UniProt UniProt http://www.uniprot.org A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination. A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination. Dev. Cell (1534-5807) 2007 imex curation Starostina NG., Lim JM., Schvarzstein M., Wells L., Spence AM., Kipreos ET. Apoptosis - Interactions involving proteins with a function related to apoptosis ekipreos@cb.uga.edu 16-Oct-2008: Contacted by JYOTI. davet Only protein-protein interactions caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Starostina NG., Lim JM., Schvarzstein M., Wells L., Spence AM., Kipreos ET. Apoptosis - Interactions involving proteins with a function related to apoptosis ekipreos@cb.uga.edu Accepted 2008-OCT-30 by DAVET. 16-Oct-2008: Contacted by JYOTI. Dev. Cell (1534-5807) 2007 davet Only protein-protein interactions imex curation A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination. A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination. Dev. Cell (1534-5807) 2007 imex curation Starostina NG., Lim JM., Schvarzstein M., Wells L., Spence AM., Kipreos ET. Apoptosis - Interactions involving proteins with a function related to apoptosis ekipreos@cb.uga.edu 16-Oct-2008: Contacted by JYOTI. davet Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Starostina NG., Lim JM., Schvarzstein M., Wells L., Spence AM., Kipreos ET. Apoptosis - Interactions involving proteins with a function related to apoptosis ekipreos@cb.uga.edu Accepted 2008-OCT-30 by DAVET. 16-Oct-2008: Contacted by JYOTI. Dev. Cell (1534-5807) 2007 davet Only protein-protein interactions imex curation A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination. A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination. Dev. Cell (1534-5807) 2007 imex curation Starostina NG., Lim JM., Schvarzstein M., Wells L., Spence AM., Kipreos ET. Apoptosis - Interactions involving proteins with a function related to apoptosis ekipreos@cb.uga.edu 16-Oct-2008: Contacted by JYOTI. davet Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Starostina NG., Lim JM., Schvarzstein M., Wells L., Spence AM., Kipreos ET. Apoptosis - Interactions involving proteins with a function related to apoptosis ekipreos@cb.uga.edu Accepted 2008-OCT-30 by DAVET. 16-Oct-2008: Contacted by JYOTI. Dev. Cell (1534-5807) 2007 davet Only protein-protein interactions imex curation A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination. A CUL-2 ubiquitin ligase containing three FEM proteins degrades TRA-1 to regulate C. elegans sex determination. Dev. Cell (1534-5807) 2007 imex curation Starostina NG., Lim JM., Schvarzstein M., Wells L., Spence AM., Kipreos ET. Apoptosis - Interactions involving proteins with a function related to apoptosis ekipreos@cb.uga.edu 16-Oct-2008: Contacted by JYOTI. davet Only protein-protein interactions caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Starostina NG., Lim JM., Schvarzstein M., Wells L., Spence AM., Kipreos ET. Apoptosis - Interactions involving proteins with a function related to apoptosis ekipreos@cb.uga.edu Accepted 2008-OCT-30 by DAVET. 