IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Rad6-Rad18 mediates a eukaryotic SOS response by ubiquitinating the 9-1-1 checkpoint clamp. Rad6-Rad18 mediates a eukaryotic SOS response by ubiquitinating the 9-1-1 checkpoint clamp. Cell (0092-8674) 2008 imex curation Fu Y., Zhu Y., Zhang K., Yeung M., Durocher D., Xiao W. wei.xiao@usask.ca 08-Jul-2008: Contacted by JYOTI. davet Only protein-protein interactions 6 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Fu Y., Zhu Y., Zhang K., Yeung M., Durocher D., Xiao W. wei.xiao@usask.ca Accepted 2008-JUL-2 by DAVET. 08-Jul-2008: Contacted by JYOTI. Cell (0092-8674) 2008 davet Only protein-protein interactions 6 imex curation Rad6-Rad18 mediates a eukaryotic SOS response by ubiquitinating the 9-1-1 checkpoint clamp. Rad6-Rad18 mediates a eukaryotic SOS response by ubiquitinating the 9-1-1 checkpoint clamp. Cell (0092-8674) 2008 imex curation Fu Y., Zhu Y., Zhang K., Yeung M., Durocher D., Xiao W. wei.xiao@usask.ca 08-Jul-2008: Contacted by JYOTI. davet Only protein-protein interactions 6 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Fu Y., Zhu Y., Zhang K., Yeung M., Durocher D., Xiao W. wei.xiao@usask.ca Accepted 2008-JUL-2 by DAVET. 08-Jul-2008: Contacted by JYOTI. Cell (0092-8674) 2008 davet Only protein-protein interactions 6 imex curation pcna_yeast Proliferating cell nuclear antigen YBR0811 YBR088C POL30 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLEAKFEEASLFKRIIDGFKDCVQLVNFQCKEDGIIAQAVDDSRVLLVSLEIGVEAFQEYRCDHPVTLGMDLTSLSKILRCGNNTDTLTLIADNTPDSIILLFEDTKKDRIAEYSLKLMDIDADFLKIEELQYDSTLSLPSSEFSKIVRDLSQLSDSINIMITKETIKFVADGDIGSGSVIIKPFVDMEHPETSIKLEMDQPVDLTFGAKYLLDIIKGSSLSDRVGIRLSSEAPALFQFDLKSGFLQFFLAPKFNDEE 9B56C5851621DDDC 9B56C5851621DDDC ubiq_yeast Ubiquitin UBI1 RPL40A YIL148W UBI2 RPL40B YKR094C UBI3 RPS31 YLR167W L9470.14 UBI4 SCD2 YLL039C protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG obsolete protein 9AC936269D38403E rad18_yeast Postreplication repair E3 ubiquitin-protein ligase RAD18 RAD18 YCR066W YCR66W Radiation sensitivity protein 18 RING-type E3 ubiquitin transferase RAD18 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDHQITTASDFTTTSIPSLYQLDTLLRCHICKDFLKVPVLTPCGHTFCSLCIRTHLNNQPNCPLCLFEFRESLLRSEFLVSEIIQSYTSLRSSLLDALRIPKPTPVPENEEVPGPENSSWIELISESESDSVNAADDDLQIVATSERKLAKRSMTDILPLSSKPSKRNFAMFRSERIKKKSKPNEQMAQCPICQQFYPLKALEKTHLDECLTLQSLGKKPKISTTFPTESNPHNKSSSRFKVRTPEVDKSSCGETSHVDKYLNSMMSAEHQRLPKINFTSMTQSQIKQKLSSLGLSTNGTRQNMIKRYNHYEMLWNSNFCDSLEPVDEAELKRQLLSWDVSHNKTPQNSSNKGGISKLMIMKSNGKSSSYRKLLENFKNDKFNRKGWMVMFRKDFARLIREAKMKIKTGSSDSSGSVGHSNDGDGVEKVQSDQGTEDQQMEKDQDTVINEDRVAGERNLPNEDSTDADLSRELMDLNEYSKDPPGNN 70F5F12A3FA99532 70F5F12A3FA99532 rad17_yeast DNA damage checkpoint control protein RAD17 YOR368W RAD17 DNA repair exonuclease RAD17 protein protein yeast Saccharomyces cerevisiae Baker's yeast