IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The signaling mechanism of Arabidopsis CRY1 involves direct interaction with COP1. The signaling mechanism of Arabidopsis CRY1 involves direct interaction with COP1. Plant Cell (1040-4651) 2001 imex curation Yang HQ., Tang RH., Cashmore AR. cashmore@sas.upenn.edu orchard Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yang HQ., Tang RH., Cashmore AR. cashmore@sas.upenn.edu Accepted 2009-AUG-10 by ORCHARD. Plant Cell (1040-4651) 2001 orchard Only protein-protein interactions imex curation The signaling mechanism of Arabidopsis CRY1 involves direct interaction with COP1. The signaling mechanism of Arabidopsis CRY1 involves direct interaction with COP1. Plant Cell (1040-4651) 2001 imex curation Yang HQ., Tang RH., Cashmore AR. cashmore@sas.upenn.edu orchard Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Yang HQ., Tang RH., Cashmore AR. cashmore@sas.upenn.edu Accepted 2009-AUG-10 by ORCHARD. Plant Cell (1040-4651) 2001 orchard Only protein-protein interactions imex curation The signaling mechanism of Arabidopsis CRY1 involves direct interaction with COP1. The signaling mechanism of Arabidopsis CRY1 involves direct interaction with COP1. Plant Cell (1040-4651) 2001 imex curation Yang HQ., Tang RH., Cashmore AR. cashmore@sas.upenn.edu orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yang HQ., Tang RH., Cashmore AR. cashmore@sas.upenn.edu Accepted 2009-AUG-10 by ORCHARD. Plant Cell (1040-4651) 2001 orchard Only protein-protein interactions imex curation cop1_arath E3 ubiquitin-protein ligase COP1 COP1 Constitutive photomorphogenesis protein 1 RING-type E3 ubiquitin transferase COP1 T21L14.11 At2g32950 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEISTDPVVPAVKPDPRTSSVGEGANRHENDDGGSGGSEIGAPDLDKDLLCPICMQIIKDAFLTACGHSFCYMCIITHLRNKSDCPCCSQHLTNNQLYPNFLLDKLLKKTSARHVSKTASPLDQFREALQRGCDVSIKEVDNLLTLLAERKRKMEQEEAERNMQILLDFLHCLRKQKVDELNEVQTDLQYIKEDINAVERHRIDLYRARDRYSVKLRMLGDDPSTRNAWPHEKNQIGFNSNSLSIRGGNFVGNYQNKKVEGKAQGSSHGLPKKDALSGSDSQSLNQSTVSMARKKRIHAQFNDLQECYLQKRRQLADQPNSKQENDKSVVRREGYSNGLADFQSVLTTFTRYSRLRVIAEIRHGDIFHSANIVSSIEFDRDDELFATAGVSRCIKVFDFSSVVNEPADMQCPIVEMSTRSKLSCLSWNKHEKNHIASSDYEGIVTVWDVTTRQSLMEYEEHEKRAWSVDFSRTEPSMLVSGSDDCKVKVWCTRQEASVINIDMKANICCVKYNPGSSNYIAVGSADHHIHYYDLRNISQPLHVFSGHKKAVSYVKFLSNNELASASTDSTLRLWDVKDNLPVRTFRGHTNEKNFVGLTVNSEYLACGSETNEVYVYHKEITRPVTSHRFGSPDMDDAEEEAGSYFISAVCWKSDSPTMLTANSQGTIKVLVLAA DIP protein P43254 original sequence version: 138 DIP protein P43254 original sequence version: 113 DIP protein P43254 original sequence version: 114 E6D9A1B702EAE8F0 cry1_arath Cryptochrome-1 CRY1 HY4 At4g08920 T3H13.5 T3H13.14 BLU1 Blue light photoreceptor Protein OUT OF PHASE 2 Protein BLUE LIGHT UNINHIBITED 1 Protein ELONGATED HYPOCOTYL 4 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSGSVSGCGSGGCSIVWFRRDLRVEDNPALAAAVRAGPVIALFVWAPEEEGHYHPGRVSRWWLKNSLAQLDSSLRSLGTCLITKRSTDSVASLLDVVKSTGASQIFFNHLYDPLSLVRDHRAKDVLTAQGIAVRSFNADLLYEPWEVTDELGRPFSMFAAFWERCLSMPYDPESPLLPPKKIISGDVSKCVADPLVFEDDSEKGSNALLARAWSPGWSNGDKALTTFINGPLLEYSKNRRKADSATTSFLSPHLHFGEVSVRKVFHLVRIKQVAWANEGNEAGEESVNLFLKSIGLREYSRYISFNHPYSHERPLLGHLKFFPWAVDENYFKAWRQGRTGYPLVDAGMRELWATGWLHDRIRVVVSSFFVKVLQLPWRWGMKYFWDTLLDADLESDALGWQYITGTLPDSREFDRIDNPQFEGYKFDPNGEYVRRWLPELSRLPTDWIHHPWNAPESVLQAAGIELGSNYPLPIVGLDEAKARLHEALSQMWQLEAASRAAIENGSEEGLGDSAEVEEAPIEFPRDITMEETEPTRLNPNRRYEDQMVPSITSSLIRPEEDEESSLNLRNSVGDSRAEVPRNMVNTNQAQQRRAEPASNQVTAMIPEFNIRIVAESTEDSTAESSSSGRRERSGGIVPEWSPGYSEQFPSEENGIGGGSTTSSYLQNHHEILNWRRLSQTG 372A7E6DDC2AC076 phyb_arath Phytochrome B PHYB HY3 OOP1 Protein LONG HYPOCOTYL 3 Protein OUT OF PHASE 1 MSF3.17 At2g18790 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSLRPRSNTESMSKAIQQYTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAYLSRIQRGGYIQPFGCMIAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDVRSLFTSSSSILLERAFVAREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTEDPALSIAGAVQSQKLAVRAISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHEDEHGEVVAESKRDDLEPYIGLHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRLTQSMCLVGSTLRAPHGCHSQYMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGLVVCHHTSSRCIPFPLRYACEFLMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRDSPAGIVTQSPSIMDLVKCDGAAFLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLSTDSLGDAGYPGAAALGDAVCGMAVAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWETAEMDAIHSLQLILRDSFKESEAAMNSKVVDGVVQPCRDMAGEQGIDELGAVAREMVRLIETATVPIFAVDAGGCINGWNAKIAELTGLSVEEAMGKSLVSDLIYKENEATVNKLLSRALRGDEEKNVEVKLKTFSPELQGKAVFVVVNACSSKDYLNNIVGVCFVGQDVTSQKIVMDKFINIQGDYKAIVHSPNPLIPPIFAADENTCCLEWNMAMEKLTGWSRSEVIGKMIVGEVFGSCCMLKGPDALTKFMIVLHNAIGGQDTDKFPFPFFDRNGKFVQALLTANKRVSLEGKVIGAFCFLQIPSPELQQALAVQRRQDTECFTKAKELAYICQVIKNPLSGMRFANSLLEATDLNEDQKQLLETSVSCEKQISRIVGDMDLESIEDGSFVLKREEFFLGSVINAIVSQAMFLLRDRGLQLIRDIPEEIKSIEVFGDQIRIQQLLAEFLLSIIRYAPSQEWVEIHLSQLSKQMADGFAAIRTEFRMACPGEGLPPELVRDMFHSSRWTSPEGLGLSVCRKILKLMNGEVQYIRESERSYFLIILELPVPRKRPLSTASGSGDMMLMMPY