IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Interaction between 52 kDa SSA/Ro and deubiquitinating enzyme UnpEL: a clue to function. Interaction between 52 kDa SSA/Ro and deubiquitinating enzyme UnpEL: a clue to function. Int. J. Biochem. Cell Biol. (1357-2725) 2001 Di Donato F., Chan EK., Askanase AD., Miranda-Carus M., Buyon JP. jill.buyon@med.nyu.edu 25-Jun-2008: Contacted by JYOTI. A fetal heart cDNA library, (constructed from the total RNA isolated from 18 fetal hearts), was used. davet yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Di Donato F., Chan EK., Askanase AD., Miranda-Carus M., Buyon JP. The positive control interaction of p53 and SV40 could not be curated as the source of p53 is not specified. jill.buyon@med.nyu.edu Accepted 2008-JUN-13 by DAVET. 25-Jun-2008: Contacted by JYOTI. A fetal heart cDNA library, (constructed from the total RNA isolated from 18 fetal hearts), was used. Int. J. Biochem. Cell Biol. (1357-2725) 2001 davet Interaction between 52 kDa SSA/Ro and deubiquitinating enzyme UnpEL: a clue to function. Interaction between 52 kDa SSA/Ro and deubiquitinating enzyme UnpEL: a clue to function. Int. J. Biochem. Cell Biol. (1357-2725) 2001 Di Donato F., Chan EK., Askanase AD., Miranda-Carus M., Buyon JP. jill.buyon@med.nyu.edu 25-Jun-2008: Contacted by JYOTI. A fetal heart cDNA library, (constructed from the total RNA isolated from 18 fetal hearts), was used. davet chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Di Donato F., Chan EK., Askanase AD., Miranda-Carus M., Buyon JP. The positive control interaction of p53 and SV40 could not be curated as the source of p53 is not specified. jill.buyon@med.nyu.edu Accepted 2008-JUN-13 by DAVET. 25-Jun-2008: Contacted by JYOTI. A fetal heart cDNA library, (constructed from the total RNA isolated from 18 fetal hearts), was used. Int. J. Biochem. Cell Biol. (1357-2725) 2001 davet Interaction between 52 kDa SSA/Ro and deubiquitinating enzyme UnpEL: a clue to function. Interaction between 52 kDa SSA/Ro and deubiquitinating enzyme UnpEL: a clue to function. Int. J. Biochem. Cell Biol. (1357-2725) 2001 Di Donato F., Chan EK., Askanase AD., Miranda-Carus M., Buyon JP. jill.buyon@med.nyu.edu 25-Jun-2008: Contacted by JYOTI. A fetal heart cDNA library, (constructed from the total RNA isolated from 18 fetal hearts), was used. davet yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Di Donato F., Chan EK., Askanase AD., Miranda-Carus M., Buyon JP. jill.buyon@med.nyu.edu Accepted 2008-JUN-13 by DAVET. A fetal heart cDNA library, (constructed from the total RNA isolated from 18 fetal hearts), was used. Int. J. Biochem. Cell Biol. (1357-2725) 2001 davet ef1d_human Elongation factor 1-delta EEF1D EF1D Antigen NY-CO-4 protein protein human Homo sapiens Human MATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI CE778D6D5D09BD6C ro52_human E3 ubiquitin-protein ligase TRIM21 TRIM21 RNF81 Sjoegren syndrome type A antigen Ro(SS-A) 52 kDa ribonucleoprotein autoantigen Ro/SS-A Tripartite motif-containing protein 21 RING finger protein 81 52 kDa Ro protein RO52 SSA1 RING-type E3 ubiquitin transferase TRIM21 protein protein human Homo sapiens Human MASAARLTMMWEEVTCPICLDPFVEPVSIECGHSFCQECISQVGKGGGSVCPVCRQRFLLKNLRPNRQLANMVNNLKEISQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY DDFF2944AFC629FB q13107-1 Ubiquitin carboxyl-terminal hydrolase 4 USP4 UNP UNPH Deubiquitinating enzyme 4 Ubiquitin-specific-processing protease 4 Ubiquitous nuclear protein homolog UnpEL Ubiquitin thioesterase 4 protein protein human Homo sapiens Human MAEGGGCRERPDAETQKSELGPLMRTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPIDNSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLELKLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTVQDAGLYQGQVLVIEPQNEDGTWPRQTLQSKSSTAPSRNFTTSPKSSASPYSSVSASLIANGDSTSTCGMHSSGVSRGGSGFSASYNCQEPPSSHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRVMEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVADVYNHRFHKIFQMDEGLNHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLESLYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGEDEPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETRRLYYDEQESEAYEKHVSMLQPQKKKKTTVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFDLWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVYDLIAVSNHYGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEFYKTPSLSSSGSSDGGTRPSSSQQGFGDDEACSMDTN 63055C9ADFE36713 tnni3_human Troponin I, cardiac muscle Cardiac troponin I TNNI3 TNNC1 protein protein human Homo sapiens Human MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES DIP protein P19429 original sequence version: 120 20A804F8C24AE1B0 trim21-usp4 3 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association trim21-usp4-1 3 5 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association This interaction was not reconfirmed in the directed yeast two-hybrid assay. trim21-eef1d 3 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association This interaction was not reconfirmed in the directed yeast two-hybrid assay. trim21-usp4-2 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Table 1 UnpEL did not interact with the 52 beta isoform of TRIM21 according to the authors. trim21-usp4-3 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association UnpEL did not interact with the 52 beta isoform of TRIM21 according to the authors. The interaction of 52 kDa SSA/Ro and UnpEL gave a 20-fold higher signal than the negative control Table 2