IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation. Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation. J. Cell Biol. (0021-9525) 2005 imex curation Holzel M., Rohrmoser M., Schlee M., Grimm T., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Hiddemann W., Bornkamm GW., Eick D. eick@gsf.de 23-Feb-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Holzel M., Rohrmoser M., Schlee M., Grimm T., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Hiddemann W., Bornkamm GW., Eick D. eick@gsf.de Accepted 2009-Not a month!-22 by JYOTI. 23-Feb-2010: Contacted by JYOTI. J. Cell Biol. (0021-9525) 2005 jyoti Only protein-protein interactions imex curation Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation. Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation. J. Cell Biol. (0021-9525) 2005 imex curation Holzel M., Rohrmoser M., Schlee M., Grimm T., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Hiddemann W., Bornkamm GW., Eick D. eick@gsf.de 23-Feb-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel western blot western blot Immuno blot Immuno blot Holzel M., Rohrmoser M., Schlee M., Grimm T., Harasim T., Malamoussi A., Gruber-Eber A., Kremmer E., Hiddemann W., Bornkamm GW., Eick D. Total cell lysates of U2OS cells were separated by native gel electrophoresis, followed by a second denaturing gel electrophoresis. eick@gsf.de Accepted 2009-Not a month!-22 by JYOTI. 23-Feb-2010: Contacted by JYOTI. J. Cell Biol. (0021-9525) 2005 jyoti Only protein-protein interactions imex curation bop1_human Ribosome biogenesis protein BOP1 Block of proliferation 1 protein BOP1 KIAA0124 protein protein human Homo sapiens Human MAGSRGAGRTAAPSVRPEKRRSEPELEPEPEPEPPLLCTSPLSHSTGSDSGVSDSEESVFSGLEDSGSDSSEDDDEGDEEGEDGALDDEGHSGIKKTTEEQVQASTPCPRTEMASARIGDEYAEDSSDEEDIRNTVGNVPLEWYDDFPHVGYDLDGRRIYKPLRTRDELDQFLDKMDDPDYWRTVQDPMTGRDLRLTDEQVALVRRLQSGQFGDVGFNPYEPAVDFFSGDVMIHPVTNRPADKRSFIPSLVEKEKVSRMVHAIKMGWIQPRRPRDPTPSFYDLWAQEDPNAVLGRHKMHVPAPKLALPGHAESYNPPPEYLLSEEERLAWEQQEPGERKLSFLPRKFPSLRAVPAYGRFIQERFERCLDLYLCPRQRKMRVNVDPEDLIPKLPRPRDLQPFPTCQALVYRGHSDLVRCLSVSPGGQWLVSGSDDGSLRLWEVATARCVRTVPVGGVVKSVAWNPSPAVCLVAAAVEDSVLLLNPALGDRLVAGSTDQLLSAFVPPEEPPLQPARWLEASEEERQVGLRLRICHGKPVTQVTWHGRGDYLAVVLATQGHTQVLIHQLSRRRSQSPFRRSHGQVQRVAFHPARPFLLVASQRSVRLYHLLRQELTKKLMPNCKWVSSLAVHPAGDNVICGSYDSKLVWFDLDLSTKPYRMLRHHKKALRAVAFHPRYPLFASGSDDGSVIVCHGMVYNDLLQNPLLVPVKVLKGHVLTRDLGVLDVIFHPTQPWVFSSGADGTVRLFT 49A97BE21B0EB3DD wdr12_human Ribosome biogenesis protein WDR12 WDR12 WD repeat-containing protein 12 protein protein human Homo sapiens Human MAQLQTRFYTDNKKYAVDDVPFSIPAASEIADLSNIINKLLKDKNEFHKHVEFDFLIKGQFLRMPLDKHMEMENISSEEVVEIEYVEKYTAPQPEQCMFHDDWISSIKGAEEWILTGSYDKTSRIWSLEGKSIMTIVGHTDVVKDVAWVKKDSLSCLLLSASMDQTILLWEWNVERNKVKALHCCRGHAGSVDSIAVDGSGTKFCSGSWDKMLKIWSTVPTDEEDEMEESTNRPRKKQKTEQLGLTRTPIVTLSGHMEAVSSVLWSDAEEICSASWDHTIRVWDVESGSLKSTLTGNKVFNCISYSPLCKRLASGSTDRHIRLWDPRTKDGSLVSLSLTSHTGWVTSVKWSPTHEQQLISGSLDNIVKLWDTRSCKAPLYDLAAHEDKVLSVDWTDTGLLLSGGADNKLYSYRYSPTTSHVGA 6D088C640AC981D8 pesc_human Pescadillo homolog PES1 protein protein human Homo sapiens Human MGGLEKKKYERGSATNYITRNKARKKLQLSLADFRRLCILKGIYPHEPKHKKKVNKGSTAARTFYLIKDIRFLLHEPIVNKFREYKVFVRKLRKAYGKSEWNTVERLKDNKPNYKLDHIIKERYPTFIDALRDLDDALSMCFLFSTFPRTGKCHVQTIQLCRRLTVEFMHYIIAARALRKVFLSIKGIYYQAEVLGQPIVWITPYAFSHDHPTDVDYRVMATFTEFYTTLLGFVNFRLYQLLNLHYPPKLEGQAQAEAKAGEGTYALDSESCMEKLAALSASLARVVVPATEEEAEVDEFPTDGEMSAQEEDRRKELEAQEKHKKLFEGLKFFLNREVPREALAFIIRSFGGEVSWDKSLCIGATYDVTDSRITHQIVDRPGQQTSVIGRCYVQPQWVFDSVNARLLLPVAEYFSGVQLPPHLSPFVTEKEGDYVPPEKLKLLALQRGEDPGNLNESEEEEEEDDNNEGDGDEEGENEEEEEDAEAGSEKEEEARLAALEEQRMEGKKPRVMAGTLKLEDKQRLAQEEESEAKRLAIMMMKKREKYLYQKIMFGKRRKIREANKLAEKRKAHDEAVRSEKKAKKARPE CB6201D6E34D82B1 bop1-pesc-1 1 5 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey association association Endogenous WDR12 forms a stable complex with endogenous Pes1 and Bop1. Fig. 7A wdr12-bop1-1 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait association association Endogenous WDR12 forms a stable complex with endogenous Pes1 and Bop1. Fig. 7A wdr12-pesc-1 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait association association Endogenous WDR12 forms a stable complex with endogenous Pes1 and Bop1. Fig. 7A bop1-pesc-2 1 5 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait association association Endogenous WDR12 forms a stable complex with endogenous Pes1 and Bop1. Fig. 7A wdr12-pesc-2 1 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey association association Endogenous WDR12 forms a stable complex with endogenous Pes1 and Bop1. Fig. 7 wdr12-bop1-2 1 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey association association Endogenous WDR12 forms a stable complex with endogenous Pes1 and Bop1. Fig. 7A wdr12-pesc-3 2 5 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component association association Endogenous WDR12 forms a stable complex with endogenous Pes1 and Bop1. Fig. 7B