IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Interaction of the maize and Arabidopsis kinase interaction domains with a subset of receptor-like protein kinases: implications for transmembrane signaling in plants. Interaction of the maize and Arabidopsis kinase interaction domains with a subset of receptor-like protein kinases: implications for transmembrane signaling in plants. Plant J. (0960-7412) 1997 imex curation Braun DM., Stone JM., Walker JC. The identity of the Maize KAPP interacting protein called KBK1 in the text could not be identified unambiguously and was therefore not curated. A cDNA library of Maize leaf was screened with a radiolabelled probe made from the KI region of the Maize KAPP gene. braun@biosci.mbp.missouri.edu 14-Nov-2008: Contacted by JYOTI. Maize leaf cDNA library. jyoti Only protein-protein interactions in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Braun DM., Stone JM., Walker JC. The identity of the Maize KAPP interacting protein called KBK1 in the text could not be identified unambiguously and was therefore not curated. A cDNA library of Maize leaf was screened with a radiolabelled probe made from the KI region of the Maize KAPP gene. braun@biosci.mbp.missouri.edu Accepted 2008-DEC-5 by JYOTI. 14-Nov-2008: Contacted by JYOTI. Maize leaf cDNA library. Plant J. (0960-7412) 1997 jyoti Only protein-protein interactions imex curation Interaction of the maize and Arabidopsis kinase interaction domains with a subset of receptor-like protein kinases: implications for transmembrane signaling in plants. Interaction of the maize and Arabidopsis kinase interaction domains with a subset of receptor-like protein kinases: implications for transmembrane signaling in plants. Plant J. (0960-7412) 1997 imex curation Braun DM., Stone JM., Walker JC. The identity of the Maize KAPP interacting protein called KBK1 in the text could not be identified unambiguously and was therefore not curated. A cDNA library of Maize leaf was screened with a radiolabelled probe made from the KI region of the Maize KAPP gene. braun@biosci.mbp.missouri.edu 14-Nov-2008: Contacted by JYOTI. Maize leaf cDNA library. jyoti Only protein-protein interactions in vitro In vitro biochemical biochemical nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Braun DM., Stone JM., Walker JC. The identity of the Maize KAPP interacting protein called KBK1 in the text could not be identified unambiguously and was therefore not curated. A cDNA library of Maize leaf was screened with a radiolabelled probe made from the KI region of the Maize KAPP gene. braun@biosci.mbp.missouri.edu Accepted 2008-DEC-5 by JYOTI. Maize leaf cDNA library. Plant J. (0960-7412) 1997 jyoti Only protein-protein interactions imex curation Interaction of the maize and Arabidopsis kinase interaction domains with a subset of receptor-like protein kinases: implications for transmembrane signaling in plants. Interaction of the maize and Arabidopsis kinase interaction domains with a subset of receptor-like protein kinases: implications for transmembrane signaling in plants. Plant J. (0960-7412) 1997 