16-Oct-2008: Contacted by JYOTI. Dev. Cell (1534-5807) 2007 davet Only protein-protein interactions imex curation zer1_caeel Zyg eleven-related protein 1 zer-1 zyg-11-related protein T24C4.6 protein protein caeel Caenorhabditis elegans MSHTADIPLEAPTLLKLASSSVQSQINNDEYMETVILPVPLSQELFARFRNKWNIHNHGPAPVEHLGPPTDEPRPDQGNIDMIKQVFRIWSDSTRLPLEKLNLTGATVDDQIFVDLLHAHAHSITELDLTNVTGVTDLSNAYLYARSTHFPMLTSIRMTSMDLVTGHQPRRKNGIASTEFRALFMLGEEALAEARGQMERDGLRSPLSPSSQPSSIQSDHQSLDALVPCDSPVEPPLITARAPNVKRLFLPRCPAKRTTIDEEEQNTHVILTKILSPLQQLEVLDLSYWSKTDDMRCLQPLSNTLTCLILYDVPDLYHAVPNICHMTELRILDVSQSNRDSGLYPHPVTTLNKLIVSLKYLTHLDISSTNLATQPSSHDNPARYRESVRTDICGLQSLVRPLKYLGLFNCESASHVREIPAEVVSGDANEDQVITSLQMYKDRAGLLQNVLNESYQLYRFGNSNPLTRHTEALHLVLEAMHRHLADSTLQIAGSASLFYIIRKVDMNRDTKRRVVSALLSGMEVHMEEQVMVRNCCLSLCQFEIPQDILFDYSRLAVLLVSVLQHHNADNLTQRIVVFLLNSMACHVEGDQKVQVGSYGAIEMILDQIARKHTANVCDDVMEVGWSFLWNITDETPVNCELFLNANGLDLFQKCYEAFKTERELVRNMMGLIGNIAEVDELRSQLMKDDYVKIFCALLESQEESIEISYNSAGVLAHMVSDGEEVWKQMTVCRNEVMQRIVEATSSWKLATRRFINYRSFRPILRLLPLYHAYASQHWAVWALANLTTTDGEKYCAYVRDEGGVPLLEELVSNAITTTDIRTLANTVLENIRNVENKRNPKSIPADLAHSSNSPTFVPSYVSDVEEEDEGDVEADEEVQDFVNVDFDDYALPMEY 9713C1B260C884C3 fem2_caeel Protein phosphatase fem-2 Ca(2+)/calmodulin-dependent protein kinase phosphatase fem-2 Sex-determining protein fem-2 Feminization of XX and XO animals protein 2 T19C3.8 protein protein caeel Caenorhabditis elegans MEKVNEERDAVFEDHIGDRRRSVRSLLEEAFADEMEKTSYDVEVADTPQPHIPIRFRHPPIAGPVHDVFGDAIHDIFQKMMKRGQAVDFCHWVSHLIATEIDEKFSEVAFRDVQYNPDIYVTDSTTEAKKLFNDKIWPAIDKILQQNAETCPILSEKWSGIHVSGDQLKGQRHKQEDRFLAYPNGQYMDRGEDPISVLAVFDGHGGHECSQYAAGHLWETWLEVRKSRDPSDSLEDQLRKSLELLDERMTVRSVKECWKGGSTAVCCAIDMDQKLMALAWLGDSPGYVMSNIEFRQLTRGHSPSDEREARRVEEAGGQLFVIGGELRVNGVLNLTRALGDVPGRPMISNEPETCQVPIESSDYLVLLACDGISDVFNERDLYQLVEAFANDYPVEDYAELSRFICTKAIEAGSADNVSVVIGFLRPPQDVWKLMKHESDDEDSDVTDEE E51705B3DA0FF49D g5ecr7_caeel 3L686 elb-1 Y41C4A.10 CELE_Y41C4A.10 protein protein caeel Caenorhabditis elegans MSQQELFFEVRRNRSHIYLDAEESHSVMELKLMLAGITRDPVNHMELWKLDEDGNKSQLLHDTATLSDAGFSSTNAKAQCPAALGLRLTNAEEHLTISDVSTAPPIPDTMRQEQAPQE 3CD19CB032815D6A vhl_caeel von Hippel-Lindau tumor suppressor homolog vhl-1 F08G12.4 protein protein caeel Caenorhabditis elegans MSDGSMDDDGRLFPDLGSSTHDNREIRVRFLNRCAYPVDVFWLNPSKQPTKYGTLAQKKYLDIKTFKDHPWVARRSFDGCKVLVNEKEVFWPEPAPRMNLIVRNHCVITMKVQSLREIAGRSFLRHNPTEVPNKIKGLPRELQFEVKHFLDRKQEYSEIVCRSIPPPGPQRPQQ A082698EF932EAC6 zyg11_caeel Early embryogenesis protein zyg-11 zyg-11 C08B11.1 protein protein caeel Caenorhabditis elegans MDHNAALTTCSTSAIPRLARLATERIAELIENDALPEFDHKVIASCSNDIFEVLRQKKKLNHQTLQTMCSTSRFQINKIDLMGNKVELQDLELCSNQNLVSFRLGDIDYFPNTNKIQVADVLDTALNRTSRQQLRHLDLSGRHRITSNWPTYVARKLPRLESLAFANRSTANETLSQIGASLLNLRFLDISSTCVTDISCISSLRNLEVLIMYNLNILKGDVTETLSNLTKLRVLDISRKVNTDYLQETSQDAHLDLALGIYNRSVEAIESGTATPWAELRAIDMSGLSIVQFGTDRALAFVEKIIEAHPKLEQISLLATPLDSSLIEIPNRNLQVINTVSRRSIIFALSHYANLDRPAFITHALHSVYYQLQSGYDKFSQEELKECLRLVCISMQQGLNTLPVQIAGSACLYHLCKMKRIKRLSVKEVNNCIERSLDAAEQYRSMTQLQKNVWLTICNDYLLHLDEIDFYRTCKVALDTMLLNRDASVERMTIAIVSIVTPKMRPSEAKILTTETKYVYHLVKIMNDYLEAYTREHRVGHERDNENALYTLKFTLSALWNLTDECPATCKAFLDAGGVQIAFRILKAFDYHGNVQTKVLGILNNLAEVEELHLGQLCKNEYISVLISCLDGSFNEVDSKGRYREVERSYFAAGILANLLTNTDGWESENQRDEACEKLLELIEQYPTLPSAMVSYKSFIPFSRIVRESNSNGAIMWCLWGVHHVLQHREKNKAPTYEKGYIEMFMDSGLSPIVEDMSHGHSKHIHNLDIRVVHLAREIIDIVSCHSLSSSPVRLVRRV B216E54C8F6374BD fem3_caeel Sex-determination protein fem-3 Feminization of XX and XO animals protein 3 Ce-FEM-3 C01F6.4 fem-3 protein protein caeel Caenorhabditis elegans MEVDPGSDDVEADRETRAQKLKLKRNVKFRAQMRRFDEYCGVTNLTVDDLNWPLISGIPLQRQRLTGATYYDDSLLDQNPWDEFSIDRFLEITSIQLITAGAGYERNDEITRFVFQRTMKTIVTYCNFMYDLARRNGKVQITRFELQDLIHRDEFRFYMYFRQFLPNPDPNCTAFSNHYTSLLHTLYFNIPGMPQFWNNSQMYNYAATRGQRLVQNIAAFYPPEYFWNEDESKYHTTFVVPRGTEFSKFYARRFHEALGMPPLENEIITVLDWLAKLCILEIVYHTTIWCDITGFGGLPRIEHYRLAMENVEDIIFDLAIDDFSISRLQLQISPFEISRYSPLDVSGYYETIKRKKDIEEYQNRFYEVHYSDDVRIMNVYATDCSRKR 5366B23BBCF0CA28 cul2_human Cullin-2 CUL2 protein protein human Homo sapiens Human MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA DIP protein Q13617 original sequence version: 129 DIP protein Q13617 original sequence version: 127 DIP protein Q13617-1 original sequence version: 139 DIP protein Q13617 original sequence version: 111 DIP protein Q13617 original sequence version: 102 30647248F671AB0E q9bks1_caeel Elongin-C elc-1 Y82E9BR.15 CELE_Y82E9BR.15 protein protein caeel Caenorhabditis elegans MADQNNAIQCDQDAAQPKQYGGIEGPTSQYVKLVSSDDHEFIIKRELALTSGTIRAMLSGPGVYAENESNVVYFREIPSHVLQKVCQYFAYKVRYTHAATEIPEFPIPPDVALELLMAANFLDC F14A3E98A0C9964E rbx1_caeel RING-box protein 1 rbx-1 Ce-rbx-1 ZK287.5 protein protein caeel Caenorhabditis elegans MAQASDSTAMEVEEATNQTVKKRFEVKKWSAVALWAWDIQVDNCAICRNHIMDLCIECQANQAAGLKDECTVAWGNCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH EFE50F7684B30A56 fem1_caeel Sex-determining protein fem-1 fem-1 isx-1 Feminization of XX and XO animals protein 1 F35D6.1 protein protein caeel Caenorhabditis elegans