IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Arabidopsis pex12 and pex13 mutants are defective in both PTS1- and PTS2-dependent protein transport to peroxisomes. The Arabidopsis pex12 and pex13 mutants are defective in both PTS1- and PTS2-dependent protein transport to peroxisomes. Plant J. (0960-7412) 2006 imex curation Mano S., Nakamori C., Nito K., Kondo M., Nishimura M. mikosome@nibb.ac.jp 01-Sep-2009: Contacted by JYOTI. jyoti Only protein-protein interactions arath-root Arabidopsis thaliana root tissue root confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Mano S., Nakamori C., Nito K., Kondo M., Nishimura M. mikosome@nibb.ac.jp Accepted 2009-SEP-18 by JYOTI. 01-Sep-2009: Contacted by JYOTI. Plant J. (0960-7412) 2006 jyoti Only protein-protein interactions imex curation The Arabidopsis pex12 and pex13 mutants are defective in both PTS1- and PTS2-dependent protein transport to peroxisomes. The Arabidopsis pex12 and pex13 mutants are defective in both PTS1- and PTS2-dependent protein transport to peroxisomes. Plant J. (0960-7412) 2006 imex curation Mano S., Nakamori C., Nito K., Kondo M., Nishimura M. mikosome@nibb.ac.jp 01-Sep-2009: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Mano S., Nakamori C., Nito K., Kondo M., Nishimura M. mikosome@nibb.ac.jp Accepted 2009-SEP-18 by JYOTI. 01-Sep-2009: Contacted by JYOTI. Plant J. (0960-7412) 2006 jyoti Only protein-protein interactions imex curation pex12_arath Peroxisome biogenesis protein 12 At3g04460 T27C4.11 Peroxin-12 PEX12 APM4 Pex12p Protein ABERRANT PEROXISOME MORPHOLOGY 4 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLFQVGGEGTRPTFFEMAAAQQLPASLRAALTYSLGVFALRRSFLHKILDYEDEFFAALMLILEGHSLRTTDGSFAESLYGLRRKSARLRLRKDSARKDSSEEVQHSGLEKRQRILSVVFLVVLPYFKSKLHAIYNKEREARLRESLWGAEDQGFDEADFFTGDDSIVSREPSGNEELSVRVQLATKIKKFIAVCYPWIHASSEGLSFTYQLLYLLDATGFYSLGLQALGIQVCRATGQELMDTSSRISKIRNHERERLRGPPWLKTVQGALLSCSYAVLDYAQTGLIAAVFIFKMMEWWYQSAEERLSAPTVYPPPPPPPAPKMAKEGIPLPPDRSLCALCLQKRANPSVVTVSGFVFCYSCVFKYVSKYKRCPVTLIPASVDQIRRLFQDT BEF9C580A93F52A7 pex13_arath Peroxisomal membrane protein 13 PEX13 APM2 Peroxin-13 Peroxisome biogenesis protein 13 Pex13p Protein ABERRANT PEROXISOME MORPHOLOGY 2 ABSTINENCE BY MUTUAL CONSENT AMC Protein AMC F21O3.27 At3g07560 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASQPAGGSPPKPWEKEGNTSGPNPFRPPSNTSTAGSVEASGTANPGEVVPPPVNRPNTAANMNSLSRPVPARPWEQQNYGSTMGGGYGSNLGMTSGYGSGTYGSALGGYGSSYGGGMYGGSSMYRGGYGGGGLYGSSGMYGGGAMGGYGGTMGGYGMGMGTGMGMGMGMGMGGPYGSQDPNDPFNQPPSPPGFWISFLRVMQGAVNFFGRVAMLIDQNTQAFHMFMSALLQLFDRGGMLYGELARFVLRMLGVRTRPRKMQQPPQGPNGLPLPHQPHGNQNYLEGPKTAAPGGGGGWDNVWGN 246722710D2D3D6C hpr1_arath Glycerate dehydrogenase HPR, peroxisomal HPR NADH-dependent hydroxypyruvate reductase 1 T23K23.14 At1g68010 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAKPVSIEVYNPNGKYRVVSTKPMPGTRWINLLVDQGCRVEICHLKKTILSVEDIIDLIGDKCDGVIGQLTEDWGETLFSALSKAGGKAFSNMAVGYNNVDVEAANKYGIAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRGGLYEGWLPHLFVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGQFLKANGEQPVTWKRASSMEEVLREADLISLHPVLDKTTYHLVNKERLAMMKKEAILVNCSRGPVIDEAALVEHLKENPMFRVGLDVFEEEPFMKPGLADTKNAIVVPHIASASKWTREGMATLAALNVLGRVKGYPIWHDPNRVDPFLNENASPPNASPSIVNSKALGLPVSKL CF900B10C44055BD pex12-hpr-2 1 3 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization Figure 7c When GFP-PTS1 and RFP-PTS1 were expressed simultaneously as a control, peroxisomal matrix was labelled with both GFP (Figure 7a) and RFP pex13-hpr-3 1 4 unspecified role unspecified role neutral component neutral component n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization Figure 7i pex13-hpr-4 2 4 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey n-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 1 PEX7 interacted with PEX13N2 but did not interact with PEX13C (Table 1). These data indicate that the amino-terminal 100 amino acids of PEX13 are capable of binding with PEX7.