MRINSELANKFSASTVHLEHITTALSCLTPFGSKDDVLIFIDADGLSFVRENNHVIKIQLLLSRELFMSYSYRNETEDHMKLCVKINHILDSVSVMNRNSDDIVECTLSYDGHGSPFVLIFEDSFISERVEYSTYLIKDFDTNGLELDRERISFEAIIKGEALHSALKDLKEIGCKECYVYAKTEANDENVFALISKSQLGFSKIKLPSNRSILEKLQVFDGDSTTVIDGFAVIGFFDFTSFDKIRKSTKIASKVLFRMDVHGVLSVNILSQTDDVIITDTTRPSNNRPGSIRQLQLPKDYPGIVIEVCMLEKESIDEAAQTEIELLMETNELGNRNSFKKSTIRKRYGTDKGNETSNDNLLQLNGKKIKLPSEEENNKNRESEDEENHCKYPTKDIPIFF BFF7074676C483C9 BFF7074676C483C9 ddc1_yeast DNA damage checkpoint protein 1 DDC1 YPL194W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFKATITESGKQNIWFRAIYVLSTIQDDIKITVTTNELIAWSMNETDTTLCQVRFQKSFFEEYEFKPHEIVFGENGVQVIEDTYGNSHKLYSFRVNGRHLTTISRKPDGDGIKSFTIAVNNTSTCPESLANRLIVVIEMDSLIVKEYCPQFQPIKYDPIIINLKYKRRFLDVFGTAASDRNPQEPLDPKLLDVFTNTERELTSALFNEEVESDIRKRNQLTAADEINYICCNSTLLKNFLDNCNVNVTDEVKLEINVHRLSITAFTKAVYGKNNDLLRNALSMSNTISTLDLEHYCLFTTIEDEKQDKRSHSKRREHMKSIIFKLKDFKNFITIGPSWKTTQDGNDNISLWFCHPGDPILMQMQKPGVKLELVEVTDSNINDDILEGKFIKTAISGSKEEAGLKDNKESCESPLKSKTALKRENLPHSVAGTRNSPLKVSYLTPDNGSTVAKTYRNNTARKLFVEEQSQSTNYEQDKRFRQASSVHMNMNREQSFDIGTTHEVACPRNESNSLKRSIADICNETEDPTQQSTFAKRADTTVTWGKALPAADDEVSCSNIDRKGMLKKEKLKHMQGLLNSQNDTSNHKKQDNKEMEDGLGLTQVEKPRGIFD F9933697C61021D3 F9933697C61021D3 mec3_yeast DNA damage checkpoint control protein MEC3 MEC3 PIP3 YLR288C L8003.15 PSO9 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKLKLIVNGCEAPDDYKLLRTTINTVASLRKTAILRFNSERLTIISTPKSSLNSSNNGTILRGDTGQLWCTIPHDVFRLYTVISARELNTITMECNCDSLLSVFKRYDRVMNQGSSSNMTIKLQSMPEWNTNNGTLSGGTAGGVDTTSKPNPICALGITFEEIVHTSGPNDAIVMNGGVDEHNGLPTTVGTGNLLASNKVIMHSFKVPVKLLFRAQDTRIQEPMINYIQLMMYKLPPISGEFGSAFHGFIRRVERYSNVNHIHLMGVKKKEHGNEGDDVELKIIVNELDWHLEICWNGPLDSVIQRQEGLTDNPSQNQHIDTDGRQEEGSLPIIEADKPMSSLYTNTRDREMEENIRYDEDLLRIEDSSIADTRGNIYTADTSGDTEFNDISVMVEKAEQESSSTHEVIIRCKDWKVCSKLYAAFEEVVLAISHDESCVFHCSLDRGSLEDSEDVEKPRERGQIIYYIARSKGL 4385CEFA9130FEA3 4385CEFA9130FEA3 ubc2_yeast Ubiquitin-conjugating enzyme E2 2 RAD6 UBC2 Ubiquitin-protein ligase UBC2 Ubiquitin carrier protein UBC2 Radiation sensitivity protein 6 YGL058W E2 ubiquitin-conjugating enzyme 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTPARRRLMRDFKRMKEDAPPGVSASPLPDNVMVWNAMIIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKETVEKSWEDDMDDMDDDDDDDDDDDDDEAD 5F568DC28ABBD60F 5F568DC28ABBD60F rad17-rad18 1 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 4 B only Rad17 directly interacts with Rad18 pol30-rad18 1 5 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association 4 B the level of interaction is comparable to that of Pol30 with Rad18 mec3-rad17-1 1 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 4 B ddc1-rad17 1 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 4 B rad6-rad18-1 1 9 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 4 B ubl1-rad17 2 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4 E Methyl methane-sulfonate (MMS) - MMS treatment of cells induced this interaction. the anti-Ub antibody precipitated a protein that can be detected by the anti-Myc antibody and comigrates with the modified Rad17-Myc band from the whole-cell extract