IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases. The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases. EMBO J. (0261-4189) 2001 imex curation Albrecht V., Ritz O., Linder S., Harter K., Kudla J. joerg.kudla@biologie.uni-ulm.de 25-Jun-2008: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Albrecht V., Ritz O., Linder S., Harter K., Kudla J. joerg.kudla@biologie.uni-ulm.de Accepted 2008-JUN-7 by ORCHARD. 25-Jun-2008: Contacted by JYOTI. EMBO J. (0261-4189) 2001 orchard Only protein-protein interactions imex curation The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases. The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases. EMBO J. (0261-4189) 2001 imex curation Albrecht V., Ritz O., Linder S., Harter K., Kudla J. joerg.kudla@biologie.uni-ulm.de 25-Jun-2008: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Albrecht V., Ritz O., Linder S., Harter K., Kudla J. joerg.kudla@biologie.uni-ulm.de Accepted 2008-JUN-7 by ORCHARD. EMBO J. (0261-4189) 2001 orchard Only protein-protein interactions imex curation The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases. The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases. EMBO J. (0261-4189) 2001 imex curation Albrecht V., Ritz O., Linder S., Harter K., Kudla J. joerg.kudla@biologie.uni-ulm.de 25-Jun-2008: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Albrecht V., Ritz O., Linder S., Harter K., Kudla J. joerg.kudla@biologie.uni-ulm.de Accepted 2008-JUN-7 by ORCHARD. 25-Jun-2008: Contacted by JYOTI. EMBO J. (0261-4189) 2001 orchard Only protein-protein interactions imex curation cipkd_arath CBL-interacting serine/threonine-protein kinase 13 PKS10 SnRK3.7 SOS2-like protein kinase PKS10 SNF1-related kinase 3.7 F13P17.2 CIPK13 At2g34180 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAQVLSTPLAIPGPTPIQFMAGLLARIVTKNTNKETSTPESPRSPRTPQGSILMDKYEIGKLLGHGSFAKVYLARNIHSGEDVAIKVIDKEKIVKSGLAGHIKREISILRRVRHPYIVHLLEVMATKTKIYIVMEYVRGGELYNTVARGRLREGTARRYFQQLISSVAFCHSRGVYHRDLKLENLLLDDKGNVKVSDFGLSVVSEQLKQEGICQTFCGTPAYLAPEVLTRKGYEGAKADIWSCGVILFVLMAGYLPFDDKNILVMYTKIYKGQFKCPKWFSPELARLVTRMLDTNPDTRITIPEIMKHRWFKKGFKHVKFYIENDKLCREDDDNDDDDSSSLSSGRSSTASEGDAEFDIKRVDSMPRPASLNAFDILSFSDLSGLFEEGGQGARFVSAAPMTKIISKLEEIAKEVKFMVRKKDWSVRLEGCREGAKGPLTIRVEIFELTPSLVVVEVKKKGGNIEEYEEFCNKELRPQLEKLMHYQADEVEEVMCLPPEIEQ C74597664AAA3C09 cnbl1_arath Calcineurin B-like protein 1 SOS3-like calcium-binding protein 5 CBL1 SCABP5 At4g17615 dl4845w FCAALL.122 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCFHSKAAKEFRGHEDPVKLASETAFSVSEVEALFELFKSISSSVVDDGLINKEEFQLALFKSRKRENIFANRIFDMFDVKRKGVIDFGDFVRSLNVFHPNASLEDKIDFTFRLYDMDCTGYIERQEVKQMLIALLCESEMKLADETIEIILDKTFEDADVNQDGKIDKLEWSDFVNKNPSLLKIMTLPYLRDITTTFPSFVFHSEVDEIAT 1C310FC449D027B1 cipk7_arath CBL-interacting serine/threonine-protein kinase 7 CIPK7 PKS7 At3g23000 SnRK3.10 SR2 SRPK1 MXC7.3 SOS2-like protein kinase PKS7 SNF1-related kinase 3.10 Serine/threonine-protein kinase