imex curation Braun DM., Stone JM., Walker JC. The identity of the Maize KAPP interacting protein called KBK1 in the text could not be identified unambiguously and was therefore not curated. A cDNA library of Maize leaf was screened with a radiolabelled probe made from the KI region of the Maize KAPP gene. braun@biosci.mbp.missouri.edu 14-Nov-2008: Contacted by JYOTI. Maize leaf cDNA library. jyoti Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Braun DM., Stone JM., Walker JC. The identity of the Maize KAPP interacting protein called KBK1 in the text could not be identified unambiguously and was therefore not curated. A cDNA library of Maize leaf was screened with a radiolabelled probe made from the KI region of the Maize KAPP gene. braun@biosci.mbp.missouri.edu Accepted 2008-DEC-5 by JYOTI. 14-Nov-2008: Contacted by JYOTI. Maize leaf cDNA library. Plant J. (0960-7412) 1997 jyoti Only protein-protein interactions imex curation o49973_maize Protein-serine/threonine phosphatase KAPP 542351 ZEAMMB73_Zm00001d019169 protein protein maize Zea mays (Maize) Maize MAVPPLLLVAVAVAALSILVLVVFAFRRCRRRRQRHPLQAAAVAPTPVAVQDEDIDRPLLSENQSDHSRQNISFLGSSVGEPSKIQTNRSNTSPRSHAIADTGRIYPAECCVTQGETHVINVENDTSEEFQLGSTLRRTPPPKWPTPDQKHRRRVSGDDNHNGSVPLKDNTYHSRLDLEVIAGPSHGISCSRQSSRPSMLPITLGRVPPSDLVLKDSEVSGKHAQINWNGKTLKWELVDMGSLNGTFLNSQAVHHPSAGSRHWGEPAELAHGDIITLGTSSKLSVQILLQNQRVPAGVGMASDPMVARRSGKKLPMEDISFCQCPLQGVEHFGLFGIFDGHGGDGAAKAVSKILPEHLGYILSHPETKERVQSYSDASDVLRYAFTLTEDTIDHQYEGCTGTALLIWFDQNKDCFAQCANLGDSACVMSVNGKTIDMTEDHRVTSATERARIARTGQPLRDGEARLSGLNLARMFGDKFLKEQDPRFSSEPYVSQAVRITKACTAFAVIASDGLWDVISTKRAVQLVVEGKERRSSGDATSAAARVASRVLDEARSLRTKDNTSVIFVDFDVLRRDPCISPVD A9FBEBA95634A690 kpro_maize Putative receptor protein kinase ZmPK1 PK1 protein protein maize Zea mays (Maize) Maize MPRPLAALLSTACILSFFIALFPRAASSRDILPLGSSLVVESYESSTLQSSDGTFSSGFYEVYTHAFTFSVWYSKTEAAAANNKTIVWSANPDRPVHARRSALTLQKDGNMVLTDYDGAAVWRADGNNFTGVQRARLLDTGNLVIEDSGGNTVWQSFDSPTDTFLPTQLITAATRLVPTTQSRSPGNYIFRFSDLSVLSLIYHVPQVSDIYWPDPDQNLYQDGRNQYNSTRLGMLTDSGVLASSDFADGQALVASDVGPGVKRRLTLDPDGNLRLYSMNDSDGSWSVSMVAMTQPCNIHGLCGPNGICHYSPTPTCSCPPGYATRNPGNWTEGCMAIVNTTCDRYDKRSMRFVRLPNTDFWGSDQQHLLSVSLRTCRDICISDCTCKGFQYQEGTGSCYPKAYLFSGRTYPTSDVRTIYLKLPTGVSVSNALIPRSDVFDSVPRRLDCDRMNKSIREPFPDVHKTGGGESKWFYFYGFIAAFFVVEVSFISFAWFFVLKRELRPSELWASEKGYKAMTSNFRRYSYRELVKATRKFKVELGRGESGTVYKGVLEDDRHVAVKKLENVRQGKEVFQAELSVIGRINHMNLVRIWGFCSEGSHRLLVSEYVENGSLANILFSEGGNILLDWEGRFNIALGVAKGLAYLHHECLEWVIHCDVKPENILLDQAFEPKITDFGLVKLLNRGGSTQNVSHVRGTLGYIAPEWVSSLPITAKVDVYSYGVVLLELLTGTRVSELVGGTDEVHSMLRKLVRMLSAKLEGEEQSWIDGYLDSKLNRPVNYVQARTLIKLAVSCLEEDRSKRPTMEHAVQTLLSADD F164B44719922E67 cri4_maize Putative receptor protein kinase CRINKLY4 CR4 protein protein maize Zea mays (Maize) Maize