IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ PKA-I holoenzyme structure reveals a mechanism for cAMP-dependent activation. PKA-I holoenzyme structure reveals a mechanism for cAMP-dependent activation. Cell (0092-8674) 2007 imex curation Kim C., Cheng CY., Saldanha SA., Taylor SS. staylor@ucsd.edu 01-Feb-2008: Contacted by JYOTI. DAVET Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kim C., Cheng CY., Saldanha SA., Taylor SS. staylor@ucsd.edu Accepted 2008-FEB-9 by DAVET. 01-Feb-2008: Contacted by JYOTI. Cell (0092-8674) 2007 DAVET Only protein-protein interactions imex curation amp-pnp AMP-PNP small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) kapca_mouse cAMP-dependent protein kinase catalytic subunit alpha Prkaca Pkaca protein protein mouse Mus musculus Mouse MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF DIP protein P05132 original sequence version: 185 DIP protein P05132 original sequence version: 186 DIP protein P05132-1 original sequence version: 145 DIP protein P05132-1 original sequence version: 193 DIP protein P05132-1 original sequence version: 199 DIP protein P05132 original sequence version: 163 DIP protein P05132 original sequence version: 192 DIP protein P05132-1 original sequence version: 197 DIP protein P05132-1 original sequence version: 149 02F85D66EB21A1FA kap0_bovin cAMP-dependent protein kinase type I-alpha regulatory subunit PRKAR1A protein protein bovin Bos taurus (Bovine) Bovine MASGTTASEEERSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFEKLEKEEAKQIQNLQKAGSRADSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFPVSFIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV DIP protein P00514 original sequence version: 101 DIP protein P00514 original sequence version: 111 DIP protein P00514 original sequence version: 133 DIP protein P00514 original sequence version: 149 B086A291809422F4 manganese(2+) Manganese (2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) prkaca-prkar1a-3 1 2 unspecified role unspecified role neutral component neutral component 5 cofactor cofactor coenzyme neutral component neutral component 4 unspecified role unspecified role neutral component neutral component asp-268 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain P00514:p.Arg334Lys mutation mutation certain certain sequence position certain certain certain sequence position certain R K Wed May 02 16:54:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg334lys' Tue Jun 30 19:23:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg334Lys' ecobb Escherichia coli (strain B / BL21) 3 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1554635]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 379-379, sequence length 351 [EBI-1554635]91-379 arg-195 binding region binding-associated region binding region x-ray diffraction x-ray crystallography X-ray x-ray diffraction X-ray certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 10 14 direct interaction direct interaction 2.2A We report here the structure of a complex between the PKA catalytic subunit and a mutant RI regulatory subunit, alpha(91-379:R333K), containing both cAMP-binding domains. Residue numbering in the paper uses the cleaved initiator methionine as position 0 0.192