UniProt UniProt http://www.uniprot.org The Polo-like kinase Cdc5 interacts with FEAR network components and Cdc14. The Polo-like kinase Cdc5 interacts with FEAR network components and Cdc14. Cell Cycle (1538-4101) 2008 imex curation Rahal R., Amon A. angelika@mit.edu 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Rahal R., Amon A. angelika@mit.edu Accepted 2009-FEB-20 by JYOTI. 03-Feb-2009: Contacted by JYOTI. Cell Cycle (1538-4101) 2008 jyoti Only protein-protein interactions imex curation The Polo-like kinase Cdc5 interacts with FEAR network components and Cdc14. The Polo-like kinase Cdc5 interacts with FEAR network components and Cdc14. Cell Cycle (1538-4101) 2008 imex curation Rahal R., Amon A. angelika@mit.edu 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rahal R., Amon A. angelika@mit.edu Accepted 2009-FEB-20 by JYOTI. 03-Mar-2009: Contacted by JYOTI. Cell Cycle (1538-4101) 2008 jyoti Only protein-protein interactions imex curation cdc14_yeast Tyrosine-protein phosphatase CDC14 CDC14 OAF3 YFR028C protein protein yeast Saccharomyces cerevisiae Baker's yeast MRRSVYLDNTIEFLRGRVYLGAYDYTPEDTDELVFFTVEDAIFYNSFHLDFGPMNIGHLYRFAVIFHEILNDPENANKAVVFYSSASTRQRANAACMLCCYMILVQAWTPHQVLQPLAQVDPPFMPFRDAGYSNADFEITIQDVVYGVWRAKEKGLIDLHSFNLESYEKYEHVEFGDFNVLTPDFIAFASPQEDHPKGYLATKSSHLNQPFKSVLNFFANNNVQLVVRLNSHLYNKKHFEDIGIQHLDLIFEDGTCPDLSIVKNFVGAAETIIKRGGKIAVHCKAGLGRTGCLIGAHLIYTYGFTANECIGFLRFIRPGMVVGPQQHWLYLHQNDFREWKYTTRISLKPSEAIGGLYPLISLEEYRLQKKKLKDDKRVAQNNIEGELRDLTMTPPSNGHGALSARNSSQPSTANNGSNSFKSSAVPQTSPGQPRKGQNGSNTIEDINNNRNPTSHANRKVVIESNNSDDESMQDTNGTSNHYPKVSRKKNDISSASSSRMEDNEPSATNINNAADDTILRQLLPKNRRVTSGRRTTSAAGGIRKISGSIKK 4EB3985DFA3FD823 4EB3985DFA3FD823 slk19_yeast Kinetochore protein SLK19 Synthetic lethal KAR3 protein 19 SLK19 YOR195W protein protein yeast Saccharomyces cerevisiae Baker's yeast MNEVPTTPVRLILGQAQQREQNSENCSQERNPRTFNSEPDSSFNSPGSSQFVIHPHEPLEKEKDEKQDLDRSIDYGRSSALNNKNNANPLENIDINKMFDDKKSDSGTNDDKGGASTSDKHVLALNYSPIRVEMNSSEKRSDKNVDVDENDKEGSHINKKLKLQLESVPDLKQSSTKDIINDKEEIMSSPMAIDMIETNISPNKFIINDGVERNDSFNINTDTLKLENDINEKQQEEDFIKSNSNNVVNIDNAYKEKEDEENDITNSHINRLTPLYETSARESNSNEEGRNDYDDDNQLDIRHDNFQIVAKRNEELTDQIYHLNQMLNSLISKNESLSFQYEKLNKNHQLLIDLTNEKLDKLNTERESDIAKVEKFKKRIKELNTEIKVLNSNQKILQEKFDASITEVNHIKGEHENTVNTLQQNEKILNDKNVELENMKAELKGNNDKLSEYETTLNDLNSRIVQLNDKIESTDIVLKSKENELDNLKLSLKETLSISKDFNDSDLIGQINELISTKNNLQQKMDDLNNLNDDNLKVVQDKLIKNEETLKLKEAEIDSLNSEMDELKKQITSKDDEFKMWQSKYETVEDEAKIRNAEVTELNGDIEDLKESKLHLEETITELENKVHKLENECELEKQKFEKTSLELESLQLKNSNIQAEHIKELENLHENLISLQNELKISSDRITTLTKENEVLMEQNNNNNNSVTLSNDQKDRDDEKIKSLGKQVQDWKEKYEAKEKDTNKRLKLLAEDLYIQYSSKHEQKVKLLKKGYENKYQNKFDQLNLENKTLSEEIEQLNKQLSSEREEKQELLKLLENEKK 994CCE2EFB7C4B67 cdc5_yeast Cell cycle serine/threonine-protein kinase CDC5/MSD2 CDC5 MSD2 PKX2 YMR001C YM8270.03C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLGPLKAINDKQLNTRSKLVHTPIKGNTADLVGKENHFKQTKRLDPNNDHHHQPAQKKKREKLSALCKTPPSLIKTRGKDYHRGHFLGEGGFARCFQIKDDSGEIFAAKTVAKASIKSEKTRKKLLSEIQIHKSMSHPNIVQFIDCFEDDSNVYILLEICPNGSLMELLKRRKVLTEPEVRFFTTQICGAIKYMHSRRVIHRDLKLGNIFFDSNYNLKIGDFGLAAVLANESERKYTICGTPNYIAPEVLMGKHSGHSFEVDIWSLGVMLYALLIGKPPFQARDVNTIYERIKCRDFSFPRDKPISDEGKILIRDILSLDPIERPSLTEIMDYVWFRGTFPPSIPSTVMSEAPNFEDIPEEQSLVNFKDCMEKSLLLESMSSDKIQRQKRDYISSIKSSIDKLEEYHQNRPFLPHSLSPGGTKQKYKEVVDIEAQRRLNDLAREARIRRAQQAVLRKELIATSTNVIKSEISLRILASECHLTLNGIVEAEAQYKMGGLPKSRLPKIKHPMIVTKWVDYSNKHGFSYQLSTEDIGVLFNNGTTVLRLADAEEFWYISYDDREGWVASHYLLSEKPRELSRHLEVVDFFAKYMKANLSRVSTFGREEYHKDDVFLRRYTRYKPFVMFELSDGTFQFNFKDHHKMAISDGGKLVTYISPSHESTTYPLVEVLKYGEIPGYPESNFREKLTLIKEGLKQKSTIVTVD