MDHVPALVLAGCCFLALLPGWACGLGSMSSIAVSYGEDGPVFCGLNSDGSHLVACFGADASVLYGAPPNIPFLGLTAGDGFVCGLLLDTRQPYCWGSNSYVKSGVPQPMVEGARYSELSAGDNHLCALRAAQDGGRGSSAATSLIDCWGYNMTATHAVDEAVSTVSAGSVFNCGLFARNRTVFCWGDETVSGVVGLAPRDLHFQSIGAGGYHVCGVLENAQVFCWGRSLEMQQVVPSSAIGDGDVNIVPMDAMSTVVGGRFHACGIRSLDHQVACWGFTLHNSTSPPKGLKMYALVAGDYFTCGVPAETSLMPRCWGNSGPLALPMAVPPGICVPTACSHGYYEYVNHGEVGSIKVCKPANSRLCLPCSTGCPEGLYESSPCNATADRVCQFDCLKCVTDECLSFCLSQKRTKSRKLMAFQMRIFVAEIVFAVVLVLSVSVTTCLYVRHKLRHCQCSNRELRLAKSTAYSFRKDNMKIQPDMEDLKIRRAQEFSYEELEQATGGFSEDSQVGKGSFSCVFKGILRDGTVVAVKRAIKASDVKKSSKEFHNELDLLSRLNHAHLLNLLGYCEDGSERLLVYEFMAHGSLYQHLHGKDPNLKKRLNWARRVTIAVQAARGIEYLHGYACPPVIHRDIKSSNILIDEDHNARVADFGLSILGPADSGTPLSELPAGTLGYLDPEYYRLHYLTTKSDVYSFGVVLLEILSGRKAIDMQFEEGNIVEWAVPLIKAGDIFAILDPVLSPPSDLEALKKIASVACKCVRMRGKDRPSMDKVTTALEHALALLMGSPCIEQPILPTEVVLGSSRMHKVSQMSSNHSCSENELADGEDQGIGYRAPSWITFPSVTSSQRRKSSASEADIVGRRATDGRNVGSSIGDGLRSLEEEIAPASPQENLYLQHNF 52F8481AC187E061 q43393_brana Receptor-like serine/threonine-protein kinase protein protein brana Brassica napus (Rape) MKGVRNIYHHSYTSFLLLFLVMILIHPALSIYINTLSSTESLTISSNRTLVSPGDVFELGFFETNSRWYLGMWYKKLPFRTYVWVANRDNPLSNSIGTLKISGNNLVILGHSNKSVWSTNLTRGIDRSTVVAELLANGNFVMRDSNNNDASQFLWQSFDYPTDTLLPEMKLGNDLKTGLNRFLTSWRSSDDPSSGEFLYELETGRLPEFYLSKGIFPAYRSGPWNGIRFSGIPDDQKLSYLVYNFTENSEEVVYTFRMTNNSIYSKLTVSLSGYFERQTWNASLGMWNVSWSLPLPSQCDTYRRCGPYAYCDVSTSPICNCIQGFNPSNVQQWDQRSWSGGCIRRTRLSCSGDGFTRMENMELPETTMAIVDRSIGVKECKKRCLSDCNCTAFANADVQNGGTGCIIWAGELEDIRNYAADGQDLYVRLAAADLVKRRNANGQIISLTVGVSVLLLLIMFCLWKRKQKRANANATSIANRQRNQNLPMNGMVLSSKREFLEEKKIEELELPLIELETVVKATENFSNCNKLGQGGFGIVYKGRLLDGQEIAVKRLSKTSVQGTDEFMNEVTLIARLQHINLVQIIGCCIEADEKMLVYEYLENLSLDSYLFGKTRRSKLNWKERFDIINGVARGLLYLHQDSRFRIIHRDLKVSNILLDKNMTPKISDFGMARIFARDETEANTVKVVGTYGYMSPEYAMHEIFSEKSDVFSFGVIVLEIVSGKKNSYNLNYKNNLLSYAWSQWEEGRALEIIDPVIVDSLPSTFQPQEVLKCIQIGLLCVQELAEHRPTMSSVVWMLGSEAKEIPQPKQPGHCIGRSPYDLDPSSSSQCDDDESWTVNQYTCSLVDAR 9EF3AAD47831ECCE o49974_maize Receptor-like serine/threonine-protein kinase KIK1 protein protein maize Zea mays (Maize) Maize MVSSPRLLFLLLAGASLCCVAAQKTDTLRQGESLSGAATLVSSPEGVFEAGFFAPDPKQPSRQYLGIWYHSISPRTVVWVANRVAPATSASPSLTLTVTGDLRVLDGTAANGTADAPLLWSSNTTSRAGPRGGYSAVLQDTGSLEVRSEDGVLWDSFWHPTDTILSGMRITLQAPGRGPKERMLFTSWASETDPSPGRYALGLDPGNSGQAYIWKDGNVTYWRSGQWNGVNFIGIPWRPLYRSGFTPAIDPVLGNYYTYTATNTSLQRFVVLPNGTDICYMVRKSSQDWELVWYQPSNECEYYATCGPNAKCTASQDGKAKCTCLKGFHPKLQEQWNAGNWSQGCIRSPPLGCETNQSGDGFLPMGNIKWPDFSYWVSTVGDEPGCRTVCLNNCSCGAYVYTATTGCLAWGNELIDMHELQTGAYTLNLKLPASELRGHHPIWKIATIISAIVLFVLAACLLLWWKHGRNIKDAVHGSWRSRHSSTQSQQNSAMLDISQSIRFDDDVEDGKSHELKVYSLDRIRTATSNFSDSNKLGEGGFGPVYMGTLPGGEEVAVKRLCRNSGQGLEEFKNEVILIAKLQHRNLVRLLGCCIPREEKILVYEYMPNKSLDAFLFNPEKQRLLDWKKRFDIIEGIARGLLYLHRDSRLRVVHRDLKASNILLDADMKPKISDFGMARMFGGDQNQFNTNRVVGTFGYMSPEYAMEGIFSVKSDVYGFGVLILEIITGKRAVSFHCHEDSLNIAGYAWRQWNEDNAAELIDPVIRASCSVRQVLRCIHIALLCVQDHADERPDIPTVILMLSNDSSSLPNPRPPTLMLRGREIESSKSSEKDRSHSIGTVTMTQLHGR FF479F90477A25EA p2c70_arath Protein phosphatase 2C 70 KAPP Kinase-associated protein phosphatase Protein ROOT ATTENUATED GROWTH 1 RAG1 F7K24.30 T24G5.3 At5g19280 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAMIGMNIIGLFMVLMLLLISLIILFACKPWRYFSRFRSSSRFSSTFKVGDLQRPLISDDGNLIQGQTSEVTREYDLEGACYQNDGLLHSSLTEGRFYKQRLPSSSPHFSQGESFVLEVISEPSDNALVGQTLKLPAEKGSLAEVQTYDWQNNRNENLQYNLEKDRLINLSPRLVEDQRSWLFLEVIAGPAIGLQHAVNSTSSSKLPVKLGRVSPSDLALKDSEVSGKHAQITWNSTKFKWELVDMGSLNGTLVNSHSISHPDLGSRKWGNPVELASDDIITLGTTTKVYVRISSQNEFQIPFKIGVASDPMAMRRGGRKLPMEDVCHYKWPLPGANKFGLFCVCDGHGGSGAAQSAIKIIPEVLANILSDSLRKEKVLSKRDASDVLRDMFAKTEARLEEHQYEGCTATVLLVWKDNEENFFAQCANLGDSACVIHLGGRYIQMTEDHRVVSLSERKRFQEAGLALRDGETRLFGINLARMLGDKFPKQQDSRFSAEPYISEPLRIDQSSKDVFAVLASDGLWDVVSPKKAVQLVLQMRDKERGRESSAEKIANGLLNEARAMRTKDNTSIIYLDFDTSL