SR2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MESLPQPQNQSSPATTPAKILLGKYELGRRLGSGSFAKVHLARSIESDELVAVKIIEKKKTIESGMEPRIIREIDAMRRLRHHPNILKIHEVMATKSKIYLVMELASGGELFSKVLRRGRLPESTARRYFQQLASALRFSHQDGVAHRDVKPQNLLLDEQGNLKVSDFGLSALPEHLQNGLLHTACGTPAYTAPEVISRRGYDGAKADAWSCGVILFVLLVGDVPFDDSNIAAMYRKIHRRDYRFPSWISKQAKSIIYQMLDPNPVTRMSIETVMKTNWFKKSLETSEFHRNVFDSEVEMKSSVNSITAFDLISLSSGLDLSGLFEAKKKKERRFTAKVSGVEVEEKAKMIGEKLGYVVKKKMMKKEGEVKVVGLGRGRTVIVVEAVELTVDVVVVEVKVVEGEEDDSRWSDLITELEDIVLSWHNDIM 5D6703058ACF4A75 cipk9_arath CBL-interacting serine/threonine-protein kinase 9 CIPK9 At1g01140 PKS6 SnRK3.12 F6F3.28 T25K16.13 SOS2-like protein kinase PKS6 SNF1-related kinase 3.12 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSGSRRKATPASRTRVGNYEMGRTLGEGSFAKVKYAKNTVTGDQAAIKILDREKVFRHKMVEQLKREISTMKLIKHPNVVEIIEVMASKTKIYIVLELVNGGELFDKIAQQGRLKEDEARRYFQQLINAVDYCHSRGVYHRDLKPENLILDANGVLKVSDFGLSAFSRQVREDGLLHTACGTPNYVAPEVLSDKGYDGAAADVWSCGVILFVLMAGYLPFDEPNLMTLYKRICKAEFSCPPWFSQGAKRVIKRILEPNPITRISIAELLEDEWFKKGYKPPSFDQDDEDITIDDVDAAFSNSKECLVTEKKEKPVSMNAFELISSSSEFSLENLFEKQAQLVKKETRFTSQRSASEIMSKMEETAKPLGFNVRKDNYKIKMKGDKSGRKGQLSVATEVFEVAPSLHVVELRKTGGDTLEFHKFYKNFSSGLKDVVWNTDAAAEEQKQ 5E1A9B8DD37A0E32 cipk1_arath CBL-interacting serine/threonine-protein kinase 1 CIPK1 PKS13 SnRK3.16 MKP6.6 At3g17510 SOS2-like protein kinase PKS13 SNF1-related kinase 3.16 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVRRQEEEKKAEKGMRLGKYELGRTLGEGNFGKVKFAKDTVSGHSFAVKIIDKSRIADLNFSLQIKREIRTLKMLKHPHIVRLHEVLASKTKINMVMELVTGGELFDRIVSNGKLTETDGRKMFQQLIDGISYCHSKGVFHRDLKLENVLLDAKGHIKITDFGLSALPQHFRDDGLLHTTCGSPNYVAPEVLANRGYDGAASDIWSCGVILYVILTGCLPFDDRNLAVLYQKICKGDPPIPRWLSPGARTMIKRMLDPNPVTRITVVGIKASEWFKLEYIPSIPDDDDEEEVDTDDDAFSIQELGSEEGKGSDSPTIINAFQLIGMSSFLDLSGFFEQENVSERRIRFTSNSSAKDLLEKIETAVTEMGFSVQKKHAKLRVKQEERNQKGQVGLSVTAEVFEIKPSLNVVELRKSYGDSCLYRQLYERLLKDVGTSSPEQEIVT 63DB8AF69CCAA5C9 cipkb_arath CBL-interacting serine/threonine-protein kinase 11 SOS2-like protein kinase PKS5 SNF1-related kinase 3.22 SOS3-interacting protein 4 CIPK11 PKS5 SIP4 SnRK3.22 At2g30360 T9D9.17 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPEIEIAAGSGDNNDALFGKYELGKLLGCGAFAKVFHARDRRTGQSVAVKILNKKKLLTNPALANNIKREISIMRRLSHPNIVKLHEVMATKSKIFFAMEFVKGGELFNKISKHGRLSEDLSRRYFQQLISAVGYCHARGVYHRDLKPENLLIDENGNLKVSDFGLSALTDQIRPDGLLHTLCGTPAYVAPEILSKKGYEGAKVDVWSCGIVLFVLVAGYLPFNDPNVMNMYKKIYKGEYRFPRWMSPDLKRFVSRLLDINPETRITIDEILKDPWFVRGGFKQIKFHDDEIEDQKVESSLEAVKSLNAFDLISYSSGLDLSGLFAGCSNSSGESERFLSEKSPEMLAEEVEGFAREENLRMKKKKEEEYGFEMEGQNGKFGIGICISRLNDLLVVVEARRRGGDGDCYKEMWNGKLRVQLIRVCDQTSSTNAAI 50C256B3C2B7D900 cipk2_arath CBL-interacting serine/threonine-protein kinase 2 CIPK2 At5g07070 PKS16 SnRK3.2 SOS2-like protein kinase PKS16 SNF1-related kinase 3.2 T28J14.10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MENKPSVLTERYEVGRLLGQGTFAKVYFGRSNHTNESVAIKMIDKDKVMRVGLSQQIKREISVMRIAKHPNVVELYEVMATKSRIYFVIEYCKGGELFNKVAKGKLKEDVAWKYFYQLISAVDFCHSRGVYHRDIKPENLLLDDNDNLKVSDFGLSALADCKRQDGLLHTTCGTPAYVAPEVINRKGYEGTKADIWSCGVVLFVLLAGYLPFHDTNLMEMYRKIGKADFKCPSWFAPEVKRLLCKMLDPNHETRITIAKIKESSWFRKGLHLKQKKMEKMEKQQVREATNPMEAGGSGQNENGENHEPPRLATLNAFDIIALSTGFGLAGLFGDVYDKRESRFASQKPASEIISKLVEVAKCLKLKIRKQGAGLFKLERVKEGKNGILTMDAEIFQVTPTFHLVEVKKCNGDTMEYQKLVEEDLRPALADIVWVWQGEKEKEEQLLQDEQGEQEPS 948E0CC1735C2555 o82051_sorbi Non-specific serine/threonine protein kinase SNFL3 protein protein sorbi Sorghum