MTPNGHHFRTVIYNAAAVGNLQRIKVFTINSRNDRQWIIDCFNSDQDGRYPLVIAARNGHANVVEYLLEIGADPSVRGVVEFDNENIQGTPPLWAASAAGHIEIVKLLIEKANADVNQATNTRSTPLRGACYDGHLDIVKYLLEKGADPHIPNRHGHTCLMIASYRNKVGIVEELLKTGIDVNKKTERGNTALHDAAESGNVEVVKILLKHGSVLMKDIQGVDPLMGAALSGFLDVLNVLADQMPSGIHKRDALKLLGSTLLDKKMDAMSAMNCWKQSMEVPLHADDLRLVREMETFFEPQEVYEYQREAQNIAQVELLDGNIEAQRMQALVIRERILGGAHTDVHYYLRFRGAVYCDMGQMNRCYDLWKHALELQQKHFAPLYYGTITTLQSFHETFSMSLNDFVNNHHANRNLRVRSSWVKYVFNGVCLELERAAAWTGAPLLEDTECCGKERCQHATEESEYKKLVYVAVHLVNVLERLSLPSAHIDDSDEEKEPKADVRRLMIVCHELHIPLLHHTLEERIPDSNSAELGLPKAAVLEQLLELDLDVNATDKNNDTPMHILLRAREFRKSLVRALLVRGTWLFARNRHGDVVLNVMKRMKALNHANFDDLPLGRHITLAGLVANAMRVKYPKKFVGVEEQMPLELRRFYLAH 4F5FC3FA3EA950D3 cand1_caeel Cullin-associated NEDD8-dissociated protein 1 cand-1 Cullin-associated and neddylation-dissociated protein 1 Y102A5A.1 protein protein caeel Caenorhabditis elegans MSAYHVGQLVDKMSNPDKDFRFMACNDLMKDLQTGTIALEDDSTAKVIRALIKLLSDSNGEVQNLAIKCIGLLAQPSKIKTHHLEYLVEELTPHVFSKAEQSRDIHSLTLKAMILNLAPSASSNATTTVVKRMLPKFVDSLSLCAPDDAARVDVLDLIGEVLLRFGDVVPEMHKGSLKVMVDHLYSFRSAIRKKAITGIGHLASVINGELYDELVQDLLKELAQRSPPSSAAQNVQLRTLVIALSTVARASGSRFSKHTPKVVPFLLQYLQIDPGTESEHDDLREASIQGLEVFLYRNPQEVVAFEKEVIQQLTDALAYDPNYEYGDDDEDEQMEDDEDDDEDEYSDDEDVTWKVRRAAAKAIEAMISSHRESLLNLSQKIGPVVIGRFKEREETVRTEIISVYIALLNQISILVPDLQKAVVAADEDSIETDDIVVIGGTKFSTNYLSRSQLAIIQSLADQKDVLLRTITKSMKKHPKTGPKCIELLSALIRTYPSGLEDSLDDIIPAVSNILTDKNASAQGKMTVLSFISNALTLNNPKRFKNLLSPLTTIMTHSISEPFYKVSAEGLAVCCKYIDVLRELSACGGNEEAKKLLVVVEKKFMANDTDQEVRERAISAISMLLAAFKDVLKNETPAILEKMTERIGRDMTCLVAFRASTHIVEAGIIFSSAQLQSILRHVVDYVKKIARSLRMTCLNFVEKLMKHSPAGSIPVEELTCVLGEMSNLISETDLQITNQAFCCLTYAFLNFPTCVSLHMQPILDSIIRLLTSPLIQGLALNSLLNLFTAIVKTDFPEKPTFESLLDSVTSPVYDNVALSRHAHMAIASCAAVITESTQNLEKSRSLAKKLAQQLQTANMSDSIRLFAMITLGELGRRVPDTYSPDFPVKPEDLAIKAFNHHHEDLKSAAAQALGALAVGNLNVYLPFILEQIRTQPKKQYLLLHALKEVIVWESSSEESTKSTDLFRSAIVDIWGMLMANAGGNEDGTRSVVAECLGRLCSFDPESLLPKLKESMRSSDPAIRSSAVSAIKYMINDEKRIVDITLQKQIGDFLAAVRDEDLKVRRVALVVLNSAAHNKPALVRDLLPDLLPAVYEETKLRKELIKEVEMGPFKHQVDEGLDLRKCAFECMFTLLESCVDKIDITQFSSVMEVGLSDQNHDVKLLNYLTLQRVANLAPGQVLQRIDRVCEPLKTQLNVRPRGNAVKQEVEKLEELKKAVIRVVYGLKLKLPEVERNPQFLDLYNTIKHTKELEALANDVLKESQRAVVYDTPMETA 4D0D62BAD14B83D2 cul2_caeel Cullin-2 cul-2 ccd-3 div-3 ZK520.4 protein protein caeel Caenorhabditis elegans MASSTKVSLRRPPRPQVMYSLKPKVVEFDKVWVQLRPSIIDIINLRPITNVQWHHKFSDVYDICVSIPTPLSERLYNEVKACIQEHVRQKRQDIVDVDPDLLLQEYHKMWRVFHEGAIFIHRLFGYLNKQFVKQKRCTDLDNFAQYAAFLQIPDVKEIGCLALEIWKEDLVKTILPQLVKLLLIAIDNDRKGNFPHIANEVSGVINSFVKMEETDFDVVPAEGARYKARESTTAFYQESFEKPLLTDTEQYYSALAQKMLTDLSCSEYMEQVIVLLEQEEMRAKKYLHESSVEKVITLCQKVMIKAHKDKLHAVCHDLITNEENKDLRNMYRLLKPIQAGLSVMVKEFEEYVKKKGLEAVSRLTGENVPQQFVENVLRVYNKFNDMKTAVFMDDGEFSSGLDKALQGVVNSKEPGQSVPKASERLARYTDGLLKKSTKGLSETDLEAKLDSAIVIFRYIEDKDIFQKFYSKMLANRLIASTSISMDAEELMINKLKQACGYEFTSKLSRMFTDIGLSQELSNNFDKHIADIKTVQPDVKFVPTQTMILQAGSWPLNAPQLSTNSNNQTAQDVANFHLPRILQPVIQEFEKFYTGKHNGRKLTWLFNMSQGDVRLTYLDKQYVAQMYVYQMAALLCFERRDAILVKDIGEEIGVSGDYLLKTIRTILDVTLLTCDDQNLTADSLVRLNMSMTSKRMKFRLQAPQVNKAVEKEQEAVANTVSQDRKYYMECAIVRIMKTRKVLKHNALVTEIMDQTKGRFSPDVPFIKKSIEDLIEKMYIQRTDQNDEYQYLA 