IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A new D-type cyclin of Arabidopsis thaliana expressed during lateral root primordia formation. A new D-type cyclin of Arabidopsis thaliana expressed during lateral root primordia formation. Planta (0032-0935) 1999 De Veylder L., de Almeida Engler J., Burssens S., Manevski A., Lescure B., Van Montagu M., Engler G., Inze D. diinz@gengenp.rug.ac.be 17-April-2008: Contacted by JYOTI. A cDNA ibrary was constructed using RNA isolated from A. thaliana cell suspensions harvested at 20 h, 3, 7, and 10 d after dilution in new medium. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined De Veylder L., de Almeida Engler J., Burssens S., Manevski A., Lescure B., Van Montagu M., Engler G., Inze D. diinz@gengenp.rug.ac.be Accepted 2008-MAR-15 by JYOTI. 17-April-2008: Contacted by JYOTI. A cDNA ibrary was constructed using RNA isolated from A. thaliana cell suspensions harvested at 20 h, 3, 7, and 10 d after dilution in new medium. Planta (0032-0935) 1999 A new D-type cyclin of Arabidopsis thaliana expressed during lateral root primordia formation. A new D-type cyclin of Arabidopsis thaliana expressed during lateral root primordia formation. Planta (0032-0935) 1999 De Veylder L., de Almeida Engler J., Burssens S., Manevski A., Lescure B., Van Montagu M., Engler G., Inze D. diinz@gengenp.rug.ac.be 17-April-2008: Contacted by JYOTI. A cDNA ibrary was constructed using RNA isolated from A. thaliana cell suspensions harvested at 20 h, 3, 7, and 10 d after dilution in new medium. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence De Veylder L., de Almeida Engler J., Burssens S., Manevski A., Lescure B., Van Montagu M., Engler G., Inze D. diinz@gengenp.rug.ac.be Accepted 2008-MAR-15 by JYOTI. 17-April-2008: Contacted by JYOTI. A cDNA ibrary was constructed using RNA isolated from A. thaliana cell suspensions harvested at 20 h, 3, 7, and 10 d after dilution in new medium. Planta (0032-0935) 1999 cdka1_arath Cyclin-dependent kinase A-1 CDKA-1 Cell division control protein 2 homolog A CDC2 At3g48750 CDC2A T21J18.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDQYEKVEKIGEGTYGVVYKARDKVTNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHSNIVKLQDVVHSEKRLYLVFEYLDLDLKKHMDSTPDFSKDLHMIKTYLYQILRGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGSHHYSTPVDIWSVGCIFAEMISQKPLFPGDSEIDQLFKIFRIMGTPYEDTWRGVTSLPDYKSAFPKWKPTDLETFVPNLDPDGVDLLSKMLLMDPTKRINARAALEHEYFKDLGGMP B5FAE55FA9EC366E ckb21_arath Cyclin-dependent kinase B2-1 CDKB2-1 F14G6.14 At1g76540 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDEGVIAVSAMDAFEKLEKVGEGTYGKVYRAREKATGKIVALKKTRLHEDEEGVPSTTLREISILRMLARDPHVVRLMDVKQGLSKEGKTVLYLVFEYMDTDVKKFIRSFRSTGKNIPTQTIKSLMYQLCKGMAFCHGHGILHRDLKPHNLLMDPKTMRLKIADLGLARAFTLPMKKYTHEILTLWYRAPEVLLGATHYSTAVDMWSVGCIFAELVTNQAIFQGDSELQQLLHIFKLFGTPNEEMWPGVSTLKNWHEYPQWKPSTLSSAVPNLDEAGVDLLSKMLQYEPAKRISAKMAMEHPYFDDLPEKSSL 7E0EBD7A553DB9C9 cdka1-cycd4_1 2 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association One of the isolated clones (LDV59) contained a gene encoding a protein showing similarity to the previously identifed D-type cyclins of A. thaliana. This new cyclin gene (GenBank accession number AJ131636) was denoted CYCD4;1 The authors identify the prey protein by the GenBank accession number AJ131636, the UniProtKB record differs from this by a Valine amino acid residue (instead of an Alanine residue) at position 229 in the amino acid sequence. cdka1-cycd4_1-1 1 3 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association Specific interaction of CDC2aAt with LDV59. Figure 1