IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ APETALA1 and SEPALLATA3 interact with SEUSS to mediate transcription repression during flower development. APETALA1 and SEPALLATA3 interact with SEUSS to mediate transcription repression during flower development. Development (0950-1991) 2006 Sridhar VV., Surendrarao A., Liu Z. zliu@umd.edu 25-Jun-2008: Contacted by JYOTI. orchard in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Sridhar VV., Surendrarao A., Liu Z. zliu@umd.edu Accepted 2008-JUN-15 by ORCHARD. 25-Jun-2008: Contacted by JYOTI. Development (0950-1991) 2006 orchard APETALA1 and SEPALLATA3 interact with SEUSS to mediate transcription repression during flower development. APETALA1 and SEPALLATA3 interact with SEUSS to mediate transcription repression during flower development. Development (0950-1991) 2006 Sridhar VV., Surendrarao A., Liu Z. zliu@umd.edu 25-Jun-2008: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sridhar VV., Surendrarao A., Liu Z. zliu@umd.edu Accepted 2008-JUN-15 by ORCHARD. Development (0950-1991) 2006 orchard seuss_arath Transcriptional corepressor SEUSS SEU F28H19.10 At1g43850 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVPSEPPNPVGGGENVPPSILGGQGGAPLPSQPAFPSLVSPRTQFGNNMSMSMLGNAPNISSLLNNQSFVNGIPGSMISMDTSGAESDPMSNVGFSGLSSFNASSMVSPRSSGQVQGQQFSNVSANQLLAEQQRNKKMETQSFQHGQQQSMQQQFSTVRGGGLAGVGPVKMEPGQVSNDQQHGQVQQQQQKMLRNLGSVKLEPQQIQAMRNLAQVKMEPQHSEQSLFLQQQQRQQQQQQQQQFLQMPGQSPQAQMNIFQQQRLMQLQQQQLLKSMPQQRPQLPQQFQQQNLPLRPPLKPVYEPGMGAQRLTQYMYRQQHRPEDNNIEFWRKFVAEYFAPNAKKRWCVSMYGSGRQTTGVFPQDVWHCEICNRKPGRGFEATAEVLPRLFKIKYESGTLEELLYVDMPRESQNSSGQIVLEYAKATQESVFEHLRVVRDGQLRIVFSPDLKIFSWEFCARRHEELIPRRLLIPQVSQLGSAAQKYQQAAQNATTDSALPELQNNCNMFVASARQLAKALEVPLVNDLGYTKRYVRCLQISEVVNSMKDLIDYSRETRTGPIESLAKFPRRTGPSSALPGPSPQQASDQLRQQQQQQQQQQQQQQQQQQQQQQQQTVSQNTNSDQSSRQVALMQGNPSNGVNYAFNAASASTSTSSIAGLIHQNSMKGRHQNAAYNPPNSPYGGNSVQMQSPSSSGTMVPSSSQQQHNLPTFQSPTSSSNNNNPSQNGIPSVNHMGSTNSPAMQQAGEVDGNESSSVQKILNEILMNNQAHNNSSGGSMVGHGSFGNDGKGQANVNSSGVLLMNGQVNNNNNTNIGGAGGFGGGIGQSMAANGINNINGNNSLMNGRVGMMVRDPNGQQDLGNQLLGAVNGFNNFDWNA 636D83BAD3946467 sep3_arath Developmental protein SEPALLATA 3 Agamous-like MADS-box protein AGL9 SEP3 AGL9 At1g24260 F3I6.19 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIIFSNRGKLYEFCSSSSMLRTLERYQKCNYGAPEPNVPSREALAVELSSQQEYLKLKERYDALQRTQRNLLGEDLGPLSTKELESLERQLDSSLKQIRALRTQFMLDQLNDLQSKERMLTETNKTLRLRLADGYQMPLQLNPNQEEVDHYGRHHHQQQQHSQAFFQPLECEPILQIGYQGQQDGMGAGPSVNNYMLGWLPYDTNSI DIP protein O22456 original sequence version: 117 DIP protein O22456 original sequence version: 110 0057CABD3F1AFC40 leung_arath Transcriptional corepressor LEUNIG LUG Protein ROTUNDA2 RON2 L23H3.30 At4g32551 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSQTNWEADKMLDVYIHDYLVKRDLKATAQAFQAEGKVSSDPVAIDAPGGFLFEWWSVFWDIFIARTNEKHSEVAASYIETQMIKAREQQLQQSQHPQVSQQQQQQQQQQIQMQQLLLQRAQQQQQQQQQQHHHHQQQQQQQQQQQQQQQQQQQQHQNQPPSQQQQQQSTPQHQQQPTPQQQPQRRDGSHLANGSANGLVGNNSEPVMRQNPGSGSSLASKAYEERVKMPTQRESLDEAAMKRFGDNVGQLLDPSHASILKSAAASGQPAGQVLHSTSGGMSPQVQTRNQQLPGSAVDIKSEINPVLTPRTAVPEGSLIGIPGSNQGSNNLTLKGWPLTGFDQLRSGLLQQQKPFMQSQSFHQLNMLTPQHQQQLMLAQQNLNSQSVSEENRRLKMLLNNRSMTLGKDGLGSSVGDVLPNVGSSLQPGGSLLPRGDTDMLLKLKMALLQQQQQNQQQGGGNPPQPQPQPQPLNQLALTNPQPQSSNHSIHQQEKLGGGGSITMDGSISNSFRGNEQVLKNQSGRKRKQPVSSSGPANSSGTANTAGPSPSSAPSTPSTHTPGDVISMPNLPHSGGSSKSMMMFGTEGTGTLTSPSNQLADMDRFVEDGSLDDNVESFLSQEDGDQRDAVTRCMDVSKGFTFTEVNSVRASTTKVTCCHFSSDGKMLASAGHDKKAVLWYTDTMKPKTTLEEHTAMITDIRFSPSQLRLATSSFDKTVRVWDADNKGYSLRTFMGHSSMVTSLDFHPIKDDLICSCDNDNEIRYWSINNGSCTRVYKGGSTQIRFQPRVGKYLAASSANLVNVLDVETQAIRHSLQGHANPINSVCWDPSGDFLASVSEDMVKVWTLGTGSEGECVHELSCNGNKFQSCVFHPAYPSLLVIGCYQSLELWNMSENKTMTLPAHEGLITSLAVSTATGLVASASHDKLVKLWK 7CB879744496A8AA ap1_arath Floral homeotic protein APETALA 1 Agamous-like MADS-box protein AGL7 AP1 AGL7 At1g69120 F4N2.9 