IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J. Biol. Chem. (0021-9258) 2004 imex curation Reinke A., Anderson S., McCaffery JM., Yates J., Aronova S., Chu S., Fairclough S., Iverson C., Wedaman KP., Powers T. tpowers@ucdavis.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Reinke A., Anderson S., McCaffery JM., Yates J., Aronova S., Chu S., Fairclough S., Iverson C., Wedaman KP., Powers T. tpowers@ucdavis.edu Accepted 2009-OCT-17 by DAVET. 27-Oct-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2004 davet Only protein-protein interactions imex curation TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae. J. Biol. Chem. (0021-9258) 2004 imex curation Reinke A., Anderson S., McCaffery JM., Yates J., Aronova S., Chu S., Fairclough S., Iverson C., Wedaman KP., Powers T. tpowers@ucdavis.edu 27-Oct-2007: Contacted by JYOTI. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Reinke A., Anderson S., McCaffery JM., Yates J., Aronova S., Chu S., Fairclough S., Iverson C., Wedaman KP., Powers T. tpowers@ucdavis.edu Accepted 2009-OCT-17 by DAVET. J. Biol. Chem. (0021-9258) 2004 davet Only protein-protein interactions imex curation lst8_yeast Target of rapamycin complex subunit LST8 Lethal with SEC13 protein 8 YNL006W N2005 LST8 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSVILVSAGYDHTIRFWEALTGVCSRTIQHSDSQVNRLEITNDKKLLATAGHQNVRLYDIRTTNPNPVASFEGHRGNVTSVSFQQDNRWMVTSSEDGTIKVWDVRSPSIPRNYKHNAPVNEVVIHPNQGELISCDRDGNIRIWDLGENQCTHQLTPEDDTSLQSLSMASDGSMLAAANTKGNCYVWEMPNHTDASHLKPVTKFRAHSTYITRILLSSDVKHLATCSADHTARVWSIDDDFKLETTLDGHQRWVWDCAFSADSAYLVTASSDHYVRLWDLSTREIVRQYGGHHKGAVCVALNDV 536AA1DB8E8151F0 536AA1DB8E8151F0 bit61_yeast Target of rapamycin complex 2 subunit BIT61 YJL058C J1141 61 kDa binding partner of TOR2 protein BIT61 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTAEDILLRERTSTTTQRPVNSEQYLNVQLATAPVKNFQTTSEISRQTLVDTSNDDVYSIKNLKGSRNPISPSVSNVGFQSIFHTVDHPRSKVSVASNHSLRSNDNASAATSKSGSSQIGESHSVDTVECSNNLSKKLSSDAISITQKSLHSTPSGRYMKGKASGFFNRRNRAHTTISSDPASFLTDSSTLHNSSHSFRNVIKNFFQNKSHRHIGQDAIEPAIPNSLSKFLHSSYGRHKSPSQFIHTNAGQLVDSGTSVYSLNVNPSGVNPNTIVEDPLSGTDPASPNPVSMLHDLLRNLPSLEANYKHFNSQELTTLTNNIWNIFCSNVAELFRTQRIWKLRAKIENFNEVLEFYCILKTDPRVTHSGMNRIISDLKEFLVSSLYNLENQIVFNYSNEDTINNALKRLGVIWRIFYQEVYYDLAAVLLPLDQSIREDGNSTVLKSGNESRTHINGNYSIGFLLLMCFRDSIVLPCYENFVNSNDGISKSFQLYIFNQEEESNVTETDKLTLLQCFGILSTIQSNDRNQRIIEELLAGIRMSI 