UniProt UniProt http://www.uniprot.org Prospore membrane formation linked to the leading edge protein (LEP) coat assembly. Prospore membrane formation linked to the leading edge protein (LEP) coat assembly. EMBO J. (0261-4189) 2001 imex curation Moreno-Borchart AC., Strasser K., Finkbeiner MG., Shevchenko A., Shevchenko A., Knop M. michael.knop@embl-hiedelberg.de 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Moreno-Borchart AC., Strasser K., Finkbeiner MG., Shevchenko A., Shevchenko A., Knop M. michael.knop@embl-hiedelberg.de Accepted 2009-FEB-19 by JYOTI. 03-Mar-2009: Contacted by JYOTI. EMBO J. (0261-4189) 2001 jyoti Only protein-protein interactions imex curation Prospore membrane formation linked to the leading edge protein (LEP) coat assembly. Prospore membrane formation linked to the leading edge protein (LEP) coat assembly. EMBO J. (0261-4189) 2001 imex curation Moreno-Borchart AC., Strasser K., Finkbeiner MG., Shevchenko A., Shevchenko A., Knop M. michael.knop@embl-hiedelberg.de 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Moreno-Borchart AC., Strasser K., Finkbeiner MG., Shevchenko A., Shevchenko A., Knop M. michael.knop@embl-hiedelberg.de Accepted 2009-FEB-19 by JYOTI. 03-Mar-2009: Contacted by JYOTI. EMBO J. (0261-4189) 2001 jyoti Only protein-protein interactions imex curation Prospore membrane formation linked to the leading edge protein (LEP) coat assembly. Prospore membrane formation linked to the leading edge protein (LEP) coat assembly. EMBO J. (0261-4189) 2001 imex curation Moreno-Borchart AC., Strasser K., Finkbeiner MG., Shevchenko A., Shevchenko A., Knop M. michael.knop@embl-hiedelberg.de 03-Mar-2009: Contacted by JYOTI. 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down ms participant Identification by mass spectrometry ms participant Moreno-Borchart AC., Strasser K., Finkbeiner MG., Shevchenko A., Shevchenko A., Knop M. michael.knop@embl-hiedelberg.de Accepted 2009-FEB-19 by JYOTI. 03-Mar-2009: Contacted by JYOTI. EMBO J. (0261-4189) 2001 jyoti Only protein-protein interactions imex curation sso1_yeast Protein SSO1 SSO1 P1405 YPL232W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSYNNPYQLETPFEESYELDEGSSAIGAEGHDFVGFMNKISQINRDLDKYDHTINQVDSLHKRLLTEVNEEQASHLRHSLDNFVAQATDLQFKLKNEIKSAQRDGIHDTNKQAQAENSRQRFLKLIQDYRIVDSNYKEENKEQAKRQYMIIQPEATEDEVEAAISDVGGQQIFSQALLNANRRGEAKTALAEVQARHQELLKLEKSMAELTQLFNDMEELVIEQQENVDVIDKNVEDAQLDVEQGVGHTDKAVKSARKARKNKIRCWLIVFAIIVVVVVVVVVPAVVKTR 