IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A protein interaction network for pluripotency of embryonic stem cells. A protein interaction network for pluripotency of embryonic stem cells. Nature (0028-0836) 2006 imex curation Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu Accepted 2009-MAY-21 by DAVET. 27-Oct-2007: Contacted by JYOTI. Nature (0028-0836) 2006 davet Only protein-protein interactions imex curation A protein interaction network for pluripotency of embryonic stem cells. A protein interaction network for pluripotency of embryonic stem cells. Nature (0028-0836) 2006 imex curation Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells pull down pull down western blot western blot Immuno blot Immuno blot Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu Accepted 2009-MAY-21 by DAVET. 27-Oct-2007: Contacted by JYOTI. Nature (0028-0836) 2006 davet Only protein-protein interactions imex curation A protein interaction network for pluripotency of embryonic stem cells. A protein interaction network for pluripotency of embryonic stem cells. Nature (0028-0836) 2006 imex curation Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells pull down pull down fingerprinting peptide massfingerprinting fingerprinting Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu Accepted 2009-MAY-21 by DAVET. 27-Oct-2007: Contacted by JYOTI. Nature (0028-0836) 2006 davet Only protein-protein interactions imex curation A protein interaction network for pluripotency of embryonic stem cells. A protein interaction network for pluripotency of embryonic stem cells. Nature (0028-0836) 2006 imex curation Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu Accepted 2009-MAY-21 by DAVET. 27-Oct-2007: Contacted by JYOTI. Nature (0028-0836) 2006 davet Only protein-protein interactions imex curation A protein interaction network for pluripotency of embryonic stem cells. A protein interaction network for pluripotency of embryonic stem cells. Nature (0028-0836) 2006 imex curation Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu Accepted 2009-MAY-21 by DAVET. 27-Oct-2007: Contacted by JYOTI. Nature (0028-0836) 2006 davet Only protein-protein interactions imex curation A protein interaction network for pluripotency of embryonic stem cells. A protein interaction network for pluripotency of embryonic stem cells. Nature (0028-0836) 2006 imex curation Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu Accepted 2009-MAY-21 by DAVET. Nature (0028-0836) 2006 davet Only protein-protein interactions imex curation A protein interaction network for pluripotency of embryonic stem cells. A protein interaction network for pluripotency of embryonic stem cells. Nature (0028-0836) 2006 imex curation Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu Accepted 2009-MAY-21 by DAVET. 27-Oct-2007: Contacted by JYOTI. Nature (0028-0836) 2006 davet Only protein-protein interactions imex curation A protein interaction network for pluripotency of embryonic stem cells. A protein interaction network for pluripotency of embryonic stem cells. Nature (0028-0836) 2006 imex curation Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Wang J., Rao S., Chu J., Shen X., Levasseur D.N., Theunissen T.W., Orkin S.H. A tandem purification was carried out with an anti-Flag immunoprecipitation followed by capture with streptavidin. stuart_orkin@dfci.harvard.edu Accepted 2009-MAY-21 by DAVET. 27-Oct-2007: Contacted by JYOTI. Nature (0028-0836) 2006 davet Only protein-protein interactions imex curation ilf2_mouse Interleukin enhancer-binding factor 2 Ilf2 Nf45 Nuclear factor of activated T-cells 45 kDa protein protein mouse Mus musculus Mouse MRGDRGRGRGGRFGSRGGPGGGFRPFVPHIPFDFYLCEMAFPRVKPAPDETSFSEALLKRNQDLAPNSAEQASILSLVTKINNVIDNLIVAPGTFEVQIEEVRQVGSYKKGTMTTGHNVADLVVILKILPTLEAVAALGNKVVESLRAQDPSEVLTMLTNETGFEISSSDATVKILITTVPPNLRKLDPELHLDIKVLQSALAAIRHARWFEENASQSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILAAGLFLPGSVGITDPCESGNFRVHTVMTLEQQDMVCYTAQTLVRILSHGGFRKILGQEGDASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEEEENTEEPPQGEEEESMETQE 