IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The C-terminal region (640-967) of Arabidopsis CPL1 interacts with the abiotic stress- and ABA-responsive transcription factors. The C-terminal region (640-967) of Arabidopsis CPL1 interacts with the abiotic stress- and ABA-responsive transcription factors. Biochem. Biophys. Res. Commun. (0006-291X) 2008 imex curation Bang WY., Kim SW., Jeong IS., Koiwa H., Bahk JD. jdbahk@gnu.ac.kr 27-Aug-2008: Contacted by JYOTI. Arabidopsis cDNA library jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Bang WY., Kim SW., Jeong IS., Koiwa H., Bahk JD. jdbahk@gnu.ac.kr Accepted 2008-AUG-4 by JYOTI. Arabidopsis cDNA library Biochem. Biophys. Res. Commun. (0006-291X) 2008 jyoti Only protein-protein interactions imex curation The C-terminal region (640-967) of Arabidopsis CPL1 interacts with the abiotic stress- and ABA-responsive transcription factors. The C-terminal region (640-967) of Arabidopsis CPL1 interacts with the abiotic stress- and ABA-responsive transcription factors. Biochem. Biophys. Res. Commun. (0006-291X) 2008 imex curation Bang WY., Kim SW., Jeong IS., Koiwa H., Bahk JD. jdbahk@gnu.ac.kr 27-Aug-2008: Contacted by JYOTI. Arabidopsis cDNA library jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bang WY., Kim SW., Jeong IS., Koiwa H., Bahk JD. jdbahk@gnu.ac.kr Accepted 2008-AUG-4 by JYOTI. 27-Aug-2008: Contacted by JYOTI. Arabidopsis cDNA library Biochem. Biophys. Res. Commun. (0006-291X) 2008 jyoti Only protein-protein interactions imex curation q9lse3_arath At3g26730 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSILPPPIIQGHNSSSPSSDSSLNPSSEHGNNSSSSLSPLPSSPPSRISSSTGTRFSGHSESSHNFSGGSREPSVAAEEGHSRGSSKEMTQNGAGKESFSPPNADKRDHFRYDGRRNRSRGRGTGAAGSSSSQLQHNSGPFNSPRGGVSHHNPTGRRANMISGNHLLNFQYDPISQPQSRGPPPPQRRQQYKGRPFNKDLFLKANYKFVVLDTGDHSPDSMDPDKMLQWDDIICVRYSTPSPVQCPICLEYPLCPQITSCGHIFCFPCILQYLLTGVDNHKVDCFKRCPLCFVMISPRELYTVYIENVKQYSVGDPIEFVLLTRKKDSFAPTRKNEHDLAVPNGENEIYDPFSKFTFTQDVDLSVRQAVSELDSWIARADPQLVEDLEKHLYVNAALERLEQRKLYWNEHKLSIYSKVCTTARNQTQSLSPPDVSRVGYQAPMGGHGAPDSGSNDQDKSTEDSSADKSDGESQSSLEKGCDNDLGHPLEDQDAPSSSSCNGSKGFLSHQIDTKDLKDNDAYNFYQSVDGQHIILHTLNVKCLLHHYGSYDFLPPRLSGKILEMETITQSEAIRRRYRFLSHFSLSTTLQICEIDMKESLPPDAFAPFIHEIKKREKQRKERAWKDRKNKIKAEVAAAAELVPLMASFGQSSSSDDFPPFSLDDFEALGNSAPISSSPPNIGERSSFSHVTRLGFAAGHDSPNLNNEPTNVQSSSSSSEVSNATTGARNTNITSFASVASRTTTPMAPTVKEPGKRGKKQSRVLLSTTSVRRY B42710EEFE868C45 q949m4_arath TRM20 TON1 Recruiting Motif 20 F16A16.130 F16A16_130 At4g28760 