UniProt UniProt http://www.uniprot.org Ubxd1 is a novel co-factor of the human p97 ATPase. Ubxd1 is a novel co-factor of the human p97 ATPase. Int. J. Biochem. Cell Biol. (1357-2725) 2008 imex curation Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk 16-Dec-2008: Contacted by JYOTI. HeLa cDNA orchard Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk Accepted 2008-DEC-20 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. HeLa cDNA Int. J. Biochem. Cell Biol. (1357-2725) 2008 orchard Only protein-protein interactions imex curation Ubxd1 is a novel co-factor of the human p97 ATPase. Ubxd1 is a novel co-factor of the human p97 ATPase. Int. J. Biochem. Cell Biol. (1357-2725) 2008 imex curation Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk 16-Dec-2008: Contacted by JYOTI. HeLa cDNA orchard Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk Accepted 2008-DEC-20 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. HeLa cDNA Int. J. Biochem. Cell Biol. (1357-2725) 2008 orchard Only protein-protein interactions imex curation Ubxd1 is a novel co-factor of the human p97 ATPase. Ubxd1 is a novel co-factor of the human p97 ATPase. Int. J. Biochem. Cell Biol. (1357-2725) 2008 imex curation Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk 16-Dec-2008: Contacted by JYOTI. HeLa cDNA orchard Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk Accepted 2008-DEC-20 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. HeLa cDNA Int. J. Biochem. Cell Biol. (1357-2725) 2008 orchard Only protein-protein interactions imex curation Ubxd1 is a novel co-factor of the human p97 ATPase. Ubxd1 is a novel co-factor of the human p97 ATPase. Int. J. Biochem. Cell Biol. (1357-2725) 2008 imex curation Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk 16-Dec-2008: Contacted by JYOTI. HeLa cDNA orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk Accepted 2008-DEC-20 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. HeLa cDNA Int. J. Biochem. Cell Biol. (1357-2725) 2008 orchard Only protein-protein interactions imex curation Ubxd1 is a novel co-factor of the human p97 ATPase. Ubxd1 is a novel co-factor of the human p97 ATPase. Int. J. Biochem. Cell Biol. (1357-2725) 2008 imex curation Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk 16-Dec-2008: Contacted by JYOTI. HeLa cDNA orchard Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Madsen L., Andersen KM., Prag S., Moos T., Semple CA., Seeger M., Hartmann-Petersen R. Pull down was performed in presence of increasing amounts of p47/Nsfl1c rhpetersen@bio.ku.dk Accepted 2008-DEC-20 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. HeLa cDNA Int. J. Biochem. Cell Biol. (1357-2725) 2008 orchard Only protein-protein interactions imex curation tera_human Transitional endoplasmic reticulum ATPase VCP 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein human Homo sapiens Human MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG DIP protein P55072 original sequence version: 89 DIP protein P55072 original sequence version: 114 DIP protein P55072 original sequence version: 123 DIP protein P55072 original sequence version: 150 DIP protein P55072 original sequence version: 174 DIP protein P55072 original sequence version: 175 DIP protein P55072 original sequence version: 176 DIP protein P55072 original sequence version: 160 DIP protein P55072 original sequence version: 128 DIP protein P55072 original sequence version: 132 501B721D3A77BA8A ubxn6_human UBX domain-containing protein 6 UBXN6 UBXD1 UBXDC2 UBX domain-containing protein 1 protein protein human Homo sapiens Human