UniProt UniProt http://www.uniprot.org Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae. Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae. Mol. Cell. Biol. 16 (3255-3263) 1996 Yashiroda H., Oguchi T., Yasuda Y., Toh-E A., Kikuchi Y. kikuchi@uts2.s.u-tokyo.ac.jp 01-April-2008: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid partial dna sequence hybrid partial dna sequence identification by hybridization partial dna sequence hybrid Yashiroda H., Oguchi T., Yasuda Y., Toh-E A., Kikuchi Y. kikuchi@uts2.s.u-tokyo.ac.jp Accepted 2008-MAR-22 by ORCHARD. 01-April-2008: Contacted by JYOTI. Mol. Cell. Biol. 16 (3255-3263) 1996 orchard Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae. Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae. Mol. Cell. Biol. 16 (3255-3263) 1996 Yashiroda H., Oguchi T., Yasuda Y., Toh-E A., Kikuchi Y. kikuchi@uts2.s.u-tokyo.ac.jp 01-April-2008: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yashiroda H., Oguchi T., Yasuda Y., Toh-E A., Kikuchi Y. kikuchi@uts2.s.u-tokyo.ac.jp Accepted 2008-MAR-22 by ORCHARD. 01-April-2008: Contacted by JYOTI. Mol. Cell. Biol. 16 (3255-3263) 1996 orchard Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae. Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae. Mol. Cell. Biol. 16 (3255-3263) 1996 Yashiroda H., Oguchi T., Yasuda Y., Toh-E A., Kikuchi Y. kikuchi@uts2.s.u-tokyo.ac.jp 01-April-2008: Contacted by JYOTI. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Yashiroda H., Oguchi T., Yasuda Y., Toh-E A., Kikuchi Y. kikuchi@uts2.s.u-tokyo.ac.jp Accepted 2008-MAR-22 by ORCHARD. 01-April-2008: Contacted by JYOTI. Mol. Cell. Biol. 16 (3255-3263) 1996 orchard bul1_yeast Ubiquitin ligase-binding protein BUL1 BUL1 DAG1 RDS1 YMR275C YM8021.01C YM8156.17C SMM2 Respiration deficiency suppressor 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAKDLNDSGFPPKRKPLLRPQRSDFTANSSTTMNVNANTRGRGRQKQEGGKGSSRSPSLHSPKSWIRSASATGILGLRRPELAHSHSHAPSTGTPAGGNRSPLRRSTANATPVETGRSLTDGDINNVVDVLPSFEMYNTLHRHIPQGNVDPDRHDFPPSYQEANNSTATGAAGSSADLSHQSLSTDALGATRSSSTSNLENLIPLRTEHHSIAAHQSTAVDEDSLDIPPILDDLNDTDNIFIDKLYTLPKMSTPIEITIKTTKHAPIPHVKPEEESILKEYTSGDLIHGFITIENKSQANLKFEMFYVTLESYISIIDKVKSKRTIKRFLRMVDLSASWSYSKIALGSGVDFIPADVDYDGSVFGLNNSRVLEPGVKYKKFFIFKLPLQLLDVTCKQEHFSHCLLPPSFGIDKYRNNCKYSGIKVNRVLGCGHLGTKGSPILTNDMSDDNLSINYTIDARIVGKDQKASKLYIMKEREYNLRVIPFGFDANVVGERTTMSQLNDITKLVQERLDALRKIFQRLEKKEPITNRDIHGADLSGTIDDSIESDSQEILQRKLDQLHIKNRNNYLVNYNDLKLGHDLDNGRSGNSGHNTDTSRAWGPFVESELKYKLKNKSNSSSFLNFSHFLNSSSSSMSSSSNAGKNNHDLTGNKERTGLILVKAKIPKQGLPYWAPSLLRKTNVFESKSKHDQENWVRLSELIPEDVKKPLEKLDLQLTCIESDNSLPHDPPEIQSITTELICITAKSDNSIPIKLNSELLMNKEKLTSIKALYDDFHSKICEYETKFNKNFLELNELYNMNRGDRRPKELKFTDFITSQLFNDIESICNLKVSVHNLSNIFKKQVSTLKQHSKHALSEDSISHTGNGSSSSPSSASLTPVTSSSKSSLFLPSGSSSTSLKFTDQIVHKWVRIAPLQYKRDINVNLEFNKDIKETLIPSFESCLCCRFYCVRVMIKFENHLGVAKIDIPISVRQVTK 9253D207B894CE44 9253D207B894CE44 rs5_yeast 40S ribosomal protein S5 RPS5 YJR123W J2045 RPS2 S2 YS8 RP14 Small ribosomal subunit protein uS7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDTEAPVEVQEDFEVVEEFTPVVLATPIPEEVQQAQTEIKLFNKWSFEEVEVKDASLVDYVQVRQPIFVAHTAGRYANKRFRKAQCPIIERLTNSLMMNGRNNGKKLKAVRIIKHTLDIINVLTDQNPIQVVVDAITNTGPREDTTRVGGGGAARRQAVDVSPLRRVNQAIALLTIGAREAAFRNIKTIAETLAEELINAAKGSSTSYAIKKKDELERVAKSNR CD3F743B56CB1127 bul1-rps5 3 5 unspecified role unspecified role neutral component neutral component n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 8 In order to prove that Bul1 and Rsp5 physically interact in vivo, we attempted to detect a complex of Bul1 and Rsp5 by centrifuging the cell lysates through a sucrose density gradient. bul1-rps5-1 2 5 unspecified role unspecified role prey prey n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 4 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 9 Rsp5 coimmunoprecipitates with Bul1. bul1-rps5-2 1 5 unspecified role unspecified role prey prey region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1641026]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 292-292, sequence length 225 [EBI-1641026]292-809 4 unspecified role unspecified role bait bait physical association physical association Fig. 1