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGRGRVQLKRIENKINRQVTFSKRRAGLLKKAHEISVLCDAEVALVVFSHKGKLFEYSTDSCMEKILERYERYSYAERQLIAPESDVNTNWSMEYNRLKAKIELLERNQRHYLGEDLQAMSPKELQNLEQQLDTALKHIRTRKNQLMYESINELQKKEKAIQEQNSMLSKQIKEREKILRAQQEQWDQQNQGHNMPPPLPPQQHQIQHPYMLSHQPSPFLNMGGLYQEDDPMAMRRNDLELTLEPVYNCNLGCFAA DIP protein P35631 original sequence version: 125 01E26DE18CE3478C seu-ap1 2 6 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range certain certain sequence position certain 3 unspecified role unspecified role bait bait physical association physical association Figure 1A Figure 1B SEU interacted strongly with AP1 The authors do not provide coordinates for the C-terminal region which interacts with SEUSS. The reference provided by the authors Honma et al (2001) (PMID 11206550), provides the coordinates of the MIK domain containing constructs used in this study. The C-terminal domain was taken as the protein truncated at the MIK domain and fuzzy coordinates used to indicate that this is a 'best guess' by the curator. The length of the protein was obtained from the UniProt record. seu-sep3 2 3 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range certain certain sequence position certain physical association physical association Figure 1A Figure 1B Interactions between SEU and AP1/SEP3 in yeast and in vitro. The authors do not provide coordinates for the C-terminal region which interacts with SEUSS. The reference provided by the authors Honma et al (2001) (PMID 11206550), provides the coordinates of the MIK domain containing constructs used in this study. The C-terminal domain was taken as the protein truncated at the MIK domain and fuzzy coordinates used to indicate that this is a 'best guess' by the curator. The length of the protein was obtained from the UniProt record. seu-ap1-1 1 3 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 6 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind range range certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Figure 1C An in vitro pull-down assay showing 35S-labeled AP1 and SEP3 proteins retained by GST-SEU The authors do not provide coordinates for the C-terminal region which interacts with SEUSS. The reference provided by the authors Honma et al (2001) (PMID 11206550), provides the coordinates of the MIK domain containing constructs used in this study. The C-terminal domain was taken as the protein truncated at the MIK domain and fuzzy coordinates used to indicate that this is a 'best guess' by the curator. The length of the protein was obtained from the UniProt record. seu-sep3-1 1 3 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal required to bind necessary binding region required to bind range range certain certain sequence position certain in vitro In vitro direct interaction direct interaction Figure 1C An in vitro pull-down assay showing 35S-labeled AP1 and SEP3 proteins retained by GST-SEU The authors do not provide coordinates for the C-terminal region which interacts with SEUSS. The reference provided by the authors Honma et al (2001) (PMID 11206550), provides the coordinates of the MIK domain containing constructs used in this study. The C-terminal domain was taken as the protein truncated at the MIK domain and fuzzy coordinates used to indicate that this is a 'best guess' by the curator. The length of the protein was obtained from the UniProt record. lug-seu-ap1 1 6 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 3 unspecified role unspecified role ancillary ancillary region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Figure 1D SEU bridges an interaction between LUG and AP1/SEP3 lug-seu-sep3 1 3 unspecified role unspecified role ancillary ancillary region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Figure 1D SEU bridges an interaction between LUG and AP1/SEP3