IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Arabidopsis Cdc2a-interacting protein ICK2 is structurally related to ICK1 and is a potent inhibitor of cyclin-dependent kinase activity in vitro. The Arabidopsis Cdc2a-interacting protein ICK2 is structurally related to ICK1 and is a potent inhibitor of cyclin-dependent kinase activity in vitro. Plant J. (0960-7412) 2000 imex curation Lui H., Wang H., Delong C., Fowke LC., Crosby WL., Fobert PR. There appear to be three Arabidopsis cyclin D3 (CYCD3) genes in Arabidopsis and it is not possible from the information provided to identify which of these was used in the study. Therefore interactions involving this protein were not curated. pfobert@pbi.nrc.ca 17-April-2008: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lui H., Wang H., Delong C., Fowke LC., Crosby WL., Fobert PR. There appear to be three Arabidopsis cyclin D3 (CYCD3) genes in Arabidopsis and it is not possible from the information provided to identify which of these was used in the study. Therefore interactions involving this protein were not curated. pfobert@pbi.nrc.ca Accepted 2008-MAR-19 by ORCHARD. 17-April-2008: Contacted by JYOTI. Plant J. (0960-7412) 2000 orchard Only protein-protein interactions imex curation krp1_arath Cyclin-dependent kinase inhibitor 1 KRP1 ICK1 F26B6.8 At2g23430 KIP-related protein 1 Inhibitor/interactor of CDK protein 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVRKYRKAKGIVEAGVSSTYMQLRSRRIVYVRSEKSSSVSVVGDNGVSSSCSGSNEYKKKELIHLEEEDKDGDTETSTYRRGTKRKLFENLREEEKEELSKSMENYSSEFESAVKESLDCCCSGRKTMEETVTAEEEEKAKLMTEMPTESEIEDFFVEAEKQLKEKFKKKYNFDFEKEKPLEGRYEWVKLE 0477A91E277C46B2 krp5_arath Cyclin-dependent kinase inhibitor 5 KRP5 ICK3 K7P8.10 At3g24810 KIP-related protein 5 Inhibitor/interactor of CDK protein 3 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGKYIKKSKVAGAVSVKDKSHPPALGFRTRAAAAKNLALHRLRSHSDEADSFNYLQLRSRRLVKLPLLTNTRKQQKQQLIPSVNQCQTKNPRASSGPAKKLEPDTTTEEACGDNERISRSDCNFGDKGFDLESENRSMISDSKSIQSEIEDFFASAEQQQQRFFIQKYNFDIVSDNPLPGRYEWVKVMP A66041310E8DDED6 krp2_arath Cyclin-dependent kinase inhibitor 2 KRP2 ICK2 T3A5.10 At3g50630 KIP-related protein 2 Inhibitor/interactor of CDK protein 2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAAVRRRERDVVEENGVTTTTVKRRKMEEEVDLVESRIILSPCVQATNRGGIVARNSAGASETSVVIVRRRDSPPVEEQCQIEEEDSSVSCCSTSEEKSKRRIEFVDLEENNGDDRETETSWIYDDLNKSEESMNMDSSSVAVEDVESRRRLRKSLHETVKEAELEDFFQVAEKDLRNKLLECSMKYNFDFEKDEPLGGGRYEWVKLNP 0E5EEFE9ED256B53 ckb11_arath Cyclin-dependent kinase B1-1 CDKB1-1 At3g54180 F24B22.140 CDC2B Cell division control protein 2 homolog B protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEKYEKLEKVGEGTYGKVYKAMEKGTGKLVALKKTRLEMDEEGIPPTALREISLLQMLSTSIYVVRLLCVEHVHQPSTKSQSTKSNLYLVFEYLDTDLKKFIDSYRKGPNPKPLEPFLIQKLMFQLCKGVAHCHSHGVLHRDLKPQNLLLVKDKELLKIADLGLGRAFTVPLKSYTHEIVTLWYRAPEVLLGSTHYSTGVDMWSVGCIFAEMVRRQALFPGDSEFQQLLHIFRLLGTPTEQQWPGVSTLRDWHVYPKWEPQDLTLAVPSLSPQGVDLLTKMLKYNPAERISAKTALDHPYFDSLDKSQF 8FFCD006A9A4A75B cdka1_arath Cyclin-dependent kinase A-1 CDKA-1 Cell division control protein 2 homolog A CDC2 At3g48750 CDC2A T21J18.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDQYEKVEKIGEGTYGVVYKARDKVTNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHSNIVKLQDVVHSEKRLYLVFEYLDLDLKKHMDSTPDFSKDLHMIKTYLYQILRGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGSHHYSTPVDIWSVGCIFAEMISQKPLFPGDSEIDQLFKIFRIMGTPYEDTWRGVTSLPDYKSAFPKWKPTDLETFVPNLDPDGVDLLSKMLLMDPTKRINARAALEHEYFKDLGGMP B5FAE55FA9EC366E cdka_1-krp1 1 2 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait physical association physical association Table 1 These results indicate that ICK1 and ICK2 specifically interact with Cdc2aAT, while ICK3 interacts with Cdc2aAt as well as Cdc2bAt cdka_1-krp2 1 4 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait physical association physical association Table 1 These results indicate that ICK1 and ICK2 specifically interact with Cdc2aAT, while ICK3 interacts with Cdc2aAt as well as Cdc2bAt cdka_1-krp5 1 3 unspecified role unspecified role prey prey n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait physical association physical association Table 1 These results indicate that ICK1 and ICK2 specifically interact with Cdc2aAT, while ICK3 interacts with Cdc2aAt as well as Cdc2bAt ckb11-krp5-1 1 3 unspecified role unspecified role prey prey n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait physical association physical association Table 1 These results indicate that ICK1 and ICK2 specifically interact with Cdc2aAT, while ICK3 interacts with Cdc2aAt as well as Cdc2bAt