6E4C6D35C3CD9D94 6E4C6D35C3CD9D94 mpc54_yeast Meiotic plaque component protein 54 MPC54 YOR177C protein protein yeast Saccharomyces cerevisiae Baker's yeast MPEDTSYSNSFEDYYNNSHAISPYKDSFYKEMTPSKPNVRFGDDDVNIFDQRKKVNEINKNNTVKRSIPSSISTTITPNKSSLKSPRGKRASKNSFDNETKLESKNETLKEVNDAVNRCYALCNIPTKHVSINSISDLAQTFETLAVGITHETNRKAECERSKNAIDSLYYHEQLEKKELNEKSLQMAIDHLLKVTKQNLRQADDGNKLKETEALKSFIEEIEEVDDNKISINSLQQQLLEEKTANNILRRDYYKLQERGRRLCHEFQELQDDYSKQMKQKEYEVQKLKNEIKVLLNMNDNLKAEKAHYSQKEKQYFQKYTYIEKYMNHVKEEYNRKEDECKKLNFIIDKSMKKIEHLERSLQTQFTAQNSFSTAMIQEEGPKDAHLKDRYHKVKEFMEQKLQTSKINDPSCSEAEALDNVLCLIESSMKTLDKNSKCYPIATKKCIKYVTDSPRLKENEHVTN 74C614223B385CAC 74C614223B385CAC nud1_yeast Protein NUD1 YOR373W NUD1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDMDTQEAELSSQLENLTINSPRKLRSNAHSNSGKVFKEYESNHDFQDSNFTSQVVEPAISDSVKKPPTMTVLNNYSTVHQKVPSGFSGTTATSHQEAQWKQYFPGIGSGGGTNFGGAVGTANKVPESDLIVSDLVKDLSGVLETNTFKRHLDMKNKTTTMQTHENHDTISISHSKDFFNAEKVSSSFSDDSDSGPAAEAHDVFDGILQKQKSNYLVGSYPSNSNNKNNNNNNNNNNNNSININNKDNARTKEEDEEDTSNSFEFSSSSSMSSSQTQSGRKSKVLKKPPLNTISPGQLGYQFNHTHGAWDPPLNQGLDVSSSHSLDNTSSNQSQFATMVPTGDNHTNGKAPSILDKKAYELTSTKPGDVGYRQKKIQEEENLANSDDTPLDTPKFNDLFTKNGTRAKVKGQMRTSRSISNSNLLEAHKKLKTFPAERVEDITSISEVNTSFNETEKQLISILTSKLSGSPSYDSDWEKILKVDLSRGKLKNMFGMQRLLPNVLVLNLSDNEMNTLEGIPSNVVQLFCSNNKITSAHCSLAGFHDLECLDLSYNLLNTSLKFLSLCHHLQEVNLSYNSIQSLEGIGSSRMKKLNLSNNEINGIIDFEQLILTNNSVVGGWLTVEVLDLSNNNIIGVRNINCLPRLKVLNLNGNPLVSIVESSKMENGTLRALSIKNTGGALSKLQNYKLDDQFTFPYQNLKILKLDGFAQLSKWQKWPATLQILEINGGLASSLPRFSSLKSTNLYSLTIANVRDFTHLPVDLSKELPFLQELHLPGNNLQNAHKLTKTLPRQSVKFLDLRNNPITTPRHDRASTSLHYRQLLQLAGLCQQQCPALATLWLDDTPAPTATNL CB9F0408633C1315 sso2_yeast Protein SSO2 SSO2 YM8010.13C YMR183C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNANPYENNNPYAENYEMQEDLNNAPTGHSDGSDDFVAFMNKINSINANLSRYENIINQIDAQHKDLLTQVSEEQEMELRRSLDDYISQATDLQYQLKADIKDAQRDGLHDSNKQAQAENCRQKFLKLIQDYRIIDSNYKEESKEQAKRQYTIIQPEATDEEVEAAINDVNGQQIFSQALLNANRRGEAKTALAEVQARHQELLKLEKTMAELTQLFNDMEELVIEQQENVDVIDKNVEDAQQDVEQGVGHTNKAVKSARKARKNKIRCLIICFIIFAIVVVVVVVPSVVETRK 454CF2B45FFE8E22 mpc70_yeast Sporulation-specific protein 21 Meiotic plaque component protein 70 SPO21 MPC70 