IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ NapGH components of the periplasmic nitrate reductase of Escherichia coli K-12: location, topology and physiological roles in quinol oxidation and redox balancing. NapGH components of the periplasmic nitrate reductase of Escherichia coli K-12: location, topology and physiological roles in quinol oxidation and redox balancing. Biochem. J. (0264-6021) 2004 imex curation Brondijk TH., Nilavongse A., Filenko N., Richardson DJ., Cole JA. j.a.cole@bham.ac.uk 16-Dec-2008: Contacted by JYOTI. davet Only protein-protein interactions ecoli Escherichia coli (strain K12) adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Brondijk TH., Nilavongse A., Filenko N., Richardson DJ., Cole JA. j.a.cole@bham.ac.uk Accepted 2008-DEC-20 by DAVET. 16-Dec-2008: Contacted by JYOTI. Biochem. J. (0264-6021) 2004 davet Only protein-protein interactions imex curation napc_ecoli Cytochrome c-type protein NapC yejX napC b2202 JW2190 protein protein ecoli Escherichia coli (strain K12) MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGFNTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIRKLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTAQKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF E86A62ABE46AAAA3 naph_ecoli Ferredoxin-type protein NapH yejZ napH b2204 JW2192 Ubiquinol--[NapC cytochrome c] reductase NapH subunit protein protein ecoli Escherichia coli (strain K12) MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPWFGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKRLFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWINPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITVAATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFTITTRWSSGAKS DIP protein P33934 original sequence version: null 50AF7D5E0FFBC4D4 naph-napc 1 3 unspecified role unspecified role bait bait n terminal t25 tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 2 unspecified role unspecified role prey prey n terminus t18 tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Table 3 A strong interaction was shown between NapC and NapH, provided that the NapC tag was located on the N-terminus, which is consistent with the predicted localization of the C-terminus of NapC in the periplasm. napc-napc 1 2 unspecified role unspecified role bait bait n terminal t25 tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 2 unspecified role unspecified role prey prey n terminus t18 tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Table 3 There are strong indications that NapC, and possibly also NapH, forms a multimeric complex. naph-naph 1 3 unspecified role unspecified role prey prey n terminal t18 tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 3 unspecified role unspecified role bait bait n terminal t25 tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Table 3 any interaction between two tagged NapH proteins was fairly weak. naph-napc-1 1 3 unspecified role unspecified role prey prey n terminal t18 tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 2 unspecified role unspecified role bait bait n terminus t25 tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Table 3