8993DF21905F0738 p34708-1 Sex-determining transformer protein 1 Tra-1L tra-1 her-2 Hermaphrodization of XO animals protein 2 Y47D3A.6 protein protein caeel Caenorhabditis elegans MMAPSTEDPDTVVEAQRRGSFSKKKNANGWNKVELVDQCAKQMGSEDKQPGGGDVKTENDPSKNGLGSATSNFIQSSVPPSHQTLSNPLQLSPPAEASVAQQSGASQVFPTFQAALGASSDELLQPNATSSSTSSSASTSSIVPVVKFTNQTAPNGSTVATSVGQNVRLTINGKRVGRPPGTFKRPQNNAANSSNSGNDSDMMGDHDLTCRWKSCNSSFQTLKALVDHVQESHVQSTEQEHHAWRCEWEGCDRNETFKALYMLIVHVRRHTGEKPNKCEYPGCGKEYSRLENLKTHRRTHTGEKPYKCEFADCEKAFSNASDRAKHQNRTHSNLKPYSCQIPQCTKSYTDPSSLRKHIKAVHGDDEYEKAKKSRPANYSNRRRPDHRLAPPTGAMSHPYLATPNSGASVVAHSSVHQQNFINMALAQHHHNAQRAQQLMAATGNVMPMMDPASAAAAAQAQAHHQAQAQMLQTHMMQQAQIQAAAQMQAQVQHQAAMQAHAMQQAQMVLQNNLLGAQSLLSPFSPLLPPSRAPNVMAMLQTPPTPTSVAPMFDIMTSRAPMAPVVSAPTAPAPLVPAPVPASPVFDELREQMREVEPLQQQQQQEPMDQDLQDIRVDGDSDDEDEEEPRTPSGALLLPRGGNNGDGGFGGSGSSRASSGSGTMELSAAPISQNGSRASGSGERGMRSFLIADILQLAADFQNERLLSDVLDLAIFDTRDVRSLHNIYQVYIRAHKAIPITRRPLDWNETHQLHNLYHDPRFNRAEHQDSPAIRDRDTRFWRTIAEANTMRQRQIEPVPLDDDDEGYFDEMVHRVQNGRLNEQFMEGFESDDDDGFEDEDDVPGLGIAVYRGRRRVRREALKQANLDIQEAETAGRNVGGFGDEEDRNNRGHDQDRSFLDHYYPPMVVVVETESPQIVRDQEMMRQFEEAKKNVETDEIKKRAEAMQFGTSSSHHHTKTLLIQRALFDKTSSVRRSLLQFITISVDQEELRQSCHATSAPQGAHVVHNVVDEFDSIMRAQEDSNNRILLSLDIPAPSAVTGVSGSITHADNSALQLQQEQPTSSFSSWFPEDDPIYALPPPPPPPAPPRRRRSADNKDDSENIPKKPRHQF EEE72C82841ECD7A cul2-elc1-1 4 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey physical association physical association Fig 1A fem1-elc1-1 3 14 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region binding region binding-associated region binding region mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 1C elc1-fem1-1 2 14 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 mutation analysis mutation analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 12 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) physical association physical association Fig 1D fem1-tra1a-1 3 14 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 17 unspecified role unspecified role prey prey n-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 5A, 6A Interaction is observed only in the presence of the proteasome inhibitor N-acetyl-L-leucinyl- L-leucinal-L-norleucinal (LLnL). fem1-cul2-1 3 14 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role prey prey physical association physical association Fig 5B cul2-tra1a-1 1 16 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 17 unspecified role unspecified role prey prey physical association physical association Fig 5C Interaction is observed