IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Diverse cellular functions of the hsp90 molecular chaperone uncovered using systems approaches. Diverse cellular functions of the hsp90 molecular chaperone uncovered using systems approaches. Cell (0092-8674) 2007 imex curation McClellan AJ., Xia Y., Deutschbauer AM., Davis RW., Gerstein M., Frydman J. jfrydman@stanford.edu 13-Nov-2007: Contacted by JYOTI. davet Only protein-protein interactions 3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot McClellan AJ., Xia Y., Deutschbauer AM., Davis RW., Gerstein M., Frydman J. jfrydman@stanford.edu Accepted 2007-NOV-25 by DAVET. Cell (0092-8674) 2007 davet Only protein-protein interactions 3 imex curation cog8_yeast Conserved oligomeric Golgi complex subunit 8 YML071C COG8 DOR1 Component of oligomeric Golgi complex 8 protein protein yeast Saccharomyces cerevisiae Baker's yeast MELILNSLISDDLTEEQKRLSLDFLQDILQSNTKDYESYFSSRAVPGSITEDIAEIDAELSALDRKIRKTLLDNTSQIIGNILENDDRAQLDDIAKSLEQLWELDTNINKAADRNVTNDDINNESVSIDDFLEDDKEDNDTGRIMTTESNNLARKKKEDEFHKALSRLRNRISTKEDDKDDIRSDTLVTVLENLDSITDLMELPFLARTCIRTGHYQEAVMLYTHTTSLRSRFPGSTIVDEVCEKVLNEISTTMLSGLVKLLSTNVSVNSLKKILQYLNSIPPFDGKTNKSLLSVFLAMRYKFITDEIASYPLDVESSNESLIEMMVKRKIEVLREHVYMSLNVFLKSFLYDTNDLEIPFPEELESTVLRINGTNEEKEIEEKEKETKKEEYQKQDSVANNEEDVTENKSIEDVQEEVQGKVEGEDDGAERKTENEIENETVNKTEDKAEKEKEEEVNTKDNKAEKEEEEINKVEVTPEEPSKSIDNKAEKEEEEINKVEVTPEEPSKKIRTSKRENKIPTNAVMLQFVDKCITYVLKDLTRGLNSIKLSDSVCLQLVYCSFRLCDLNRNYHHLFLKKINDTSLFTTEQLARAIDKRAELASKYIYS 57E34E2DE36664B6 57E34E2DE36664B6 hsp82_yeast ATP-dependent molecular chaperone HSP82 HSP90 YPL240C HSP82 Heat shock protein Hsp90 heat-inducible isoform 82 kDa heat shock protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWESNAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLVVTKEVEKEVPIPEEEKKDEEKKDEEKKDEDDKKPKLEEVDEEEEKKPKTKKVKEEVQEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPFDLFESKKKKNNIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAEDSEQFEKFYSAFSKNIKLGVHEDTQNRAALAKLLRYNSTKSVDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITKDFELEETDEEKAEREKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMKAQALRDSSMSSYMSSKKTFEISPKSPIIKELKKRVDEGGAQDKTVKDLTKLLYETALLTSGFSLDEPTSFASRINRLISLGLNIDEDEETETAPEASTAAPVEEVPADTEMEEVD D7C35676D668FB63 D7C35676D668FB63 cog5_yeast Conserved oligomeric Golgi complex subunit 5 COG5 COD4 YNL051W N2476 YNL2476W Component of oligomeric Golgi complex 5 Complexed with DOR1 protein 4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTIAPMANDLEDFESLLEPDFDAKQFGNDLLKATNNNDTTILDLNTPLKKLNYDLHEIDSRIDQLMNSNPLEIIELIYKNEHVNSTIVGELKPSLGYMNMSYDRLKNQVLDPYERARKVQLALSKVYQTSFLLRGALLYIHLSNKLNALSKTAQLSTSTAINLASLHYQLEITLEENKNLKSLRKIKQLDQDIVSPNKRELITFLSLQMCKECLNSIKIKSNKEIISQLAYSLYLLSSQEFESAINKIVLSNVTMSSQILSKILNSIRMFPDAFNEVVEKGYNIYILETLLQNIKTDNVTNSSRSIAANKSRLGNLLSEYTSMKSKAGSGTPRDLFWSKVSSAFKKDFDISVNRGGPVGKSLLKNKDFIINTMKQSMKKSSDNSDYQSYLDVMLNSVSISLNK 619D6FF3B4A76A52 cog7_yeast Conserved oligomeric Golgi complex subunit 7 COG7 COD5 YGL005C Component of oligomeric Golgi complex 7 Complexed with DOR1 protein 5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVELTITGDDDDILSMFFDEEFVPHAFVDILLSNALNEDQIQTQSVSSLLLTRLDFYTKNLTKELESTIWNLDKLSQTLPRTWASSRYHKEAEQNDSSLYSTESLKSSKLEYYLDTLASAVRALETGMHNVTEKLSDLDNENNRNTNVRQQLQSLMLIKERIEKVVYYLEQVRTVTNISTVRENNTTSTGTDLSITDFRTSLKALEDTIDESLSSAIDNEAKDETNKDLIGRIDSLSELKCLFKGLDKFFAEYSNFSESIKSKAQSYLSTKNIDDGMIS A72B39889DC74625 cog5-hsp90-1 1 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey physical association physical association Fig. 6C Affinity isolation of GST-tagged COG subunits followed by immunoblot analysis for Hsp90 demonstrated that Hsp90 physically interacts with COG complex lobe B subunits Cog5p, Cog7p, and Cog8p cog7-hsp90-1 1 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 6C Affinity isolation of GST-tagged COG subunits followed by immunoblot analysis for Hsp90 demonstrated that Hsp90 physically interacts with COG complex lobe B subunits Cog5p, Cog7p, and Cog8p cog8-hsp90-1 1 2 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey physical association physical association Fig. 6C Affinity isolation of GST-tagged COG subunits followed by immunoblot analysis for Hsp90 demonstrated that Hsp90 physically interacts with COG complex lobe B subunits Cog5p, Cog7p, and Cog8p