UniProt UniProt http://www.uniprot.org Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling. Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling. Cell (0092-8674) 2003 imex curation Wang Y., Shirogane T., Liu D., Harper JW., Elledge SJ. selledge@bcm.tmc.edu 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang Y., Shirogane T., Liu D., Harper JW., Elledge SJ. selledge@bcm.tmc.edu Accepted 2009-FEB-19 by JYOTI. 03-Mar-2009: Contacted by JYOTI. Cell (0092-8674) 2003 jyoti Only protein-protein interactions imex curation Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling. Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling. Cell (0092-8674) 2003 imex curation Wang Y., Shirogane T., Liu D., Harper JW., Elledge SJ. selledge@bcm.tmc.edu 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Wang Y., Shirogane T., Liu D., Harper JW., Elledge SJ. selledge@bcm.tmc.edu Accepted 2009-FEB-19 by JYOTI. 03-Mar-2009: Contacted by JYOTI. Cell (0092-8674) 2003 jyoti Only protein-protein interactions imex curation Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling. Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling. Cell (0092-8674) 2003 imex curation Wang Y., Shirogane T., Liu D., Harper JW., Elledge SJ. selledge@bcm.tmc.edu 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Wang Y., Shirogane T., Liu D., Harper JW., Elledge SJ. selledge@bcm.tmc.edu Accepted 2009-FEB-19 by JYOTI. 03-Mar-2009: Contacted by JYOTI. Cell (0092-8674) 2003 jyoti Only protein-protein interactions imex curation Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling. Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling. Cell (0092-8674) 2003 imex curation Wang Y., Shirogane T., Liu D., Harper JW., Elledge SJ. selledge@bcm.tmc.edu 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang Y., Shirogane T., Liu D., Harper JW., Elledge SJ. selledge@bcm.tmc.edu Accepted 2009-FEB-19 by JYOTI. 03-Mar-2009: Contacted by JYOTI. Cell (0092-8674) 2003 jyoti Only protein-protein interactions imex curation tem1_yeast Protein TEM1 YML064C TEM1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MATPSTGANNSIPAVRNQVEVQVGLVGDAQVGKTSLMVKYVQNIYDKEYTQTLGVNFLKRKVSIRSTDIIFSIMDLGGQREFINMLPIATVGSSVIIFLFDLTRPETLSSIKEWYRQAYGLNDSAIPILVGTKYDLLIDLDPEYQEQISRTSMKYAQVMNAPLIFCSTAKSINIQKIFKIALAKIFNLTLTIPEINEIGDPLLIYKHLGGQQHRHHNKSQDRKSHNIRKPSSSPSSKAPSPGVNT F650C8946A03707C F650C8946A03707C amn1_yeast Antagonist of mitotic exit network protein 1 CST13 YBR158W YBR1208 AMN1 ICS4 Chromosome stability protein 13 Increased copper-sensitivity protein 4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKLERVSSNGSFKRGRDIQSLESPCTRPLKKMSPSPSFTSLKMEKPFKDIVRKYGGHLHQSSYNPGSSKVELVRPDLSLKTDQSFLQSSVQTTPNKKSCNEYLSTPEATPLKNTATENAWATSRVVSASSLSIVTPTEIKNILVDEFSELKLGQPLTAQHQRSHAVFEIPEIVENIIKMIVSLESANIPKERPCLRRNPQSYEHSLLMYKDEERAKKAWSAAQQLRDPPLVGHKEKKQGALFSCMMVNRLWLNVTRPFLFKSLHFKSVHNFKEFLRTSQETTQVMRPSHFILHKLHQVTQPDIERLSRMECQNLKWLEFYVCPRITPPLSWFDNLHKLEKLIIPGNKNIDDNFLLRLSQSIPNLKHLVLRACDNVSDSGVVCIALNCPKLKTFNIGRHRRGNLITSVSLVALGKYTQVETVGFAGCDVDDAGIWEFARLNGKNVERLSLNSCRLLTDYSLPILFALNSFPNLAVLEIRNLDKITDVRHFVKYNLWKKSLDAPILIEACERITKLIDQEENRVKRINSLVALKDMTAWVNADDEIENNVD