EE0C2A41E55E100D tmk1_arath Receptor protein kinase TMK1 TMK1 BARK1-like kinase 1 BLK1 Transmembrane kinase 1 F15E12.4 At1g66150 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKKRRTFLLFSFTFLLLLSLSKADSDGDLSAMLSLKKSLNPPSSFGWSDPDPCKWTHIVCTGTKRVTRIQIGHSGLQGTLSPDLRNLSELERLELQWNNISGPVPSLSGLASLQVLMLSNNNFDSIPSDVFQGLTSLQSVEIDNNPFKSWEIPESLRNASALQNFSANSANVSGSLPGFLGPDEFPGLSILHLAFNNLEGELPMSLAGSQVQSLWLNGQKLTGDITVLQNMTGLKEVWLHSNKFSGPLPDFSGLKELESLSLRDNSFTGPVPASLLSLESLKVVNLTNNHLQGPVPVFKSSVSVDLDKDSNSFCLSSPGECDPRVKSLLLIASSFDYPPRLAESWKGNDPCTNWIGIACSNGNITVISLEKMELTGTISPEFGAIKSLQRIILGINNLTGMIPQELTTLPNLKTLDVSSNKLFGKVPGFRSNVVVNTNGNPDIGKDKSSLSSPGSSSPSGGSGSGINGDKDRRGMKSSTFIGIIVGSVLGGLLSIFLIGLLVFCWYKKRQKRFSGSESSNAVVVHPRHSGSDNESVKITVAGSSVSVGGISDTYTLPGTSEVGDNIQMVEAGNMLISIQVLRSVTNNFSSDNILGSGGFGVVYKGELHDGTKIAVKRMENGVIAGKGFAEFKSEIAVLTKVRHRHLVTLLGYCLDGNEKLLVYEYMPQGTLSRHLFEWSEEGLKPLLWKQRLTLALDVARGVEYLHGLAHQSFIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKGSIETRIAGTFGYLAPEYAVTGRVTTKVDVYSFGVILMELITGRKSLDESQPEESIHLVSWFKRMYINKEASFKKAIDTTIDLDEETLASVHTVAELAGHCCAREPYQRPDMGHAVNILSSLVELWKPSDQNPEDIYGIDLDMSLPQALKKWQAYEGRSDLESSTSSLLPSLDNTQMSIPTRPYGFAESFTSVDGR DIP protein P43298 original sequence version: 131 93E300B52FF549DE crk6_arath Cysteine-rich receptor-like protein kinase 6 CRK6 RLK5 Receptor-like protein kinase 5 F7H19.330 F21P8.30 At4g23140 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSLISFNFLFLFSFLTSSFTASAQDPFYLNHYCPNTTTYSSNSTYSTNLRTLLSSLSSRNASYSTGFQNATAGKAPDRVTGLFLCRGDVSPEVCRNCVAFSVNQTLNLCPKVREAVFYYEQCILRYSHKNILSTAITNEGEFILSNTNTISPNQKQIDGFTSFVSSTMSEAAGKAANSSRKLYTVNTELTAYQNLYGLLQCTPDLTRADCLSCLQSSINGMALSRIGARLYWPSCTARYELYPFYNESAIETPPLPPPPPPPPPRESLVSTPPISSSSLPGKSGNSTVLVVAVVVLAVLLFIALVGYCFLAKKKKKTFDTASASEVGDDMATADSLQLDYRTIQTATNDFAESNKIGRGGFGEVYKGTFSNGKEVAVKRLSKNSRQGEAEFKTEVVVVAKLQHRNLVRLLGFSLQGEERILVYEYMPNKSLDCLLFDPTKQIQLDWMQRYNIIGGIARGILYLHQDSRLTIIHRDLKASNILLDADINPKIADFGMARIFGLDQTQDNTSRIVGTYGYMAPEYAMHGQFSMKSDVYSFGVLVLEIISGRKNSSFGESDGAQDLLTHAWRLWTNKKALDLVDPLIAENCQNSEVVRCIHIGLLCVQEDPAKRPAISTVFMMLTSNTVTLPVPRQPGFFIQCRAVKDPLDSDQSTTTKSFPASIDDESITDLYPR 06049FF8A3CE8FC4 crk10_arath Cysteine-rich receptor-like protein kinase 10 CRK10 RLK4 Receptor-like protein kinase 4 F21P8.70 At4g23180 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRRNTDQESPIMSYYSSFFFLFLFSFLTSFRVSAQDPTYVYHTCQNTANYTSNSTYNNNLKTLLASLSSRNASYSTGFQNATVGQAPDRVTGLFNCRGDVSTEVCRRCVSFAVNDTLTRCPNQKEATLYYDECVLRYSNQNILSTLITTGGVILVNTRNVTSNQLDLLSDLVLPTLNQAATVALNSSKKFGTRKNNFTALQSFYGLVQCTPDLTRQDCSRCLQLVINQIPTDRIGARIINPSCTSRYEIYAFYTESAVPPPPPPPSISTPPVSAPPRSGKDGNSKVLVIAIVVPIIVAVLLFIAGYCFLTRRARKSYYTPSAFAGDDITTADSLQLDYRTIQTATDDFVESNKIGQGGFGEVYKGTLSDGTEVAVKRLSKSSGQGEVEFKNEVVLVAKLQHRNLVRLLGFCLDGEERVLVYEYVPNKSLDYFLFDPAKKGQLDWTRRYKIIGGVARGILYLHQDSRLTIIHRDLKASNILLDADMNPKIADFGMARIFGLDQTEENTSRIVGTYGYMSPEYAMHGQYSMKSDVYSFGVLVLEIISGKKNSSFYQTDGAHDLVSYAWGLWSNGRPLELVDPAIVENCQRNEVVRCVHIGLLCVQEDPAERPTLSTIVLMLTSNTVTLPVPRQPGLFFQSRIGKDPLDTDTTSKSLLGSVDDASITDIHPR E2CCD6AAC5F87BF7 kapp-kapp 2 4 