MKKFFQEFKADIKFKSAGPGQKLKESVGEKAHKEKPNQPAPRPPRQGPTNEAQMAAAAALARLEQKQSRAWGPTSQDTIRNQVRKELQAEATVSGSPEAPGTNVVSEPREEGSAHLAVPGVYFTCPLTGATLRKDQRDACIKEAILLHFSTDPVAASIMKIYTFNKDQDRVKLGVDTIAKYLDNIHLHPEEEKYRKIKLQNKVFQERINCLEGTHEFFEAIGFQKVLLPAQDQEDPEEFYVLSETTLAQPQSLERHKEQLLAAEPVRAKLDRQRRVFQPSPLASQFELPGDFFNLTAEEIKREQRLRSEAVERLSVLRTKAMREKEEQRGLRKYNYTLLRVRLPDGCLLQGTFYARERLGAVYGFVREALQSDWLPFELLASGGQKLSEDENLALNECGLVPSALLTFSWDMAVLEDIKAAGAEPDSILKPELLSAIEKLL FC06E6ADA1A1036C nsf1c_rat NSFL1 cofactor p47 Nsfl1c p97 cofactor p47 XY body-associated protein XY40 protein protein rat Rattus norvegicus (Rat) Rat MAEERQDALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEEEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGERRRHSGQDVHVVLKLWKTGFSLDNGDLRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLNTSSPAQQAENEAKASSSILINEAEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFVLMTTFPNKELADENQTLKEANLLNAVIVQRLT 60C81402E5C58134 syvn1_human E3 ubiquitin-protein ligase synoviolin Synovial apoptosis inhibitor 1 SYVN1 HRD1 KIAA1810 RING-type E3 ubiquitin transferase synoviolin protein protein human Homo sapiens Human MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKVMGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLAEDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSILTRGASVQLVFGFEYAILMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHTFPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMVTGAKRLPCNHIFHTSCLRSWFQRQQTCPTCRMDVLRASLPAQSPPPPEPADQGPPPAPHPPPLLPQPPNFPQGLLPPFPPGMFPLWPPMGPFPPVPPPPSSGEAVAPPSTSAAALSRPSGAATTTAAGTSATAASATASGPGSGSAPEAGPAPGFPFPPPWMGMPLPPPFAFPPMPVPPAGFAGLTPEELRALEGHERQHLEARLQSLRNIHTLLDAAMLQINQYLTVLASLGPPRPATSVNSTEETATTVVAAASSTSIPSSEATTPTPGASPPAPEMERPPAPESVGTEEMPEDGEPDAAELRRRRLQKLESPVAH DIP protein Q86TM6 original sequence version: 128 B8A6BACBAF9673C1 herp1_human Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein Methyl methanesulfonate (MMF)-inducible fragment protein 1 HERPUD1 KIAA0025 MIF1 HERP protein protein human Homo sapiens Human MESETEPEPVTLLVKSPNQRHRDLELSGDRGWSVGHLKAHLSRVYPERPRPEDQRLIYSGKLLLDHQCLRDLLPKQEKRHVLHLVCNVKSPSKMPEINAKVAESTEEPAGSNRGQYPEDSSSDGLRQREVLRNLSSPGWENISRPEAAQQAFQGLGPGFSGYTPYGWLQLSWFQQIYARQYYMQYLAATAASGAFVPPPSAQEIPVVSAPAPAPIHNQFPAENQPANQNAAPQVVVNPGANQNLRMNAQGGPIVEEDDEINRDWLDWTYSAATFSVFLSILYFYSSLSRFLMVMGATVVMYLHHVGWFPFRPRPVQNFPNDGPPPDVVNQDPNNNLQEGTDPETEDPNHLPPDRDVLDGEQTSPSFMSTAWLVFKTFFASLLPEGPPAIAN DIP protein Q15011 original sequence version: 131 DIP protein Q15011 original sequence version: 101 3CA827DC7EF0ED22 vcp-ubxn6 4 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association no figure ubxn6-vcp 1 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait pug binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 2b and c VCP/p97 c-terminal synthetic peptide (C-10 peptide) abolished the interaction. Incubation with a phosphorylated C-10 peptide had no effect, suggesting that interacting VCP/p97 is not phosphorylated. P55072 purified ubxn6-vcp-nsfl1c 2 8 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey physical association physical association 2d Nsfl1c was assumed as from rat origin as clones were supplied by H.H. Meyer. P55072; O35987 purified vcp-ubxn6-1 5 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component physical association physical association 3b The collected fractions from the HeLa cell extract separation were analyzed by blotting using antibodies specific for p97 and Ubxd1 as indicated. ubxn6-syvn1 3 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait physical association physical association 3c Q86TM6 transfection ubxn6-herpud1 3 10 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait physical association physical association 3c Q15011 transfection