75BAD022DCD4EE01 sall4_mouse Sal-like protein 4 Sall4 Zinc finger protein SALL4 protein protein mouse Mus musculus Mouse MSRRKQAKPQHINWEEGQGEQPQQLPSPDLAEALAAEEPGAPVNSPGNCDEASEDSIPVKRPRREDTHICNKCCAEFFSLSEFMEHKKSCTKTPPVLIMNDSEGPVPSEDFSRAALSHQLGSPSNKDSLQENGSSSGDLKKLGTDSILYLKTEATQPSTPQDISYLPKGKVANTNVTLQALRGTKVAVNQRGAEAPMAPMPAAQGIPWVLEQILCLQQQQLQQIQLTEQIRVQVNMWAAHALHSGVAGADTLKALSSHVSQQVSVSQQVSAAVALLSQKASNPALSLDALKQAKLPHASVPSAASPLSSGLTSFTLKPDGTRVLPNFVSRLPSALLPQTPGSVLLQSPFSAVTLDQSKKGKGKPQNLSASASVLDVKAKDEVVLGKHKCRYCPKVFGTDSSLQIHLRSHTGERPYVCPICGHRFTTKGNLKVHLQRHPEVKANPQLLAEFQDKGAVSAASHYALPVPVPADESSLSVDAEPVPVTGTPSLGLPQKLTSGPNSRDLMGGSLPNDMQPGPSPESEAGLPLLGVGMIHNPPKAGGFQGTGAPESGSETLKLQQLVENIDKATTDPNECLICHRVLSCQSSLKMHYRTHTGERPFQCKICGRAFSTKGNLKTHLGVHRTNTTVKTQHSCPICQKKFTNAVMLQQHIRMHMGGQIPNTPLPESPCDFTAPEPVAVSENGSASGVCQDDAAEGMEAEEVCSQDVPSGPSTVSLPVPSAHLASPSLGFSVLASLDTQGKGALPALALQRQSSRENSSLEGGDTGPANDSSLLVGDQECQSRSPDATETMCYQAVSPANSQAGSVKSRSPEGHKAEGVESCRVDTEGRTSLPPTFIRAQPTFVKVEVPGTFVGPPSMPSGMPPLLASQPQPRRQAKQHCCTRCGKNFSSASALQIHERTHTGEKPFVCNICGRAFTTKGNLKVHYMTHGANNNSARRGRKLAIENPMAALSAEGKRAPEVFSKELLSPAVSVDPASWNQYTSVLNGGLAMKTNEISVIQSGGIPTLPVSLGASSVVSNGTISKLDGSQTGVSMPMSGNGEKLAVPDGMAKHQFPHFLEENKIAVS DIP protein Q8BX22 original sequence version: 49 DIP protein Q8BX22 original sequence version: 66 DIP protein Q8BX22 original sequence version: 55 23B92F488AAF53E5 q8ve57_mouse Wfdc5 protein protein mouse Mus musculus Mouse MRVQSSLLLVVLLALETQLPVALCRKKGDKLGGCPPDDGLCNQNTPPDQCLNDKQCPSSWKCCRRACFLQCMPRVFVKSGKCPVDQLRCLSPTKHMCNKDSDCSGKKRCCASACGRDCRDPSKSTI 8D10353557B2F75E err2_mouse Steroid hormone receptor ERR2 Esrrb Err-2 Err2 Nr3b2 Estrogen-related receptor beta Estrogen receptor-like 2 Nuclear receptor subfamily 3 group B member 2 protein protein mouse Mus musculus Mouse MSSEDRHLGSSCGSFIKTEPSSPSSGIDALSHHSPSGSSDASGGFGIALSTHANGLDSPPMFAGAGLGGNPCRKSYEDCTSGIMEDSAIKCEYMLNAIPKRLCLVCGDIASGYHYGVASCEACKAFFKRTIQGNIEYNCPATNECEITKRRRKSCQACRFMKCLKVGMLKEGVRLDRVRGGRQKYKRRLDSENSPYLNLPISPPAKKPLTKIVSNLLGVEQDKLYAMPPNDIPEGDIKALTTLCELADRELVFLINWAKHIPGFPSLTLGDQMSLLQSAWMEILILGIVYRSLPYDDKLAYAEDYIMDEEHSRLVGLLDLYRAILQLVRRYKKLKVEKEEFMILKALALANSDSMYIENLEAVQKLQDLLHEALQDYELSQRHEEPRRAGKLLLTLPLLRQTAAKAVQHFYSVKLQGKVPMHKLFLEMLEAKV DIP protein Q61539 original sequence version: 70 1A0383E7E2ED8BF3 sp1_mouse Transcription factor Sp1 Sp1 Specificity protein 1 protein protein mouse Mus musculus Mouse MSDQDHSMDEVTAVVKIEKDVGGNNGGSGNGGGAAFSQTRSSSTGSSSSSGGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGNSTNGSNGSESSKNRTVSGGQYVVAATPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIIPNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAGTISSSGSQESSSQPVTSGTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTSSGSSGTSSQGQTPQRVGGLQGSDSLNIQQNQTSGGSLQGSQQKEGEQSQQTQQQQILIQPQLVQGGQALQALQAAPLSGQTFTTQAISQETLQNLQLQAVQNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHQLPGLPLAIANTPGDHGTQLGLHGSGGDGIHDETAGGEGENSSDLQPQAGRRTRREACTCPYCKDSEGRASGDPGKKKQHICHIQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSYCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVTELQSINISGNGF DIP protein O89090 original sequence version: 112 B6B989F0A252CEE5 mbb1a_mouse Myb-binding protein 1A Mybbp1a P160 Myb-binding protein of 160 kDa protein protein mouse Mus musculus Mouse MAEMKSPTKAEPATPAEAAQSDRHSLLEHSREFLDFFWDIAKPDQETRLRATEKLLEYLRTRPNDSEMKYALKRLITGLGVGREAARPCYSLALAQLLQSFEDIPLCDILDQIQEKYSLQAMNKAMMRPSLFANLFGVLALFQSGRLVKDKEALMKSVQLLKILSQHPNHLQGQPIKALVDILSEVPESMFQEILPKVLKGNMKVILRSPKYLELFLLAKQRVPTKLESLMGSVDLFSEDNIPSLVNILKVAANSVKKEHKLPNVALDLLRLALKESRFELFWKKVLEEGLLKNPSWTSSYMCFRLLGASLPLLSEEQLQLVMRGDLIRHFGENMVISKPQNLFKIIPEISTYVGTFLEGCQDDPKRQLTMMVAFTTITNQGLPVMPTFWRVTRFLNAEALQSYVAWLRDMFLQPDLNSLVDFSTANQKRAQDASLNVPERAVFRLRKWIIHRLVSLVDHLHLEKDEAVVEQIARFCLFHAFFKTKKATPQIPETKQHFSFPLDDRNRGVFVSAFFSLLQTLSVKFRQTPDLAENGKPWTYRLVQLADMLLNHNRNVTSVTSLTTQQRQAWDQMMSTLKELEARSSETRAIAFQHLLLLVGLHIFKSPAESCDVLGDIQTCIKKSMEQNPRRSRSRAKASQEPVWVEVMVEILLSLLAQPSNLMRQVVRSVFGHICPHLTPRCLQLILAVLSPVTNEDEDDNVVVTDDADEKQLQHGEDEDSDNEDNKNSESDMDSEDGEESEEEDRDKDVDPGFRQQLMEVLKAGNALGGVDNEEEEELGDEAMMALDQNLASLFKEQKMRIQARNEEKNKLQKEKKLRRDFQIRALDLIEVLVTKQPEHPLILELLEPLLNVIQHSMRSKGSTKQEQDLLHKTARIFMHHLCRARRYCHEVGPCAEALHAQVERLVQQAGSQADASVALYYFNASLYLLRVLKGNTNKRHQDGHKLHGADTEDSEDQAANCLDLDFVTRVYSASLESLLTKRNSSLTVPMFLSLFSRYPVICKNLLPVLAQHVAGPSRPRHQAQACLMLQKTLSARELRVCFEDPEWEQLITQLLGKATQTLQTLGEAQSKGEHQKELSILELLNTLLRTVNHEKLSVDLTAPLGVLQSKQQKLQQSLQQGNHSSGSNRLYDLYWQAMRMLGVQRPKSEKKNAKDIPSDTQSPVSTKRKKKGFLPETKKRKKLKSEGTTPEKNAASQQDAVTEGAMPAATGKDQPPSTGKKKRKRVKASTPSQVNGITGAKSPAPSNPTLSPSTPAKTPKLQKKKEKLSQVNGATPVSPIEPESKKHHQEALSTKEVIRKSPHPQSALPKKRARLSLVSRSPSLLQSGVKKRRVASRRVQTP