPKRSFIESNV E52BA9EABF2ECECF mink1_mouse Misshapen-like kinase 1 Mitogen-activated protein kinase kinase kinase kinase 6 MAPK/ERK kinase kinase kinase 6 Misshapen/NIK-related kinase GCK family kinase MiNK Mink1 Map4k6 Mink protein protein mouse Mus musculus Mouse MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW 455E546EC5459B47 mink1_human Misshapen-like kinase 1 GCK family kinase MiNK Misshapen/NIK-related kinase MINK1 MAP4K6 MINK Mitogen-activated protein kinase kinase kinase kinase 6 MAPK/ERK kinase kinase kinase 6 B55 YSK2 ZC3 protein protein human Homo sapiens Human MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQLLPGDRKPLYHYGRGMNPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAIPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGVSSKPDSSPVLSPGNKAKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDDEEEGEGGPAEGSRDTPGGRSDGDTDSVSTMVVHDVEEITGTQPPYGGGTMVVQRTPEEERNLLHADSNGYTNLPDVVQPSHSPTENSKGQSPPSKDGSGDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDSIPITALVGGEGTRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW 3E3B182CDDB3659C rap2a-mink1-1 1 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association By the affinity chromatography/mass-spectrometry approach and the yeast two-hybrid screening, we identified Misshapen/NIKs-related kinase (MINK) as a novel Rap2-interacting protein Figs 1A and 1B Interaction required the presence of GTP rap2a-mink1-2 3 8 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association We screened a human fetal brain cDNA library using hRap2A as bait and isolated two hMINK clones (pACT2-hMINK-A and -B) Fig 1B Fig 1B suggests the length of MINK1 is 1354 but the longest isoform in UniProtKB 1332 amino acids. The length of the sequenced prey has been amended to match the UniProtKB entry. rap2a-mink1-3 2 8 unspecified role unspecified role bait bait P10114:p.Ser17Asn mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S N Wed May 02 17:35:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser17asn' Tue Jun 30 19:53:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser17Asn' P10114:p.Phe39Ser mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F S Wed May 02 17:48:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe39ser' Tue Jun 30 18:50:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe39Ser' 12 unspecified role unspecified role prey prey physical association physical association We screened a human fetal brain cDNA library using hRap2A as bait and isolated two hMINK clones (pACT2-hMINK-A and -B) Fig 1C rap2a-mink1-4 4 11 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag mutation analysis mutation analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P10114:p.Phe39Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F S Wed May 02 16:22:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe39ser' Tue Jun 30 18:35:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe39Ser' colocalization colocalization Strikingly, in coexpression experiments, MINK colocalized with most of the vesicular structures containing Rap2 or Rap2(G12V). Fig 1F tnik1-tnac1-1 1 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 10 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] physical association physical association A double band of 200 kDa (p200) was observed in eluates from the GST-hTNIK1(279-1052) and -hTNIK1(753-1360) columns. LC-MS/MS analysis of p200 identified nine peptides matching rat TANC1 (rTANC1), the calculated molecular mass of which is 200,376 Da. (Fig. 2B). Fig. 2B tnik1-tnac1-2 5 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 10 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] physical association physical association Fig. 2A tnik1-tnac1-3 1 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tpr_coil coil region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] 9 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] physical association physical association Both MINK and TNIK were found in the eluate from the GST-TPRcc column, but not in the eluates from the other columns (Fig. 2C). Fig. 2C