UniProt UniProt http://www.uniprot.org BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. Oncogene (0950-9232) 1998 imex curation Jensen DE., Proctor M., Marquis ST., Gardner HP., Ha SI., Chodosh LA., Ishov AM., Tommerup N., Vissing H., Sekido Y., Minna J., Borodovsky A., Schultz DC., Wilkinson KD., Maul GG., Barlev N., Berger SL., Prendergast GC., Rauscher FJ. no e-mail given Human adult B cell cDNA orchard Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Jensen DE., Proctor M., Marquis ST., Gardner HP., Ha SI., Chodosh LA., Ishov AM., Tommerup N., Vissing H., Sekido Y., Minna J., Borodovsky A., Schultz DC., Wilkinson KD., Maul GG., Barlev N., Berger SL., Prendergast GC., Rauscher FJ. no e-mail given Accepted 2008-NOV-27 by ORCHARD. Human adult B cell cDNA Oncogene (0950-9232) 1998 orchard Only protein-protein interactions imex curation BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. Oncogene (0950-9232) 1998 imex curation Jensen DE., Proctor M., Marquis ST., Gardner HP., Ha SI., Chodosh LA., Ishov AM., Tommerup N., Vissing H., Sekido Y., Minna J., Borodovsky A., Schultz DC., Wilkinson KD., Maul GG., Barlev N., Berger SL., Prendergast GC., Rauscher FJ. no e-mail given Human adult B cell cDNA orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Jensen DE., Proctor M., Marquis ST., Gardner HP., Ha SI., Chodosh LA., Ishov AM., Tommerup N., Vissing H., Sekido Y., Minna J., Borodovsky A., Schultz DC., Wilkinson KD., Maul GG., Barlev N., Berger SL., Prendergast GC., Rauscher FJ. no e-mail given Accepted 2008-NOV-27 by ORCHARD. Human adult B cell cDNA Oncogene (0950-9232) 1998 orchard Only protein-protein interactions imex curation BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. Oncogene (0950-9232) 1998 imex curation Jensen DE., Proctor M., Marquis ST., Gardner HP., Ha SI., Chodosh LA., Ishov AM., Tommerup N., Vissing H., Sekido Y., Minna J., Borodovsky A., Schultz DC., Wilkinson KD., Maul GG., Barlev N., Berger SL., Prendergast GC., Rauscher FJ. no e-mail given Human adult B cell cDNA orchard Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip weight by staining molecular weight estimation by staining weight by staining Jensen DE., Proctor M., Marquis ST., Gardner HP., Ha SI., Chodosh LA., Ishov AM., Tommerup N., Vissing H., Sekido Y., Minna J., Borodovsky A., Schultz DC., Wilkinson KD., Maul GG., Barlev N., Berger SL., Prendergast GC., Rauscher FJ. no e-mail given Transfected cells were treated with ALLN (N-acetyl-leucyl-leucyl-norleucinal) 20h prior to harvest in a parallel set. Immunoprecipitated proteins were visualized by SDS-PAGE and fluorography. Accepted 2008-NOV-27 by ORCHARD. Human adult B cell cDNA Oncogene (0950-9232) 1998 orchard Only protein-protein interactions imex curation BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. Oncogene (0950-9232) 1998 imex curation Jensen DE., Proctor M., Marquis ST., Gardner HP., Ha SI., Chodosh LA., Ishov AM., Tommerup N., Vissing H., Sekido Y., Minna J., Borodovsky A., Schultz DC., Wilkinson KD., Maul GG., Barlev N., Berger SL., Prendergast GC., Rauscher FJ. no e-mail given Human adult B cell cDNA orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Jensen DE., Proctor M., Marquis ST., Gardner HP., Ha SI., Chodosh LA., Ishov AM., Tommerup N., Vissing H., Sekido Y., Minna J., Borodovsky A., Schultz DC., Wilkinson KD., Maul GG., Barlev N., Berger SL., Prendergast GC., Rauscher FJ. no e-mail given Accepted 2008-NOV-27 by ORCHARD. Human adult B cell cDNA Oncogene (0950-9232) 1998 orchard Only protein-protein interactions imex curation BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. Oncogene (0950-9232) 1998 imex curation Jensen DE., Proctor M., Marquis ST., Gardner HP., Ha SI., Chodosh LA., Ishov AM., Tommerup N., Vissing H., Sekido Y., Minna J., Borodovsky A., Schultz DC., Wilkinson KD., Maul GG., Barlev N., Berger SL., Prendergast GC., Rauscher FJ. no e-mail given Human adult B cell cDNA orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Jensen DE., Proctor M., Marquis ST., Gardner HP., Ha SI., Chodosh LA., Ishov AM., Tommerup N., Vissing H., Sekido Y., Minna J., Borodovsky A., Schultz DC., Wilkinson KD., Maul GG., Barlev N., Berger SL., Prendergast GC., Rauscher FJ. no e-mail given Accepted 2008-NOV-27 by ORCHARD. Human adult B cell cDNA Oncogene (0950-9232) 1998 orchard Only protein-protein interactions imex curation BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. BAP1: a novel ubiquitin hydrolase which binds to the BRCA1 RING finger and enhances BRCA1-mediated cell growth suppression. Oncogene (0950-9232) 1998 imex curation Jensen DE., Proctor M., Marquis ST., Gardner HP., Ha SI., Chodosh LA., Ishov AM., Tommerup N., Vissing H., Sekido Y., Minna J., Borodovsky A., Schultz DC., Wilkinson KD., Maul GG., Barlev N., Berger SL., Prendergast GC., Rauscher FJ. no e-mail given Human adult B cell cDNA orchard Only protein-protein interactions human-rh30 Homo sapiens RH-30 rhabdomyosarcoma cell line rh30 RH-30 rhabdomyosarcoma cell line confocal microscopy confocal microscopy immunostaining immunostaining Jensen DE., Proctor M., Marquis ST., Gardner HP., Ha SI., Chodosh LA., Ishov AM., Tommerup N., Vissing H., Sekido Y., Minna J., Borodovsky A., Schultz DC., Wilkinson KD., Maul GG., Barlev N., Berger SL., Prendergast GC., Rauscher FJ. no e-mail given Accepted 2008-NOV-27 by ORCHARD. Human adult B cell cDNA Oncogene (0950-9232) 1998 orchard Only protein-protein interactions imex curation bap1_mouse Ubiquitin carboxyl-terminal hydrolase BAP1 Bap1 Kiaa0272 BRCA1-associated protein 1 Ubiquitin C-terminal hydrolase X4 protein protein mouse Mus musculus Mouse MNKGWLELESDPGLFTLLVEDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVSTLVDDTSVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSNVDLGPTLSRMKDFTKGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVRTMEAFHFVSYVPITGRLFELDGLKVYPIDHGPWGEDEEWTDKARRVIMERIGLATAGEPYHDIRFNLMAVVPDRRIKYETRLHVLKVNRQTVLEALQQLIRVTQPELIQTHKSQESQLPEESKPASSKSPLGLEAGRTPVASECTQTDGAEEVAGSCPQTTTHSPPSKCKLVVKPPGSSLNGVPPNPAPIVQRLPAFLDNHNYAKSPMQEEEDLAAGVGRSRVPVRAPQQYSEDEDDYEDEDEDVQNTNPAIRYKRKGTGKPGSLSNSSDGQLSVLQPNTINVLTEKLQESQKDLSVPLSIKTSSGAGSPAVAVPTHSQPSPTPSNESTDTASEIGSAFNSPLRSPIRSANPTRPSSPVTSHISKVLFGEDDSLLRVDCIRYNRAVRDLGPVISTGLLHLAEDGVLSPLALTEGGKGSSPSTRSSQGSQGSSGLEEKEVVEVTESRDKPGLNRSSEPLSGEKYSPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICTFISMLAQEGMLANLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAKRQ 2BDEAFB09CD276DB p38398-5 Breast cancer type 1 susceptibility protein BRCA1 RNF53 RING finger protein 53 Delta11 Delta772-3095 RING-type E3 ubiquitin transferase BRCA1 protein protein human Homo sapiens Human MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY A4EED8A734FCB619 bap1_human Ubiquitin carboxyl-terminal hydrolase BAP1 BAP1 KIAA0272 hucep-6 BRCA1-associated protein 1 Cerebral protein 6 protein protein human Homo sapiens Human