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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>MINT</shortLabel>
        <fullName>MINT, Dpt of Biology, University of Rome Tor Vergata</fullName>
        <alias type="synonym" typeAc="MI:1041">MINT</alias>
      </names>
      <bibref>
        <xref>
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      </bibref>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0471" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="mint" dbAc="MI:0471" id="MINT-80017" refType="identity" refTypeAc="MI:0356"/>
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      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it/mint</attribute>
        <attribute name="url" nameAc="MI:0614">url&amp;gt;http://mint.bio.uniroma2.it/mint/</attribute>
        <attribute name="url" nameAc="MI:0614">https://mint.bio.uniroma2.it</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">MINT-[0-9]+</attribute>
        <attribute name="search-url" nameAc="MI:0615">https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac}</attribute>
        <attribute name="definition">The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules.</attribute>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>The effector enzyme regulates the duration of G protein signaling in vertebrate photoreceptors by increasing the affinity between transducin and RGS protein.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="10973941" refType="primary-reference" refTypeAc="MI:0358"/>
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            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-7381599" refType="identity" refTypeAc="MI:0356"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">The effector enzyme regulates the duration of G protein signaling in vertebrate photoreceptors by increasing the affinity between transducin and RGS protein.</attribute>
            <attribute name="journal" nameAc="MI:0885">J. Biol. Chem. (0021-9258)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2000</attribute>
            <attribute name="author-list" nameAc="MI:0636">Skiba NP., Hopp JA., Arshavsky VY.</attribute>
            <attribute name="remark-internal">suggested by aryamontry</attribute>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="mint" dbAc="MI:0471" id="MINT-6825844" refType="identity" refTypeAc="MI:0356"/>
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        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>pull down</shortLabel>
            <fullName>pull down</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0096" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Skiba NP., Hopp JA., Arshavsky VY.</attribute>
          <attribute name="remark-internal">suggested by aryamontry</attribute>
          <attribute name="journal" nameAc="MI:0885">J. Biol. Chem. (0021-9258)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2000</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>gnat1_bovin</shortLabel>
          <fullName>Guanine nucleotide-binding protein G(t) subunit alpha-1</fullName>
          <alias type="gene name" typeAc="MI:0301">GNAT1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Transducin alpha-1 chain</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P04695" version="SP_82" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSBTAP00000023990" version="SP_197" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="UNK-6021598" refType="intact-secondary"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-7052221" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSBTAG00000018020" version="SP_197" refType="gene"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSBTAT00000023990" version="SP_197" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0000035" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0001664" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0001917" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0001973" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0003924" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005525" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005834" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0007188" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0007193" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0007602" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0007603" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0008283" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0009416" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0019003" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0019901" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0031683" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0032391" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042462" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042622" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0046872" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0050908" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0050917" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0051344" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0060041" version="SP_197"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0071257" version="SP_197"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:0097648" version="SP_197"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR001019" version="SP_197"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR001408" version="SP_197"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011025" version="SP_197"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR027417" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3V00" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4BEY" version="SP_197"/>
          <secondaryRef db="mint" dbAc="MI:0471" id="P04695" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4J4Q" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6OY9" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1AQG" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="6OYA" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="7JSN" version="SP_197"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-BTA-2514859" version="SP_197"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-BTA-418594" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1FQJ" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1FQK" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1GOT" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1LVZ" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1TAD" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1TAG" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1TND" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2X72" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3DQB" version="SP_197"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3PQR" version="SP_197"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="XP_010815966.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_851365.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-29226N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9913">
          <names>
            <shortLabel>bovin</shortLabel>
            <fullName>Bos taurus (Bovine)</fullName>
            <alias type="synonym" typeAc="MI:1041">Bovine</alias>
          </names>
        </organism>
        <sequence>MGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFDRASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFSEKIKKAHLSICFPDYNGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein P04695 original sequence version: 74</attribute>
          <attribute name="function" nameAc="MI:0613">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase</attribute>
          <attribute name="comment" nameAc="MI:0612">mint</attribute>