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 1 The authors state that "we screened a maize leaf cDNA library at reduced stringency using the Arabidopsis KI domain as a hybridization probe", however the amino acid coordinates of this region are not provided. The KI domain is thought to be equivilent to the FHA (IPR000253) domain recorded in the UniProt entry for this protein. This range has been used to represent the KI region and entered as 'fuzzy coordinates', (see IntAct annotation manual availaible on the IntAct website), to indicate the fact that the exact coordinates are unknown. To isolate maize kinase interaction domain-containing genes, we screened a maize leaf cDNA library at reduced stringency using the Arabidopsis KI domain as a hybridization probe. Approximately 3.2 x 105 plaques were screened and five positive clones identified. All positive clones corresponded to the maize homolog of KAPP; no other genes containing a KI domain were identified. The longestlength cDNA insert was sequenced and encodes the complete protein as shown by comparison to the Arabidopsis protein kapp-kik-3 2 8 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Figure 2 Characterization of KI domain interacting kinase 1 (KIK1). kik-4 3 8 putative self putative self putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O49974:p.Lys558Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K E Wed May 02 15:20:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys558glu' Tue Jun 30 17:44:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys558Glu' ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Figure 3 Figure 5b Recombinant proteins were expressed in E. coil, affinitypurified, incubated in the presence of [P-32p]ATP, and separated by SDS-PAGE. The KIK1CAT recombinant protein incorporates 32p while the site-directed mutated protein, K558E, is incapable of autophosphorylation O49974 was autophosphorylated (on a serine and/or threonine residue(s)). The authors concluded "these results establish that KIK1 is a serine/threonine protein kinase". kapp-kik-5 1 8 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 5C The maize and Arabidopsis KI domains bind to the catalytic domains of KIK1, Arabidopsis TMK1 (Chang et al., 1992), Brassica SRK-A14 (Glavin et al., 1994), Arabidopsis RLK5 (Horn and Walker, 1994), and Arabidopsis RLK4 (Walker, 1993), but not to the kinase domains of maize CRINKLY4 (Becraft et aL, 1996), or maize ZmPK1 tmk1 3 10 putative self putative self putative self putative self region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Figure 5b autophosphorylation All of the recombinant protein kinases are capable of autophosphorylating in vitro, while the protein affinity tag, maltose binding protein (MBP) does not incorporate any 32p (Figure 5b). The maize and Arabidopsis KI domains bind to the catalytic domains of KIK1, Arabidopsis TMK1 (Chang et al., 1992), Brassica SRK-A14 (Glavin et al., 1994), Arabidopsis RLK5 (Horn and Walker, 1994), and Arabidopsis RLK4 (Walker, 1993), but not to the kinase domains of maize CRINKLY4 (Becraft et aL, 1996), or maize ZmPK1 (Walker and Zhang, 1990), or to the protein affinity tag, crk6 3 11 putative self putative self putative self putative self region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Figure 5b autophosphorylation All of the recombinant protein kinases are capable of autophosphorylating in vitro, while the protein affinity tag, maltose binding protein (MBP) does not incorporate any 32p (Figure 5b). The maize and Arabidopsis KI domains bind to the catalytic domains of KIK1, Arabidopsis TMK1 (Chang et al., 1992), Brassica SRK-A14 (Glavin et al., 1994), Arabidopsis RLK5 (Horn and Walker, 1994), and Arabidopsis RLK4 (Walker, 1993), but not to the kinase domains of maize CRINKLY4 (Becraft et aL, 1996), or maize ZmPK1 (Walker and Zhang, 1990), or to the protein affinity tag, The authors state that a region of 649-999 amino acids were used in this assay. However the TAIR or UniProt record show that the CRK6 protein is 680 and 674 amino acids respectively. Note the TAIR record shows an insertion of the amino acids (FVVDSS) between amino acid 517 and 518 in the UniProt record. The protein RLK5 was renamed according to the Naming convention from Chen et al 2003 (PMID 14756307). crk10 3 12 putative self putative self putative self putative self region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Figure 5b autophosphorylation All of the recombinant protein kinases are capable of autophosphorylating in vitro, while the protein affinity tag, maltose binding protein (MBP) does not incorporate any 32p (Figure 5b). The maize and Arabidopsis KI domains bind to the catalytic domains of KIK1, Arabidopsis TMK1 (Chang et al., 1992), Brassica SRK-A14 (Glavin et al., 1994), Arabidopsis RLK5 (Horn and Walker, 1994), and Arabidopsis RLK4 (Walker, 1993), but not to the kinase domains of maize CRINKLY4 (Becraft et aL, 1996), or maize ZmPK1 (Walker and Zhang, 1990), or to the protein affinity tag, The authors state that a region of 427-797 amino acids were used in this assay. However the TAIR or UniProt record show that the CRK6 protein is 669 and 658 amino acids respectively. Note the TAIR record shows an insertion of the amino acids (MRRNTDQESPI) at the amino terminus of the UniProt record. The protein RLK4 was renamed according to the Naming convention from Chen et al 2003 (PMID 14756307). q43393 3 7 putative self putative self putative self putative self c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Figure 5b autophosphorylation All of the recombinant protein kinases are capable of autophosphorylating in vitro, while the protein affinity tag, maltose binding protein (MBP) does not incorporate any 32p (Figure 5b). The maize and Arabidopsis KI domains bind to the catalytic domains of KIK1, Arabidopsis TMK1 (Chang et al., 1992), Brassica SRK-A14 (Glavin et al., 1994), Arabidopsis RLK5 (Horn and Walker, 1994), and Arabidopsis RLK4 (Walker, 1993), but not to the kinase domains of maize CRINKLY4 (Becraft et aL, 1996), or maize ZmPK1 (Walker and Zhang, 1990), or to the protein affinity tag, pk1-2 3 5 putative self putative self putative self putative self