UniProt UniProt http://www.uniprot.org P-CIP1, a novel protein that interacts with the cytosolic domain of peptidylglycine alpha-amidating monooxygenase, is associated with endosomes. P-CIP1, a novel protein that interacts with the cytosolic domain of peptidylglycine alpha-amidating monooxygenase, is associated with endosomes. J. Biol. Chem. (0021-9258) 1998 imex curation Chen L., Johnson RC., Milgram SL. AtT-20 cell lines stably expressing PAM-1, PAM-1/936, or PAM-1/Delta 928-945 have been used. Immunoprecipitated PAM-1 proteins have been incubated with bacterially expressed P-CIP1. Only protein-protein interactions crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Chen L., Johnson RC., Milgram SL. co-localization experiments fig 6 and fig 7 showing endogenous CHO proteins (TGN38, SCAMps, RAB11) not curated since the proteins are not in SP. AtT-20 cell lines stably expressing PAM-1, PAM-1/936, or PAM-1/Delta 928-945 have been used. Immunoprecipitated PAM-1 proteins have been incubated with bacterially expressed P-CIP1. Accepted 2007-OCT-25 by JYOTI. J. Biol. Chem. (0021-9258) 1998 Only protein-protein interactions imex curation P-CIP1, a novel protein that interacts with the cytosolic domain of peptidylglycine alpha-amidating monooxygenase, is associated with endosomes. P-CIP1, a novel protein that interacts with the cytosolic domain of peptidylglycine alpha-amidating monooxygenase, is associated with endosomes. J. Biol. Chem. (0021-9258) 1998 imex curation Chen L., Johnson RC., Milgram SL. AtT-20 cell lines stably expressing PAM-1, PAM-1/936, or PAM-1/Delta 928-945 have been used. Immunoprecipitated PAM-1 proteins have been incubated with bacterially expressed P-CIP1. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Chen L., Johnson RC., Milgram SL. AtT-20 cell lines stably expressing PAM-1, PAM-1/936, or PAM-1/Delta 928-945 have been used. Immunoprecipitated PAM-1 proteins have been incubated with bacterially expressed P-CIP1. Accepted 2007-OCT-25 by JYOTI. J. Biol. Chem. (0021-9258) 1998 Only protein-protein interactions imex curation amd_rat Peptidylglycine alpha-amidating monooxygenase Pam protein protein rat Rattus norvegicus (Rat) Rat MAGRARSGLLLLLLGLLALQSSCLAFRSPLSVFKRFKETTRSFSNECLGTIGPVTPLDASDFALDIRMPGVTPKESDTYFCMSMRLPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKDCSGVSVHLTRVPQPLIAGMYLMMSVDTVIPPGEKVVNADISCQYKMYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQNPQLPQAFYPVEHPVDVTFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKYALSFMTCTKNVAPDMFRTIPAEANIPIPVKPDMVMMHGHHKEAENKEKSALMQQPKQGEEEVLEQGDFYSLLSKLLGEREDVHVHKYNPTEKTESGSDLVAEIANVVQKKDLGRSDAREGAEHEEWGNAILVRDRIHRFHQLESTLRPAESRAFSFQQPGEGPWEPEPSGDFHVEEELDWPGVYLLPGQVSGVALDSKNNLVIFHRGDHVWDGNSFDSKFVYQQRGLGPIEEDTILVIDPNNAEILQSSGKNLFYLPHGLSIDTDGNYWVTDVALHQVFKLDPHSKEGPLLILGRSMQPGSDQNHFCQPTDVAVEPSTGAVFVSDGYCNSRIVQFSPSGKFVTQWGEESSGSSPRPGQFSVPHSLALVPHLDQLCVADRENGRIQCFKTDTKEFVREIKHASFGRNVFAISYIPGFLFAVNGKPYFGDQEPVQGFVMNFSSGEIIDVFKPVRKHFDMPHDIVASEDGTVYIGDAHTNTVWKFTLTEKMEHRSVKKAGIEVQEIKEAEAVVEPKVENKPTSSELQKMQEKQKLSTEPGSGVSVVLITTLLVIPVLVLLAIVMFIRWKKSRAFGDHDRKLESSSGRVLGRFRGKGSGGLNLGNFFASRKGYSRKGFDRVSTEGSDQEKDEDDGTESEEEYSAPLPKPAPSS 7233021BEBEFD9B9 rasf9_rat Ras association domain-containing protein 9 Rassf9 Pcip1 Pamci Peptidylglycine alpha-amidating monooxygenase COOH-terminal interactor PAM COOH-terminal interactor protein 1 protein protein rat Rattus norvegicus (Rat) Rat MAPFGRNLLKTRHKNRSPTKDMDPEEKEIVVWVCQEEKIVCGLTKRTTSIDVIQALLEEHEATFGEKRFLLGKASDYCIVEKWRGSERALPPLTRILKLWKAWGDEQPNMQFVLVKTDAFLPVPLWRTAETKLVQNNEKPWELSPANYMKTLPPDKQKRIVRKTFRKLAKIRQDTGSHDRDNMECLVHLIISQDHTIHQQVQRMKELDMEIEKCEAKIHLDRIGNDGADYVQEAYLMPRSSEEEQKLDFQSEDNQTLEDLNDGEGVSQLEEQLQYYRALIDKLSAEIEREVKGAGTDGSEDMEGAAACELENSDLESVKCDLEKSMKAGLKIHSHLSGIQREIKYSDSLLQMKAREYELLAKEFSSLHISSKDGCQGKENRGKEAEASSSNGEIPPLTQRVFNTYTNDTDSDTGISSNHSQDSETTLGDVLLLAT 582AF0C52176E9B1 pam-pamci-1 2 3 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain mouse-att_20 Mus musculus att_20 Mouse pituitary tumour 4 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association 1 PAM-1 fragments 1-936 and DEL928-945 did not interact. pam-pamci-2 1 3 unspecified role unspecified role neutral component neutral component crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 4 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. colocalization colocalization 6a Co-localization occured in perinuclear vesicles, which might be endosomes.