MNKGWLELESDPGLFTLLVEDFGVKGVQVEEIYDLQSKCQGPVYGFIFLFKWIEERRSRRKVSTLVDDTSVIDDDIVNNMFFAHQLIPNSCATHALLSVLLNCSSVDLGPTLSRMKDFTKGFSPESKGYAIGNAPELAKAHNSHARPEPRHLPEKQNGLSAVRTMEAFHFVSYVPITGRLFELDGLKVYPIDHGPWGEDEEWTDKARRVIMERIGLATAGEPYHDIRFNLMAVVPDRRIKYEARLHVLKVNRQTVLEALQQLIRVTQPELIQTHKSQESQLPEESKSASNKSPLVLEANRAPAASEGNHTDGAEEAAGSCAQAPSHSPPNKPKLVVKPPGSSLNGVHPNPTPIVQRLPAFLDNHNYAKSPMQEEEDLAAGVGRSRVPVRPPQQYSDDEDDYEDDEEDDVQNTNSALRYKGKGTGKPGALSGSADGQLSVLQPNTINVLAEKLKESQKDLSIPLSIKTSSGAGSPAVAVPTHSQPSPTPSNESTDTASEIGSAFNSPLRSPIRSANPTRPSSPVTSHISKVLFGEDDSLLRVDCIRYNRAVRDLGPVISTGLLHLAEDGVLSPLALTEGGKGSSPSIRPIQGSQGSSSPVEKEVVEATDSREKTGMVRPGEPLSGEKYSPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICTFISMLAQEGMLANLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAKRQ DIP protein Q92560 original sequence version: 79 031DA03AE1841D85 brca1_human Breast cancer type 1 susceptibility protein BRCA1 RNF53 RING finger protein 53 RING-type E3 ubiquitin transferase BRCA1 protein protein human Homo sapiens Human MDLSALRVEEVQNVINAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIICAFQLDTGLEYANSYNFAKKENNSPEHLKDEVSIIQSMGYRNRAKRLLQSEPENPSLQETSLSVQLSNLGTVRTLRTKQRIQPQKTSVYIELGSDSSEDTVNKATYCSVGDQELLQITPQGTRDEISLDSAKKAACEFSETDVTNTEHHQPSNNDLNTTEKRAAERHPEKYQGSSVSNLHVEPCGTNTHASSLQHENSSLLLTKDRMNVEKAEFCNKSKQPGLARSQHNRWAGSKETCNDRRTPSTEKKVDLNADPLCERKEWNKQKLPCSENPRDTEDVPWITLNSSIQKVNEWFSRSDELLGSDDSHDGESESNAKVADVLDVLNEVDEYSGSSEKIDLLASDPHEALICKSERVHSKSVESNIEDKIFGKTYRKKASLPNLSHVTENLIIGAFVTEPQIIQERPLTNKLKRKRRPTSGLHPEDFIKKADLAVQKTPEMINQGTNQTEQNGQVMNITNSGHENKTKGDSIQNEKNPNPIESLEKESAFKTKAEPISSSISNMELELNIHNSKAPKKNRLRRKSSTRHIHALELVVSRNLSPPNCTELQIDSCSSSEEIKKKKYNQMPVRHSRNLQLMEGKEPATGAKKSNKPNEQTSKRHDSDTFPELKLTNAPGSFTKCSNTSELKEFVNPSLPREEKEEKLETVKVSNNAEDPKDLMLSGERVLQTERSVESSSISLVPGTDYGTQESISLLEVSTLGKAKTEPNKCVSQCAAFENPKGLIHGCSKDNRNDTEGFKYPLGHEVNHSRETSIEMEESELDAQYLQNTFKVSKRQSFAPFSNPGNAEEECATFSAHSGSLKKQSPKVTFECEQKEENQGKNESNIKPVQTVNITAGFPVVGQKDKPVDNAKCSIKGGSRFCLSSQFRGNETGLITPNKHGLLQNPYRIPPLFPIKSFVKTKCKKNLLEENFEEHSMSPEREMGNENIPSTVSTISRNNIRENVFKEASSSNINEVGSSTNEVGSSINEIGSSDENIQAELGRNRGPKLNAMLRLGVLQPEVYKQSLPGSNCKHPEIKKQEYEEVVQTVNTDFSPYLISDNLEQPMGSSHASQVCSETPDDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSPSPFTHTHLAQGYRRGAKKLESSEENLSSEDEELPCFQHLLFGKVNNIPSQSTRHSTVATECLSKNTEENLLSLKNSLNDCSNQVILAKASQEHHLSEETKCSASLFSSQCSELEDLTANTNTQDPFLIGSSKQMRHQSESQGVGLSDKELVSDDEERGTGLEENNQEEQSMDSNLGEAASGCESETSVSEDCSGLSSQSDILTTQQRDTMQHNLIKLQQEMAELEAVLEQHGSQPSNSYPSIISDSSALEDLRNPEQSTSEKAVLTSQKSSEYPISQNPEGLSADKFEVSADSSTSKNKEPGVERSSPSKCPSLDDRWYMHSCSGSLQNRNYPSQEELIKVVDVEEQQLEESGPHDLTETSYLPRQDLEGTPYLESGISLFSDDPESDPSEDRAPESARVGNIPSSTSALKVPQLKVAESAQSPAAAHTTDTAGYNAMEESVSREKPELTASTERVNKRMSMVVSGLTPEEFMLVYKFARKHHITLTNLITEETTHVVMKTDAEFVCERTLKYFLGIAGGKWVVSYFWVTQSIKERKMLNEHDFEVRGDVVNGRNHQGPKRARESQDRKIFRGLEICCYGPFTNMPTDQLEWMVQLCGASVVKELSSFTLGTGVHPIVVVQPDAWTEDNGFHAIGQMCEAPVVTREWVLDSVALYQCQELDTYLIPQIPHSHY DIP protein P38398 original sequence version: 183 DIP protein P38398 original sequence version: 111 DIP protein P38398 original sequence version: 149 DIP protein P38398-1 original sequence version: 149 DIP protein P38398 original sequence version: 142 DIP protein P38398 original sequence version: 212 DIP protein P38398 original sequence version: 163 DIP protein P38398 original sequence version: 185 DIP protein P38398 original sequence version: 148 DIP protein P38398 original sequence version: 210 89C6D83FF56312AF brca1-bard1-8 5 9 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait P38398:p.Cys61Gly mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain C G Wed May 02 17:10:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys61gly' Tue Jun 30 16:43:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys61Gly' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P38398:p.Met18Thr mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain M T Wed