DIP protein Q7TPV4 original sequence version: 72 64D3420981CD0767 zn281_mouse Zinc finger protein 281 Znf281 Zfp281 protein protein mouse Mus musculus Mouse MKIGSGFLSGGGGPSSSGGSGSGGSSGSASGGSGGGRRAEMEPTFPQSMVMFNHRLPPVTSFTRPAGTAAPPPQCVLSSSTSAAPAAEPPPPPAPDMTFKKEPAASAAAFPSQRTSWGFLQSLVSIKQEKPADPEEQPSHHHHHHHHYGGLFAGAEERSPGLGGGEGGSHGVIQDLSLLHQHAQQQPAQHHRDVLLSSGSRTDEHGNQEPKQDANVKKAKRPKPESQGIKAKRKPSASSKPLVGEGEGAVLSPSQKPHICDHCSAAFRSSYHLRRHVLIHTGERPFQCSQCSMGFIQKYLLQRHEKIHSREKPFGCDQCSMKFIQKYHMERHKRTHSGEKPYKCDTCQQYFSRTDRLLKHRRTCGEAIAKGAASAEPGSSNHNSMGNLAVLSQGNTSSSRRKSKSKSIAIENKEHKTGKTNESQMSNNINMQSYSVEMPTVSTSGSIIGTGIDELQKRVPKLIFKKGSRKNADKSYLNFVSPLPDVVGQKSLSGKPGGSLGIVSNNSVETISLLQSTSGKQGPISSNYDDAMQFSKKRRYLPTASSNSAFSINVGHMVSQQSVIQSAGVSVLDNEAPLSLIDSSALNAEIKSCHDKSGIPDEVLQSILDQYSGKSETQKEDPFNLTEPRVDLHTSGEHSELVQEENLSPGTQTPSNDKTSMLQEYSKYLQQAFEKSTNAGFTLGHGFQFVSLSSPLHNHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQQKNLESSTSFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR DIP protein Q99LI5 original sequence version: 62 7F4EBA44C2CC2AF2 zmym2_mouse Zinc finger MYM-type protein 2 Zmym2 Zfp198 Znf198 Zinc finger protein 198 protein protein mouse Mus musculus Mouse MDTSSVGTLELTDQTPVLLGSTAMATSLTNVGNSFSGPPNPLVSRSSKFQNSSVEDDDDVVFIEPVQPPPSSAPLVADQRPITFTSSKNEELQGNDPKILPSSKELAPQKGSVSETIVIDDEEDMETNQGQEKSSSNFIERRPSETKNRTNDVDFSSSTFSRSKVNAGVSNSGITTEPDSEIQIANVTTLETGVSSVSDGQLESTDGRDMNLMITHVTSLHNTSLGDGSNGLQSSNFGVNIQTYTPSLTSQTKAGVGPFNPGRMNVAGDVFQNGESAPHHNPDSWISQSASFPRNQKQQGVDSLSPVASLPKQIFQPSNQQPTKPVKVTCANCKKPLQKGQTAYQRKGSAHLFCSTTCLSSFSHKPAPKKLCVMCKKDITTMKGTIVAQVDSSESFQEFCSTSCLSLYEDKQSPAKGALNKSRCTICGKLTEIRHEVSFKNMTHKLCSDHCFNRYRMANGLIMNCCEQCGEYLPSKGAGNNVLVVDGQQKRFCCQSCVTEYKQVGSHPSFLKEVRDHMQDSFLMQPEKYGKLTTCTGCRTQCRFFDMTQCIGPNGYMEPYCSTACMNSHKTKYAKSQSLGIICHFCKRNSLPQYQATMPDGKLYNFCNSSCVAKFQALSMQSSPNGQFVAPSDIQLKCNYCKNSFCSKPEILEWENKVHQFCSKTCSDDYKKLHCIVTYCEYCQEEKTLHETVNFSGVKRPFCSEGCKLLYKQDFARRLGLRCVTCNYCSQLCKKGATKELDGVVRDFCSEDCCKKFQEWYYKAARCDCCKSQGTLKERVQWRGEMKHFCDQHCLLRFYCQQNEPNMTTQKGPENLHYDQGCQTSRTKMTGSAPPPSPTPNKEMKNKAILCKPLTMTKATYCKPHMQTKSCQTDENWKTEYVPVPIPVPVYVPVPMHMYSQNIPVPTTVPVPVPVPVFLPAPLDSSEKIPATVEDLKSKVSSDPLDSELLTMTDMMTEEEGKAEASNINSVIIETDIIGSDLTKNSDPDIQSNMPDVPYEPDLDIEIDFPRAAEELDMENEFLLPPVFGEEYEEQPRPRSKKKGTKRKAVSGYQSHDDSSDNSECSFPFKYTYGVNAWKHWVKTRQLDEDLLVLDELKSSKSVKLKEDLLSHTTAELNYGLAHFVNEIRRPNGENYAPDSIYYLCLGIQEYLCGSNRKDNIFIDPGYQMFEQELNKILRSWQPSILPDGSIFSRVEEDYLWRIKQLGSHSPVALLNTLFYFNTKYFGLKTVEQHLRLSFGTVFRHWKKNPLTMENKACLRYQVSSLCGTDNEDKIATGKRKHEDDEPVFEQVENTANPSRCPVKMFECYLSKSPQNLNQRMDVFYLQPECSSSTDSPVWYTSTSLDRNTLENMLVRVLLVKDIYDKDNYELDEDTD DIP protein Q9CU65 original sequence version: 49 1D4955422F0646B4 elys_human Protein ELYS Protein MEL-28 Putative AT-hook-containing transcription factor 1 AHCTF1 Embryonic large molecule derived from yolk sac ELYS TMBS62 MSTP108 protein protein human Homo sapiens Human MRDLRAQVTSGLLPFPEVTLQALGEDEITLESVLRGKFAAGKNGLACLACGPQLEVVNSITGERLSAYRFSGVNEQPPVVLAVKEFSWQKRTGLLIGLEETEGSVLCLYDLGISKVVKAVVLPGRVTAIEPIINHGGASASTQHLHPSLRWLFGVAAVVTDVGQILLVDLCLDDLSCNQNEVEASDLEVLTGIPAEVPHIRESVMRQGRHLCFQLVSPTGTAVSTLSYISRTNQLAVGFSDGYLALWNMKSMKREYYIQLESGQVPVYAVTFQEPENDPRNCCYLWAVQSTQDSEGDVLSLHLLQLAFGNRKCLASGQILYEGLEYCEERYTLDLTGGMFPLRGQTSNTKLLGCQSIEKFRSHGDREEGVNEALSPDTSVSVFTWQVNIYGQGKPSVYLGLFDINRWYHAQMPDSLRSGEYLHNCSYFALWSLESVVSRTSPHGILDILVHERSLNRGVPPSYPPPEQFFNPSTYNFDATCLLNSGVVHLTCTGFQKETLTFLKKSGPSLNELIPDGYNRCLVAGLLSPRFVDVQPSSLSQEEQLEAILSAAIQTSSLGLLTGYIRRWITEEQPNSATNLRFVLEWTWNKVVLTKEEFDRLCVPLFDGSCHFMDPQTIQSIQQCYLLLSNLNIVLSCFASEAREITERGLIDLSNKFVVSHLICQYAQVVLWFSHSGLLPEGIDDSVQLSRLCYNYPVIQNYYTSRRQKFERLSRGKWNPDCLMIDGLVSQLGERIEKLWKRDEGGTGKYPPASLHAVLDMYLLDGVTEAAKHSITIYLLLDIMYSFPNKTDTPIESFPTVFAISWGQVKLIQGFWLIDHNDYESGLDLLFHPATAKPLSWQHSKIIQAFMSQGEHRQALRYIQTMKPTVSSGNDVILHLTVLLFNRCMVEAWNFLRQHCNRLNIEELLKHMYEVCQEMGLMEDLLKLPFTDTEQECLVKFLQSSASVQNHEFLLVHHLQRANYVPALKLNQTLKINVMNDRDPRLRERSLARNSILDQYGKILPRVHRKLAIERAKPYHLSTSSVFRLVSRPKPLSAVPKQVVTGTVLTRSVFINNVLSKIGEVWASKEPINSTTPFNSSKIEEPSPIVYSLPAPELPEAFFGTPISKASQKISRLLDLVVQPVPRPSQCSEFIQQSSMKSPLYLVSRSLPSSSQLKGSPQAISRASELHLLETPLVVKKAKSLAMSVTTSGFSEFTPQSILRSTLRSTPLASPSPSPGRSPQRLKETRISFVEEDVHPKWIPGAADDSKLEVFTTPKKCAVPVETEWLKSKDRTTSFFLNSPEKEHQEMDEGSQSLEKLDVSKGNSSVSITSDETTLEYQDAPSPEDLEETVFTASKPKSSSTALTTNVTEQTEKDGDKDVFASEVTPSDLQKQMGNLEDAETKDLLVAAEAFSELNHLSPVQGTEASLCAPSVYEGKIFTQKSKVPVLDEGLTSVETYTPAIRANDNKSMADVLGDGGNSSLTISEGPIVSERRLNQEVALNLKEDHEVEVGVLKESVDLPEEKLPISDSPPDTQEIHVIEQEKLEAQDSGEEARNLSFNELYPSGTLKLQYNFDTIDQQFCDLADNKDTAECDIAEVDGELFVAQSNFTLILEGEEGEVEPGDFASSDVLPKAANTATEEKLVCSGENDNHGQIANLPSAVTSDQKSQKVDTLPYVPEPIKVAIAENLLDVIKDTRSKEITSDTMEQSIHETIPLVSQNIMCPTKLVKSAFKTAQETSTMTMNVSQVDDVVSSKTRTRGQRIQNVNVKSAQQEASADVATPKMPGQSVRKKTRKAKEISEASENIYSDVRGLSQNQQIPQNSVTPRRGRRKKEVNQDILENTSSVEQELQITTGRESKRLKSSQLLEPAVEETTKKEVKVSSVTKRTPRRIKRSVENQESVEIINDLKVSTVTSPSRMIRKLRSTNLDASENTGNKQDDKSSDKQLRIKHVRRVRGREVSPSDVREDSNLESSQLTVQAEFDMSAIPRKRGRPRKINPSEDVGSKAVKEERSPKKKEAPSIRRRSTRNTPAKSENVDVGKPALGKSILVPNEELSMVMSSKKKLTKKTESQSQKRSLHSVSEERTDEMTHKETNEQEERLLATASFTKSSRSSRTRSSKAILLPDLSEPNNEPLFSPASEVPRKAKAKKIEVPAQLKELVSDLSSQFVISPPALRSRQKNTSNKNKLEDELKDDAQSVETLGKPKAKRIRTSKTKQASKNTEKESAWSPPPIEIRLISPLASPADGVKSKPRKTTEVTGTGLGRNRKKLSSYPKQILRRKML 465452FD42ACCFAE po5f1_mouse POU domain, class 5, transcription factor 1 Pou5f1 Oct-3 Oct-4 Otf-3 Otf3 Octamer-binding transcription factor 3 NF-A3 Octamer-binding protein 3 Octamer-binding protein 4 protein protein mouse Mus musculus Mouse MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPPGGPGIGPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQARKRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRSSIEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSVPVTALGSPMHSN DIP protein P20263 original sequence version: 87 DIP protein P20263 original sequence version: 121 DIP protein P20263 original sequence version: 118 DIP protein P20263 original sequence version: 137 DIP protein P20263 original sequence version: 107 757E41DF52286714 nanog_mouse Homeobox protein NANOG Nanog Ecat4 Enk Homeobox transcription factor Nanog Early embryo specific expression NK-type homeobox protein ES cell-associated protein 4 protein protein mouse Mus musculus Mouse MSVGLPGPHSLPSSEEASNSGNASSMPAVFHPENYSCLQGSATEMLCTEAASPRPSSEDLPLQGSPDSSTSPKQKLSSPEADKGPEEEENKVLARKQKMRTVFSQAQLCALKDRFQKQKYLSLQQMQELSSILNLSYKQVKTWFQNQRMKCKRWQKNQWLKTSNGLIQKGSAPVEYPSIHCSYPQGYLVNASGSLSMWGSQTWTNPTWSSQTWTNPTWNNQTWTNPTWSSQAWTAQSWNGQPWNAAPLHNFGEDFLQPYVQLQQNFSASDLEVNLEATRESHAHFSTPQALELFLNYSVTPPGEI DIP protein Q80Z64 original sequence version: 43 DIP protein Q80Z64 original sequence version: 64 DIP protein Q80Z64 original sequence version: 77 DIP protein Q80Z64 original sequence version: 78 4EF96408B767C79F tif1b_mouse Transcription intermediary factor 1-beta Trim28 Krip1 Tif1b Tripartite motif-containing protein 28 KRAB-A-interacting protein KRIP-1 E3 SUMO-protein ligase TRIM28 Kap1 RING-type E3 ubiquitin transferase TIF1-beta protein protein mouse Mus musculus Mouse MAASAAATAAASAATAASAASGSPGSGEGSAGGEKRPAASSAAAASAAASSPAGGGGEAQELLEHCGVCRERLRPERDPRLLPCLHSACSACLGPATPAAANNSGDGGSAGDGAMVDCPVCKQQCYSKDIVENYFMRDSGSKASSDSQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKTRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKNTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPGPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSDDPYSSAEPHVSGMKRSRSGEGEVSGLLRKVPRVSLERLDLDLTSDSQPPVFKVFPGSTTEDYNLIVIERGAAAAAAGQAGTVPPGAPGAPPLPGMAIVKEEETEAAIGAPPAAPEGPETKPVLMPLTEGPGAEGPRLASPSGSTSSGLEVVAPEVTSAPVSGPGILDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDSTFSMEQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNDAFGDTKFSAVLVEPPPLNLPSAGLSSQELSGPGDGP DIP protein