IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III. Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III. Mutat. Res. (0027-5107) 2008 imex curation Li F., Liu Q., Chen YY., Yu ZN., Zhang ZP., Zhou YF., Deng JY., Bi LJ., Zhang XE. blj@sun5.ibp.ac.cn 29-Mar-2010: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting tag visualisation tag visualisation Li F., Liu Q., Chen YY., Yu ZN., Zhang ZP., Zhou YF., Deng JY., Bi LJ., Zhang XE. blj@sun5.ibp.ac.cn Accepted 2010-MAR-25 by ORCHARD. 29-Mar-2010: Contacted by JYOTI. Mutat. Res. (0027-5107) 2008 orchard Only protein-protein interactions imex curation Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III. Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III. Mutat. Res. (0027-5107) 2008 imex curation Li F., Liu Q., Chen YY., Yu ZN., Zhang ZP., Zhou YF., Deng JY., Bi LJ., Zhang XE. blj@sun5.ibp.ac.cn 29-Mar-2010: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Li F., Liu Q., Chen YY., Yu ZN., Zhang ZP., Zhou YF., Deng JY., Bi LJ., Zhang XE. blj@sun5.ibp.ac.cn Accepted 2010-MAR-25 by ORCHARD. 29-Mar-2010: Contacted by JYOTI. Mutat. Res. (0027-5107) 2008 orchard Only protein-protein interactions imex curation hola_ecoli DNA polymerase III subunit delta b0640 holA JW0635 protein protein ecoli Escherichia coli (strain K12) MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAAQGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTGLLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNLELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWVDALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKHRVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHKPLADVFIDG 87239A678FE16BE2 87239A678FE16BE2 p06710-2 DNA polymerase III subunit tau dnaX dnaZ dnaZX b0470 JW0459 DNA polymerase III subunit gamma protein protein ecoli Escherichia coli (strain K12) MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKE Formed by programmed ribosomal frameshifting to a premature stop codon in the -1 frame at codon 430, the last residue is thus Glu and not Ser. Mutants which remove the frameshift are viable, suggesting strongly that gamma is not essential for viability (Ref.14). 8177A07F31BC86E3 uvrd_ecoli DNA helicase II uvrD mutU pdeB rad recL b3813 JW3786 protein protein ecoli Escherichia coli (strain K12) MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQSYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV DIP protein P03018 original sequence version: 136 DIP protein P03018 original sequence version: null FA68E7267C77B49A holb_ecoli DNA polymerase III subunit delta' holB b1099 JW1085 protein protein ecoli Escherichia coli (strain K12) MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYALSRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGVDAVREVTEKLNEHARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCRLHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAELANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL DIP protein P28631 original sequence version: 95 DIP protein P28631 original sequence version: null DC9DAA644AD8A096 mutl_ecoli DNA mismatch repair protein MutL mutL b4170 JW4128 protein protein ecoli Escherichia coli (strain K12) MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDIDIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASISSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKFLRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGTAFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQACEDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQLETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPNAQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLERDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALAELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLMSEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE DIP protein P23367 original sequence version: 102 DIP protein P23367 original sequence version: null C69D2735BF5FA165 mutl_hola 1 7 unspecified role unspecified role bait bait c terminal streptavidin-binding peptide MDEKTTGWRGGHVVEGLAGELEQLRAR LEHHPQGQREP tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c terminal linker peptide (Gly3-Ser3-Gly3)3 tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association Fig. 2. Far western analysis between MutL and subunits delta, delta prime and gamma. mutl_holb 1 6 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait c terminal streptavidin-binding peptide MDEKTTGWRGGHVVEGLAGELEQLRAR LEHHPQGQREP tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c terminal linker peptide (Gly3-Ser3-Gly3)3 tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 2. Far western analysis between MutL and subunits delta, delta prime and gamma. mutl_dnax 1 4 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait c terminal streptavidin-binding peptide MDEKTTGWRGGHVVEGLAGELEQLRAR LEHHPQGQREP tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region linker peptide (Gly3-Ser3-Gly3)3 tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 2. Far western analysis between MutL and subunits delta, delta prime and gamma. mutl_uvrd 1 5 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait region linker peptide (Gly3-Ser3-Gly3)3 tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c terminal streptavidin-binding peptide MDEKTTGWRGGHVVEGLAGELEQLRAR LEHHPQGQREP tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 2. UvrD is known to interact with MutL mutl_uvrd-1 2 7 unspecified role unspecified role bait bait c terminal streptavidin-binding peptide MDEKTTGWRGGHVVEGLAGELEQLRAR LEHHPQGQREP tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region linker peptide (Gly3-Ser3-Gly3)3 tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3. SPR analysis confirmed the interactions between MutL and subunits delta, delta' and gamma. mutl_dnax-1 2 4 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region linker peptide (Gly3-Ser3-Gly3)3 tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c terminal streptavidin-binding peptide MDEKTTGWRGGHVVEGLAGELEQLRAR LEHHPQGQREP tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3. SPR analysis confirmed the interactions between MutL and subunits delta, delta' and gamma. pH=7.5 mutl_holb-1 2 6 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey c terminal streptavidin-binding peptide MDEKTTGWRGGHVVEGLAGELEQLRAR LEHHPQGQREP tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3. SPR analysis confirmed the interactions between MutL and subunits delta, delta' and gamma. pH=7.5 mutl_hola-1 2 3 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait c terminal streptavidin-binding peptide MDEKTTGWRGGHVVEGLAGELEQLRAR LEHHPQGQREP tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region linker peptide (Gly3-Ser3-Gly3)3 t tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3. SPR analysis confirmed the interactions between MutL and subunits delta, delta' and gamma. pH=7.5