only when pbs-4 RNAi is used to inactivate the proteasome. fem2-tra1a-1 3 6 unspecified role unspecified role bait bait n-terminus vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 17 unspecified role unspecified role prey prey n-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 6A Interaction is observed only in the presence of the proteasome inhibitor N-acetyl-L-leucinyl- L-leucinal-L-norleucinal (LLnL). fem3-tra1a-1 3 17 unspecified role unspecified role prey prey n-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role bait bait n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 6A Interaction is observed only in the presence of the proteasome inhibitor N-acetyl-L-leucinyl- L-leucinal-L-norleucinal (LLnL). fem2-fem1-1 2 6 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 14 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 mutation analysis mutation analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 6B fem2-tra1a-2 2 6 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 17 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 6B fem3-tra1a-2 2 10 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 17 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 6B fem3-fem1-1 2 14 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 10 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) physical association physical association Fig 6B tra1a-fem1-1 2 14 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 17 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) physical association physical association Fig 6C fem1-fem2-1 3 6 unspecified role unspecified role prey prey n-terminus vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 14 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role prey prey n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 6D fem2-fem1-2 3 6 unspecified role unspecified role bait bait n-terminus vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 14 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role prey prey n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 6D fem3-fem1-2 3 6 unspecified role unspecified role prey prey n-terminus vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 14 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role bait bait n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 6D elc1-fem1-2 3 6 unspecified role unspecified role prey prey n-terminus vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 14 unspecified role unspecified role prey prey n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey n-terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role bait bait n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig 6E, 7B