IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ AMPK and PPARdelta agonists are exercise mimetics. AMPK and PPARdelta agonists are exercise mimetics. Cell (0092-8674) 2008 imex curation Narkar VA., Downes M., Yu RT., Embler E., Wang YX., Banayo E., Mihaylova MM., Nelson MC., Zou Y., Juguilon H., Kang H., Shaw RJ., Evans RM. I have written to the authors and asked them to let me know the source of PGC1alpha - no reply to date so have not been able to annotate this data. The source of PGC1 alpha could not be traced so these interactions have not been added to the entry. Experiment s were performed in AD293 cells which are a sub-clone of HEK293 cells. Source of PPARdelta taken from PMID: 16467150. The source of PGC1 alpha could not be traced so these interactions have not been added to the entry. evans@salk.edu 16-Oct-2008: Contacted by JYOTI. davet Only protein-protein interactions 2 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Narkar VA., Downes M., Yu RT., Embler E., Wang YX., Banayo E., Mihaylova MM., Nelson MC., Zou Y., Juguilon H., Kang H., Shaw RJ., Evans RM. I have written to the authors and asked them to let me know the source of PGC1alpha - no reply to date so have not been able to annotate this data. The source of PGC1 alpha could not be traced so these interactions have not been added to the entry. Experiment s were performed in AD293 cells which are a sub-clone of HEK293 cells. Source of PPARdelta taken from PMID: 16467150. The source of PGC1 alpha could not be traced so these interactions have not been added to the entry. evans@salk.edu Accepted 2008-OCT-23 by DAVET. 16-Oct-2008: Contacted by JYOTI. Cell (0092-8674) 2008 davet Only protein-protein interactions 2 imex curation ppard_mouse Peroxisome proliferator-activated receptor delta Ppard Nr1c2 Pparb Peroxisome proliferator-activated receptor beta Nuclear receptor subfamily 1 group C member 2 Nuclear hormone receptor 1 protein protein mouse Mus musculus Mouse MEQPQEETPEAREEEKEEVAMGDGAPELNGGPEHTLPSSSCADLSQNSSPSSLLDQLQMGCDGASGGSLNMECRVCGDKASGFHYGVHACEGCKGFFRRTIRMKLEYEKCDRICKIQKKNRNKCQYCRFQKCLALGMSHNAIRFGRMPEAEKRKLVAGLTASEGCQHNPQLADLKAFSKHIYNAYLKNFNMTKKKARSILTGKSSHNAPFVIHDIETLWQAEKGLVWKQLVNGLPPYNEISVHVFYRCQSTTVETVRELTEFAKNIPNFSSLFLNDQVTLLKYGVHEAIFAMLASIVNKDGLLVANGSGFVTHEFLRSLRKPFSDIIEPKFEFAVKFNALELDDSDLALFIAAIILCGDRPGLMNVPQVEAIQDTILRALEFHLQVNHPDSQYLFPKLLQKMADLRQLVTEHAQMMQWLKKTESETLLHPLLQEIYKDMY DIP protein P35396 original sequence version: 85 58E0F595DD193FDA aapk1_human 5'-AMP-activated protein kinase catalytic subunit alpha-1 PRKAA1 AMPK1 Tau-protein kinase PRKAA1 Acetyl-CoA carboxylase kinase Hydroxymethylglutaryl-CoA reductase kinase protein protein human Homo sapiens Human MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRSCQRSDSDAEAQGKSSEVSLTSSVTSLDSSPVDLTPRPGSHTIEFFEMCANLIKILAQ DIP protein Q13131 original sequence version: 149 DIP protein Q13131 original sequence version: 91 DIP protein Q13131 original sequence version: 165 DIP protein Q13131 original sequence version: 164 ABAE71FBF912947A aapk2_human 5'-AMP-activated protein kinase catalytic subunit alpha-2 PRKAA2 AMPK AMPK2 Acetyl-CoA carboxylase kinase Hydroxymethylglutaryl-CoA reductase kinase protein protein human Homo sapiens Human MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR DIP protein P54646 original sequence version: 146 C46AAFC1D5104975 ppard-prkaa1-1 1 3 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association in AD293 cells cotransfected with Flag-PPARδ and with either catalytic AMPK α1 or α2 subunits, we discovered that each of the AMPK subunits coimmunoprecipitated as a complex with Flag-PPARδ (Figure 5G). Fig. 5G P35396, Q13131 transfection ppard-prkaa2-1 1 4 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association in AD293 cells cotransfected with Flag-PPARδ and with either catalytic AMPK α1 or α2 subunits, we discovered that each of the AMPK subunits coimmunoprecipitated as a complex with Flag-PPARδ (Figure 5G). Fig. 5G P35396, P54646 transfection