YOL091W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDNILKASNMEGTSTMTVTSRSSEDSSCISNHEQDTDTHKDGDTSGLENSKISKRKWMKEFFKLSKSPASKSSRSIGSMKSNQSLVSMKSSDDGNSYKNDYSSICGNSLPSAGLSRSNSVKELKLDSTGSQRSKNNVAMLARSSTTSQTTCSSSSSSSSYNSIKGNENDILLQNNNHFRHNKEIPQSKGSSNINTASIMSQYNVDTQATAIMSDMQKQYDSQQMTSPFVNEDLHFDPNGEVSHVIKAIFKEIGYKYDDFSDIPVFQLMQEMYQLVKKNSSARRTKITDYASKLKEKEAQLKSQNDKILKLETTNKAYKTKYKEVSLENKKIKEAFKELDNESYNHDEELLKKYKYTRETLDRVNREQQLIIDQNEFLKKSVNELQNEVNATNFKFSLFKEKYAKLADSITELNTSTKKREALGENLTFECNELKEICLKYKKNIENISNTNKNLQNSFKNERKKVLDLRNERNLLKKEILLIECHGSYSLLLVSNILTCYRFLLPSDTIIETESLIKELLNMNNSLSNHVSSSDEPPAEYSKRLELKCVEFEEKLLYFYQELVTKKIIDVIYKCFINYYKKSRQTDQKSNQNSSTPYKQSQRQVPHSIK E216B0C9B865B412 E216B0C9B865B412 ady3_yeast Accumulates dyads protein 3 YDL239C ADY3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNHWLAFLNKPESLKEQNSDCDQQGEMRHVTDGTLTKSPESKPFRERRSQTWIDSEVPTSTEKSNVQESISSDIISKLSNRRSRRNRSESWAGSEASSPSGNISTLENATEKNTLKSPNKFLQRGGLPTVGIGSQALSPAGKPSTLGNVSPGKFTTYKVHNSIEVNRFSSTPTKLLTNPHKVAAISNDEHYVVSNESLEENIEVAHLENVFRSSKTPDEEQSEYMKLGEIRLSSSSYGGSISKENSLPKVLDELQSQNEEIKALRQKLEEKDDRIQELEELNSMNDAKLQRIEDLQKEFHNERKAASKRLNIVQDRFRKEIKKIREEKITDFQNKNASKKEKNEVTSAKTKCKAFSQRNILVSELYRKQKQILNLQQENDKFLKDINESNNSIVKLRSEVEILKSNLQLSQDENKKLHDNGSFYEKRLNDVYSYMQNLSLFEKDLGKFILEEMKCGHSPSMFQNGFAKLYPDFQDIKNLENMEQYKQLKGKIELLEKNDRIRLEKIISVFKLINERLHFMQQQHSHKIKYLQKEALTKEQQFRLEKRRWHDILNLKEENFQKLKSELKEKLILSEKIQKNAEDKLNDYMNEHQEIVEKLQNQALIASRWSTQIQESENTHKKITDELAGKQSEILKLEETILSLKEDVFQEKLNLKKLYGDPSTELNFETVGKSFPHITKEKYDSLGLDILTDLTYVQSQNLIKNLLIVLDIPLKTFLKIVPTIVIQLRCELTLLTKFANDLNLKVFGKQLDFKSRRKVAMNEFLNNHDIAEVKHPLEYDLQALFKYFFS 46F17474F30FD3D9 46F17474F30FD3D9 cnm67_yeast Chaotic nuclear migration protein 67 CNM67 N1264 YNL225C protein protein yeast Saccharomyces cerevisiae Baker's yeast MTDFDLMNFPFHERLDSPVSENGEIKDGEPIPQNWLNENHVGKSILPLFVNPEDVINCNFSNARDSYEENKSPSMDQMNYARNTSYQESPGLQERPKNEKDKSPIGTDVHKKDVPNFIHSTPRENSSKHFTRANEQASAQPTDEHTSPDISIEDCNGAKIFLQNSLSKEDFRMLENVILGYQKKVIELGRDNLRQEERANSLQKELEAATKSNDKTLDNKKKIEEQTVLIENLTKDLSLNKEMLEKANDTIQTKHTALLSLTDSLRKAELFEIPIGILFFDLYDSEENSSKLDHILQEKYPNIKGFLCASQQEELSRISQRFKNAKAEAEDLRNELENKKIEIQTMREKNNTLIGTNKTLSKQNKILCDKFDKLTIDEKEILKGCNEEIKIKLERLNERLGSWEKSKEKYETSLKDKEKMLADAEKKTNTLSKELDNLRSRFGNLEGNTSERITIKNILQSRPDISAEECNFLMVEQIDSANLTTLQNTVKEIVLAVGIPYPKLRRKIPLLAIKLKYENIMLSNFAQRLHRQVYSQEMNLKKFTDQAYYDFMSTRRMDSIDHHLERCLDHLYDHILEKMVK C4DE7631A70E695A C4DE7631A70E695A don1_yeast Donuts protein 1 DON1 D9954.4 YDR273W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGKKNRKGKENNAAKTSFLKVENIKNTNSGLELPSQDYTNVEEKESSPKTDFPLITKEHVNTKTDSNILDYPTIGDLVSSVEKLCVLKELKIAFPEVDDTLIKAILIASQGVLEPAFNSLLYYSSPEENTDFALPMKPISVEDYSKINVSEILQREIFDDIEDEFSGQGINGSMVISKIESELSSLAEHIGNISTPGSNREVAESTRNVAVAEGHNTILSNEDSILKGKEKGKEEEKEKGEEKGVNSLKGAAVKVVAKSLKNNRIPVTVKRNEPSNNLFDVLNCDESEEEEEQDVETNTSNQERKNQGGNTEVPEAQRDSADRLPAKDDGGYKSAFGTDSCGLFAADAKDEKKQVHPSRQELSFT 