DIP protein P14713 original sequence version: 107 DIP protein P14713 original sequence version: 131 7B5348CB1091B813 cry1-cop1-1 3 4 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain P43254:p.Lys422Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K E Wed May 02 18:01:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys422glu' Tue Jun 30 17:59:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys422Glu' P43254:p.Arg465Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R E Wed May 02 14:33:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg465glu' Tue Jun 30 18:15:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg465Glu' P43254:p.Trp467Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain W A Wed May 02 16:40:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp467ala' Tue Jun 30 17:16:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp467Ala' P43254:p.Gly524Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G E Wed May 02 15:42:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly524glu' Tue Jun 30 18:41:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly524Glu' P43254:p.Lys550Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K E Wed May 02 15:58:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys550glu' Tue Jun 30 16:59:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys550Glu' P43254:p.Glu592Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E R Wed May 02 15:44:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu592arg' Tue Jun 30 18:22:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu592Arg' 5 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q43125:p.Pro549Leu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain P L Wed May 02 17:00:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro549leu' Tue Jun 30 20:21:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro549Leu' Q43125:p.Arg576Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R K Wed May 02 16:58:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg576lys' Tue Jun 30 17:11:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg576Lys' Q43125:p.Glu515Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 18:46:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu515lys' Tue Jun 30 17:46:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu515Lys' Q43125:p.Glu531Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain E K Wed May 02 15:12:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu531lys' Tue Jun 30 17:48:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu531Lys' Q43125:p.Glu559Lys mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain E K Wed May 02 16:23:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu559lys' Tue Jun 30 18:14:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu559Lys' Q43125:p.Arg581Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R K Wed May 02 16:57:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg581lys' Tue Jun 30 18:23:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg581Lys' Q43125:p.Arg611Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R K Wed May 02 16:33:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg611lys' Tue Jun 30 17:27:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg611Lys' 17 9 physical association physical association Figure 1 Figure 4 Arabidopsis CCT1 Interacts with COP1 in Yeast. The authors concluded that the "interaction between Arabidopsis CRY1 and COP1 in yeast cells was light independent". phyb-cop1-2 3 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 3 The phyB C-Terminal Domain Interacts with COP1. cry1-cop1-2 2 5 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region GAL4 activation domain tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 4 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Figure 6 In Vitro Binding of Arabidopsis CRY1 to COP1 cry1-cop1-3 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 6D The ability of the COP1 protein to bind to CRY1 was determined by probing the same blot with antibody against CCT1. As shown in Figure 6D, CRY1 was bound to GFP�COP1 in both the dark- and light-grown Arabidopsis samples (lanes 2 and 4)