Q62318 original sequence version: 129 DIP protein Q62318 original sequence version: 120 DIP protein Q62318 original sequence version: 138 DIP protein Q62318 original sequence version: 152 DIP protein Q62318 original sequence version: 166 DIP protein Q62318 original sequence version: 139 DE87AAA5DC67BB8B hdac2_mouse Histone deacetylase 2 Hdac2 Yy1bp YY1 transcription factor-binding protein protein protein mouse Mus musculus Mouse MAYSQGGGKKKVCYYYDGDIGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKATAEEMTKYHSDEYIKFLRSIRPDNMSEYSKQMQRFNVGEDCPVFDGLFEFCQLSTGGSVAGAVKLNRQQTDMAVNWAGGLHHAKKSEASGFCYVNDIVLAILELLKYHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNFPMRDGIDDESYGQIFKPIISKVMEMYQPSAVVLQCGADSLSGDRLGCFNLTVKGHAKCVEVAKTFNLPLLMLGGGGYTIRNVARCWTYETAVALDCEIPNELPYNDYFEYFGPDFKLHISPSNMTNQNTPEYMEKIKQRLFENLRMLPHAPGVQMQAIPEDAVHEDSGDEDGEDPDKRISIRASDKRIACDEEFSDSEDEGEGGRRNVADHKKGAKKARIEEDKKETEDKKTDVKEEDKSKDNSGEKTDPKGAKSEQLSNP DIP protein P70288 original sequence version: 83 DIP protein P70288 original sequence version: 123 DIP protein P70288 original sequence version: 112 DIP protein P70288 original sequence version: 99 DIP protein P70288 original sequence version: 155 DIP protein P70288 original sequence version: 117 B9843D24A775157C zfp42_mouse Zinc finger protein 42 Zfp42 Rex-1 Rex1 Reduced expression protein 1 protein protein mouse Mus musculus Mouse MNEQKMNEQMKKTAKTSGQKGPGGRALDRLTLKQDEARPVQNTRVEAPRVTYTIRDESEISPETEEDGFPDGYLECIIRGEFSEPILEEDFLFKSFESLEEVEQNLSRQVLEASSLLESSLEYMTKGTKQEKTEVTQETPPLRVGASSLLAGGPAEKPEGGVYCGVLSMLECPQAGCKKKLRGKTALRKHMLVHGPRRHVCAECGKAFTESSKLKRHFLVHTGEKPYQCTFEGCGKRFSLDFNLRTHIRIHTGERRFVCPFDGCEKSFIQSNNQKIHILTHAKAGKKC DIP protein P22227 original sequence version: 105 4B1ABFDF372A7D7B rest_mouse RE1-silencing transcription factor Rest Nrsf Neural-restrictive silencer factor protein protein mouse Mus musculus Mouse MATQVMGQSSGGGSLFNNSANMGMALTNDMYDLHELSKAELAAPQLIMLANVALTGEASGSCCDYLVGEERQMAELMPVGDNHFSESEGEGLEESADLKGLENMELGSLELSAVEPQPVFEASAAPEIYSANKDPAPETPVAEDKCRSSKAKPFRCKPCQYEAESEEQFVHHIRIHSAKKFFVEESAEKQAKAWESGSSPAEEGEFSKGPIRCDRCGYNTNRYDHYMAHLKHHLRAGENERIYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCSKCNYFSDRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCNYVASNQHEVTRHARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPSKTMDVSKVKLKKTKKREADLLNNAVSNEKMENEQTKTKGDVSGKKNEKPVKAVGKDASKEKKPGSSVSVVQVTTRTRKSAVAAETKAAEVKHTDGQTGNNPEKPCKAKKNKRKKDAEAHPSEEPVNEGPVTKKKKKSECKSKIGTNVPKGGGRAEERPGVKKQSASLKKGTKKTPPKTKTSKKGGKLAPKGMGQTEPSSGALAQVGVSPDPALIQAEVTGSGSSQTELPSPMDIAKSEPAQMEVSLTGPPPVEPAQMEPSPAKPPQVEAPTYPQPPQRGPAPPTGPAPPTGPAPPTEPAPPTGLAEMEPSPTEPSQKEPPPSMEPPCPEELPQAEPPPMEDCQKELPSPVEPAQIEVAQTAPTQVQEEPPPVSEPPRVKPTKRSSLRKDRAEKELSLLSEMARQEQVLMGVGLVPVRDSKLLKGNKSAQDPPAPPSPSPKGNSREETPKDQEMVSDGEGTIVFPLKKGGPEEAGESPAELAALKESARVSSSEQNSAMPEGGASHSKCQTGSSGLCDVDTEQKTDTVPMKDSAAEPVSPPTPTVDRDAGSPAVVASPPITLAENESQEIDEDEGIHSHDGSDLSDNMSEGSDDSGLHGARPTPPEATSKNGKAGLAGKVTEGEFVCIFCDRSFRKEKDYSKHLNRHLVNVYFLEEAAEEQEEQEEREEQE 0D3616BC0820BC2F sall1_mouse Sal-like protein 1 Sall1 Sal3 Zinc finger protein Spalt-3 protein protein mouse Mus musculus Mouse MSRRKQAKPQHFQSDPEVASLPRRDGDTEKGQPSRPTKSKDAHVCGRCCAEFFELSDLLLHKKSCTKNQLVLIVNESPASPAKTFPPGPSLNDPDDQMKDAANKADQEDCSDLSEPKGLDREESMEVEVPVATTTTTTTGGSGGSGGSTLSGVTNITTPSCHSGCSSGTSAITTSLPQLGDLTTLGNFSVINSNVIIENLQSTKVAVAQFSQEARCGGASGGKLLISTLMEQLLALQQQQIHQLQLIEQIRHQILLLASQSADLPAAPSIPSQGTLRTSANPLTTLSSHLSQQLAVAAGLAQSLASQSANISGVKQLPHVQLPQSSSGTSIVPPSGGTSPNMSIVTAAVPTPSSEKVASNAGASHVSSPAVSASSSPAFAISSLLSPESNPLLPQPTPANAVFPTPLPNIATTAEDLNSLSALAQQRKSKPPNVTAFEAKSTSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKEKYPHIQMNPYPVPEHLDNVPTSTGIPYGMSIPSEKPVTSWLDTKPVLPTLTTSVGLPLPPTLPSLTPFIKTEEPAPIPISHSAASPQGSVKSDSGAPDLATRNPSGVPEEVEGSAVPPFGGKGEESNMASSAVPTAGNSTLNSPVADGGPGGTTFTNPLLPLMSEQFKAKFPFGGLLDSAQASETSKLQQLVENIDKKATDPNECIICHRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPICQKKFTNAVVLQQHIRMHMGGQIPNTPVPDNYPESMESDTGSFDEKNFDDLDNFSDENMEECPEGSIPDTPKSADASQDSLSSSPLPLEMSSIAALENQMKMINAGLAEQLQASLKSVENGSMEGDVLTNDSSSVGGDMESQSAGSPAISESTSSMQALSPSNSTQEFHKSPGMEEKPQRVGPGEFANGLSPTPVNGGALDLTSSHAEKIIKEDSLGILFPFRDRGKFKNTACDICGKTFACQSALDIHYRSHTKERPFICTVCNRGFSTKGNLKQHMLTHQMRDLPSQLFEPSSNLGPNQNSAVIPANSLSSLIKTEVNGFVHVSPQDSKDAPTSHVPQGPLSSSATSPVLLPALPRRTPKQHYCNTCGKTFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPARRGRRLSVDGPMTFLGGNPVKFPEMFQKDLAARSGSGDPSSFWNQYTAALSNGLAMKANEISVIQNGGIPPIPGSLGSGSSPISGLTGNVEKLGNSEPSAPLAGLEKMASSENGTNFRFTRFVEDSKEIVTS