IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Arabidopsis PCFS4, a homologue of yeast polyadenylation factor Pcf11p, regulates FCA alternative processing and promotes flowering time. Arabidopsis PCFS4, a homologue of yeast polyadenylation factor Pcf11p, regulates FCA alternative processing and promotes flowering time. Plant J. (0960-7412) 2008 Xing D., Zhao H., Xu R., Li QQ. liq@muohio.edu 27-Aug-2008: Contacted by JYOTI. jyoti arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. tap tandem affinity purification TAP TAP tap western blot western blot Immuno blot Immuno blot Xing D., Zhao H., Xu R., Li QQ. liq@muohio.edu Accepted 2008-SEP-13 by JYOTI. 27-Aug-2008: Contacted by JYOTI. Plant J. (0960-7412) 2008 jyoti Arabidopsis PCFS4, a homologue of yeast polyadenylation factor Pcf11p, regulates FCA alternative processing and promotes flowering time. Arabidopsis PCFS4, a homologue of yeast polyadenylation factor Pcf11p, regulates FCA alternative processing and promotes flowering time. Plant J. (0960-7412) 2008 Xing D., Zhao H., Xu R., Li QQ. liq@muohio.edu 27-Aug-2008: Contacted by JYOTI. jyoti yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Xing D., Zhao H., Xu R., Li QQ. liq@muohio.edu Accepted 2008-SEP-13 by JYOTI. Plant J. (0960-7412) 2008 jyoti fy_arath Flowering time control protein FY FY T6I14.10 At5g13480 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MYAGGDMHRGSQMPQPPMMRQSSASSTNINPDYHHPSGPFDPNVDSFGAKRMRKHTQRRAVDYTSTVVRYIQARTWQRDSRDRTTLQPTPAAAVDMLPTVAYSDNPSTSFAAKFVHASLNKNRCSINRVLWTPSGRRLITGSQSGEFTLWNGQSFNFEMILQAHDQPIRSMVWSHNENYMVSGDDGGTLKYWQNNMNNVKANKTAHKESIRDLSFCKTDLKFCSCSDDTTVKVWDFTKCVDESSLTGHGWDVKSVDWHPTKSLLVSGGKDQLVKLWDTRSGRELCSLHGHKNIVLSVKWNQNGNWLLTASKDQIIKLYDIRTMKELQSFRGHTKDVTSLAWHPCHEEYFVSGSSDGSICHWIVGHENPQIEIPNAHDNSVWDLAWHPIGYLLCSGSNDHTTKFWCRNRPADNPRDVLMQNQGYNEQGFGRQPDNFQPSEASPIPGAFVPGLTRNEGTIPGIGIAMPFDASSQGDHKQPLPGSMALGAPPLPPGPHPSLLGSGQQQGYQQQQQHQGHPQQMLPMPNMPHHQLPPSSHMPLHPHHLPRPMQMPPHGHMPPPSMPMSHQMPGSMGMQGGMNPQMSQSHFMGAPSGVFQGQPNSGGPQMYPQGRGGFNRPQMIPGYNNPFQQQQQPPLPPGPPPNNNQQHQ DIP protein Q6NLV4 original sequence version: 79 74569D2178BEA5C7 clp1_arath Protein CLP1 homolog CLPS3 CLP-like protein 3 Protein CLP-SIMILAR PROTEIN 3 F7O18.15 At3g04680 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAYGGPSMNPPALSGAVPGSANLKQVKLERESELRIEVSEEPLRLRVVNGTAEIFGSELPPEIWRTFPPRMKFAVFTWYGATIEMDGVTETDYTADETPMVSYINVHAILDARRRFAKASTSNDPESSQGPRVIVVGPTDSGKSTLTKMLLSWAAKQGWRPTFVDLDVGQGSITIPGSIAAAPIEMPLDPVEGFPLDMALVYYYGHASPNMNVELYKALVKELAQVLEKQFVGNPESRAAGMVINTMGWIEGIGYELLLHAIDTFNASVVLVLGQEKLFSRLKDVLRSKSNVDVVKLHKSGGVVARVKEVRKRSRNFKIQEYFYGLSKELSPYANTSSFSDLQVFRIGGGPQAPKSALPAGSTSVSNPLRVTPVNIDDRDLLHSVLAVSYAEEPDQIISSNVSGFVYVTEVNVQKKKITYLAPSPGTLPSKLLVAGSLAWLESV 04968918D3A539AA pcfs4_arath Polyadenylation and cleavage factor homolog 4 PCFS4 T4B21.1 At4g04885 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDSEKILNPRLVSINSTSRKGMSVELPQKPPPPPSLLDRFKALLNQREDEFGGGEEVLPPSMDEIVQLYEVVLGELTFNSKPIITDLTIIAGEQREHGEGIANAICTRILEAPVEQKLPSLYLLDSIVKNIGRDYGRYFSSRLPEVFCLAYRQAHPSLHPSMRHLFGTWSSVFPPPVLRKIDMQLQLSSAANQSSVGASEPSQPTRGIHVNPKYLRRLEPSAAENNLRGINSSARVYGQNSLGGYNDFEDQLESPSSLSSTPDGFTRRSNDGANPSNQAFNYGMGRATSRDDEHMEWRRKENLGQGNDHERPRALIDAYGVDTSKHVTINKPIRDMNGMHSKMVTPWQNTEEEEFDWEDMSPTLDRSRAGEFLRSSVPALGSVRARPRVGNTSDFHLDSDIKNGVSHQLRENWSLSQNYPHTSNRVDTRAGKDLKVLASSVGLVSSNSEFGAPPFDSIQDVNSRFGRALPDGTWPHLSARGPNSLPVPSAHLHHLANPGNAMSNRLQGKPLYRPENQVSQSHLNDMTQQNQMLVNYLPSSSAMAPRPMQSLLTHVSHGYPPHGSTIRPSLSIQGGEAMHPLSSGVLSQIGASNQPPGGAFSGLIGSLMAQGLISLNNQPAGQGPLGLEFDADMLKIRNESAISALYGDLPRQCTTCGLRFKCQEEHSKHMDWHVTKNRMSKNHKQNPSRKWFVSASMWLSGAEALGAEAVPGFLPTEPTTEKKDDEDMAVPADEDQTSCALCGEPFEDFYSDETEEWMYKGAVYMNAPEESTTDMDKSQLGPIVHAKCRPESNGGDMEEGSQRKKMRS 03BABA1EE2B43515 at4g04885-at3g04680 2 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Figure 5a However, we found that PCFS4 did interact with another putative polyadenylation factor, CLp1p-similar protein 3 at4g04885-at3g04680- 1 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey association association Figure 5b Arabidopsis Pcf11p-similar protein 4 (PCFS4) forms a protein complex with FY and CLP1p-similar protein 3 (CLPS3)