region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Figure 5b autophosphorylation All of the recombinant protein kinases are capable of autophosphorylating in vitro, while the protein affinity tag, maltose binding protein (MBP) does not incorporate any 32p (Figure 5b). The maize and Arabidopsis KI domains bind to the catalytic domains of KIK1, Arabidopsis TMK1 (Chang et al., 1992), Brassica SRK-A14 (Glavin et al., 1994), Arabidopsis RLK5 (Horn and Walker, 1994), and Arabidopsis RLK4 (Walker, 1993), but not to the kinase domains of maize CRINKLY4 (Becraft et aL, 1996), or maize ZmPK1 (Walker and Zhang, 1990), or to the protein affinity tag, cr4 3 6 putative self putative self putative self putative self region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Figure 5b autophosphorylation All of the recombinant protein kinases are capable of autophosphorylating in vitro, while the protein affinity tag, maltose binding protein (MBP) does not incorporate any 32p (Figure 5b). The maize and Arabidopsis KI domains bind to the catalytic domains of KIK1, Arabidopsis TMK1 (Chang et al., 1992), Brassica SRK-A14 (Glavin et al., 1994), Arabidopsis RLK5 (Horn and Walker, 1994), and Arabidopsis RLK4 (Walker, 1993), but not to the kinase domains of maize CRINKLY4 (Becraft et aL, 1996), or maize ZmPK1 (Walker and Zhang, 1990), or to the protein affinity tag, kapp-tmk1 1 10 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 5C The maize and Arabidopsis KI domains bind to the catalytic domains of KIK1, Arabidopsis TMK1 (Chang et al., 1992), Brassica SRK-A14 (Glavin et al., 1994), Arabidopsis RLK5 (Horn and Walker, 1994), and Arabidopsis RLK4 (Walker, 1993), but not to the kinase domains of maize CRINKLY4 (Becraft et aL, 1996), or maize ZmPK1 kapp-q43393 1 7 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 5C kapp-crk6 1 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 5C The authors state that the region of the CRK6 protein used was 649 to 999 amino acids in length. However both UniProt and TAIR records show that the CRK10 protein is 647 amino acids and 670 amino acids respectively. Note the TAIR record differs a several regions in its sequence to the UniProt record. kapp-crk10 1 12 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 5C The authors state that the region of CRK10 used was 427 to 797 amino acids however both UniProt and TAIR records show the CRK10 protein to be 658 amino acids and 670 amino acids in length respectively. Note the TAIR entry has an N-terminal amino acid sequence ( MRRNTDQESPI) which the UniProt record lacks. kapp-tmk1-1 1 10 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 5B The authors do not provide the amino acid coordinates of the KI region of the Arabidopsis KAPP protein used in this study. The KI domain is thought to be equivilent to the FHA (IPR000253) domain recorded in the UniProt entry for this protein. This range has been used to represent the KI region and entered as 'fuzzy coordinates', (see IntAct annotation manual availaible on the IntAct website), to indicate the fact that the exact coordinates are unknown. kapp-q43393-1 