DIP protein Q9ER74 original sequence version: 80 DF4FEF7FEA0B9F5C nac1_mouse Sodium/calcium exchanger 1 Slc8a1 Ncx Na(+)/Ca(2+)-exchange protein 1 Solute carrier family 8 member 1 protein protein mouse Mus musculus Mouse MLRLSLPPNVSMGFRLVALVALLFSHVDHITADTEAETGGNETTECTGSYYCKKGVILPIWEPQDPSFGDKIARATVYFVAMVYMFLGVSIIADRFMSSIEVITSQEKEITIKKPNGETTKTTVRIWNETVSNLTLMALGSSAPEILLSVIEVCGHNFTAGDLGPSTIVGSAAFNMFIIIALCVYVVPDGETRKIKHLRVFFVTAAWSIFAYTWLYIILSVSSPGVVEVWEGLLTFFFFPICVVFAWVADRRLLFYKYVYKRYRAGKQRGMIIEHEGDRPASKTEIEMDGKVVNSHVDNFLDGALVLEVDERDQDDEEARREMARILKELKQKHPEKEIEQLIELANYQVLSQQQKSRAFYRIQATRLMTGAGNILKRHAADQARKAVSMHEVNMEMAENDPVSKIFFEQGTYQCLENCGTVALTIMRRGGDLSTTVFVDFRTEDGTANAASDYEFTEGTVIFKPGETQKEIRVGIIDDDIFEEDENFLVHLSNVRVSSDVSEDGILESNHASSIACLGSPSTATITIFDDDHAGIFTFEEPVTHVSESIGIMEVKVLRTSGARGNVIIPYKTIEGTARGGGEDFEDTCGEPEFQNDEIVKTISVKVIDDEEYEKNKTFFIEIGEPRLVEMSEKKALLLNELGGFTLTGKEMYGQPIFRKVHARDHPIPSTVITISEEYDDKQPLTSKEEEERRIAEMGRPILGEHTKLEVIIQESYEFKSTVDKLIKKTNLALVVGTNSWREQFIEAITVSAGEDDDDDECGEEKLPSCFDYVMHFLTVFWKVLFAFVPPTEYWNGWACFIVSILMIGLLTAFIGDLASHFGCTIGLKDSVTAVVFVALGTSVPDTFASKVAATQDQYADASIGNVTGSNAVNVFLGIGVAWSIAAIYHAANGEQFKVSPGTLAFSVTLFTIFAFINVGVLLYRRRPEIGGELGGPRTAKLLTSSLFVLLWLLYIFFSSLEAYCHIKGF F5FC0BD07F2B6602 rif1_mouse Telomere-associated protein RIF1 Rif1 Rap1-interacting factor 1 homolog protein protein mouse Mus musculus Mouse MTAPGRSPLEPLLETWEDPSVPPGEQTDAYLTLTSRMTGEEGKEVIAEIEKNLSRLYTVLKAHISSQNSELSSAALQALGFCLYNPRITSGLSEANIQELLLTLNGIIKSSDKNVCTRALWVISKQTFPAELVSKMVSSIIDSLEVILSKGEIHSAVVDFEALNVIIRLIEQAPVQMGEESVRWAKLVIPLVVHSAQKVHLRGATALEMGMPLLLQKQQEIALITEHLMTTKLISELQKLFKNKNETYVLKLWPLFVKLLGKTLHRSGSFINSLLQLEELGFRSGTPMIKKIAFIAWKSLIDNFALNPDILCSAKRLKLLMQPLSSIHVRTETLALTKLEVWWYLLMRLGPQLPANFEQVCVPLIQSTISVDSIPSPQGNSSRGSASPGLSPLTPGHKGASPYGSPRGNLSSNTGGMAAIPSIQLLGLEMMLHFLLGPEVLSFAKQHKIVLSLEPLEHPLISSPSFFSKYAHTLITAVHDSFVSVGKDASDAVVSAIWKELISLVKSVTEAGNRKEKSGSEVLTLLLKSLENIVKSEVFPVSKTLVLMEITVKGLPPKVLGSPAYQVANMDILNGTPALFLIQLIFNNNLLECGVEDEKFFLNLETLVGCVLSGPTSPLAFSDSVLTVINQNAKQLVNKEHLWRMWSMIVSPLTDVIHQTNEVNQGDALEHNFSAIYGALTLPINHIFSAQTFPTGTMKALLKTWSELYRAFTRCASLVATAEENLCCEELSSKIMCSLEDEVLSDLLFLDRISHIIIVMVDCIDFSPYNKKYQPKIKSPQRSSDWSRKKKEPLGKLASLFKLIVKVIDTFHTLSLKETFSDTLLAIGNSIISMLSNVFGHISLPSMIREIFATFTRPLALLYENSKLDEAPKVYTSLNNKLEKLLGEIVACLQFSYLGAYDSELLEHLSPLLCVIFLHKNKQIRKQSALLWNATFAKATALVYPEELKPILRQAKQKILLLLPGLENVEMMDESSEPYSESTENSQLNVKISGMERKSSGKRDSILAHTKDKKKKVKLSAKLKLESSSPKIKSGKLLEEEKSTDFVFIPPEGKETKARVLTEHQKEVLKTKRCDIPALYNNLDASQDTLFSAQFSQEESMESLTLTEKPKEDAKIIKEEQMESTIFIHQDAPENCGIDEHSENASLPNCGGSVAETNPETLITGFDARKEVLISSKILSAESSSSTETSVVSSSSVSNATFSGTPPQPTSRRQTFITLEKFDGSETRPFSPSPLNNISSTVTVRNNQDNTTNTDMPPKARKREVTNSKSDSENLANAGKKSSRRWSKAEQSVTKKSKPSLTSEQEEHSSENNSPDLLSPTEHVSENDDHPSEATLEHKDGDPKPAVENASLEDLTTEEKNVGINMESKESTASVVARTEQIVNEDSQAAALAPNPKTLRRSSRRRSEAVDSCSDSQERESGQQKKERRKEEEKIISKSPLRIKDDKLPTQKLTDESPIQENLTEKGNTLPERTSGEPSVNAEIDQNRRKPDLENVSSEGGGGTLDNLDKSSEKPLRGRTRYQTRRASQGLISAVENSESDSSEAKEEVSRKKRSGKWKNRSSDSVDIEEQEEKKAEEEVMKTANQTLDGQAVPDVDVNAAAQVCEKSTNNNRVILQDSAGPADSLQAPPKGEEKSKINKCVDSSFVSLPVPESNLRTRNASKRLLYKQDNDSNVRVSDSSLSPEKFTQVECQHKRSRRVRRSKSCDCCGEKSQSQEKSFIGLKNTESYAIKSVEKKKTDLQVPETAPETREARDHAETKLAGEEPLVNFHVGLKEENCTTGDSVKSEAELQEASLPPEIVTVKEKTYDTDASEAVSEIQGPCSENHSPAEDPGLSECKDISQKQLSENGELDISDVGKACKVIAGSSPEGVETMELNVRNDAFVAADSEKSTQMDVSVDVATEEDNKKDECEAVTTEVNVEGVATEDFNSGMDLSDTPIPVSKDVETEHAASGEIEGESNESDSGSCEEMNKEMGSHKAQMSTEIDSARVKETDILASASKSEEALIGRLDVNTQSFVSDIEMSSGERTVNCKTETSIELNKLDEAKLSGNEATVGNDTLQEVCFTSEKVEKLPQCLLVQVASELGAESNTTSPEKLELDSFGSVNESPSGMQQARCVWSPLASPSTSILKRGLKRSQEDEISPVNKIRRVSFADPIYQAGLADDIDRRCSVVRSHSSNSSPIIKSVKTSPTSHSKHNTTSAKGFLSPGSQSSKFKSPKKCLITEMAQESMLSPTESVYPALVNCAASVDIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVFNVKKALRVYHEQQMKSRGLEEIPIFDISEKAVNGVESRTVSTDEERFASDLIEPVTLDTPLSKNLVAQISALALQLDSEDLYSYTGSQLFEMHEKLGTMANSIIRNLQSRWRSPAHENS