IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ sud1(+) targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast. sud1(+) targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1998 imex curation Jallepalli PV., Tien D., Kelly TJ. tom_kelly@qmail.bs.jhu.edu Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Jallepalli PV., Tien D., Kelly TJ. tom_kelly@qmail.bs.jhu.edu Accepted 2007-APR-7 by DAVET. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1998 Only protein-protein interactions imex curation pop2_schpo WD repeat-containing protein pop2 Proteolysis factor sud1 pop2 sud1 SPAC4D7.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSLSRCPTDNSSSRINSSVPLINSSSPATPPESFDPQVFPSSLIHGDNLLPQDDQIASDPRSESNSCNGNTSSSLPCTDSYQYPLKHSCTPSFLRKFNESIENVSYKCLDHSPPDSVPGDFSISLVPQRNFLYSHSSLPPKIISIDRNNRIKLDNSISSNSDNFPPSPKVDTSNTVSPGSKPISEDLEDLNLQSIVQTFEDLPEGIQSYAFFQLLRSCNRQSMRLLLNECEPLLKKDILSNLPFSIVQSILLNLDIHSFLSCRLVSPTWNRILDVHTSYWKHMFSLFGFQINENDWKYANPNLNRPPFLHNDQISDDYFPEIFKRHFLNRKRWLFPSIPPSHLSFPIHVPNFMITSLLLHKDRIITTSGSGTIQIHNAITGVLEARLEGHKEGVWAVKIHENTLVSGSIDKTVRVWNIEKAKCTHIFRGHISIIRCLEILVPSRLIRHGVEIVEPDQPYIVSGSRDHTLRVWKLPKNTDPPYLPDNTNSIDRWEKNPYFVHTLIGHTDSVRTISGYGDILVSGSYDSSIRIWRVSTGECLYHLRGHSLRIYSVLYEPERNICISGSMDKSIRVWDLSTGTCKYVLEGHDAFVTLLNVFQNRLISGSADSTIRIWDLNTGKPLMVLPSNSGYISSFVSDEHKIISGNDGSVKLWDVRTGKLLRFLLTDLTKIWHVDFDAMRCVAAVQRDDQAYLEVINFSGSRP 0336A0568C152396 cdc18_schpo Cell division control protein 18 cdc18 SPBC14C8.07c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MCETPIGCHTPRRCNRFIDSAALIDCTNKTNQREHSPSFSIEIPTTPSRKRTLASSHFQTPTKRIKYELGELQEEKTDLYPNFPAQLKENKKPKLPTTPQTPKTPKRTIQIVTPKSLNRTCNPVPFATRLLQSTPHRQLFPPTPSTPSTPSYNSTAKLSLRKSYRSAGVVGRENEKSIVESFFRQHLDANAGGALYVSGAPGTGKTVLLHNVLDHVVSDYPKVNVCYINCMTINEPKAIFEKIHSKIVKEEILENEDHHINFQCELESHFTQSANELYNPVIIVLDEMDHLIAREQQVLYTLFEWPSRPTSRLILVGIANALDMTDRFLPRLRTKHITPKLLSFTPYTAQEISTIIKARLKTAATTSEKNNPFTPIKSISEVSDDSINVVSQHADETPFIHPAAIELCARKVAASSGDLRKALDICRHAIELAEREWKAQHDNTLSSVDIPRASIAHVVRATSAMSQSASARLKNLGLQQKAILCTLVVCEKTSLSVADVFEKYSSLCLRDRLIYPLTSSEFCDVANSLETLAIIRLRTKQRNGKPQDRIISLLVPEMDVITAVGDIGTLKRFFDRR 74DBE9D5CF23504D cdc18-pop2-1 1 3 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P41411:p.[Thr4Ala;Thr10Ala;Thr46Ala;Thr60Ala;Thr101Ala;Thr104Ala] thr4ala, thr10ala, thr46ala, thr60ala, thr101ala, thr104ala mutation disrupting mutation disrupting interaction mutation disrupting alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain T A Fri Jul 22 11:08:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'thr104ala,thr10ala,thr60ala,thr101ala,thr46ala,thr4ala' Wed May 02 19:38:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr4ala,thr10ala,thr46ala,thr60ala,thr101ala,thr104ala' Tue Jun 30 16:52:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr4Ala;Thr10Ala;Thr46Ala;Thr60Ala;Thr101Ala;Thr104Ala]' schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 2 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association Figure 5A and 5B. Interaction between Pop2 and Cdc18 was detected in extracts from cells expressing HA-tagged pop2 and myc-tagged cdc18. This interaction is disrupted with mutant cdc18 indicating that pop2 only interacts with phosphorylated cdc18. To test for associations with specific target proteins, we examined the ability of Sud1 to interact physically with Cdc18. Through coprecipitation studies, we found that Sud1 and Cdc18 bind one another in vivo (Fig. 5A). To determine the functional significance of this interaction, we examined the role of Cdc18 phosphorylation in the process. As we reported previously, mutation of the six CDK consensus sites in Cdc18 abolishes its in vivo phosphorylation pattern and renders it immune to rapid degradation (6). Interestingly, the Cdc18DCDK1�6 mutant protein is incapable of binding to Sud1, unlike wild-type Cdc18 (Fig. 5B). These data indicate that Sud1 binding requires phosphorylation of Cdc18 by fission yeast CDKs and is required for rapid proteolysis of Cdc18. cdc18-pop2-2 1 3 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 2 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association Figure 5A. Interaction between Pop2 and Cdc18 was detected in extracts from cells expressing HA-tagged pop2 and myc-tagged cdc18. To test for associations with specific target proteins, we examined the ability of Sud1 to interact physically with Cdc18. Through coprecipitation studies, we found that Sud1 and Cdc18 bind one another in vivo (Fig. 5A).