May 02 19:47:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met18thr' Tue Jun 30 18:28:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met18Thr' P38398:p.Cys64Gly mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain C G Wed May 02 15:17:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys64gly' Tue Jun 30 18:28:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys64Gly' physical association physical association Following BRCA1 bait constructs were not interacting: BRCA1-delAG185 (frameshift at aa pos. 22, insertion of 17 aa and premature stop); BRCA1-del31 (truncated after aa pos 31, PCR error). Author also stated that BRCA1 (P38398) mutant cys61gly disrupated the interaction but this data was not shown. 1B and 1C brca1-bard1-9 4 7 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P38398:p.Cys64Gly mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain C G Wed May 02 16:19:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys64gly' Tue Jun 30 17:39:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys64Gly' physical association physical association A BRCA1 construct BRCA1-del31 with a frameshift at aa pos 31 (PCR error) resulting in a 35 aa truncated BRCA1 product did not interact. 1c brca1-bard1-11 2 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q99PU7:p.Leu690Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L P Wed May 02 20:16:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu690pro' Tue Jun 30 18:42:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu690Pro' 10 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Following BRCA1 bait constructs were not interacting: BRCA1-del31 (truncated after aa pos 31, PCR error). A mBAP1 518del718 prey construct interacted very poorly. Various deletion constructs of the mBAP1 596-721 (amino- and carboxyterminal)showed no interaction. 1d mBAP1 mutation position was adjusted to SP (human numbering used in paper) bap1-brca1 1 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 4a Association of BRCA1 with BAP1 was tested in vitro by binding of full-length BRCA1 to hBAP1(483 +/- 729) fused to glutathione S-transferase (GST; Figure 4a). brca1-bap1 3 8 unspecified role unspecified role bait bait chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 9 unspecified role unspecified role prey prey chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 physical association physical association 4b Several attempts to transiently express BRCA1 to any significant level in COS1 cells were without success. Therefore, we performed the analysis with BRCA1-delta11, a naturally occurring splice variant ALLN (N-acetyl-leucyl-leucyl-norleucinal) Co-transfection of COS1 cells with BRCA1-D11 and BAP1 revealed that these proteins could be co-immunoprecipitated using either anti-BAP1 or anti-BRCA1 antibodies brca1-bap1-1 3 9 unspecified role unspecified role bait bait chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 8 unspecified role unspecified role prey prey chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 physical association physical association 4b Several attempts to transiently express BRCA1 to any significant level in COS1 cells were without success. Therefore, we performed the analysis with BRCA1-delta11, a naturally occurring splice variant ALLN (N-acetyl-leucyl-leucyl-norleucinal) Co-transfection of COS1 cells with BRCA1-D11 and BAP1 revealed that these proteins could be co-immunoprecipitated using either anti-BAP1 or anti-BRCA1 antibodies brca1-bap1-2 6 9 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization 4c