IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Detection of in vivo protein interactions between Snf1-related kinase subunits with intron-tagged epitope-labelling in plants cells. Detection of in vivo protein interactions between Snf1-related kinase subunits with intron-tagged epitope-labelling in plants cells. Nucleic Acids Res. (0305-1048) 2001 imex curation Ferrando A., Koncz-Kalman Z., Farras R., Tiburcio A., Schell J., Koncz C. koncz@mpiz-koeln.mpg.de orchard Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ferrando A., Koncz-Kalman Z., Farras R., Tiburcio A., Schell J., Koncz C. koncz@mpiz-koeln.mpg.de Accepted 2009-AUG-23 by ORCHARD. Nucleic Acids Res. (0305-1048) 2001 orchard Only protein-protein interactions imex curation Detection of in vivo protein interactions between Snf1-related kinase subunits with intron-tagged epitope-labelling in plants cells. Detection of in vivo protein interactions between Snf1-related kinase subunits with intron-tagged epitope-labelling in plants cells. Nucleic Acids Res. (0305-1048) 2001 imex curation Ferrando A., Koncz-Kalman Z., Farras R., Tiburcio A., Schell J., Koncz C. koncz@mpiz-koeln.mpg.de orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ferrando A., Koncz-Kalman Z., Farras R., Tiburcio A., Schell J., Koncz C. koncz@mpiz-koeln.mpg.de Accepted 2009-AUG-23 by ORCHARD. Nucleic Acids Res. (0305-1048) 2001 orchard Only protein-protein interactions imex curation kinb2_arath SNF1-related protein kinase regulatory subunit beta-2 KINB2 dl4210w FCAALL.163 At4g16360 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGNVNAREEANSNNASAVEDEDAEICSREAMSAASDGNHVAPPELMGQSPPHSPRATQSPLMFAPQVPVLPLQRPDEIHIPNPSWMQSPSSLYEEASNEQGIPTMITWCHGGKEIAVEGSWDNWKTRSRLQRSGKDFTIMKVLPSGVYEYRFIVDGQWRHAPELPLARDDAGNTFNILDLQDYVPEDIQSISGFEPPQSPENSYSNLLLGAEDYSKEPPVVPPHLQMTLLNLPAANPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAKYVTVVLYKSLQR FF76EAD739E0CEBB kin10_arath SNF1-related protein kinase catalytic subunit alpha KIN10 KIN10 AK21 SKIN10 SNR2 AKIN alpha-2 SNRK1.1 T4P13.22 At3g01090 AKIN10 SNF1-related kinase 1.1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDGSGTGSRSGVESILPNYKLGRTLGIGSFGRVKIAEHALTGHKVAIKILNRRKIKNMEMEEKVRREIKILRLFMHPHIIRLYEVIETPTDIYLVMEYVNSGELFDYIVEKGRLQEDEARNFFQQIISGVEYCHRNMVVHRDLKPENLLLDSKCNVKIADFGLSNIMRDGHFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYALLCGTLPFDDENIPNLFKKIKGGIYTLPSHLSPGARDLIPRMLVVDPMKRVTIPEIRQHPWFQAHLPRYLAVPPPDTVQQAKKIDEEILQEVINMGFDRNHLIESLRNRTQNDGTVTYYLILDNRFRASSGYLGAEFQETMEGTPRMHPAESVASPVSHRLPGLMEYQGVGLRSQYPVERKWALGLQSRAHPREIMTEVLKALQDLNVCWKKIGHYNMKCRWVPNSSADGMLSNSMHDNNYFGDESSIIENEAAVKSPNVVKFEIQLYKTRDDKYLLDLQRVQGPQFLFLDLCAAFLAQLRVL 5A18655A0AA506DF kin11_arath SNF1-related protein kinase catalytic subunit alpha KIN11 KIN11 AKIN alpha-1 AKIN11 SNF1-related kinase 1.2 SNR1 SNRK1.2 MXE2.16 At3g29160 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDHSSNRFGNNGVESILPNYKLGKTLGIGSFGKVKIAEHVVTGHKVAIKILNRRKIKNMEMEEKVRREIKILRLFMHPHIIRQYEVIETTSDIYVVMEYVKSGELFDYIVEKGRLQEDEARNFFQQIISGVEYCHRNMVVHRDLKPENLLLDSRCNIKIADFGLSNVMRDGHFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYALLCGTLPFDDENIPNLFKKIKGGIYTLPSHLSSEARDLIPRMLIVDPVKRITIPEIRQHRWFQTHLPRYLAVSPPDTVEQAKKINEEIVQEVVNMGFDRNQVLESLRNRTQNDATVTYYLLLDNRFRVPSGYLESEFQETTDSGSNPMRTPEAGASPVGHWIPAHVDHYGLGARSQVPVDRKWALGLQSHAHPREIMNEVLKALQELNVCWKKIGHYNMKCRWVPGLADGQNTMVNNQLHFRDESSIIEDDCAMTSPTVIKFELQLYKAREEKYLLDIQRVNGPQFLFLDLCAAFLTELRVI 4FBEF55D3EC60F87 akin10-kinb2 2 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association Figure 4 A cDNA encoding AKINβ2 in fusion with the Gal4 activation domain was isolated from a cDNA library made in the yeast two-hybrid vector pACT2 (16) using a specific probe obtained by PCR amplification of the last exon. This cDNA clone was used as prey in the yeast strain Y187 to test for interaction of AKINβ2 with the Arabidopsis SnRK1 α-subunits AKIN10 and AKIN11 used as baits in two-hybrid assays. akin11-kinb2-1 2 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Figure 4 A cDNA encoding AKINβ2 in fusion with the Gal4 activation domain was isolated from a cDNA library made in the yeast two-hybrid vector pACT2 (16) using a specific probe obtained by PCR amplification of the last exon. This cDNA clone was used as prey in the yeast strain Y187 to test for interaction of AKINβ2 with the Arabidopsis SnRK1 α-subunits AKIN10 and AKIN11 used as baits in two-hybrid assays. akin10-kinb2-1 1 3 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role bait bait n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 6 AKINβ2 is detected in different protein complexes with SnRK α-subunits AKIN10 and AKIN11 in vivo. akin10-kinb2-2 1 4 unspecified role unspecified role prey prey n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 6 AKINβ2 is detected in different protein complexes with SnRK α-subunits AKIN10 and AKIN11 in vivo. akin11-kinb2-2 1 3 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role bait bait c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Figure 6 akin11-kinb2-3 1 5 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 6