99F0F96840311F4B 99F0F96840311F4B avo2_yeast Target of rapamycin complex 2 subunit AVO2 YMR068W YM9916.07 AVO2 Adheres voraciously to TOR2 protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLKEPSVRLREAIIEGNLLIVKRLLRRNPDLLTNIDSENGWSSLHYASYHGRYLICVYLIQLGHDKHELIKTFKGNTCVHLALMKGHEQTLHLLLQQFPRFINHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWVMDTNGDTPLHVCLEYGSISCMKMLLNEGEVSLDDNVRDKGNWKPIDVAQTFEVGNIYSKVLKEVKKKGPPLGAGKKPSSFRTPILNAKATFEDGPSPVLSMNSPYSLYSNNSPLPVLPRRISTHTTSGNGGNRRSSITNPVFNPRKPTLSTDSFSSSSNSSSRLRVNSINVKTPVGVSPKKELVSESVRHSATPTSPHNNIALINRYLLPNKSNDNVRGDSQTATINDDGGGGNGGDATIGMGLRKDPDDENENKYKIKVNNGEPRRRVSLLNIPISKLRNSNNTRAED 0A17E78BF449CBFE kog1_yeast Target of rapamycin complex 1 subunit KOG1 Kontroller of growth protein 1 Local anesthetic-sensitive protein 24 YHR186C KOG1 LAS24 H9998.14 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPEIYGPQPLKPLNTVMRHGFEEQYQSDQLLQSLANDFIFYFDDKRHKTNGNPIPEEDKQRDVNRYYQPITDWKIMKDRQKTVSAALLLCLNLGVDPPDVMKTHPCARVEAWVDPLNFQDSKKAIEQIGKNLQAQYETLSLRTRYKQSLDPCVEDVKRFCNSLRRTSKEDRILFHYNGHGVPKPTKSGEIWVFNRGYTQYIPVSLYDLQTWLGAPCIFVYDCNSAENILINFQKFVQKRIKDDEEGNHDVAAPSPTSAYQDCFQLASCTSDELLLMSPELPADLFSCCLTCPIEISIRIFLMQSPLKDSKYKIFFENSTSNQPFGDSKNSFKSKIPNVNIPGMLSDRRTPLGELNWIFTAITDTIAWTSLPRPLFKKLFRHDLMIAALFRNFLLAKRIMPWYNCHPVSDPELPDSITTHPMWKSWDLAMDEVLTKIVIDLKNAPPATALESQMILQQQETLQNGGSSKSNAQDTKAGSIQTQSRFAVANLSTMSLVNNPALQSRKSISLQSSQQQLQQQQQQQQQFTGFFEQNLTAFELWLKYASNVRHPPEQLPIVLQVLLSQVHRIRALVLLSRFLDLGPWAVYLSLSIGIFPYVLKLLQSPAPELKPILVFIWARIMSIDYKNTQSELIKEKGYMYFVTVLVPDWGVNGMSATNGSAMINSGNPLTMTASQNINGPSSRYYERQQGNRTSNLGHNNLPFYHSNDTTDEQKAMAVFVLASFVRNFPLGQKNCFSLELVNKLCFYIDNSEIPLLRQWCVILLGLLFADNPLNRFVCMNTGAVEILLKSLKDPVPEVRTASIFALKHFISGFQDAEVILRLQQEFEEQYQQLHSQLQHLQNQSHLQQQQSQQQQQHLEQQQMKIEKQIRHCQVMQNQLEVIDLRKLKRQEIGNLISILPLINDGSSLVRKELVVYFSHIVSRYSNFFIVVVFNDLLEEIKLLEKSDINTRNTSDKYSVSQGSIFYTVWKSLLILAEDPFLENKELSKQVIDYILLELSAHKELGGPFAVMEKFLLKRSSKAHQTGKFGFNSSQVQFVKSSLRSFSPNERVDNNAFKKEQQQHDPKISHPMRTSLAKLFQSLGFSESNSDSDTQSSNTSMKSHTSKKGPSGLYLLNGNNNIYPTAETPRFRKHTEPLQLPLNSSFLDYSREYFQEPQMKKQEADEPGSVEYNARLWRRNRNETIIQETQGEKKLSIYGNWSKKLISLNNKSQPKLMKFAQFEDQLITADDRSTITVFDWEKGKTLSKFSNGTPFGTKVTDLKLINEDDSALLLTGSSDGVIKIYRDYQDVDTFKIVSAWRGLTDMLLTPRSTGLLTEWLQIRGSLLTTGDVKVIRVWDAHTETVEVDIPAKTSSLITSLTADQLAGNIFVAGFADGSLRVYDRRLDPRDSMIRRWRAGNDKQGVWINNVHLQRGGYRELVSGATNGVVELWDIRSEDPVESFVDQNVTSQYGSQQKPTTMTCMQVHEHAPIIATGTKQIKIWTTSGDLLNSFKNSHNNGVTSTLAATGIPKSLSYSSTSDAFLSSMAFHPHRMMIAATNSHDSIVNIYKCEDERIDYF B927EE93700176D3 B927EE93700176D3 tor2_yeast Serine/threonine-protein kinase TOR2 DRR2 TSC14 YKL203C TOR2 Phosphatidylinositol 4-kinase TOR2 Target of rapamycin kinase 2 Dominant rapamycin resistance protein 2 Temperature-sensitive CSG2 suppressor protein 14 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNKYINKYTTPPNLLSLRQRAEGKHRTRKKLTHKSHSHDDEMSTTSNTDSNHNGPNDSGRVITGSAGHIGKISFVDSELDTTFSTLNLIFDKLKSDVPQERASGANELSTTLTSLAREVSAEQFQRFSNSLNNKIFELIHGFTSSEKIGGILAVDTLISFYLSTEELPNQTSRLANYLRVLIPSSDIEVMRLAANTLGRLTVPGGTLTSDFVEFEVRTCIDWLTLTADNNSSSSKLEYRRHAALLIIKALADNSPYLLYPYVNSILDNIWVPLRDAKLIIRLDAAVALGKCLTIIQDRDPALGKQWFQRLFQGCTHGLSLNTNDSVHATLLVFRELLSLKAPYLRDKYDDIYKSTMKYKEYKFDVIRREVYAILPLLAAFDPAIFTKKYLDRIMVHYLRYLKNIDMNAANNSDKPFILVSIGDIAFEVGSSISPYMTLILDNIREGLRTKFKVRKQFEKDLFYCIGKLACALGPAFAKHLNKDLLNLMLNCPMSDHMQETLMILNEKIPSLESTVNSRILNLLSISLSGEKFIQSNQYDFNNQFSIEKARKSRNQSFMKKTGESNDDITDAQILIQCFKMLQLIHHQYSLTEFVRLITISYIEHEDSSVRKLAALTSCDLFIKDDICKQTSVHALHSVSEVLSKLLMIAITDPVAEIRLEILQHLGSNFDPQLAQPDNLRLLFMALNDEIFGIQLEAIKIIGRLSSVNPAYVVPSLRKTLLELLTQLKFSNMPKKKEESATLLCTLINSSDEVAKPYIDPILDVILPKCQDASSAVASTALKVLGELSVVGGKEMTRYLKELMPLIINTFQDQSNSFKRDAALTTLGQLAASSGYVVGPLLDYPELLGILINILKTENNPHIRRGTVRLIGILGALDPYKHREIEVTSNSKSSVEQNAPSIDIALLMQGVSPSNDEYYPTVVIHNLMKILNDPSLSIHHTAAIQAIMHIFQNLGLRCVSFLDQIIPGIILVMRSCPPSQLDFYFQQLGSLISIVKQHIRPHVEKIYGVIREFFPIIKLQITIISVIESISKALEGEFKRFVPETLTFFLDILENDQSNKRIVPIRILKSLVTFGPNLEDYSHLIMPIVVRMTEYSAGSLKKISIITLGRLAKNINLSEMSSRIVQALVRILNNGDRELTKATMNTLSLLLLQLGTDFVVFVPVINKALLRNRIQHSVYDQLVNKLLNNECLPTNIIFDKENEVPERKNYEDEMQVTKLPVNQNILKNAWYCSQQKTKEDWQEWIRRLSIQLLKESPSACLRSCSSLVSVYYPLARELFNASFSSCWVELQTSYQEDLIQALCKALSSSENPPEIYQMLLNLVEFMEHDDKPLPIPIHTLGKYAQKCHAFAKALHYKEVEFLEEPKNSTIEALISINNQLHQTDSAIGILKHAQQHNELQLKETWYEKLQRWEDALAAYNEKEAAGEDSVEVMMGKLRSLYALGEWEELSKLASEKWGTAKPEVKKAMAPLAAGAAWGLEQWDEIAQYTSVMKSQSPDKEFYDAILCLHRNNFKKAEVHIFNARDLLVTELSALVNESYNRAYNVVVRAQIIAELEEIIKYKKLPQNSDKRLTMRETWNTRLLGCQKNIDVWQRILRVRSLVIKPKEDAQVRIKFANLCRKSGRMALAKKVLNTLLEETDDPDHPNTAKASPPVVYAQLKYLWATGLQDEALKQLINFTSRMAHDLGLDPNNMIAQSVPQQSKRVPRHVEDYTKLLARCFLKQGEWRVCLQPKWRLSNPDSILGSYLLATHFDNTWYKAWHNWALANFEVISMLTSVSKKKQEGSDASSVTDINEFDNGMIGVNTFDAKEVHYSSNLIHRHVIPAIKGFFHSISLSESSSLQDALRLLTLWFTFGGIPEATQAMHEGFNLIQIGTWLEVLPQLISRIHQPNQIVSRSLLSLLSDLGKAHPQALVYPLMVAIKSESLSRQKAALSIIEKMRIHSPVLVDQAELVSHELIRMAVLWHEQWYEGLDDASRQFFGEHNTEKMFAALEPLYEMLKRGPETLREISFQNSFGRDLNDAYEWLMNYKKSKDVSNLNQAWDIYYNVFRKIGKQLPQLQTLELQHVSPKLLSAHDLELAVPGTRASGGKPIVKISKFEPVFSVISSKQRPRKFCIKGSDGKDYKYVLKGHEDIRQDSLVMQLFGLVNTLLQNDAECFRRHLDIQQYPAIPLSPKSGLLGWVPNSDTFHVLIREHREAKKIPLNIEHWVMLQMAPDYDNLTLLQKVEVFTYALNNTEGQDLYKVLWLKSRSSETWLERRTTYTRSLAVMSMTGYILGLGDRHPSNLMLDRITGKVIHIDFGDCFEAAILREKFPEKVPFRLTRMLTYAMEVSGIEGSFRITCENVMKVLRDNKGSLMAILEAFAFDPLINWGFDLPTKKIEEETGIQLPVMNANELLSNGAITEEEVQRVENEHKNAIRNARAMLVLKRITDKLTGNDIRRFNDLDVPEQVDKLIQQATSVENLCQHYIGWCPFW F2349690146058CF F2349690146058CF tsc11_yeast Target of rapamycin complex 2 subunit TSC11 AVO3 YER093C TSC11 Adheres voraciously to TOR2 protein 3 Temperature-sensitive CSG2 suppressor protein 11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSIPHSAKQSSPLSSRRRSVTNTTPLLTPRHSRDNSSTQISSAKNITSSSPSTITNESSKRNKQNLVLSTSFISTKRLENSAPSPTSPLMARRTRSTMTKALLNLKAEINNQYQELARLRKKKDDIEHLRDSTISDIYSGSYSTNHLQKHSMRIRANTQLREIDNSIKRVEKHIFDLKQQFDKKRQRSLTTSSSIKADVGSIRNDDGQNNDSEELGDHDSLTDQVTLDDEYLTTPTSGTERNSQQNLNRNSTVNSRNNENHSTLSIPDLDGSNKVNLTGDTEKDLGDLENENQIFTSTTTEAATWLVSDYMQSFQEKNVNPDFIAQKANGLVTLLKEHSEIRKDLVLTSFMSSIQNLLLNGNKLIAASAYRVCRYLINSSIFIDELLELRLDAFIIISLAKDNSFQIEREQALKMVRRFIEYNNGVTQGIMQAIISCVEKPEDSLRHMALETLLELCFVAPEMVKECRGMRVIEGFLQDYTSFSLASVILDTILQLMATHKTRQHFLEDFNVSVLTTVFSDTNTKSNVNVEKMQNASTLISITLNSYNGFMLFSNNNFKPLKQLVSFFQIPICAQYLIDIFLDVLKIKPLPYKPRGRHSHSFKPIPSQYYKECMSVNQRLALIVLILENSEFVPHLLELLNEEDRDDHLVAKGRYLLTEYFNLRMNLVDKKYTSVSKPIYKENFTYVNETFQFKKIAYKMNRNRNTIGMSGIDYAQNIKSFSKNIKENTLLREVDDFRFRRMVYDSKVLQTKDFTRWNWNIINELLEGPLLNKKQLEELVKSTKFIRRLLVFYRPLRLRFSNVNKGAKLSQKYVQVGCQFFKTLTATPEGMKILMDDTKIIPQLASLMFRAMEGNISGNIFNKNKLREKIIFGYFKFIGILTQSKNGVHILTRWNFFTVIYKMFQFESKLGLEFLLLTIPELDLKYSSHCRVIIGKALVVANEKVRIEATKHIGDKLKELLSTKESDLKLKANKVKLQQFKMEMLTRQLYDLSPSVVAVADQALYECIVAGNGSEELGTSFRMFLNQMVFIRSPILFELLSRPYGFQLLNEINFVKEERDSWLSKKNIEYVHIVEEFLKKNESINAKSLTFQQKSRLPLHFYESLTKTEDGILLLSQTGDLVTFMNVIKKYVNGNNMATVENAKEILDLKAALWCVGFIGSTELGIGLLDNYSLVEDIIEVAYNASVTSVRFTAFYVLGLISMTREGCEILDEMGWNCCVSVQDEPIGIALPNRLDRFLSYNEHKWSAFGEYSDEMIVFNKSDGDLIEKCLPIEFDLDKLLKEKDTAENPLNEKIITNKYDNDITSQTITVSGENSSLFANEGLSSPYVTQYRNDDDSIESKVLHIVSQLGNHILSNHAVKEITEINNKYGPRLFENEKMFFKVFNMMSKYRFKPHVRKFLCGLFINNRALENVIRHDNKRDKRPANFTR 07BB5D75BA204E50 07BB5D75BA204E50 avo1_yeast Target of rapamycin complex 2 subunit AVO1 Adheres voraciously to TOR2 protein 1 YOL078W AVO1 O1110 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDTVTVLNELRAQFLRVCPEKDQMKRIIKPYIPVDEFNTEQCLDSSIRELYMNSDGVSLLPELESPPVSKDFMENYASLGKMRIMRENEGQKGKANQNLIGAEKTERDEEETRNLQDKSAKNTLIVEENGTLRYNPLNSSASNSLLNDDDHTSGKHHKTSSKEDSYLNSSMEMQKKSSKRSSLPFVRIFKSRRDHSNTSGNKNVMNTTNTRAKSSTLHPPGARHNKKGSKFDMNFDFDENLEEEDDDDDDDEEGDDIHSQFFQLDDDFDAKGSGASPAHKGINGMSNNKNNTYTNNRNSISILDDRESSNGNIGSASRLKSHFPTSQKGKIFLTDNKNDGQKSDSLNANKGIHGDGSSASGNGSVSRDGLTETESNNISDMESYINEKDLDDLNFDTVTSNINKTVSDLGGHESTNDGTAVMNRDSKDSRSNSNEFNAQNRDRITPGSSYGKSLLGSEYSEERYSNNDSSTMESGEMSLDSDMQTNTIPSHSIPMSMQKYGIYHGDDDSTLNNVFDKAVLTMNSSRHPKERRDTVISGKEPTSLTSSNRKFSVSSNLTSTRSPLLRGHGRTSSTASSEHMKAPKVSDSVLHRARKSTLTLKQDHSQPSVPSSVHKSSKEGNILIEKTTDYLVSKPKASQLSNMFNKKKKRTNTNSVDVLEYFSFVCGDKVPNYESMGLEIYIQASKKYKRNSFTTKVRKSSTIFEVIGFALFLYSTEKKPDNFEEDGLTVEDISNPNNFSLKIVDEDGEPFEDNFGKLDRKSTIQSISDSEVVLCKVDDAEKSQNEIETPLPFETGGGLMDASTLDANSSHDTTDGTINQLSFYKPIIGNEDDIDKTNGSKIIDVTVYLYPNVNPKFNYTTISVLVTSHINDILVKYCKMKNMDPNEYALKVLGKNYILDLNDTVLRLDGINKVELISKKDARELHLEKMKPDLKKPVLPTIQSNDLTPLTLEPLNSYLKADAGGAVAAIPENTKVTSKAKKISTKYKLGLAKQHSSSSVASGSVSTAGGLANGNGFFKNKNSSKSSLHGTLQFHNINRSQSTMEHTPDTPNGVGDNFQDLFTGAYHKYKVWRRQQMSFINKHERTLAIDGDYIYIVPPEGRIHWHDNVKTKSLHISQVVLVKKSKRVPEHFKIFVRREGQDDIKRYYFEAVSGQECTEIVTRLQNLLSAYRMNHK ED2B30D014EE5652 ED2B30D014EE5652 tor1_yeast Serine/threonine-protein kinase TOR1 DRR1 TOR1 YJR066W J1803 Phosphatidylinositol kinase homolog TOR1 Target of rapamycin