IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Characterization of a two-component high-affinity nitrate uptake system in Arabidopsis. Physiology and protein-protein interaction. Characterization of a two-component high-affinity nitrate uptake system in Arabidopsis. Physiology and protein-protein interaction. Plant Physiol. (0032-0889) 2006 imex curation Orsel M., Chopin F., Leleu O., Smith SJ., Krapp A., Daniel-Vedele F., Miller AJ. The corresponding author confirmed the accession numbers used for the proteins identified in this study. tony.miller@bbsrc.ac.uk 16-April-2008: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 ub reconstruction ubiquitin reconstruction ub reconstruction predetermined predetermined participant predetermined Orsel M., Chopin F., Leleu O., Smith SJ., Krapp A., Daniel-Vedele F., Miller AJ. The corresponding author confirmed the accession numbers used for the proteins identified in this study. tony.miller@bbsrc.ac.uk Accepted 2008-APR-18 by JYOTI. Plant Physiol. (0032-0889) 2006 Only protein-protein interactions imex curation nrt31_arath High-affinity nitrate transporter 3.1 NRT3.1 At5g50200 NAR2.1 NAR2.2 WR3 Protein WOUND-RESPONSIVE 3 K6A12.6 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLNITWTLSSIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPYDKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAKIEQSK 5E82A99EE721508E nrt23_arath High affinity nitrate transporter 2.3 At5g60780 NRT2.3 MAE1.3 MAE1.4 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTHNHSNEEGSIGTSLHGVTAREQVFSFSVDASSQTVQSDDPTAKFALPVDSEHRAKVFNPLSFAKPHMRAFHLGWLSFFTCFISTFAAAPLVPIIRDNLDLTKTDIGNAGVASVSGAIFSRLAMGAVCDLLGARYGTAFSLMLTAPTVFSMSFVGGPSGYLGVRFMIGFCLATFVSCQYWTSVMFNGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLAGATPFMAWRIAFFVPGFLQVVMGILVLSLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFVLYGSSMGIELTINNVISGYFYDRFNLKLQTAGIVAASFGMANFIARPFGGYASDVAARVFGMRGRLWTLWIFQTVGALFCIWLGRASSLPIAILAMMLFSIGTQAACGALFGVAPFVSRRSLGLISGLTGAGGNFGSGLTQLLFFSSARFSTAEGLSLMGVMAVLCTLPVAFIHFPQWGSMFLRPSTDGERSQEEYYYGSEWTENEKQQGLHEGSIKFAENSRSERGRKVALANIPTPENGTPSHV 83008E06DD595051 nrt21_arath High-affinity nitrate transporter 2.1 NRT2.1 ACH1 At1g08090 LIN1 NRT2;1AT Protein ACH1 Protein LATERAL ROOT INITIATION 1 T6D22.17 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKPHMRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGAVCDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGILVLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQTAGGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGNFGSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTEAHYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV A40F435039635090 nrt21_chlre Nitrate transporter 2.1 NRT2.1 NAR-3 NAR3 Nitrate assimilation related protein 3 CHLREDRAFT_192090 protein protein chlre Chlamydomonas reinhardtii Chlamydomonas smithii MAEKPATVNAELVKEMDAAPKKYPYSLDSEGKANYCPVWRFTQPHMMAFHLSWICFFMSFVATFAPASLAPIIRDDLFLTKSQLGNAGVAAVCGAIAARIFMGIVVDSIGPRYGAAATMLMTAPAVFCMALVTDFSTFACVRFFIGLSLCMFVCCQFWCGTMFNVKIVGTANAIAAGWGNMGGGACHFIMPLIYQGIKDGGVPGYQAWRWAFFVPGGIYILTATLTLLLGIDHPSGKDYRDLKKEGTLKAKGAMWPVVKCGLGNYRSWILALTYGYSFGVELTVDNVIVEYLFDQFGLNLAVAGALGAIFGLMNLFTRATGGMISDLVAKPFGMRGRIWALWIIQTLGGIFCIVLGKVSNSLSSTIVIMIVFSIFCQQACGLHFGITPFVSRRAYGVVSGLVGAGGNTGAAITQAIWFAGTAPWQLTLSKADGFVYMGIMTIGLTLPLFFIWFPMWGSMLTGPREGAEEEDYYMREWSAEEVASGLHQGSMRFAMESKSQRGTRDKRAAGPARVPQQLRLGARCCQARGGLIRCVLAQSLGATGCSD DCDBF86126EE0B2B nar2_1-ach1 1 4 unspecified role unspecified role prey prey region N-terminal ubiquitin tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 2 unspecified role unspecified role bait bait region C-terminal ubiquitin tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 1 Growth of diploid cells under selective conditions revealed interaction of AtNAR2.1-CubPLV with NubG-AtNRT2.1 and NubG-AtNRT2.3, but not with NubG-CrNRT2.1 The authors state "The activity detected when AtNAR2.1-CubPLV was in combination with NubG-AtRNT2.1was 2 and 16 times higher than in combination with NubG-AtNRT2.3 and NubG-CrNRT2.1, respectively". nar2_1-at5g60780 1 2 unspecified role unspecified role bait bait region C-terminal ubiquitin tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey region N-terminal ubiquitin tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 1 Growth of diploid cells under selective conditions revealed interaction of AtNAR2.1-CubPLV with NubG-AtNRT2.1 and NubG-AtNRT2.3, but not with NubG-CrNRT2.1 The authors state "The activity detected when AtNAR2.1-CubPLV was in combination with NubG-AtRNT2.1was 2 and 16 times higher than in combination with NubG-AtNRT2.3 and NubG-CrNRT2.1, respectively". nar2_1-nar3 1 2 unspecified role unspecified role bait bait region C-terminal ubiquitin tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region N-terminal ubiquitin tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 1B The activity detected when AtNAR2.1-CubPLV was in combination with NubG-AtRNT2.1was 2 and 16 times higher than in combination with NubG-AtNRT2.3 and NubG-CrNRT2.1, respectively. The authors state "The activity detected when AtNAR2.1-CubPLV was in combination with NubG-AtRNT2.1was 2 and 16 times higher than in combination with NubG-AtNRT2.3 and NubG-CrNRT2.1, respectively". This interaction was only detected by b-galactosidase activity assay not growth assya on selective media.