IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ In vitro recombination cloning of entire cDNA libraries in Arabidopsis thaliana and its application to the yeast two-hybrid system. In vitro recombination cloning of entire cDNA libraries in Arabidopsis thaliana and its application to the yeast two-hybrid system. Funct. Integr. Genomics (1438-793X) 2005 Burkle L., Meyer S., Dortay H., Lehrach H., Heyl A. The text in the paper states that "AHP3 clones also interacted when ARR2 was used as bait". However, Table 5 presents the results of the yeast two hybrid screens and shows ARR2 interacts with AHP1. Therefore this ambiguous interaction was not curated. Arabidopsis (ecotype Columbia) cDNA library from seedlings treated with various plant hormones at 1 micro mole concentration (abscisic acid,1-aminocyclopropane-1-carboxylic acid, benzyladenine, gibberellic acid or alpha-naphthalene acid). orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Burkle L., Meyer S., Dortay H., Lehrach H., Heyl A. The text in the paper states that "AHP3 clones also interacted when ARR2 was used as bait". However, Table 5 presents the results of the yeast two hybrid screens and shows ARR2 interacts with AHP1. Therefore this ambiguous interaction was not curated. Accepted 2008-JUL-2 by ORCHARD. Arabidopsis (ecotype Columbia) cDNA library from seedlings treated with various plant hormones at 1 micro mole concentration (abscisic acid,1-aminocyclopropane-1-carboxylic acid, benzyladenine, gibberellic acid or alpha-naphthalene acid). Funct. Integr. Genomics (1438-793X) 2005 orchard ahp3_arath Histidine-containing phosphotransfer protein 3 AHP3 ATHP2 At5g39340 MUL8.2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDTLIAQLQRRFCDFTISLYHQGFLDDQFTELKKLQDECSPDFVAEVVTLFFEDCEKLISNMARALDQTGNVDFKLVGSSVHQLKGSSSSVGAKRVKGLCVTLKECCDSQNYEGCVRCLQQVDIEYKTLKAKLQDLFNLEQQIVQAGGRIPQVDI F3B26DCFA3904E0D frl4a_arath FRIGIDA-like protein 4a FRL4A MCB17.20 At3g22440 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGSVPDPGELTELAQPSFEEFQKQTSLMTSCTLLWQELSDHFTSLEQNLMKKSEALKQMIETLDNQTQTSLESLKRREVTIDHSVEIVAGKVGERARAALESLEKARDGCGDGSNDDSGDVDDEEGLLSALKSLCLKMDARGFWNFVTARKKELENLRSKIPAALVDCVDPAMLVLEAISEVFPVDTRGDKVSNDYGWACVVILESLTPVIVDPVIGKSRLLVTPSVKEKAKEIAETWKKSLEERGRIENVKTPDVHTFLQHLVTFGIVKSEDLALYRKLVVGSAWRKQMPKLAVSVGLGDQMPDMIEELISRGQQLDAVHFTYEVGLVDKFPPVPLLKAYLRDAKKSAASIMEDSSNTGRATHLVARKEQSALKAVLKCIEEYKLEEEFPPENLKKRLDQLEKTKTEKRKPAAVPANKRTRASYNGPMPPAKAGRITNAYVSSFPFIRSPSHSPQYASPAAYPSPPTTVYSNRSPPYPYSPEIIPGSYQGSPIGYPAYNGYCNGPVPAPAPPVYHPHHHQHHQFHHQQHYY 8E888B066A339B04 a8mse0_arath At4g21105 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLTETPFRPREKLLEKQRLFQSIQRHTYLKGPMDKITSLFIPSCQIIA BD03922BE4B6C021 trpc_arath Indole-3-glycerol phosphate synthase, chloroplastic IGPS T1O3.19 At2g04400 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEGLVPVQRLPIKVASPSLYRCNNSVSIRRSISGFAMDRKINFRAPSQFSIRAQQSDLKESLAVSSSSVEDKGNVLRIKEWEVEMYQEELAISQGIRIRRKPPSKAPLGYSGPFELRLHNNDADSPRNILEEITWYKDVEVSRMKELNPLDVLKKAVEDAPPTRDFVGALRMAHKRTGFPGLIAEVKKASPSRGILKENFDPVEIAQAYEKGGAACLSVLTDQKYFQGGFENLEAIRSAGVKCPLLCKEFVVDPWQIYYARTKGADAVLLIAAVLADLEITFLLKICKKLSLAALVEVHDEREMGRVLGIEGIELVGINNRSLETFEVDISNTKKLLEGEHGRQIRERDMIVVGESGLFTPDDIAYVQAAGVKAVLVGESIVKQNDPEKGIAGLFGRNISHT 288B4925BAD9E0DC apt1_arath Adenine phosphoribosyltransferase 1, chloroplastic APT1 APT F17L21.24 At1g27450 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQTIIISPLVSHRLCLARAVPCNRLLNNHHRAPPSIRLSNHRSTTSLRLFSSAAASRDSEMATEDVQDPRIAKIASSIRVIPDFPKPGIMFQDITTLLLDTEAFKDTIALFVDRYKDKGISVVAGVEARGFIFGPPIALAIGAKFVPMRKPKKLPGKVISEEYSLEYGTDTIEMHVGAVEPGERAIIIDDLIATGGTLAAAIRLLERVGVKIVECACVIELPELKGKEKLGETSLFVLVKSAA 