kinase 1 Dominant rapamycin resistance protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEPHEEQIWKSKLLKAANNDMDMDRNVPLAPNLNVNMNMKMNASRNGDEFGLTSSRFDGVVIGSNGDVNFKPILEKIFRELTSDYKEERKLASISLFDLLVSLEHELSIEEFQAVSNDINNKILELVHTKKTSTRVGAVLSIDTLISFYAYTERLPNETSRLAGYLRGLIPSNDVEVMRLAAKTLGKLAVPGGTYTSDFVEFEIKSCLEWLTASTEKNSFSSSKPDHAKHAALLIITALAENCPYLLYQYLNSILDNIWRALRDPHLVIRIDASITLAKCLSTLRNRDPQLTSQWVQRLATSCEYGFQVNTLECIHASLLVYKEILFLKDPFLNQVFDQMCLNCIAYENHKAKMIREKIYQIVPLLASFNPQLFAGKYLHQIMDNYLEILTNAPANKIPHLKDDKPQILISIGDIAYEVGPDIAPYVKQILDYIEHDLQTKFKFRKKFENEIFYCIGRLAVPLGPVLGKLLNRNILDLMFKCPLSDYMQETFQILTERIPSLGPKINDELLNLVCSTLSGTPFIQPGSPMEIPSFSRERAREWRNKNILQKTGESNDDNNDIKIIIQAFRMLKNIKSRFSLVEFVRIVALSYIEHTDPRVRKLAALTSCEIYVKDNICKQTSLHSLNTVSEVLSKLLAITIADPLQDIRLEVLKNLNPCFDPQLAQPDNLRLLFTALHDESFNIQSVAMELVGRLSSVNPAYVIPSIRKILLELLTKLKFSTSSREKEETASLLCTLIRSSKDVAKPYIEPLLNVLLPKFQDTSSTVASTALRTIGELSVVGGEDMKIYLKDLFPLIIKTFQDQSNSFKREAALKALGQLAASSGYVIDPLLDYPELLGILVNILKTENSQNIRRQTVTLIGILGAIDPYRQKEREVTSTTDISTEQNAPPIDIALLMQGMSPSNDEYYTTVVIHCLLKILKDPSLSSYHTAVIQAIMHIFQTLGLKCVSFLDQIIPTILDVMRTCSQSLLEFYFQQLCSLIIIVRQHIRPHVDSIFQAIKDFSSVAKLQITLVSVIEAISKALEGEFKRLVPLTLTLFLVILENDKSSDKVLSRRVLRLLESFGPNLEGYSHLITPKIVQMAEFTSGNLQRSAIITIGKLAKDVDLFEMSSRIVHSLLRVLSSTTSDELSKVIMNTLSLLLIQMGTSFAIFIPVINEVLMKKHIQHTIYDDLTNRILNNDVLPTKILEANTTDYKPAEQMEAADAGVAKLPINQSVLKSAWNSSQQRTKEDWQEWSKRLSIQLLKESPSHALRACSNLASMYYPLAKELFNTAFACVWTELYSQYQEDLIGSLCIALSSPLNPPEIHQTLLNLVEFMEHDDKALPIPTQSLGEYAERCHAYAKALHYKEIKFIKEPENSTIESLISINNQLNQTDAAIGILKHAQQHHSLQLKETWFEKLERWEDALHAYNEREKAGDTSVSVTLGKMRSLHALGEWEQLSQLAARKWKVSKLQTKKLIAPLAAGAAWGLGEWDMLEQYISVMKPKSPDKEFFDAILYLHKNDYDNASKHILNARDLLVTEISALINESYNRAYSVIVRTQIITEFEEIIKYKQLPPNSEKKLHYQNLWTKRLLGCQKNVDLWQRVLRVRSLVIKPKQDLQIWIKFANLCRKSGRMRLANKALNMLLEGGNDPSLPNTFKAPPPVVYAQLKYIWATGAYKEALNHLIGFTSRLAHDLGLDPNNMIAQSVKLSSASTAPYVEEYTKLLARCFLKQGEWRIATQPNWRNTNPDAILGSYLLATHFDKNWYKAWHNWALANFEVISMVQEETKLNGGKNDDDDDTAVNNDNVRIDGSILGSGSLTINGNRYPLELIQRHVVPAIKGFFHSISLLETSCLQDTLRLLTLLFNFGGIKEVSQAMYEGFNLMKIENWLEVLPQLISRIHQPDPTVSNSLLSLLSDLGKAHPQALVYPLTVAIKSESVSRQKAALSIIEKIRIHSPVLVNQAELVSHELIRVAVLWHELWYEGLEDASRQFFVEHNIEKMFSTLEPLHKHLGNEPQTLSEVSFQKSFGRDLNDAYEWLNNYKKSKDINNLNQAWDIYYNVFRKITRQIPQLQTLDLQHVSPQLLATHDLELAVPGTYFPGKPTIRIAKFEPLFSVISSKQRPRKFSIKGSDGKDYKYVLKGHEDIRQDSLVMQLFGLVNTLLKNDSECFKRHLDIQQYPAIPLSPKSGLLGWVPNSDTFHVLIREHRDAKKIPLNIEHWVMLQMAPDYENLTLLQKIEVFTYALDNTKGQDLYKILWLKSRSSETWLERRTTYTRSLAVMSMTGYILGLGDRHPSNLMLDRITGKVIHIDFGDCFEAAILREKYPEKVPFRLTRMLTYAMEVSGIEGSFRITCENVMRVLRDNKESLMAILEAFALDPLIHWGFDLPPQKLTEQTGIPLPLINPSELLRKGAITVEEAANMEAEQQNETKNARAMLVLRRITDKLTGNDIKRFNELDVPEQVDKLIQQATSIERLCQHYIGWCPFW ACB1781B9963BB1E ACB1781B9963BB1E tco89_yeast Target of rapamycin complex 1 subunit TCO89 YPL180W TCO89 89 kDa TOR complex 1 protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MVHRGRTLKSDTDVTSLNASTVSHQSKPFRQFSTRSRAKSNASFKGLRRVLTHDGTLDNDYFNKHNVSQKCKSSDALFRKRTISGLNMTALTRVKSNQGKRSASFHSPVHNTLLSPKNSSHSNTGTAGFGLKPRRSKSTQSVLSLRDAQESKKSESTTDEEVECFSEDNIEDGKVNNDKVIAEHVMPEEKKNVQQLNQNELQSPDSIDEQEEDKSGTDGKENHRAVSLPLPHLSSNNYFGESSHSIEHQKDGETSPSSIETKLNATSVINEEGQSKVTKEADIDDLSSHSQNLRASLVKAGDNISEAPYDKEKKILDVGNTLAAHKSNQKPSHSDEQFDQEDHIDAPRSNSSRKSDSSFMSLRRQSSKQHKLLNEEEDLIKPDDISSAGTKDIEGHSLLENYAPNMILSQSTGVERRFENSSSIQNSLGNEIHDSGEHMASGDTFNELDDGKLRKSKKNGGRSQLGQNIPNSQSTFPTIANIGSKDNNVPQHNFSTSISSLTNNLRRAAPESFHGSRMNNIFHKKGNQNLLLRSNDLNKNSAAPASPLSNEHITSSTNSGSDANRQSNSGAKFNSFAQFLKSDGIDAESRTQRKLWLQRENSIMDLSSQNDGSDSIFMAGNIDAKREFERISHEYSNVKRFYNPLDEALLRVQPIITGNANNIRKKSHNDAQSIAHSSSDTDHKDEDDLLFTNYDKKFDDLYPHLASAKIQAVLSGIWKSESYLFNKDVNPINKNRTTSTNHSVGHTASQNARNLLRGPMGSSTTLHHQRVINSLQPTTRAVNRRMENVGYMHTQPQQR 7243C08F4C74633D tor1-lst8-5 2 6 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 1, Table III tor2-lst8-1 2 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey association association Fig. 1, Table III kog1-lst8-1 2 6 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey association association Fig. 1, Table III lst8-tor1-1 2 3 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association Fig. 1, Table III tor2-bit61 1 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association The results showed that HA3-Tor2p but not Tor1p co-precipitated with Bit61p-Myc (Fig. 1C). Fig. 1C tor1-tco89-1 1 11 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association By contrast, Tor1p but not HA3-Tor2p co-precipitated with Tco89-Myc (Fig. 1D) Fig. 1D