IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids: substrate not required. Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids: substrate not required. J. Cell Biol. (0021-9525) 2003 imex curation Moore M., Goforth RL., Mori H., Henry R. It is not clear from the text what antibodies were used to detect co-precipitating proteins , therefore the detection method is described as 'western blot'. The proteins cpSRP, cpFtsY were incubated with salt-washed thylakoids, and the various nucleotides as described in the text. The membranes were washed, solubilized with maltoside. Co-immunoprecipitation was carried out. Western blotting was carried out. Ralph.Henry@uark.edu 23-Dec-2009: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Moore M., Goforth RL., Mori H., Henry R. It is not clear from the text what antibodies were used to detect co-precipitating proteins , therefore the detection method is described as 'western blot'. It is not clear from the text what antibodies were used to detect co-precipitating proteins , therefore the detection method is described as 'western blot'. The proteins cpSRP, cpFtsY were incubated with salt-washed thylakoids, and the various nucleotides as described in the text. The membranes were washed, solubilized with maltoside. Co-immunoprecipitation was carried out. Western blotting was carried out. Ralph.Henry@uark.edu Accepted 2009-DEC-2 by ORCHARD. 23-Dec-2009: Contacted by JYOTI. J. Cell Biol. (0021-9525) 2003 orchard Only protein-protein interactions imex curation Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids: substrate not required. Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids: substrate not required. J. Cell Biol. (0021-9525) 2003 imex curation Moore M., Goforth RL., Mori H., Henry R. It is not clear from the text what antibodies were used to detect co-precipitating proteins , therefore the detection method is described as 'western blot'. The proteins cpSRP, cpFtsY were incubated with salt-washed thylakoids, and the various nucleotides as described in the text. The membranes were washed, solubilized with maltoside. Co-immunoprecipitation was carried out. Western blotting was carried out. Ralph.Henry@uark.edu 23-Dec-2009: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Moore M., Goforth RL., Mori H., Henry R. It is not clear from the text what antibodies were used to detect co-precipitating proteins , therefore the detection method is described as 'western blot'. It is not clear from the text what antibodies were used to detect co-precipitating proteins , therefore the detection method is described as 'western blot'. The proteins cpSRP, cpFtsY were incubated with salt-washed thylakoids, and the various nucleotides as described in the text. The membranes were washed, solubilized with maltoside. Co-immunoprecipitation was carried out. Western blotting was carried out. Ralph.Henry@uark.edu Accepted 2009-DEC-2 by ORCHARD. 23-Dec-2009: Contacted by JYOTI. J. Cell Biol. (0021-9525) 2003 orchard Only protein-protein interactions imex curation sr43c_arath Signal recognition particle 43 kDa protein, chloroplastic CAO T30B22.25 At2g47450 Chromo protein SRP43 CPSRP43 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQKVFLAMDTCALVIHQSLSRIKLSPPKSSSSSSSAFSPESLPIRRIELCFRGAICAAVQRNYEETTSSVEEAEEDDESSSSYGEVNKIIGSRTAGEGAMEYLIEWKDGHSPSWVPSSYIAADVVSEYETPWWTAARKADEQALSQLLEDRDVDAVDENGRTALLFVAGLGSDKCVRLLAEAGADLDHRDMRGGLTALHMAAGYVRPEVVEALVELGADIEVEDERGLTALELAREILKTTPKGNPMQFGRRIGLEKVINVLEGQVFEYAEVDEIVEKRGKGKDVEYLVRWKDGGDCEWVKGVHVAEDVAKDYEDGLEYAVAESVIGKRVGDDGKTIEYLVKWTDMSDATWEPQDNVDSTLVLLYQQQQPMNE DIP protein O22265 original sequence version: 82 DIP protein O22265 original sequence version: 98 F75ED9C7046A1441 scy1_arath Preprotein translocase subunit SCY1, chloroplastic SCY1 CpSecY SECY MSF3.9 At2g18710 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MITVSEVSSYSSSSSNFASLSRLNHKSSSRLRSSSLYKGSFFSVSTKTRRNTCKAKSWNLGLVINSRSSEASVFDPLGINPDETSGLSSIWESFVSLLSPSFESSSGNRRDKPSSGRGVAAAIEDSSIDFGDFFKGPLPGKFLKLLGFLALSRLGIYIPLGGVNREAFVGNLDQNSILSTLDTFSGGGIGRLGICSLGIVPFINAQIVFQLLAQVYPKLQDLQKKEGEAGRKKILQYTRYASVGFAIVQAIGQVFYLRPYVNDFSTEWVVSSVTLLTLGSVLTTYIGERISDLKLGNGTSLLIFTSIISYLPASFGRTTAEALQEGNYTGLGTIVVSFLLLVLGIVYVQEAERKIPLNYASRYTSKAGGLQKSAYLPFKVNSAGVMPIIFSTSSLALPATLARFTGISALKNVAFALTPGGSFYLPTNILLIAFFNYYYTFLQLDPDDVSEQLKRQGASIPLVRPGKSTALFIKTVLGRISVLGSAFLAVLAAGPAVVEQITHLTAFRGFAGTSVLILVGCATDTARKVQAEIISQKYKNIEFYELDKYDP 