IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The A-type cyclin CYCA2;3 is a key regulator of ploidy levels in Arabidopsis endoreduplication. The A-type cyclin CYCA2;3 is a key regulator of ploidy levels in Arabidopsis endoreduplication. Plant Cell (1040-4651) 2006 imex curation Imai KK., Ohashi Y., Tsuge T., Yoshizumi T., Matsui M., Oka A., Aoyama T. Transgenic Arabidopsis (ecotype Columbia) plants which expressed the GFP tagged CYCA2:3 protein were used in this study. aoyama@sci-kyoto-u.ac.jp 16-Oct-2008: Contacted by JYOTI. orchard Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Imai KK., Ohashi Y., Tsuge T., Yoshizumi T., Matsui M., Oka A., Aoyama T. Transgenic Arabidopsis (ecotype Columbia) plants which expressed the GFP tagged CYCA2:3 protein were used in this study. aoyama@sci-kyoto-u.ac.jp Accepted 2008-SEP-26 by ORCHARD. 16-Oct-2008: Contacted by JYOTI. Plant Cell (1040-4651) 2006 orchard Only protein-protein interactions imex curation cdka1_arath Cyclin-dependent kinase A-1 CDKA-1 Cell division control protein 2 homolog A CDC2 At3g48750 CDC2A T21J18.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDQYEKVEKIGEGTYGVVYKARDKVTNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHSNIVKLQDVVHSEKRLYLVFEYLDLDLKKHMDSTPDFSKDLHMIKTYLYQILRGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGSHHYSTPVDIWSVGCIFAEMISQKPLFPGDSEIDQLFKIFRIMGTPYEDTWRGVTSLPDYKSAFPKWKPTDLETFVPNLDPDGVDLLSKMLLMDPTKRINARAALEHEYFKDLGGMP B5FAE55FA9EC366E cca23_arath Cyclin-A2-3 CYCA2-3 CYC3C T16N11.8 At1g15570 G2/mitotic-specific cyclin-A2-3 Cyclin-3c Cyc3c-At protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGKENAVSRPFTRSLASALRASEVTSTTQNQQRVNTKRPALEDTRATGPNKRKKRAVLGEITNVNSNTAILEAKNSKQIKKGRGHGLASTSQLATSVTSEVTDLQSRTDAKVEVASNTAGNLSVSKGTDNTADNCIEIWNSRLPPRPLGRSASTAEKSAVIGSSTVPDIPKFVDIDSDDKDPLLCCLYAPEIHYNLRVSELKRRPLPDFMERIQKDVTQSMRGILVDWLVEVSEEYTLASDTLYLTVYLIDWFLHGNYVQRQQLQLLGITCMLIASKYEEISAPRIEEFCFITDNTYTRDQVLEMENQVLKHFSFQIYTPTPKTFLRRFLRAAQASRLSPSLEVEFLASYLTELTLIDYHFLKFLPSVVAASAVFLAKWTMDQSNHPWNPTLEHYTTYKASDLKASVHALQDLQLNTKGCPLSAIRMKYRQEKYKSVAVLTSPKLLDTLF 8EF3B638843CD478 cyca2_3-cdka_1 1 3 unspecified role unspecified role bait bait c-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 2 unspecified role unspecified role prey prey physical association physical association Figure 8A The immunoprecipitates were examined for the presence of CDKA;1 by protein gel blot analysis with an antibody specific to the peptide sequence PSTAIR, which CDKA;1 only contains in Arabidopsis. A signal band was detected at a position corresponding to the 34-kD band (Figure 8A), indicating that mDB-CYCA2;3-GFP binds to CDKA;1 in planta