IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3, and its expression is induced by abscisic acid. ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3, and its expression is induced by abscisic acid. Plant J. (0960-7412) 1998 Wang H., Qi Q., Schorr P., Cutler AJ., Crosby WL., Fowke LC. WangHK@em.agr.ca 17-April-2008: Contacted by JYOTI. A cDNA of Arabidopsis thaliana (ecotype Columbia) was used in this study. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wang H., Qi Q., Schorr P., Cutler AJ., Crosby WL., Fowke LC. There are 3 Cyclin D3 genes in Arabidopsis and the text does not specify which was used in this study. Therefore interactions involving this protein were not curated. WangHK@em.agr.ca Accepted 2008-MAR-20 by ORCHARD. 17-April-2008: Contacted by JYOTI. A cDNA of Arabidopsis thaliana (ecotype Columbia) was used in this study. Plant J. (0960-7412) 1998 orchard ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3, and its expression is induced by abscisic acid. ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3, and its expression is induced by abscisic acid. Plant J. (0960-7412) 1998 Wang H., Qi Q., Schorr P., Cutler AJ., Crosby WL., Fowke LC. WangHK@em.agr.ca 17-April-2008: Contacted by JYOTI. A cDNA of Arabidopsis thaliana (ecotype Columbia) was used in this study. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wang H., Qi Q., Schorr P., Cutler AJ., Crosby WL., Fowke LC. WangHK@em.agr.ca Accepted 2008-MAR-20 by ORCHARD. 17-April-2008: Contacted by JYOTI. A cDNA of Arabidopsis thaliana (ecotype Columbia) was used in this study. Plant J. (0960-7412) 1998 orchard ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3, and its expression is induced by abscisic acid. ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3, and its expression is induced by abscisic acid. Plant J. (0960-7412) 1998 Wang H., Qi Q., Schorr P., Cutler AJ., Crosby WL., Fowke LC. WangHK@em.agr.ca 17-April-2008: Contacted by JYOTI. A cDNA of Arabidopsis thaliana (ecotype Columbia) was used in this study. orchard in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wang H., Qi Q., Schorr P., Cutler AJ., Crosby WL., Fowke LC. There are 3 cyclin D3 genes in Arabidopsis and from the information provided in the text it was not possible to identify which was used in this study. WangHK@em.agr.ca Accepted 2008-MAR-20 by ORCHARD. 17-April-2008: Contacted by JYOTI. A cDNA of Arabidopsis thaliana (ecotype Columbia) was used in this study. Plant J. (0960-7412) 1998 orchard krp1_arath Cyclin-dependent kinase inhibitor 1 KRP1 ICK1 F26B6.8 At2g23430 KIP-related protein 1 Inhibitor/interactor of CDK protein 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVRKYRKAKGIVEAGVSSTYMQLRSRRIVYVRSEKSSSVSVVGDNGVSSSCSGSNEYKKKELIHLEEEDKDGDTETSTYRRGTKRKLFENLREEEKEELSKSMENYSSEFESAVKESLDCCCSGRKTMEETVTAEEEEKAKLMTEMPTESEIEDFFVEAEKQLKEKFKKKYNFDFEKEKPLEGRYEWVKLE 0477A91E277C46B2 krp5_arath Cyclin-dependent kinase inhibitor 5 KRP5 ICK3 K7P8.10 At3g24810 KIP-related protein 5 Inhibitor/interactor of CDK protein 3 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGKYIKKSKVAGAVSVKDKSHPPALGFRTRAAAAKNLALHRLRSHSDEADSFNYLQLRSRRLVKLPLLTNTRKQQKQQLIPSVNQCQTKNPRASSGPAKKLEPDTTTEEACGDNERISRSDCNFGDKGFDLESENRSMISDSKSIQSEIEDFFASAEQQQQRFFIQKYNFDIVSDNPLPGRYEWVKVMP A66041310E8DDED6 krp2_arath Cyclin-dependent kinase inhibitor 2 KRP2 ICK2 T3A5.10 At3g50630 KIP-related protein 2 Inhibitor/interactor of CDK protein 2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAAVRRRERDVVEENGVTTTTVKRRKMEEEVDLVESRIILSPCVQATNRGGIVARNSAGASETSVVIVRRRDSPPVEEQCQIEEEDSSVSCCSTSEEKSKRRIEFVDLEENNGDDRETETSWIYDDLNKSEESMNMDSSSVAVEDVESRRRLRKSLHETVKEAELEDFFQVAEKDLRNKLLECSMKYNFDFEKDEPLGGGRYEWVKLNP 0E5EEFE9ED256B53 cdka1_arath Cyclin-dependent kinase A-1 CDKA-1 Cell division control protein 2 homolog A CDC2 At3g48750 CDC2A T21J18.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDQYEKVEKIGEGTYGVVYKARDKVTNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHSNIVKLQDVVHSEKRLYLVFEYLDLDLKKHMDSTPDFSKDLHMIKTYLYQILRGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGSHHYSTPVDIWSVGCIFAEMISQKPLFPGDSEIDQLFKIFRIMGTPYEDTWRGVTSLPDYKSAFPKWKPTDLETFVPNLDPDGVDLLSKMLLMDPTKRINARAALEHEYFKDLGGMP B5FAE55FA9EC366E cdka_1-krp1-1 2 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Among the 68 ICK (Interactors of Cdc2 Kinase) clones identified using Cdc2a as the bait ( Wang et al. 1997), 55 represented various lengths of ICK1, seven of ICK2 and six of ICK3. cdka_1-krp2-1 2 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Among the 68 ICK (Interactors of Cdc2 Kinase) clones identified using Cdc2a as the bait ( Wang et al. 1997), 55 represented various lengths of ICK1, seven of ICK2 and six of ICK3. cdka_1-krp5-1 2 7 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Among the 68 ICK (Interactors of Cdc2 Kinase) clones identified using Cdc2a as the bait ( Wang et al. 1997), 55 represented various lengths of ICK1, seven of ICK2 and six of ICK3. cdka_1-krp1-2 3 4 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Direct interaction of ICK1 with both Cdc2a Figure 5 cdka_1-krp1-3 1 7 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association The most significant reduction in β-galactosidase activity for the interaction of ICK1 with CycD3 resulted from the deletion of amino acids 109�153, whereas the deletion of amino acids 176�191 had a more detrimental effect on the interaction with Cdc2a. Figure 6