526854797A3B8059 alb3_arath Inner membrane protein ALBINO3, chloroplastic ALB3 F8N16.9 At2g28800 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MARVLVSSPSSFFGSPLIKPSSSLRHSGVGGGGTAQFLPYRSNNNKLFTTSTTVRFSLNEIPPFHGLDSSVDIGAIFTRAESLLYTIADAAVVGADSVVTTDSSAVQKSGGWFGFISDAMELVLKILKDGLSAVHVPYAYGFAIILLTIIVKAATYPLTKQQVESTLAMQNLQPKIKAIQQRYAGNQERIQLETSRLYKQAGVNPLAGCLPTLATIPVWIGLYQALSNVANEGLFTEGFFWIPSLGGPTSIAARQSGSGISWLFPFVDGHPPLGWYDTVAYLVLPVLLIASQYVSMEIMKPPQTDDPAQKNTLLVFKFLPLMIGYFALSVPSGLSIYWLTNNVLSTAQQVYLRKLGGAKPNMDENASKIISAGRAKRSIAQPDDAGERFRQLKEQEKRSKKNKAVAKDTVELVEESQSESEEGSDDEEEEAREGALASSTTSKPLPEVGQRRSKRSKRKRTV 2BBF7AF5BA2E6DDE cftsy_arath Cell division protein FtsY homolog, chloroplastic CPFTSY At2g45770 FTSY Chloroplast SRP receptor homolog, alpha subunit CpFtsY Fused signal recognition particle receptor F4I18.25 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATSSAHLSFLAGRISPFSSERIGLFPLRGEFRPRMTRFRCSAGPSGFFTRLGRLIKEKAKSDVEKVFSGFSKTRENLAVIDELLLFWNLAETDRVLDELEEALLVSDFGPKITVRIVERLREDIMSGKLKSGSEIKDALKESVLEMLAKKNSKTELQLGFRKPAVIMIVGVNGGGKTTSLGKLAHRLKNEGTKVLMAAGDTFRAAASDQLEIWAERTGCEIVVAEGDKAKAATVLSKAVKRGKEEGYDVVLCDTSGRLHTNYSLMEELIACKKAVGKIVSGAPNEILLVLDGNTGLNMLPQAREFNEVVGITGLILTKLDGSARGGCVVSVVEELGIPVKFIGVGEAVEDLQPFDPEAFVNAIFS 8986F431A6A3BE12 sr54c_arath Signal recognition particle 54 kDa protein, chloroplastic FFC At5g03940 F8F6_150 FFC CPSRP54 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEALQFSSVNRVPCTLSCTGNRRIKAAFSSAFTGGTINSASLSSSRNLSTREIWSWVKSKTVVGHGRYRRSQVRAEMFGQLTGGLEAAWSKLKGEEVLTKDNIAEPMRDIRRALLEADVSLPVVRRFVQSVSDQAVGMGVIRGVKPDQQLVKIVHDELVKLMGGEVSELQFAKSGPTVILLAGLQGVGKTTVCAKLACYLKKQGKSCMLIAGDVYRPAAIDQLVILGEQVGVPVYTAGTDVKPADIAKQGLKEAKKNNVDVVIMDTAGRLQIDKGMMDELKDVKKFLNPTEVLLVVDAMTGQEAAALVTTFNVEIGITGAILTKLDGDSRGGAALSVKEVSGKPIKLVGRGERMEDLEPFYPDRMAGRILGMGDVLSFVEKATEVMRQEDAEDLQKKIMSAKFDFNDFLKQTRAVAKMGSMTRVLGMIPGMGKVSPAQIREAEKNLLVMEAMIEVMTPEERERPELLAESPERRKRIAKDSGKTEQQVSALVAQIFQMRVKMKNLMGVMEGGSIPALSGLEDALKAEQKAPPGTARRKRKADSRKKFVESASSKPGPRGFGSGN DIP protein P37107 original sequence version: 105 423F7285FB9063E4 ftsy-srp54-srp43-a 1 7 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 6 unspecified role unspecified role bait bait region Trx tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) association association Figure 1 Figure 2 Figure 3A The authors demonstrated "the quantity of cpSRP coprecipitated with cpFtsY was elevated four- to fivefold in the presence of GMP-PNP relative to assays conducted with no nucleotide, GTP, GDP, ATP, or AMP-PNP ". However this effect was not seen on interaction of ALB3. Details of constructs used were obtained from references provided in the text. guanosine 5' [beta gamma] -imido triphosphate (GMP-PNP) srp43-ftsy 1 4 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 6 unspecified role unspecified role prey prey region Trx tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 7 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) association association Figure 3 Details of constructs used were obtained from references provided in the text. Western blots of the coprecipitating proteins indicate that cpFtsY alone associates with ALB3, but the association is enhanced more than sixfold by the addition of cpSRP srp43-ftsy-1 1 7 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Figure 4 srp43-ftsy-2 2 6 unspecified role unspecified role prey prey region Trx tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 7 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) association association Figure 4 Details of constructs used were obtained from references provided in the text. ALB3, cpSRP54, and cpFtsY were all coprecipitated with cpSRP43-FLAG in the presence of cross-linker. srp43-ftsy-3 1 5 unspecified role unspecified role unspecified role unspecified role arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 6 unspecified role unspecified role unspecified role unspecified role region Trx tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Figure 3 Details of constructs used were obtained from references provided in the text. Western blots of the coprecipitating proteins indicate that cpFtsY alone associates with ALB3, but the association is enhanced more than sixfold by the addition of cpSRP It is unclear which of these proteins is binding to the S-Agarose.