bicolor (Sorghum) Sorghum Sorghum vulgare MDERRTILMDRYEIGRQLGQGNFAKVYFARNLTDGQSVAIKMIDKDKITRVGLIVQIKREISIMRLVKHPNVLQLFEVMASKSKIYFVLEYAKGGELFNKISKGKFSEDVARRYFHQLISAVDYCHSRGVYHRDLKPENLLLDENENLKVSDFGLSALAESKRHDGLLHTTCGTPAYVAPEVLSRRGYDGTKADIWSCGVILFVLVAGYLPFHDPNLIEMYRKISKAEYRCPRSFSGELKDLLFRMLDPDPSTRISISRIKRSTWYRKPIEVNSAKIKHDTTRDKVCNGEATTSNSIECSNSEETRGSSSLPNLNAFDIISLSTGFDLSNLFEEKYGRREERFTTRQPAGTVFAKLNELAERLKLKIKKKENGVLKLASPKEGIKGFLELDAEIFELAPSFLLVELKKTNGDTIEYQRLVKEEIRPALKDMVWAWQSDRHQQRNESVQGEQQLSSPLPPQQ CE2245474FABA19C cipk4_arath CBL-interacting serine/threonine-protein kinase 4 CIPK4 PKS9 SnRK3.3 At4g14580 SOS2-like protein kinase PKS9 SNF1-related kinase 3.3 dl3330c FCAALL.259 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MESPYPKSPEKITGTVLLGKYELGRRLGSGSFAKVHVARSISTGELVAIKIIDKQKTIDSGMEPRIIREIEAMRRLHNHPNVLKIHEVMATKSKIYLVVEYAAGGELFTKLIRFGRLNESAARRYFQQLASALSFCHRDGIAHRDVKPQNLLLDKQGNLKVSDFGLSALPEHRSNNGLLHTACGTPAYTAPEVIAQRGYDGAKADAWSCGVFLFVLLAGYVPFDDANIVAMYRKIHKRDYRFPSWISKPARSIIYKLLDPNPETRMSIEAVMGTVWFQKSLEISEFQSSVFELDRFLEKEAKSSNAITAFDLISLSSGLDLSGLFERRKRKEKRFTARVSAERVVEKAGMIGEKLGFRVEKKEETKVVGLGKGRTAVVVEVVEFAEGLVVADVKVVVEGEEEEEEVESHWSELIVELEEIVLSWHN 7B6A34848A6F62AE cipk6_arath CBL-interacting serine/threonine-protein kinase 6 SOS2-like protein kinase PKS4 SNF1-related kinase 3.14 SOS3-interacting protein 3 CIPK6 PKS4 At4g30960 SIP3 SnRK3.14 F6I18.130 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVGAKPVENGSDGGSSTGLLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQIKREISVMRMVKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAKGRLREDVARVYFQQLISAVDFCHSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPAYVAPEVILKKGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLSSDARRLVTKLLDPNPNTRITIEKVMDSPWFKKQATRSRNEPVAATITTTEEDVDFLVHKSKEETETLNAFHIIALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVGNKFDVRKSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTALRPALKDIFWTSTPA 469728C6CB83F428 cipke_arath CBL-interacting serine/threonine-protein kinase 14 CIPK14 At5g01820 PKS24 SnRK3.15 SR1 SOS2-like protein kinase PKS24 SNF1-related kinase 3.15 Serine/threonine-protein kinase SR1 T20L15.90 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVDSDPVEFPPENRRGQLFGKYEVGKLVGCGAFAKVYHGRSTATGQSVAIKVVSKQRLQKGGLNGNIQREIAIMHRLRHPSIVRLFEVLATKSKIFFVMEFAKGGELFAKVSKGRFCEDLSRRYFQQLISAVGYCHSRGIFHRDLKPENLLLDEKLDLKISDFGLSALTDQIRPDGLLHTLCGTPAYVAPEVLAKKGYDGAKIDIWSCGIILFVLNAGYLPFNDHNLMVMYRKIYKGEFRIPKWTSPDLRRLLTRLLDTNPQTRITIEEIIHDPWFKQGYDDRMSKFHLEDSDMKLPADETDSEMGARRMNAFDIISGSPGFNLSGLFGDARKYDRVERFVSAWTAERVVERLEEIVSAENLTVAKKETWGMKIEGQKGNFAMVVEINQLTDELVMIEVRKRQRAAASGRDLWTDTLRPFFVELVHESDQTDPEPTQVHTTS 319532FEFB7244C8 cnbl5_arath Calcineurin B-like protein 5 SOS3-like calcium-binding protein 4 CBL5 SCABP4 At4g01420 F3D13.2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCVCSKQLEGRRQEDISLLASQTFFSEAEVEVLHGLFIKLTSCLSNDNLLTKEKFQFILIKNTKKRSLSAERIFGLFDMRNDGAIDFGEFVHTLNIFHPNSSPRDKAIFAFRLYDTRETGFIEPEEVKEMIIDVLEESELMLSESIIDSIVSKTFEEADWKKDGIIDLEEWENFVATYPLTLKNMTIPFLKDIPRIFPTFLR 853C31FB4060F548 cnbl4_arath Calcineurin B-like protein 4 Protein SALT OVERLY SENSITIVE 3 CBL4 SOS3 At5g24270 