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNELRGRKAQKIESPVPGCLGKMVNLFDLGIAVNGNKLLTDKPHLDGSSLSRSRSDVTRMPGPSYKGHSEAELIMSDLRRSASSKLSGTPMKKLIAREMSKEVEHKQSPTNVVAKLMGLETLPQTHQETATQRSKSRSNSHSSLNHSMTSTDNEVQKYQDFSREFKDVYETWQSPQKVSRSRDCSPRKGRYDESTTEKQMALVRQKFSEAKRLVTDDSLHQSKEFQDALEVLSSNKDLFVQFLQESNSFSQQNLSDFHHVPPHSEAKRITVLRPSKAGETEKYVVQGRRNKQVKKLASSSQETGWGNRDLGYPSPYVNRGTEEHTVQPTRIVVLKPSLGKSLDIKAVSSSQSSPRGLHSRGYFDEPEDVETKEVAKEITRQVRENLMGHHRNETQSSSVLSNGYIGDDSSFNKSDNEDLVGNLSDSEIMSPASRHSWDCPNRFDSLFSPSSFSRASFSPESSVCREAKKRLSERWALMSVSGRTQPLKHVSRTSSTLGEMLALTETKVTTESGEGSYEIVPATRVSTSCITSDLSQVEMASDSLNILARSKSVSDVRLNGETSVLGSSKVQAPRELTKTGSLKSSWKVSNLFFFKNNKASKEKRDASQCSSMSQLAAPSPVTLTGKTSEDCVFPIDCLPPVSSEQQSIILGEEEVTTPKPLATGNTSENQDQPSPISVLFPPFEEECASIPECSGSTKHWSSQGDEMSLKSNLIDKSPPIGSIARLLSWDDDSCTDNIAKPAMGVHEEEDWHLFIEMILTAAGFSSGCIVSHDPIMSRWHMPNSPLDPSLRDKYTNPDNNNIKEFIHEGKRRQQRSTRKLIFDRINSIVSETTTTRTGNGSLHFDLVEHVWAQLKDWVSDEPSKRDSGEDMDANSLAAESLVKDEIVGRTWTHSLQVEIDDFGIEIEKRLLQELVEEAVIDLTR 10B55CC87876CA86 q8rwc0_arath At3g54290 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGTCFSSSTKSTAEISPFDLVVKPPPPSAAATAPPTQRIPTAKTETSTVSFTATVRLYGPPNSLVTSYLRFALLHKKVPLRFVPSEDQKPTIQVGSETVSGSREVLLRYIEDKFPEPRLMIWKFNLEGFDEATPLIVKMIWLQHRSMLWHMERMLRWSEDLAARGGKKAVDPSVGTPKMEIRKFAKSYTHLQELMLEHAQMEERILFPVLESVDRGMCKSANEEHGRELPMMNGIKEDIKSIGVLDSGICSEALFSLASRFKSLQMMCKTHFEEEEKDLLPMVEAAEMGKEKQKKLMNQSLEVMSGTHSNSFDFLLEGLTPQEAMQYIDLLMTFGDPNLISSFLCSDVVD DA4E5BCC611D1CC4 myb3_arath Transcription factor MYB3 T22J18.19 At1g22640 MYB3 Myb-related protein 3 F12K8.1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGRSPCCEKAHMNKGAWTKEEDQLLVDYIRKHGEGCWRSLPRAAGLQRCGKSCRLRWMNYLRPDLKRGNFTEEEDELIIKLHSLLGNKWSLIAGRLPGRTDNEIKNYWNTHIKRKLLSRGIDPNSHRLINESVVSPSSLQNDVVETIHLDFSGPVKPEPVREEIGMVNNCESSGTTSEKDYGNEEDWVLNLELSVGPSYRYESTRKVSVVDSAESTRRWGSELFGAHESDAVCLCCRIGLFRNESCRNCRVSDVRTH 132D80DEA58D7D93 nac19_arath NAC domain-containing protein 19 ANAC NAC019 Abscisic-acid-responsive NAC F14G24.16 At1g52890 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGIQETDPLTQLSLPPGFRFYPTDEELMVQYLCRKAAGYDFSLQLIAEIDLYKFDPWVLPNKALFGEKEWYFFSPRDRKYPNGSRPNRVAGSGYWKATGTDKIISTEGQRVGIKKALVFYIGKAPKGTKTNWIMHEYRLIEPSRRNGSTKLDDWVLCRIYKKQSSAQKQVYDNGIANAREFSNNGTSSTTSSSSHFEDVLDSFHQEIDNRNFQFSNPNRISSLRPDLTEQKTGFHGLADTSNFDWASFAGNVEHNNSVPELGMSHVVPNLEYNCGYLKTEEEVESSHGFNNSGELAQKGYGVDSFGYSGQVGGFGFM 167EE787F05E3B45 cpl1_arath RNA polymerase II C-terminal domain phosphatase-like 1 CPL1 FRY2 JOE1 Carboxyl-terminal phosphatase-like 1 Protein JASMONATE OVEREXPRESSING 1 Protein FIERY 2 Protein SHINY 4 SHI4 F17L22.130 At4g21670 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MYSNNRVEVFHGDGRLGELEIYPSRELNQQQDDVMKQRKKKQREVMELAKMGIRISHFSQSGERCPPLAILTTISSCGLCFKLEASPSPAQESLSLFYSSCLRDNKTAVMLLGGEELHLVAMYSENIKNDRPCFWAFSVAPGIYDSCLVMLNLRCLGIVFDLDETLVVANTMRSFEDKIDGFQRRINNEMDPQRLAVIVAEMKRYQDDKNLLKQYIESDQVVENGEVIKVQSEIVPALSDNHQPLVRPLIRLQEKNIILTRINPMIRDTSVLVRMRPSWEELRSYLTAKGRKRFEVYVCTMAERDYALEMWRLLDPEGNLINTNDLLARIVCVKSGFKKSLFNVFLDGTCHPKMALVIDDRLKVWDEKDQPRVHVVPAFAPYYSPQAEAAATPVLCVARNVACGVRGGFFRDFDDSLLPRIAEISYENDAEDIPSPPDVSHYLVSEDDTSGLNGNKDPLSFDGMADTEVERRLKEAISASSAVLPAANIDPRIAAPVQFPMASASSVSVPVPVQVVQQAIQPSAMAFPSIPFQQPQQPTSIAKHLVPSEPSLQSSPAREEGEVPESELDPDTRRRLLILQHGQDTRDPAPSEPSFPQRPPVQAPPSHVQSRNGWFPVEEEMDPAQIRRAVSKEYPLDSEMIHMEKHRPRHPSFFSKIDNSTQSDRMLHENRRPPKESLRRDEQLRSNNNLPDSHPFYGEDASWNQSSSRNSDLDFLPERSVSATETSADVLHGIAIKCGAKVEYKPSLVSSTDLRFSVEAWLSNQKIGEGIGKSRREALHKAAEASIQNLADGYMRANGDPGPSHRDATPFTNENISMGNANALNNQPFARDETALPVSSRPTDPRLEGSMRHTGSITALRELCASEGLEMAFQSQRQLPSDMVHRDELHAQVEIDGRVVGEGVGSTWDEARMQAAERALSSVRSMLGQPLHKRQGSPRSFGGMSNKRLKPDFQRSLQRMPSSGRYS 