IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Tat1, a novel sulfate transporter specifically expressed in human male germ cells and potentially linked to rhogtpase signaling. Tat1, a novel sulfate transporter specifically expressed in human male germ cells and potentially linked to rhogtpase signaling. J. Biol. Chem. (0021-9258) 2001 Toure A., Morin L., Pineau C., Becq F., Dorseuil O., Gacon G. gacon@cochin.inserm.fr 27-Aug-2008: Contacted by JYOTI. davet yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Toure A., Morin L., Pineau C., Becq F., Dorseuil O., Gacon G. gacon@cochin.inserm.fr Accepted 2008-AUG-28 by DAVET. J. Biol. Chem. (0021-9258) 2001 davet Tat1, a novel sulfate transporter specifically expressed in human male germ cells and potentially linked to rhogtpase signaling. Tat1, a novel sulfate transporter specifically expressed in human male germ cells and potentially linked to rhogtpase signaling. J. Biol. Chem. (0021-9258) 2001 Toure A., Morin L., Pineau C., Becq F., Dorseuil O., Gacon G. gacon@cochin.inserm.fr 27-Aug-2008: Contacted by JYOTI. davet in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Toure A., Morin L., Pineau C., Becq F., Dorseuil O., Gacon G. gacon@cochin.inserm.fr Accepted 2008-AUG-28 by DAVET. 27-Aug-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2001 davet s26a8_human Testis anion transporter 1 SLC26A8 TAT1 Anion exchange transporter Solute carrier family 26 member 8 protein protein human Homo sapiens Human MAQLERSAISGFSSKSRRNSFAYDVKREVYNEETFQQEHKRKASSSGNMNINITTFRHHVQCRCSWHRFLRCVLTIFPFLEWMCMYRLKDWLLGDLLAGISVGLVQVPQGLTLSLLARQLIPPLNIAYAAFCSSVIYVIFGSCHQMSIGSFFLVSALLINVLKVSPFNNGQLVMGSFVKNEFSAPSYLMGYNKSLSVVATTTFLTGIIQLIMGVLGLGFIATYLPESAMSAYLAAVALHIMLSQLTFIFGIMISFHAGPISFFYDIINYCVALPKANSTSILVFLTVVVALRINKCIRISFNQYPIEFPMELFLIIGFTVIANKISMATETSQTLIDMIPYSFLLPVTPDFSLLPKIILQAFSLSLVSSFLLIFLGKKIASLHNYSVNSNQDLIAIGLCNVVSSFFRSCVFTGAIARTIIQDKSGGRQQFASLVGAGVMLLLMVKMGHFFYTLPNAVLAGIILSNVIPYLETISNLPSLWRQDQYDCALWMMTFSSSIFLGLDIGLIISVVSAFFITTVRSHRAKILLLGQIPNTNIYRSINDYREIITIPGVKIFQCCSSITFVNVYYLKHKLLKEVDMVKVPLKEEEIFSLFNSSDTNLQGGKICRCFCNCDDLEPLPRILYTERFENKLDPEASSINLIHCSHFESMNTSQTASEDQVPYTVSSVSQKNQGQQYEEVEEVWLPNNSSRNSSPGLPDVAESQGRRSLIPYSDASLLPSVHTIILDFSMVHYVDSRGLVVLRQICNAFQNANILILIAGCHSSIVRAFERNDFFDAGITKTQLFLSVHDAVLFALSRKVIGSSELSIDESETVIRETYSETDKNDNSRYKMSSSFLGSQKNVSPGFIKIQQPVEEESELDLELESEQEAGLGLDLDLDRELEPEMEPKAETETKTQTEMEPQPETEPEMEPNPKSRPRAHTFPQQRYWPMYHPSMASTQSQTQTRTWSVERRRHPMDSYSPEGNSNEDV 7F932A9CFE3A9EC4 rgap1_human Rac GTPase-activating protein 1 Male germ cell RacGap RACGAP1 KIAA1478 MGCRACGAP Protein CYK4 homolog protein protein human Homo sapiens Human MDTMMLNVRNLFEQLVRRVEILSEGNEVQFIQLAKDFEDFRKKWQRTDHELGKYKDLLMKAETERSALDVKLKHARNQVDVEIKRRQRAEADCEKLERQIQLIREMLMCDTSGSIQLSEEQKSALAFLNRGQPSSSNAGNKRLSTIDESGSILSDISFDKTDESLDWDSSLVKTFKLKKREKRRSTSRQFVDGPPGPVKKTRSIGSAVDQGNESIVAKTTVTVPNDGGPIEAVSTIETVPYWTRSRRKTGTLQPWNSDSTLNSRQLEPRTETDSVGTPQSNGGMRLHDFVSKTVIKPESCVPCGKRIKFGKLSLKCRDCRVVSHPECRDRCPLPCIPTLIGTPVKIGEGMLADFVSQTSPMIPSIVVHCVNEIEQRGLTETGLYRISGCDRTVKELKEKFLRVKTVPLLSKVDDIHAICSLLKDFLRNLKEPLLTFRLNRAFMEAAEITDEDNSIAAMYQAVGELPQANRDTLAFLMIHLQRVAQSPHTKMDVANLAKVFGPTIVAHAVPNPDPVTMLQDIKRQPKVVERLLSLPLEYWSQFMMVEQENIDPLHVIENSNAFSTPQTPDIKVSLLGPVTTPEHQLLKTPSSSSLSQRVRSTLTKNTPRFGSKSKSATNLGRQGNFFASPMLK DIP protein Q9H0H5 original sequence version: 87 DIP protein Q9H0H5 original sequence version: 144 032B7DF9CEA8F39D slc26a8-racgap1 1 4 unspecified role unspecified role prey prey n-terminus required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role bait bait c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 8A Tat1/MgcRacGAP interaction was analyzed in two-hybrid experiments and in vitro binding assays using recombinant proteins and anti-Tat1C-ter antibodies. In the yeast two-hybrid assay, Tat1 COOH-terminal region strongly interacts with the Mgc-N region (aa 1-180) but not with the GAP domain (Fig. 8A). The authors state the positions used in the protein sequence for MgcRacGAP that they had published in 1998 PMID 9497316. However the sequence in UniProtKB has an N-terminus extension by 105 AA and so the positions have been updated accordingly. slc26a8-racgap1-1 2 3 unspecified role unspecified role bait bait c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 4 unspecified role unspecified role prey prey n-terminus required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig. 8B In vitro, GST-Mgc-N fusion protein actually binds to the carboxyl-terminal domain of Tat1. The Tat1 COOH-terminal region also interacts with the full-length MgcRacGAP protein, but not with the GAP domain (Fig. 8B, lanes 2-4). These results demonstrate that MgcRacGAP interacts through its NH2-terminal region with the cytoplasmic COOH-terminal domain of Slc26a8/Tat1. The authors state the positions used in the protein sequence for MgcRacGAP that they had published in 1998 PMID 9497316. However the sequence in UniProtKB has an N-terminus extension by 105 AA and so the positions have been updated accordingly.