IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Functional analysis of the protein-interacting domains of chloroplast SRP43. Functional analysis of the protein-interacting domains of chloroplast SRP43. J. Biol. Chem. (0021-9258) 2001 Jonas-Straube E., Hutin C., Hoffman NE., Schunemann D. It was not possible to unambigiously map the protein identified in the text as LHCP. Therefore interactions involving this protein were not curated. schuenemann@bio3.rwth-aachen.de 16-Oct-2008: Contacted by JYOTI. jyoti yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Jonas-Straube E., Hutin C., Hoffman NE., Schunemann D. It was not possible to unambigiously map the protein identified in the text as LHCP. Therefore interactions involving this protein were not curated. schuenemann@bio3.rwth-aachen.de Accepted 2008-OCT-22 by JYOTI. 16-Oct-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2001 jyoti Functional analysis of the protein-interacting domains of chloroplast SRP43. Functional analysis of the protein-interacting domains of chloroplast SRP43. J. Biol. Chem. (0021-9258) 2001 Jonas-Straube E., Hutin C., Hoffman NE., Schunemann D. It was not possible to unambigiously map the protein identified in the text as LHCP. Therefore interactions involving this protein were not curated. schuenemann@bio3.rwth-aachen.de 16-Oct-2008: Contacted by JYOTI. jyoti in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Jonas-Straube E., Hutin C., Hoffman NE., Schunemann D. It was not possible to unambigiously map the protein identified in the text as LHCP. Therefore interactions involving this protein were not curated. schuenemann@bio3.rwth-aachen.de Accepted 2008-OCT-22 by JYOTI. 16-Oct-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2001 jyoti sr43c_arath Signal recognition particle 43 kDa protein, chloroplastic CAO T30B22.25 At2g47450 Chromo protein SRP43 CPSRP43 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQKVFLAMDTCALVIHQSLSRIKLSPPKSSSSSSSAFSPESLPIRRIELCFRGAICAAVQRNYEETTSSVEEAEEDDESSSSYGEVNKIIGSRTAGEGAMEYLIEWKDGHSPSWVPSSYIAADVVSEYETPWWTAARKADEQALSQLLEDRDVDAVDENGRTALLFVAGLGSDKCVRLLAEAGADLDHRDMRGGLTALHMAAGYVRPEVVEALVELGADIEVEDERGLTALELAREILKTTPKGNPMQFGRRIGLEKVINVLEGQVFEYAEVDEIVEKRGKGKDVEYLVRWKDGGDCEWVKGVHVAEDVAKDYEDGLEYAVAESVIGKRVGDDGKTIEYLVKWTDMSDATWEPQDNVDSTLVLLYQQQQPMNE DIP protein O22265 original sequence version: 82 DIP protein O22265 original sequence version: 98 F75ED9C7046A1441 sr54c_arath Signal recognition particle 54 kDa protein, chloroplastic FFC At5g03940 F8F6_150 FFC CPSRP54 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEALQFSSVNRVPCTLSCTGNRRIKAAFSSAFTGGTINSASLSSSRNLSTREIWSWVKSKTVVGHGRYRRSQVRAEMFGQLTGGLEAAWSKLKGEEVLTKDNIAEPMRDIRRALLEADVSLPVVRRFVQSVSDQAVGMGVIRGVKPDQQLVKIVHDELVKLMGGEVSELQFAKSGPTVILLAGLQGVGKTTVCAKLACYLKKQGKSCMLIAGDVYRPAAIDQLVILGEQVGVPVYTAGTDVKPADIAKQGLKEAKKNNVDVVIMDTAGRLQIDKGMMDELKDVKKFLNPTEVLLVVDAMTGQEAAALVTTFNVEIGITGAILTKLDGDSRGGAALSVKEVSGKPIKLVGRGERMEDLEPFYPDRMAGRILGMGDVLSFVEKATEVMRQEDAEDLQKKIMSAKFDFNDFLKQTRAVAKMGSMTRVLGMIPGMGKVSPAQIREAEKNLLVMEAMIEVMTPEERERPELLAESPERRKRIAKDSGKTEQQVSALVAQIFQMRVKMKNLMGVMEGGSIPALSGLEDALKAEQKAPPGTARRKRKADSRKKFVESASSKPGPRGFGSGN DIP protein P37107 original sequence version: 105 423F7285FB9063E4 cao 1 3 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table II When the third ankyrin repeat is also removed (cpSRP43-(61-193)), dimerization is essentially abolished. No binding was detected between cpSRP43 and cpSRP43-(61-159) containing the first ankyrin repeat only. These data together with the results from Table II indicate that the third and fourth ankyrin repeats each are capable of mediating the dimerization of cpSRP43. The authors concluded that "the third and fourth ankyrin repeats each are capable of mediating the dimerization of cpSRP43". There is a discrepency in lengths of SPR43 cited in the text (376 amino acids) and the UniProt record (373 amino acids). The UniProt record length is presented in this instance. ffc-cao 1 4 unspecified role unspecified role bait bait c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Table III Table IV these binding data indicate that cpSRP54 binds cpSRP43 via the chromodomains The authors concluded that "that both chromodomains" of SPR43 "contain essential elements for binding cpSRP54". There is a discrepency in lengths of SPR43 cited in the text (376 amino acids) and the UniProt record (373 amino acids). The UniProt record length is presented in this instance. cao-1 2 3 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Figure 3A First, we verified that the region of cpSRP43 containing the third and fourth ankyrin repeat (cpSRP43-(190-258)) is able to form homodimers. cao-ffc 2 4 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 3 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 3B Fig. 3B shows that cpSRP54-(371-564) is specifically co-precipitated by GST-cpSRP43 and GST-cpSRP43-(259-350) and not by the unfused GST protein The authors noted that "the binding of cpSRP54-(371-564) to GST-cpSRP43-(259-350) is significantly weaker than the binding to full-length GST-cpSRP43".