IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Tyrosine kinases Btk and Tec regulate osteoclast differentiation by linking RANK and ITAM signals. Tyrosine kinases Btk and Tec regulate osteoclast differentiation by linking RANK and ITAM signals. Cell (0092-8674) 2008 imex curation Shinohara M., Koga T., Okamoto K., Sakaguchi S., Arai K., Yasuda H., Takai T., Kodama T., Morio T., Geha RS., Kitamura D., Kurosaki T., Ellmeier W., Takayanagi H. Apoptosis - Interactions involving proteins with a function related to apoptosis taka.csi@tmd.ac.jp 16-April-2008: Contacted by JYOTI. Only protein-protein interactions 2 mouse-bmm mouse bone marrow derived macrophages bmm bone marrow derived macrophages Bone marrow derived macrophages (BMM) were generated in vitro from precursor cells harvested from the femurs of C57BL/6 mice (7�8 weeks) by proliferation and differentiation in the presence of L-cell conditioned medium. as a source of CSF-1 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Shinohara M., Koga T., Okamoto K., Sakaguchi S., Arai K., Yasuda H., Takai T., Kodama T., Morio T., Geha RS., Kitamura D., Kurosaki T., Ellmeier W., Takayanagi H. Apoptosis - Interactions involving proteins with a function related to apoptosis taka.csi@tmd.ac.jp Accepted 2008-APR-19 by CDEROW. Cell (0092-8674) 2008 Only protein-protein interactions 2 imex curation Tyrosine kinases Btk and Tec regulate osteoclast differentiation by linking RANK and ITAM signals. Tyrosine kinases Btk and Tec regulate osteoclast differentiation by linking RANK and ITAM signals. Cell (0092-8674) 2008 imex curation Shinohara M., Koga T., Okamoto K., Sakaguchi S., Arai K., Yasuda H., Takai T., Kodama T., Morio T., Geha RS., Kitamura D., Kurosaki T., Ellmeier W., Takayanagi H. Apoptosis - Interactions involving proteins with a function related to apoptosis taka.csi@tmd.ac.jp 16-April-2008: Contacted by JYOTI. Only protein-protein interactions 2 mouse-bmm mouse bone marrow derived macrophages bmm bone marrow derived macrophages Bone marrow derived macrophages (BMM) were generated in vitro from precursor cells harvested from the femurs of C57BL/6 mice (7�8 weeks) by proliferation and differentiation in the presence of L-cell conditioned medium. as a source of CSF-1 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shinohara M., Koga T., Okamoto K., Sakaguchi S., Arai K., Yasuda H., Takai T., Kodama T., Morio T., Geha RS., Kitamura D., Kurosaki T., Ellmeier W., Takayanagi H. Apoptosis - Interactions involving proteins with a function related to apoptosis taka.csi@tmd.ac.jp Accepted 2008-APR-19 by CDEROW. 16-April-2008: Contacted by JYOTI. Cell (0092-8674) 2008 Only protein-protein interactions 2 imex curation btk_mouse Tyrosine-protein kinase BTK Bruton tyrosine kinase B-cell progenitor kinase Kinase EMB Btk Bpk Agammaglobulinemia tyrosine kinase protein protein mouse Mus musculus Mouse MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVIPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPTAAPISTTELKKVVALYDYMPMNANDLQLRKGEEYFILEESNLPWWRARDKNGQEGYIPSNYITEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGEPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSKQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIREGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASERVYTIMYSCWHEKADERPSFKILLSNILDVMDEES E502B798BC36E223 blnk_mouse B-cell linker protein Cytoplasmic adapter protein B-cell adapter containing a SH2 domain protein B-cell adapter containing a Src homology 2 domain protein Src homology 2 domain-containing leukocyte protein of 65 kDa Lymphocyte antigen 57 Blnk Bash Ly57 Slp65 protein protein mouse Mus musculus Mouse MDKLNKITVPASQKLRQLQKMVHDIKNNEGGIMDKIKKLKVKGPPSVPRRDYALDSPADEEEQWSDDFDSDYENPDEHSDSEMYVMPAEETGDDSYEPPPAEQQTRVVHPALPFTRGEYVDNRSSQRHSPPFSKTLPSKPSWPSAKARLASTLPAPNSLQKPQVPPKPKDLLEDEADYVVPVEDNDENYIHPRESSPPPAEKAPMVNRSTKPNSSSKHMSPPGTVAGRNSGVWDSKSSLPAAPSPLPRAGKKPATPLKTTPVPPLPNASNVCEEKPVPAERHRGSSHRQDTVQSPVFPPTQKPVHQKPVPLPRFPEAGSPAADGPFHSFPFNSTFADQEAELLGKPWYAGACDRKSAEEALHRSNKDGSFLIRKSSGHDSKQPYTLVAFFNKRVYNIPVRFIEATKQYALGKKKNGEEYFGSVVEIVNSHQHNPLVLIDSQNNTKDSTRLKYAVKVS 66C93D4FDDF9D260 btk-blnk-1 1 3 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component colocalization colocalization Immunofluorescence microscopic analysis of colocalization of Btk and BLNK Tnfsf11/Rankl Fig. 5A and Supp. Fig. 3 Colocalisation not observed in DAP12-/-FcRgamma-/- cells. btk-blnk-2 2 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association immunoblot analysis showed that Btk coimmunoprecipitated with BLNK in BMMs in the presence of RANKL, but the association was abrogated in DAP12−/−FcRgamma−/− cells (Figure 5B). Tnfsf11/Rankl Fig. 5B Interaction not seen in DAP12-/-FcRgamma-/- cells or Tec-/-Btk-/- cells.