1 7 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction The authors do not provide the amino acid coordinates of the KI region of the Arabidopsis KAPP protein used in this study. The KI domain is thought to be equivilent to the FHA (IPR000253) domain recorded in the UniProt entry for this protein. This range has been used to represent the KI region and entered as 'fuzzy coordinates', (see IntAct annotation manual availaible on the IntAct website), to indicate the fact that the exact coordinates are unknown. Figure 5B kapp-crk6-2 1 11 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction The authors do not provide the amino acid coordinates of the KI region of the Arabidopsis KAPP protein used in this study. The KI domain is thought to be equivilent to the FHA (IPR000253) domain recorded in the UniProt entry for this protein. This range has been used to represent the KI region and entered as 'fuzzy coordinates', (see IntAct annotation manual availaible on the IntAct website), to indicate the fact that the exact coordinates are unknown. Figure 5B The authors state that the region of the CRK6 protein used was 649 to 999 amino acids in length. However both UniProt and TAIR records show that the CRK10 protein is 647 amino acids and 670 amino acids respectively. Note the TAIR record differs a several regions in its sequence to the UniProt record. kapp-crk10-1 1 12 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 5B The authors state that the region of CRK10 used was 427 to 797 amino acids however both UniProt and TAIR records show the CRK10 protein to be 658 amino acids and 670 amino acids in length respectively. Note the TAIR entry has an N-terminal amino acid sequence ( MRRNTDQESPI) which the UniProt record lacks. The authors do not provide the amino acid coordinates of the KI region of the Arabidopsis KAPP protein used in this study. The KI domain is thought to be equivilent to the FHA (IPR000253) domain recorded in the UniProt entry for this protein. This range has been used to represent the KI region and entered as 'fuzzy coordinates', (see IntAct annotation manual availaible on the IntAct website), to indicate the fact that the exact coordinates are unknown. kapp-kik1-3 1 9 unspecified role unspecified role prey prey region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction The authors do not provide the amino acid coordinates of the KI region of the Arabidopsis KAPP protein used in this study. The KI domain is thought to be equivilent to the FHA (IPR000253) domain recorded in the UniProt entry for this protein. This range has been used to represent the KI region and entered as 'fuzzy coordinates', (see IntAct annotation manual availaible on the IntAct website), to indicate the fact that the exact coordinates are unknown. Figure 5B The authors state that the region of the CRK6 protein used was 649 to 999 amino acids in length. However both UniProt and TAIR records show that the CRK10 protein is 647 amino acids and 670 amino acids respectively. Note the TAIR record differs a several regions in its sequence to the UniProt record.