EA1EC675D6A84F20 EA1EC675D6A84F20 cdc15_yeast Cell division control protein 15 CDC15 LYT1 YAR019C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNSMADTDRVNLTPIQRASEKSVQYHLKQVIGRGSYGVVYKAINKHTDQVVAIKEVVYENDEELNDIMAEISLLKNLNHNNIVKYHGFIRKSYELYILLEYCANGSLRRLISRSSTGLSENESKTYVTQTLLGLKYLHGEGVIHRDIKAANILLSADNTVKLADFGVSTIVNSSALTLAGTLNWMAPEILGNRGASTLSDIWSLGATVVEMLTKNPPYHNLTDANIYYAVENDTYYPPSSFSEPLKDFLSKCFVKNMYKRPTADQLLKHVWINSTENVKVDKLNKFKEDFTDADYHWDADFQEEKLNISPSKFSLAAAPAAWAENNQELDLMPPTESQLLSQLKSSSKPLTDLHVLFSVCSLENIADTIIECLSRTTVDKRLITAFGSIFVYDTQHNHSRLRLKFIAMGGIPLIIKFEHLAKEFVIDYPQTLIECGIMYPPNFASLKTPKYILELVYRFYDLTSTAFWCRWCFKHLDISLLLNNIHERRAQSILLKLSSYAPWSFEKILPSLIDSKLKKKILISPQITYVVFKSINYMITTNDDKIHKSAIPSSSSLPLSSSPTRNSPVNSVQSPSRSPVHSLMATRPSSPMRHKSISNFPHLTISSKSRLLIELPEGFFTWLTSFFVDMAQIKDLSVLKYFTKLCYLTVHINSTFLNDLLDNDAFFAFIRNIDTIIPFIDDAKTAAFIWKQITAICVEMSLDMDQMSASLFSTAMNFIRKKNNTSISGLEIILNCLHFTLRNVNDDVAPTVGSSESHSVFLIKVNNDAAIELPIDQLVDLFYALNDDDVNLSKLISIFTKICSLPGFENLTINIIFHPNFYEKIVSFFDTYFNSLLIQIDLLKFIKLIFSKSLLKLYDYTGQPDPIKQTEPNRRNKATVFKLRAILVQITEFLNNNWNKDVPKRNSNQVGGDSVLICQLCEDIRSLSKKGSLQKVSSVTAAIGSSPTKDERSNLRSSKDKSDGFSVPITTFQT 99383BD871A4A726 99383BD871A4A726 amn1-tem1-1 1 5 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 5A tem1-amn1-1 1 6 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role bait bait c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 5A tem1-cdc15-1 1 7 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 5B, 5C Interaction is enhanced by deletion of AMN1 or BFA1. Interaction is inhibited by over-expression of AMN1. tem1-amn1-2 4 5 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 6A tem1-amn1-3 2 5 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 6 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 6B amn1-tem1-2 2 6 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro physical association physical association Fig 6B cdc15-tem1-1 3 7 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P38987:p.Asp135Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain D A Wed May 02 18:57:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp135ala' Tue Jun 30 17:15:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp135Ala' P38987:p.Thr34Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain T A Wed May 02 15:18:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr34ala' Tue Jun 30 16:45:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr34Ala' in vitro In vitro physical association physical association Fig 6D, 6G Interaction is inhibited by GST-AMN1. amn1-tem1-3 2 6 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P38987:p.Thr34Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain T A Wed May 02 19:56:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr34ala' Tue Jun 30 19:02:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr34Ala' P38987:p.Asp135Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain D A Wed May 02 18:49:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp135ala' Tue Jun 30 16:05:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp135Ala' in vitro In vitro physical association physical association Fig 6E, 6H Interaction is inhibited by His6-CDC15.