IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Arabidopsis WEE1 kinase controls cell cycle arrest in response to activation of the DNA integrity checkpoint. Arabidopsis WEE1 kinase controls cell cycle arrest in response to activation of the DNA integrity checkpoint. Plant Cell (1040-4651) 2007 imex curation De Schutter K., Joubes J., Cools T., Verkest A., Corellou F., Babiychuk E., Van Der Schueren E., Beeckman T., Kushnir S., Inze D., De Veylder L. lieven.deveylder@psb.ugent.be 03-Feb-2009: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined De Schutter K., Joubes J., Cools T., Verkest A., Corellou F., Babiychuk E., Van Der Schueren E., Beeckman T., Kushnir S., Inze D., De Veylder L. lieven.deveylder@psb.ugent.be Accepted 2008-Not a month!-12 by JYOTI. 03-Mar-2009: Contacted by JYOTI. Plant Cell (1040-4651) 2007 jyoti Only protein-protein interactions imex curation wee1_arath Wee1-like protein kinase WEE1 Wee1-At F22D16.3 At1g02970 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MFEKNGRTLLAKRKTQGTIKTRASKKIRKMEGTLERHSLLQFGQLSKISFENRPSSNVASSAFQGLLDSDSSELRNQLGSADSDANCGEKDFILSQDFFCTPDYITPDNQNLMSGLDISKDHSPCPRSPVKLNTVKSKRCRQESFTGNHSNSTWSSKHRVDEQENDDIDTDEVMGDKLQANQTERTGYVSQAAVALRCRAMPPPCLKNPYVLNQSETATDPFGHQRSKCASFLPVSTSGDGLSRYLTDFHEIRQIGAGHFSRVFKVLKRMDGCLYAVKHSTRKLYLDSERRKAMMEVQALAALGFHENIVGYYSSWFENEQLYIQLELCDHSLSALPKKSSLKVSEREILVIMHQIAKALHFVHEKGIAHLDVKPDNIYIKNGVCKLGDFGCATRLDKSLPVEEGDARYMPQEILNEDYEHLDKVDIFSLGVTVYELIKGSPLTESRNQSLNIKEGKLPLLPGHSLQLQQLLKTMMDRDPKRRPSARELLDHPMFDRIRG 7E7C32697B9A9E44 cdka1_arath Cyclin-dependent kinase A-1 CDKA-1 Cell division control protein 2 homolog A CDC2 At3g48750 CDC2A T21J18.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDQYEKVEKIGEGTYGVVYKARDKVTNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHSNIVKLQDVVHSEKRLYLVFEYLDLDLKKHMDSTPDFSKDLHMIKTYLYQILRGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGSHHYSTPVDIWSVGCIFAEMISQKPLFPGDSEIDQLFKIFRIMGTPYEDTWRGVTSLPDYKSAFPKWKPTDLETFVPNLDPDGVDLLSKMLLMDPTKRINARAALEHEYFKDLGGMP B5FAE55FA9EC366E cdka1-wee1 1 2 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association Figure 1B To analyze whether the Arabidopsis WEE1 kinase might be responsible for the observed Tyr phosphorylation of CDKA;1, both proteins were tested for their interaction using the yeast two-hybrid system. CDKA;1 and CDKB1;1 in fusion with the GAL4 DNA binding domain were cotransformed in an appropriate yeast reporter strain with an empty control vector or a vector encoding a fusion protein between the GAL4 transactivation domain and WEE1. Transformants were streaked on medium with or without His. Cells expressing CDKA;1 and WEE1 grew in the absence of His, indicating that both gene products interacted. No association was observed between CDKB1;1 and WEE1