IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SOS3 function in plant salt tolerance requires N-myristoylation and calcium binding. SOS3 function in plant salt tolerance requires N-myristoylation and calcium binding. Plant Cell (1040-4651) 2000 imex curation Ishitani M., Liu J., Halfter U., Kim CS., Shi W., Zhu JK. Note: The data in Figure 8 was not curated as it is not clear from the Figure if S0S3 or SOS2 is responsible for phosphorylation of the peptide substrate. jkzhu@ag.arizona.edu 27-Aug-2008: Contacted by JYOTI. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ishitani M., Liu J., Halfter U., Kim CS., Shi W., Zhu JK. Note: The data in Figure 8 was not curated as it is not clear from the Figure if S0S3 or SOS2 is responsible for phosphorylation of the peptide substrate. jkzhu@ag.arizona.edu Accepted 2008-AUG-22 by JYOTI. 27-Aug-2008: Contacted by JYOTI. Plant Cell (1040-4651) 2000 jyoti Only protein-protein interactions imex curation SOS3 function in plant salt tolerance requires N-myristoylation and calcium binding. SOS3 function in plant salt tolerance requires N-myristoylation and calcium binding. Plant Cell (1040-4651) 2000 imex curation Ishitani M., Liu J., Halfter U., Kim CS., Shi W., Zhu JK. Note: The data in Figure 8 was not curated as it is not clear from the Figure if S0S3 or SOS2 is responsible for phosphorylation of the peptide substrate. jkzhu@ag.arizona.edu 27-Aug-2008: Contacted by JYOTI. jyoti Only protein-protein interactions in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ishitani M., Liu J., Halfter U., Kim CS., Shi W., Zhu JK. Note: The data in Figure 8 was not curated as it is not clear from the Figure if S0S3 or SOS2 is responsible for phosphorylation of the peptide substrate. jkzhu@ag.arizona.edu Accepted 2008-AUG-22 by JYOTI. Plant Cell (1040-4651) 2000 jyoti Only protein-protein interactions imex curation cnbl4_arath Calcineurin B-like protein 4 Protein SALT OVERLY SENSITIVE 3 CBL4 SOS3 At5g24270 MOP9.8 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCSVSKKKKKNAMRPPGYEDPELLASVTPFTVEEVEALYELFKKLSSSIIDDGLIHKEEFQLALFRNRNRRNLFADRIFDVFDVKRNGVIEFGEFVRSLGVFHPSAPVHEKVKFAFKLYDLRQTGFIEREELKEMVVALLHESELVLSEDMIEVMVDKAFVQADRKNDGKIDIDEWKDFVSLNPSLIKNMTLPYLKDINRTFPSFVSSCEEEEMELQNVSS DIP protein O81223 original sequence version: 98 DF06C3973748AFF7 cipko_arath CBL-interacting serine/threonine-protein kinase 24 SNF1-related kinase 3.11 Protein SALT OVERLY SENSITIVE 2 CIPK24 SnRK3.11 SOS2 At5g35410 F6I13.1 K21B8.3 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTKKMRRVGKYEVGRTIGEGTFAKVKFARNTDTGDNVAIKIMAKSTILKNRMVDQIKREISIMKIVRHPNIVRLYEVLASPSKIYIVLEFVTGGELFDRIVHKGRLEESESRKYFQQLVDAVAHCHCKGVYHRDLKPENLLLDTNGNLKVSDFGLSALPQEGVELLRTTCGTPNYVAPEVLSGQGYDGSAADIWSCGVILFVILAGYLPFSETDLPGLYRKINAAEFSCPPWFSAEVKFLIHRILDPNPKTRIQIQGIKKDPWFRLNYVPIRAREEEEVNLDDIRAVFDGIEGSYVAENVERNDEGPLMMNAFEMITLSQGLNLSALFDRRQDFVKRQTRFVSRREPSEIIANIEAVANSMGFKSHTRNFKTRLEGLSSIKAGQLAVVIEIYEVAPSLFMVDVRKAAGETLEYHKFYKKLCSKLENIIWRATEGIPKSEILRTITF DIP protein Q9LDI3 original sequence version: 90 99FDF3A778E1093D calcium(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) sos3 2 5 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 5 SOS3 Binds 45Ca2+. The authors stated the following "the central EF-hand of SOS3 is necessary for interaction with SOS2" (amino acids 43-45) however they also conclude that the "results indicate that SOS3 N-terminal and C-terminal regions also contribute to the interaction with SOS2". sos3-sos2-4 1 3 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey physical association physical association Figure 7 To determine whether the sos3-1 mutation affects SOS3 interaction with SOS2, we conducted a yeast two-hybrid assay, using the mutant SOS3ΔEF as the bait and pACT-SOS2 as the prey. Figure 7 shows that unlike the wild-type SOS3, SOS3ΔEF was not capable of interacting with SOS2.