UniProt UniProt http://www.uniprot.org MEK1 and protein phosphatase 4 coordinate Dictyostelium development and chemotaxis. MEK1 and protein phosphatase 4 coordinate Dictyostelium development and chemotaxis. Mol. Cell. Biol. (0270-7306) 2007 imex curation Mendoza MC., Booth EO., Shaulsky G., Firtel RA. phosphatase assays with t7-pp4r2 precipitates (Q86IL5) not annotated. Measured with background pp4c deletion diminishes phosphatase activity, indicating a pp4c-pp4r2 interaction (Table I). Substrate is the KRpTIRR peptide from the Ser/Thr assay kit (see m+m). rafirtel@ucsd.edu orchard Only protein-protein interactions dicdi Dictyostelium discoideum (Slime mold) Slime mold anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mendoza MC., Booth EO., Shaulsky G., Firtel RA. phosphatase assays with t7-pp4r2 precipitates (Q86IL5) not annotated. Measured with background pp4c deletion diminishes phosphatase activity, indicating a pp4c-pp4r2 interaction (Table I). Substrate is the KRpTIRR peptide from the Ser/Thr assay kit (see m+m). rafirtel@ucsd.edu Accepted 2008-DEC-10 by ORCHARD. Mol. Cell. Biol. (0270-7306) 2007 orchard Only protein-protein interactions imex curation smek_dicdi Suppressor of Mek1 smkA DDB_G0289067 protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MEPLRKRVKVYQLDNSGKWDDKGTGHVSCIYVDALCAMGLIVRSESDNSVILQTRLSAEDIYQKQQDSLIVWTEPDSQLDLALSFQDSLGCQDIWENILQYQNQRTGSCDSVDLDLPPVSINNLQTINELLEASLPMLDKDKIINSIFKEDLVRSLLDLFDEIEKSGEGGVHLFQIFNIFKNLILFNDTSILEVILSEDYLVRVMGALEYDPEISENNRIKHREFLNQQVVFKQVIKFPSKSLIGTIHQTFRIQYLKDVVLPRVLDDVTFSSLNSLIYFNNIDIVSQIQNDSDFLENLFSEIQKSEKNSEERKDLILFLQDLCNLAKGLQIQSKSTFFTVVVSLGLFKTLSAILDDENVQTRVSCTEIVLSTLLHDPEILRSYLCSPTSGNSKFLVQLINLFITDKDIGVKNQIVEIIKTLLEADSYDSSDFFRLFYDKGIDLLVSPLNEVYKGEPTIPGDPSSNLDSFVLYNIMELVIYCIKHHCYRIKHFIVEEGIAKKILRYTNPTGSGGGGGGGGNSERYLILGSIRFFRSMVNMKDDLYNQHIIQENLFEPIIEVFKSNISRYNLLNSAIIELFQYIYKENIRDLIVYLVERYRELFESVTYTDVLKQLILKYEQIKDSSFESPETSCNNNDSSSNDIDSKPIIGNNKINHNYQRTQREIDEEEEEAYFNRDDDSEDSDDEDELIPISINNNNNNNNNNKQICTNNENNMEKNDDNIEKDNENTNNGNGSSHIKIVDYEDEDDEDDEINKSVESDDIVEKHEIIDKNEKKDEIMKENNDSDNDDNDNNDNDNDNDNNSDIENKNHLNNNGNNENNENNDDVQDKSNNKNNSDKINEDEKIEKQDEMKENLEMEEIDEKVKEKQPKDIKKENQSQPDETVFNGKSNNSNNNNNNNNNNSNNQEIGDNRKTTPKRKLDYEKNESVVSKKIDKSNGPTSIDKDINGCDESPNKKLNNNNSNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNQNDENELSSASEEEEEQLENGKHIKKFKRGKKDSNNSSNNSNNSSPTPSELHV BE7680EFA773B572 pp4c_dicdi Serine/threonine-protein phosphatase 4 catalytic subunit ppp4c pppC DDB_G0272116 protein protein dicdi Dictyostelium discoideum (Slime mold) Slime mold MSSDLDRQIEQLKRCEIIKESEVRALCSKAREILLEEGNVQRVDSPVTICGDIHGQFYDLKELFKVGGDCPQTNYLFMGDFVDRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQITQVYGFYEECVRKYGSVTVWKYCTEIFDYLSLSALVDGKIFCVHGGLSPSINTLDQIRAIDRKQEVPHEGPMCDLMWSDPEDIPGWNGSPRGAGFLFGEDVVQKFNHDNNLEFICRAHQLVMEGFKYMFNETLVTVWSAPNYCYRCGNVAAILQLDENLKKNFAIFEAAPQESRGAPAKKPAPEYFL C0903104ADA93D41 ppp4c-smka 1 2 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association The interaction was observed in vegetative and developmental cells (treated with cAMP pulsing). 1A ppp4c-smka-1 1 2 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association The interaction was observed in vegetative and developmental cells (treated with cAMP pulsing). 1B HA-SMEKdeltaEVH1 and HA-EVH1 constructs used to determine the binding site are according PMID:16107728 (same labaratory).