DIP protein Q6PR54 original sequence version: 54 016A1A4CEF25B8F9 nr0b1_mouse Nuclear receptor subfamily 0 group B member 1 Nr0b1 Ahch Dax1 Nuclear receptor DAX-1 protein protein mouse Mus musculus Mouse MAGEDHPWQGSILYNLLMSAKQKHASQEEREVRLGAQCWGCACGAQPVLGGERLSGGQARSLLYRCCFCGENHPRQGGILYSMLTNARQPSVATQAPRARFGAPCWGCACGSAEPLVGREGLPAGQAPSLLYRCCFCGEEHPRQGSILYSLLTSAQQTHVSREAPEAHRRGEWWQLSYCTQSVGGPEGLQSTQAMAFLYRSYVCGEEQPQQISVASGTPVSADQTPATPQEQPRAPWWDASPGVQRLITLKDPQVVCEAASAGLLKTLRFVKYLPCFQILPLDQQLVLVRSCWAPLLMLELAQDHLHFEMMEIPETNTTQEMLTTRRQETEGPEPAEPQATEQPQMVSAEAGHLLPAAAVQAIKSFFFKCWSLNIDTKEYAYLKGTVLFNPDLPGLQCVKYIEGLQWRTQQILTEHIRMMQREYQIRSAELNSALFLLRFINSDVVTELFFRPIIGAVSMDDMMLEMLCAKL DIP protein Q61066 original sequence version: 72 DE1FB01C30F883CF nanog-rif1 3 20 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 26 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 25 unspecified role unspecified role prey prey 27 unspecified role unspecified role prey prey association association 1D The first group includes proteins present in at least two of three independent one-step purifications and tandem purification. These proteins (Sall1, Sall4, Rif1, Tif1, Mybbp, Dax1 and Nac1) are prime interaction candidates. The second group includes proteins (Zfp281, Err2, Elys, Oct4, Zfp198, NF45 and HDAC2) that may be part of unstable or transient complexes that dissociate during tandem purification. Proteins in the third group (REST, Sp1 and Wapl) seem 'masked' in one-step purifications but are recovered in the tandem procedure. dax1-nanog 7 27 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 19 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2a nac1-nanog-1 7 25 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 19 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2b zfp281-nanog-2 7 19 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2c oct4-nanog 7 19 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 18 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2d nanog-rif1-2 5 19 unspecified role unspecified role prey prey 26 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2g Furthermore, we confirmed the association of Rif1 with endogenous Nanog (Fig. 2g), as well as ectopically expressed Nanog (Fig. 2h, i). dax1-nanog-1 4 19 unspecified role unspecified role prey prey 27 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2a Interactions between Nanog and Dax1, Nac1, Zfp281 and Oct4 were confirmed in heterologous 293T (Supplementary Fig. S2) and ES (Fig. 2a d) cells transiently transfected with constructs as indicated nac1-nanog-2 4 27 unspecified role unspecified role prey prey 25 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2b Interactions between Nanog and Dax1, Nac1, Zfp281 and Oct4 were confirmed in heterologous 293T (Supplementary Fig. S2) and ES (Fig. 2a d) cells transiently transfected with constructs as indicated zfp281-nanog-3 4 15 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 27 unspecified role unspecified role prey prey physical association physical association 2c Interactions between Nanog and Dax1, Nac1, Zfp281 and Oct4 were confirmed in heterologous 293T (Supplementary Fig. S2) and ES (Fig. 2a d) cells transiently transfected with constructs as indicated oct4-nanog-1 4 18 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 27 unspecified role unspecified role prey prey physical association physical association 2d Interactions between Nanog and Dax1, Nac1, Zfp281 and Oct4 were confirmed in heterologous 293T (Supplementary Fig. S2) and ES (Fig. 2a d) cells transiently transfected with constructs as indicated nanog-rif1-1 4 26 unspecified role unspecified role prey prey 27 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2h Furthermore, we confirmed the association of Rif1 with endogenous Nanog (Fig. 2g), as well as ectopically expressed Nanog (Fig. 2h, i). nanog-rif1-3 5 19 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 26 unspecified role unspecified role prey