92ABF089CA66C0FD arr3_arath Two-component response regulator ARR3 ARR3 F23H11.25 At1g59940 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAKDGGVSCLRRSEMIGIGIGELESPPLDSDQVHVLAVDDSLVDRIVIERLLRITSCKVTAVDSGWRALEFLGLDDDKAAVEFDRLKVDLIITDYCMPGMTGYELLKKIKESTSFKEVPVVIMSSENVMTRIDRCLEEGAEDFLLKPVKLADVKRLRSYLTRDVKVAAEGNKRKLTTPPPPPPLSATSSMESSDSTVESPLSMVDDEDSLTMSPESATSLVDSPMRSPGLA CD4950AE87FAFC91 q9lxb9_arath F17I14.270 F17I14_270 At5g09540 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVSDGGGENNRAEADQLLATSEKLLASSDFHGAKTFAIRACEADPSRTDAADYILAIADTLLAGETTIGDSKVPDWYAVLRISRLTQSPEHVATQYRRLTLLLKLNINRLPFADQALKLVSDAWYVLSDPPRKSIYDRELQLSQTGQSEKFQDSPLQSQAETLENPTATSFWTACPYCFSLFEYPKGYEECTLRCQQCRKAFEAVKTQTPPVESNGEGVYFGSWAMFPVGLTSHPNTSNWSPISHLSVCTGQSSSDQQSNLKATPRNDDVDIYIAISDDD 23D848ECC53FD1BC arr9_arath Two-component response regulator ARR9 ARR9 ATRR4 At3g57040 F24I3.120 Response reactor 4 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGMAAESQFHVLAVDDSLFDRKLIERLLQKSSCQVTTVDSGSKALEFLGLRQSTDSNDPNAFSKAPVNHQVVEVNLIITDYCMPGMTGYDLLKKVKESSAFRDIPVVIMSSENVPARISRCLEEGAEEFFLKPVRLADLNKLKPHMMKTKLKNQKLEEIETTSKVENGVPTAVADPEIKDSTNIEIEILPLQQDLLLVQQEEQTLSINNKRKSVEEGISTDRARPRFDGIATAV 489D89364F8585FC ahp1_arath Histidine-containing phosphotransfer protein 1 AHP1 ATHP3 At3g21510 MIL23.8 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDLVQKQKSLQDYTKSLFLEGILDSQFLQLQQLQDESNPDFVSQVVTLFFQDSDRILNDLSLSLDQQVVDFKKVDPHVHQLKGSSSSIGAQRVKNACVVFRSFCEQQNVEACHRCLQQVKQEYYLVKNRLETLFKLEQQIVASGGMIPAVELGF BA0C026EA19AF83D arr1_arath Two-component response regulator ARR1 ARR1 At3g16857 K20I9.9 MUH15.1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMNPSHGRGLGSAGGSSSGRNQGGGGETVVEMFPSGLRVLVVDDDPTCLMILERMLRTCLYEVTKCNRAEMALSLLRKNKHGFDIVISDVHMPDMDGFKLLEHVGLEMDLPVIMMSADDSKSVVLKGVTHGAVDYLIKPVRMEALKNIWQHVVRKRRSEWSVPEHSGSIEETGERQQQQHRGGGGGAAVSGGEDAVDDNSSSVNEGNNWRSSSRKRKDEEGEEQGDDKDEDASNLKKPRVVWSVELHQQFVAAVNQLGVEKAVPKKILELMNVPGLTRENVASHLQKYRIYLRRLGGVSQHQGNLNNSFMTGQDASFGPLSTLNGFDLQALAVTGQLPAQSLAQLQAAGLGRPAMVSKSGLPVSSIVDERSIFSFDNTKTRFGEGLGHHGQQPQQQPQMNLLHGVPTGLQQQLPMGNRMSIQQQIAAVRAGNSVQNNGMLMPLAGQQSLPRGPPPMLTSSQSSIRQPMLSNRISERSGFSGRNNIPESSRVLPTSYTNLTTQHSSSSMPYNNFQPELPVNSFPLASAPGISVPVRKATSYQEEVNSSEAGFTTPSYDMFTTRQNDWDLRNIGIAFDSHQDSESAAFSASEAYSSSSMSRHNTTVAATEHGRNHQQPPSGMVQHHQVYADGNGGSVRVKSERVATDTATMAFHEQYSNQEDLMSALLKQEGIAPVDGEFDFDAYSIDNIPV 8AB6D05DEA94EC4F arr1-ahp1-1 1 11 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Table 5 Using the prey library derived from the hormone-treated seedling, we were able to detect interactions with several different clones of AHPs, namely AHP1 and AHP3, when ARR1 was used as bait. arr1-ahp3-1 1 11 unspecified role unspecified role bait bait 2 unspecified role unspecified role prey prey physical association physical association Table 5 Using the prey library derived from the hormone-treated seedling, we were able to detect interactions with several different clones of AHPs, namely AHP1 and AHP3, when ARR1 was used as bait. arr3-at5g09540 1 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Table 5 ARR3 was found to interact with a DNA J-like protein (At5G21510). arr9-at4g21105 1 4 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Table 5 The function of At5G09540, the interaction partner of ARR9, is unknown. at2g04400-at3g22440 1 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Table 5 IGPS interacted with a member of the hydroxyproline-rich glycoprotein family apt1-apt1 1 6 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Table 5 Of the proteins with unknown interacting partners APRT was found to form homodimers,