MOP9.8 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCSVSKKKKKNAMRPPGYEDPELLASVTPFTVEEVEALYELFKKLSSSIIDDGLIHKEEFQLALFRNRNRRNLFADRIFDVFDVKRNGVIEFGEFVRSLGVFHPSAPVHEKVKFAFKLYDLRQTGFIEREELKEMVVALLHESELVLSEDMIEVMVDKAFVQADRKNDGKIDIDEWKDFVSLNPSLIKNMTLPYLKDINRTFPSFVSSCEEEEMELQNVSS DIP protein O81223 original sequence version: 98 DF06C3973748AFF7 cnbl6_arath Calcineurin B-like protein 6 CBL6 SCABP2 dl4205c FCAALL.89 At4g16350 SOS3-like calcium-binding protein 2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMMQCLDGLKHLALILLTCCDADPPKVRQNPKDVARGTVFTVNEIEALYELFKSISKNGLIDKEQFQLVLFKMNTTRSLFADRVFDLFDTKNTGILDFEAFARSLSVFHPNAKFEDKIEFSFKLYDLNQQGYIKRQEVKQMVVRTLAESGMNLSDHVIESIIDKTFEEADTKLDGKIDKEEWRSLVLRHPSLLQNMSLQHLKDVTKTFPNFVFHTIVTDTPSELDG E7754ED8DF130B0E cnbl2_arath Calcineurin B-like protein 2 CBL2 SCABP1 At5g55990 MDA7.3 SOS3-like calcium-binding protein 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSQCVDGIKHLCTSVLGCFDLDLYKQSGGLGDPELLARDTVFSVSEIEALYELFKKISSAVIDDGLINKEEFQLALFKTNKKESLFADRVFDLFDTKHNGILGFEEFARALSVFHPNAPIDDKIHFSFQLYDLKQQGFIERQEVKQMVVATLAESGMNLKDTVIEDIIDKTFEEADTKHDGKIDKEEWRSLVLRHPSLLKNMTLQYLKDITTTFPSFVFHSQVEDT 342A013FC28D1445 cipkc_arath CBL-interacting serine/threonine-protein kinase 12 SOS2-like protein kinase PKS8 SNF1-related kinase 3.9 F28A21.110 CIPK12 PKS8 At4g18700 SnRK3.9 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAEKITRETSLPKERSSPQALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKEKVLKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRLKEEVARKYFQQLISAVTFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLARKGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYRGEFRCPRWFSTELTRLLSKLLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVVDDDELESDSVESDRDSAASESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFDDDGEGSRFVSGAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFELTPSLVVVEVKKKGGDKTEYEDFCNNELKPKLQNLTADDVVAEPVAVSAVDETAIPNSPTISFLPSDTE DEBD1F2EEFE4CB1E cnbl3_arath Calcineurin B-like protein 3 SOS3-like calcium-binding protein 6 CBL3 SCABP6 At4g26570 T15N24.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSQCIDGFKHVCSSFFRCFDIDIYKQSGGLGDPELLARETVFSVSEIEALYELFKKISSAVIDDGLINKEEFQLALFKTNKKESLFADRVFDLFDTKHNGILGFEEFARALSVFHPNAPIEDKIDFSFQLYDLKQQGFIERQEVKQMVVATLAESGMNLSDEIIESIIDKTFEEADTKHDGRIDKEEWRTLVLRHPSLLKNMTLQYLKDITTTFPSFVFHSQVEDT F78222E98F64983E cipk1-cbl2 2 8 unspecified role unspecified role prey prey Q8RWC9:p.Ala320Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain A P Wed May 02 15:11:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala320pro' Tue Jun 30 19:23:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala320Pro' Q8RWC9:p.Phe335Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain F P Wed May 02 16:01:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe335pro' Tue Jun 30 16:18:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe335Pro' Q8RWC9:p.Ile324Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain I D Wed May 02 16:46:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile324asp' Tue Jun 30 19:57:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile324Asp' Q8RWC9:p.Ser327Leu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S