B5A25F1BBBEAA3DC B5A25F1BBBEAA3DC esp1_yeast Separin ESP1 YGR098C Separase protein protein yeast Saccharomyces cerevisiae Baker's yeast MMVKQEEPLNEISPNTPMTSKSYLLNDTLSKVHHSGQTRPLTSVLSGDASSNSIGILAMHNNIIRDFTKIASNNIDLAIEDITTVDHSLNSIYSLLKSHHMWGHINSTVKQHLMIIVKLINNNALGLASSEIIFLFNETNLFQAHSLKNILLADFSTWNDYYLSNLKILALQIILKRKLVDEYLPHILELFSHDKRYLLKDPNLKAHALTKIVLSFFSVTTSCKVLFGLKFLQYIKQFKLPFKKFISNITVECFSKNLLHKNYLEMGPNKIYLNSFYLSYSMLYDGLDKIMLLDILSYEETTEVQRAIKSKKEFNEYCNMSENRLLWSCISVDDLNVILENATNFLQNKGKHISATLKCLVCLWSTIRLEGLPKNKDILRQFDCTVIYINSNIKSINDESAAALLSELLGVLSEICIDYKEPKRLSNIISVLFNASVLFKSHSFLLKTANLEISNVLISNDSKTSHRTILKFEKFISSAQSAQKKIEIFSCLFNVYCMLRNDTLSFVFDFCQNAFIHCFTRLKITKFIEFSNSSEIMLSVLYGNSSIENIPSENWSQLSRMIFCSLRGIFDLDPLELNNTFDKLHLLNKYELLIRIVYLLNLDMSKHLTTNLSKITKLYINKWLQKSDEKAERISSFEMDFVKMLLCYLNFNNFDKLSIELSLCIKSKEKYYSSIVPYADNYLLEAYLSLYMIDDALMMKNQLQKTMNLSTAKIEQALLHASSLINVHLWDSDLTAFQIYFGKTLPAMKPELFDINNDHNLPMSLYIKVILLNIKIFNESAKLNIKAGNVISAVIDCRKAQNLALSLLKKKNKLSQGSRLALLKSLSFSFFQLIKIHIRIGSARDCEFYSKELSRIISDLEEPIIVYRCLHFLHRYYMITEQTCLQNITLGKANKAFDYLDAEADITSLTMFLYDNKEFVKLEQSLVLYFGDQLEKTFLPNLWKLHLGKDIDDSICLSEYMPKNVINRVHNMWQKVMSQLEEDPFFKGMFESTLGIPSSLPVIPSTMPNNILKTPSKHSTGLKLCDSPRSSSMTPRGKNIRQKFDRIAAISKLKQMKELLESLKLDTLDNHELSKISSLSSLTLTILSNITSIHNAESSLITNFSLTDLPRHMPLLFDKVLNNIDNKNYREFRVSSLIAPNNISTITESIRVSAAQKDLMESNLNINVITIDFCPITGNLLLSKLEPRRKRRTHLRLPLIRSNSRDLDEVHLSFPEATKKLLSIINESNQTTSVEVTNKIKTREERKSWWTTRYDLDKRMQQLLNNIENSWFNGVQGFFSPEVVDNSLFEKFKDKFYEILHQNLPSRKLYGNPAMFIKVEDWVIELFLKLNPQEIDFLSKMEDLIYFVLDILLFHGEENAYDEIDFSMLHVQLEEQIKKYRATMTTNSIFHTFLVVSSSCHLFPWECLSFLKDLSITRVPSYVCLNKLLSRFHYQLPLQVTIEDNISMILNPNGDLSRTESKFKGMFQKIIDAKPSSQLVMNEKPEEETLLKMLQNSNLFVYIGHGGGEQYVRSKEIKKCTKIAPSFLLGCSSAAMKYYGKLEPTGTIYTYLLGGCPMVLGNLWDVTDKDIDKFSEELFEKMGFRCNTDDLNGNSLSVSYAVSKSRGVCHLRYLNGAAPVIYGLPIKFVS FBAFD33FF706C6BA slk19-esp1-1 2 6 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role bait bait c-terminus protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 1A, 1B, 1C, 2E Interaction occurs in M-phase. cdc5-esp1-1 2 6 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role bait bait c-terminus protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 2A, 2C, 2E Interaction occurs in M-phase. cdc5-slk19-1 2 5 unspecified role unspecified role bait bait c-terminus protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 2B, 2D, 2F Interaction occurs in M-phase. cdc5-cdc14-1 2 3 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role bait bait c-terminus protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 3A, 3B, 3C Interaction occurs in M-phase. cdc5-cdc14-2 1 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P32562:p.[His641Ala;Lys643Met] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain K M Wed May 02 18:05:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his641ala,lys643met' Tue Jun 30 15:43:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His641Ala;Lys643Met]' n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig 4A, 4B, 4D, 4E, 4F