3FD10F0F3A73154D xb31_arath Putative E3 ubiquitin-protein ligase XBAT31 XBAT31 Ankyrin repeat domain and RING finger-containing protein XBAT31 Protein XB3 homolog 1 RING-type E3 ubiquitin transferase XB31 F8N16.13 At2g28840 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGQSMSCGSRPEHGIFASVQCGDIITIRRVMATEPSLLNQTTPYDRHSVLHVAAANGQIEILSLLLERFTNPDLLNRHKQTPLMLAAMYGRISCVKKLAEVGANILMFDSVNRRTCLHYAAYYGHANCVQAILSAAQSSPVAVHWGYARFVNIRDDKGATPLHLAARQRRPECVNVLLDSGSLVCASTSVYGSPGSTPLHLAARSGSIDCVRKLLAWGADRLQRDASGRIPYVVAMKHKHGACGALLNPSSAEPLVWPSPLKFISELNDEAKLLLEQALMEANREREKTILKGTAYSLPSPSFSDTDDNMSEVSDTELCCICFEQVCTIEVKDCGHQMCAQCTLALCCHNKPNPTTSTVTPPVCPFCRSTIACLVVAQNNNNNNEKSKSLDDVVVVDREAGDVSSSKFRKHRRSINLGEESSSFMGLSTIGSFGRITGRGSGRIAAENELMDKPIL 83C4ACE71A827E72 free1_arath Protein FREE1 FREE1 FYVE domain protein required for endosomal sorting 1 FYVE domain-containing protein 1 FYVE1 T20H2.10 At1g20110 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQQGDYNSYYHHQYSQFQNPTPNPNPNPNPSPPAPATVAGPTDLTRNTYASAPPFTGGYGSADYSNYSQNYTPYGQNSEHVPPSAPSFTSPSQPPPSPPATSLNPNSYSTFNQPPPPPTIHPQPLSSYGSFDSTAPYQQPTSQHMYYSPYDQHQTSGYSSAPPPSSAPAPNPNPAPYSSSLYSAPPYSSGGSSIPPSYEKPSVKFDQSGYDGYNRSRSDLGSDLYGKRSDSGEYPAFEDSYGDGVYAYQGGKVEPYGSRGTAPKSSNSTLFDDYGRSISFSSSGRDSSVSSNSAKIVRAVPKADVQEDSTGGVQKFRVKLLAETYGQTTTDVLCQIGLDGLRMLDPSTSRTLRIYPLENITRCEKLDSSILAFWSKTPVDIEAKRIRLQSNSYTTNTLLDTVTAAMFQAKEIGGSSRPPTSGKLIEQTAEKKKGLGDWMNIIKPVNEEKDHWVPDEAVSKCTSCGSDFGAFIRRHHCRNCGDVFCDKCTQGRIALTAEDNAPQVRVCDRCMAEVSQRLSNAKETTGRNVSLQSHEDLARKLQEEMERNRKSSSGLREGSGRRMKEVACPTCTVHLQVQVPVSGSETIECGVCQNPFLVSAH 919CD7FC2B8E3489 dms3_arath Protein DEFECTIVE IN MERISTEM SILENCING 3 DMS3 IDN1 Protein INVOLVED IN DE NOVO 1 F2K15.110 At3g49250 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MYPTGQQISFQTTPLNVQDPTRMMNLDQSSPVARNETQNGGGIAHAEFAMFNSKRLESDLEAMGNKIKQHEDNLKFLKSQKNKMDEAIVDLQVHMSKLNSSPTPRSENSDNSLQGEDINAQILRHENSAAGVLSLVETLHGAQASQLMLTKGVVGVVAKLGKVNDENLSQILSNYLGTRSMLAVVCRNYESVTALEAYDNHGNIDINAGLHCLGSSIGREIGDSFDAICLENLRPYVGQHIADDLQRRLDLLKPKLPNGECPPGFLGFAVNMIQIDPAYLLCVTSYGYGLRETLFYNLFSRLQVYKTRADMISALPCISDGAVSLDGGIIRKTGIFNLGNRDEVNVRFAKPTASRTMDNYSEAEKKMKELKWKKEKTLEDIKREQVLREHAVFNFGKKKEEFVRCLAQSSCTNQPMNTPR 8DB3D4E3429FEBAE at4g21670-at3g49250 1 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 1 at4g21670-at3g26730 1 8 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey physical association physical association Table 1 at4g21670-at1g20110 1 8 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey physical association physical association Table 1 at4g21670-at4g28760 1 4 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table 1 at4g21670-at3g54290 1 8 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association Table 1 at4g21670-at2g28840 1 8 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey physical association physical association Table 1 at4g21670-at1g22640 1 8 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey physical association physical association Table 1 To identify proteins that interact with the defined C-terminal region (640-967) in AtCPL1, yeast two-hybrid screen was performed by using an AtCPL1640-967 bait clone and an Arabidopsis cDNA prey library. Fifty unique clones were identified as CPL1-interacting proteins after secondary confirmation and sequencing analysis. Among these, eight clones including two transcription factors, ANAC019 (At1g52890) and AtMYB3 (At1g22640), are predicted to be nuclear proteins (Table 1), and exhibited specific interactions with AtCPL1640-967 in yeast two-hybrid assay (Supplementary Fig. S1). at4g21670-at1g52890 1 8 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey physical association physical association Table 1 To identify proteins that interact with the defined C-terminal region (640-967) in AtCPL1, yeast two-hybrid screen was performed by using an AtCPL1640-967 bait clone and an Arabidopsis cDNA prey library. Fifty unique clones were identified as CPL1-interacting proteins after secondary confirmation and sequencing analysis. Among these, eight clones including two transcription factors, ANAC019 (At1g52890) and AtMYB3 (At1g22640), are predicted to be nuclear proteins (Table 1), and exhibited specific interactions with AtCPL1640-967 in yeast two-hybrid assay (Supplementary Fig. S1). at4g21670-at1g20110- 2 8 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 2 at4g21670-at3g26730- 2 3 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Supplementary Figure 2 at4g21670-at3g49250- 2 8 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 2 at4g21670-at4g28760- 2 8 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 2 at4g21670-at3g54290- 2 8 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 2 at4g21670-at2g28840- 2 8 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 2 at4g21670-at1g22640- 2 6 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Supplementary Figure 1 Supplementary Figure 2 Figure 1 Figure 2 The authors stated "The CPL1-MYB3 interaction was observed in the absence of NLS and the second DRM, suggesting the first DRM (727�790) was sufficient for their interaction". The LNL[E/D]L motif of AtMYB3 specifically interacts with AtCPL1640-967 of AtCPL1 at4g21670-at1g52890- 2 8 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey physical association physical association Supplementary Figure 2