L Wed May 02 17:21:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser327leu' Tue Jun 30 19:44:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser327Leu' Q8RWC9:p.Leu332Asp mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L D Wed May 02 17:56:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu332asp' Tue Jun 30 19:47:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu332Asp' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q8RWC9:p.Pro315Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain P A Wed May 02 18:38:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro315ala' Tue Jun 30 17:50:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro315Ala' 18 unspecified role unspecified role bait bait physical association physical association Figure 1A, Figure 1B, Figure 2A, Figure 2B There is a discrepancy between the text and figure legends in this paper with regard to the Q8RWC9 protein amino acid residues which are mutated. The text states that the mutation Ile322Asp effected binding between the proteins but in the Figure 2A the Ile residue in question is at position 323. Also the text describes the mutation at position 334 as Leu to a Asp conversion , in Figure 2A this is a Phe to Pro conversion. However in the UniProt record all residues which are mutated are +1 amino acid residue to the position identified in the text and the coordinates from the UniProt record are taken as the correct ones and presented here cipk1-cbl2-1 3 18 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Figure 1C, Figure 7B As shown in Figure 1C (right panel), full-length AtCIPK1 (Kin), KinD1 and KinD4, but not KinD6 and KinD9, interacted with AtCBL2-His6 in vitro cipk1-cbl2-2 1 8 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q8RWC9:p.Phe335Pro mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain F P Wed May 02 15:29:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe335pro' Tue Jun 30 16:42:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe335Pro' 18 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Figure 2C In the UniProt record all residues for protein Q8RWC9 which are mutated are +1 amino acid residue to the position identified in the text and the coordinates from the UniProt record are taken as the correct ones and presented here cipk1-cbl1-1 2 5 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 3, Figure 7A cipk1-cbl3 2 20 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 3 The pdf would not let me cut and paste source text cipk1-cbl4 2 16 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 3 cipk1-cbl5 2 15 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 3 cipk1-cbl6 2 8 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role bait bait physical association physical association Figure 3 snfl3-cbl2-3 2 11 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 18 unspecified role unspecified role bait bait physical association physical association Figure 5 cipk2-cbl1 2 10 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Figure 7A atsrpk1-cbl1 