6E13542050862709 6E13542050862709 ssp1_yeast Sporulation-specific protein 1 SSP1 YHR184W protein protein yeast Saccharomyces cerevisiae Baker's yeast MRSSGTYENDPSGEITSTSPKQSKQKKPTKFRERMRRWLQNGKNNNHQGEEDVPEIFNKNFYPQTGMTAFNNNDNGEVQDVTNNFFLPSEDESGPVQSSVKTFLTGNNDEDSNFQQNQNPKQKSELPKSPYRQKPTQEIALLKDLFVTNKYDDPYLNSSTRFGNITSTFPSSLSLRTVTLQTIKKRIDCISAKKKEVWKTEEKFLKDILMWLQSSNFEDPDTISLIHEIEKIFEEDIHFEQNVSDCLKEISNNFEYICMRETQLINEGNILKNDLKKYAKAREHKGEKHEDTEVLREKVISSQKSFDVTKRHYKHAISITTRQLFMNLAFEYYENCSDMKDISRKYLQESLSTLQTIDTLSFSEELEKIRKRRFDKFWAKTNPDPTNNIQKFVNMRTGVAGFNDSLMNHLYGKLSFGVAPVEEELQNSQPEHTDVPENVWNEVLSDYNSMDGNPITSNKFLSAKELEPDQLVELLAQEKEEKEAKNISSSTAEVPSISQPEIKKENLESNDSLILRSTKRNVNVNAASLRNLSIKKTQVKPESASEESKVLAAALNDAKQNLDENVWRTPI A199EB4159771A03 A199EB4159771A03 mpc54-ady3-1 3 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait c-terminus protein a tag protein a tag protein A protein A c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 1A mpc54-ady3-2 1 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-2133892]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 465-465, sequence length 464 [EBI-2133892]150-465 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1B ady3-mpc54-1 1 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-2133907]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 465-465, sequence length 464 [EBI-2133907]150-465 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1B mpc70-ady3-1 1 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1B ady3-mpc70-1 1 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1B ady3-nud1-1 1 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1B ady3-cnm67-1 1 10 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1B ady3-ssp1-1 1 9 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association Fig 1B ady3-ady3-1 1 9 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Fig 1C don1-don1-1 1 11 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Fig 1C ssp1-ssp1-1 1 12 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association Fig 1C ady3-don1-2 2 9 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig 3A ssp1-don1-1 2 11 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 12 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 3C, 3D sso1-don1-1 2 4 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig 6 The antibody used recognizes both SSO1 and SSO2. The interaction requires SSP1.