          <attribute name="crc64">36F0B4630EFBD7DF</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>rgs9_bovin</shortLabel>
          <fullName>Regulator of G-protein signaling 9</fullName>
          <alias type="gene name" typeAc="MI:0301">RGS9</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="O46469" version="SP_64" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="UNK-6013739" refType="intact-secondary"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-6949890" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0007186" version="SP_156"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0007601" version="SP_156"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0009968" version="SP_156"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0035556" version="SP_156"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0097381" version="SP_156"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1FQI" version="SP_156"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1FQJ" version="SP_156"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1FQK" version="SP_156"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR040759"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR015898"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_776595.1"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR016137"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000591"/>
          <secondaryRef db="mint" dbAc="MI:0471" id="O46469"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036284"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036305"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036388"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036390"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9913">
          <names>
            <shortLabel>bovin</shortLabel>
            <fullName>Bos taurus (Bovine)</fullName>
            <alias type="synonym" typeAc="MI:1041">Bovine</alias>
          </names>
        </organism>
        <sequence>MTIRHQGQQYRPRMAFLRKIEALVKDMQDPDTGVRVQNQKVKVVSIPHAMTGSDVLQWISQRLWISGLEAQNLGNFIVKYGYIYPLQDPRNLTLKPDSSLYRFQTPYFWPTQQWPAEDVDYAIYLAKRNIKKKGILEEYEKENYNFLNKKINYKWDFVIMQAREQYRAGKERNKVDRCALDCQEKAYWLVHRCPPGANNVLDYGLDRVTNPNEDQKQTVVSVRKEIMYYRQALMRSTVKSSVSLGGIVKYSEQFSSNDAIMSGCLPSNPWITDDTQFWDLNAKLVDIPTKMRVERWAFNFSELIRDPKGRQSFQHFLRKEFSGENLGFWEACEDLKYGDQSKVKEKAEEIYKLFLAPGARRWINIDGKTMDITVKGLKHPHRYVLDAAQTHIYMLMKKDSYARYLKSPIYKEMLAKAIEPQGTTRKSSSLPFMRRHLRSSPSPVILRQLEEEAKAREAATTVDITQVMSKLDRRSQLRKEPPPK</sequence>
        <attributeList>
          <attribute name="function" nameAc="MI:0613">Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction</attribute>
          <attribute name="comment" nameAc="MI:0612">mint</attribute>
          <attribute name="crc64">3D8C61CEC1E22949</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4">
        <names>
          <shortLabel>gnat1-rgs9</shortLabel>
        </names>
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          <primaryRef db="mint" dbAc="MI:0471" id="MINT-6825846" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-7381613" refType="identity" refTypeAc="MI:0356"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <names>
              <alias type="author assigned name" typeAc="MI:0345">RGS9</alias>
            </names>
            <xref>
              <primaryRef db="mint" dbAc="MI:0471" id="MINT-6825852" refType="identity" refTypeAc="MI:0356"/>
              <secondaryRef db="uniprotkb" dbAc="MI:0486" id="O46469" refType="source reference" refTypeAc="MI:0685"/>
            </xref>
            <interactorRef>3</interactorRef>
            <participantIdentificationMethodList>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>western blot</shortLabel>
                  <fullName>western blot</fullName>
                  <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
                  <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-973" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
                  <alias type="synonym" typeAc="MI:1041">predetermined</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
            </participantIdentificationMethodList>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>cell lysate</shortLabel>
                  <fullName>cell lysate</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0344" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537757" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
              <experimentalPreparation>
                <names>
                  <shortLabel>physiological level</shortLabel>
                  <fullName>physiological level</fullName>
                  <alias type="go synonym" typeAc="MI:0303">endogenous level</alias>
                  <alias type="go synonym" typeAc="MI:0303">endogenous</alias>
                  <alias type="synonym" typeAc="MI:1041">endogenous</alias>
                  <alias type="synonym" typeAc="MI:1041">endogenous level</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0222" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537793" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
          </participant>
          <participant id="6">
            <names>
              <alias type="author assigned name" typeAc="MI:0345">transducin</alias>
            </names>
            <xref>
              <primaryRef db="mint" dbAc="MI:0471" id="MINT-6825847" refType="identity" refTypeAc="MI:0356"/>
              <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P04695" refType="source reference" refTypeAc="MI:0685"/>
            </xref>
            <interactorRef>2</interactorRef>
            <participantIdentificationMethodList>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
                  <alias type="synonym" typeAc="MI:1041">predetermined</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
              <participantIdentificationMethod>
                <names>
                  <shortLabel>predetermined</shortLabel>
                  <fullName>predetermined participant</fullName>
                  <alias type="synonym" typeAc="MI:1041">predetermined</alias>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </participantIdentificationMethod>
            </participantIdentificationMethodList>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <featureList>
              <feature id="7">
                <names>
                  <shortLabel>his tagged</shortLabel>
                </names>
                <xref>
                  <primaryRef db="mint" dbAc="MI:0471" id="MINT-6825850" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-7381625" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>his tag</shortLabel>
                    <fullName>his tag</fullName>
                    <alias type="go synonym" typeAc="MI:0303">Hexa-His-tag</alias>
                    <alias type="go synonym" typeAc="MI:0303">6-His-tag</alias>
                    <alias type="go synonym" typeAc="MI:0303">Histidine-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">Hexa-His-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">Histidine-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">6-His-tag</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0521" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456516" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>undetermined</shortLabel>
                        <fullName>undetermined sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">undetermined</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <endStatus>
                      <names>
                        <shortLabel>undetermined</shortLabel>
                        <fullName>undetermined sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">undetermined</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">F4</attribute>
          <attribute name="comment" nameAc="MI:0612">mint</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>