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk9-cbl1-1 2 5 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk11-cbl1 2 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text at5g01820-cbl1 2 5 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk2-cbl2 2 18 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Figure 7A The pdf would not let me cut and paste source text at4g14580-cbl2-1 2 12 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text atsrpk1-cbl2-2 2 18 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk9-cbl2 2 7 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk11-cbl2-1 2 9 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text at4g18700-cbl2-2 2 19 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk13-cbl2-3 2 4 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text at5g01820-cbl2 2 18 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association Figure 7A The pdf would not let me cut and paste source text at4g30960-cbl2-3 2 13 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk2-cbl3 2 10 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text at4g14580-cbl3 2 12 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text at4g30960-cbl3-1 2 13 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text atsrpk1-cbl3-2 2 6 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk9-cbl3-3 2 20 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk11-cbl3 2 20 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Figure 7A The pdf would not let me cut and paste source text at4g18700-cbl3-1 2 19 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk13-cbl3-2 2 4 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text at5g01820-cbl3-3 2 14 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk2-cbl4 2 16 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Figure 7A The pdf would not let me cut and paste source text atsrpk1-cbl4-1 2 6 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text cip9-cbl4 2 7 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text at5g01820-cbl4-1 2 14 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk2-cbl5-1 2 15 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Figure 7A The pdf would not let me cut and paste source text atsrpk1-cbl5-2 2 6 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk11-cbl5-3 2 9 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text at5g01820-cbl5-4 2 14 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text atsrpk1-cbl6-1 2 6 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk11-cbl6-2 2 17 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Figure 7A The pdf would not let me cut and paste source text at5g01820-cbl6-3 2 14 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait physical association physical association Figure 7A The pdf would not let me cut and paste source text cipk13-cbl2 3 4 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 18 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Figure 7B at5g01820-cbl2-1 3 14 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 18 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Figure 7B