prey physical association physical association 2g Furthermore, we confirmed the association of Rif1 with endogenous Nanog (Fig. 2g), as well as ectopically expressed Nanog (Fig. 2h, i). nac1-nanog-3 2 25 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2f We confirmed that Nanog co-purifies with bioNac1, bioZfp281, bioDax1 and bioOct4 (Fig. 2e) and that Nac1 co-purifies with bioNanog (Fig. 2f). rif1-nanog 2 19 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 26 unspecified role unspecified role prey prey physical association physical association 2i Furthermore, we confirmed the association of Rif1 with endogenous Nanog (Fig. 2g), as well as ectopically expressed Nanog (Fig. 2h, i). nac1-nanog-4 2 19 unspecified role unspecified role prey prey 25 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2e We confirmed that Nanog co-purifies with bioNac1, bioZfp281, bioDax1 and bioOct4 (Fig. 2e) and that Nac1 co-purifies with bioNanog (Fig. 2f). zfp281-nanog-4 2 15 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 19 unspecified role unspecified role prey prey physical association physical association 2e We confirmed that Nanog co-purifies with bioNac1, bioZfp281, bioDax1 and bioOct4 (Fig. 2e) and that Nac1 co-purifies with bioNanog (Fig. 2f). dax1-nanog-2 2 27 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 19 unspecified role unspecified role prey prey physical association physical association 2e We confirmed that Nanog co-purifies with bioNac1, bioZfp281, bioDax1 and bioOct4 (Fig. 2e) and that Nac1 co-purifies with bioNanog (Fig. 2f). oct4-nanog-2 2 19 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2e We confirmed that Nanog co-purifies with bioNac1, bioZfp281, bioDax1 and bioOct4 (Fig. 2e) and that Nac1 co-purifies with bioNanog (Fig. 2f). dax1-nanog-3 1 19 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 27 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2e nac1-nanog-5 1 19 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 25 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2f zfp281-nanog-5 1 15 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 19 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2g oct4-nanog-3 1 19 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 18 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2h nanog-nac1 8 22 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 19 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 4MD complex. S7 The size distributions of complexes containing Oct4, Dax1, Nac1, Zfp281 and Rex1 overlap that of Nanog (Supplementary Fig. S7). nanog-dax1 8 19 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 27 unspecified role unspecified role neutral component neutral component association association 700-440kD complex. S7 The size distributions of complexes containing Oct4, Dax1, Nac1, Zfp281 and Rex1 overlap that of Nanog (Supplementary Fig. S7). nanog-oct4 8 22 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 25 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 18 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component association association 232-158 kD complex. S7 The size distributions of complexes containing Oct4, Dax1, Nac1, Zfp281 and Rex1 overlap that of Nanog (Supplementary Fig. S7). nanog-rif1-4 6 14 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 26 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 25 unspecified role unspecified role prey prey 27 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 1D The first group includes proteins present in at least two of three independent one-step purifications and tandem purification. These proteins (Sall1, Sall4, Rif1, Tif1, Mybbp, Dax1 and Nac1) are prime interaction candidates. The second group includes proteins (Zfp281, Err2, Elys, Oct4, Zfp198, NF45 and HDAC2) that may be part of unstable or transient complexes that dissociate during tandem purification. Proteins in the third group (REST, Sp1 and Wapl) seem 'masked' in one-step purifications but are recovered in the tandem procedure.