IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Arabidopsis CBF5 interacts with the H/ACA snoRNP assembly factor NAF1. Arabidopsis CBF5 interacts with the H/ACA snoRNP assembly factor NAF1. Plant Mol. Biol. (0167-4412) 2007 imex curation Lermontova I., Schubert V., Bornke F., Macas J., Schubert I. lermonto@ipk-gatersleben.de. 12-Oct-2007: Contacted by JYOTI. Arabidopsis inflorescence cDNA library orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lermontova I., Schubert V., Bornke F., Macas J., Schubert I. lermonto@ipk-gatersleben.de. Accepted 2007-OCT-21 by ORCHARD. Arabidopsis inflorescence cDNA library Plant Mol. Biol. (0167-4412) 2007 orchard Only protein-protein interactions imex curation Arabidopsis CBF5 interacts with the H/ACA snoRNP assembly factor NAF1. Arabidopsis CBF5 interacts with the H/ACA snoRNP assembly factor NAF1. Plant Mol. Biol. (0167-4412) 2007 imex curation Lermontova I., Schubert V., Bornke F., Macas J., Schubert I. lermonto@ipk-gatersleben.de. 12-Oct-2007: Contacted by JYOTI. Arabidopsis inflorescence cDNA library orchard Only protein-protein interactions tobac-leaf Nicotiana tabacum (tobacco) leaf leaf leaf bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Lermontova I., Schubert V., Bornke F., Macas J., Schubert I. BiFC assays were performed in Agrobacterium-infiltrated tobacco (Nicotiana benthamiana) leaves. lermonto@ipk-gatersleben.de. Accepted 2007-OCT-21 by ORCHARD. 12-Oct-2007: Contacted by JYOTI. Arabidopsis inflorescence cDNA library Plant Mol. Biol. (0167-4412) 2007 orchard Only protein-protein interactions imex curation cbf5_arath H/ACA ribonucleoprotein complex subunit 4 CBF5 CBF5 homolog Dyskerin NAP57 Nopp-140-associated protein of 57 kDa homolog Nucleolar protein NAP57 homolog F24I3.230 At3g57150 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAEVDISHSKKKKQDKTENDAADTGDYMIKPQSFTPAIDTSQWPILLKNYDRLNVRTGHYTPISAGHSPLKRPLQEYIRYGVINLDKPANPSSHEVVAWIKRILRVEKTGHSGTLDPKVTGNLIVCIDRATRLVKSQQGAGKEYVCVARLHSAVPDVAKVARALESLTGAVFQRPPLISAVKRQLRIRTIYESKLLEYDADRHLVVFWVSCEAGTYIRTMCVHLGLLLGVGGHMQELRRVRSGILGENNNMVTMHDVMDAQFVYDNSRDESYLRRVIMPLEMILTSYKRLVVKDSAVNAICYGAKLMIPGLLRFENDIDVGTEVVLMTTKGEAIAVGIAEMTTSVMATCDHGVVAKIKRVVMDRDTYPRKWGLGPRASMKKKLIADGKLDKHGKPNEKTPVEWSRNVVLPTGGDAIIAGAAAAPEEIKADAENGEAGEARKRKHDDSSDSPAPVTTKKSKTKEVEGEEAEEKVKSSKKKKKKDKEEEKEEEAGSEKKEKKKKKDKKEEVIEEVASPKSEKKKKKKSKDTEAAVDAEDESAAEKSEKKKKKKDKKKKNKDSEDDEE DE731AA935277D54 q84tg5_arath H/ACA ribonucleoprotein complex non-core subunit NAF1 At1g03530 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVGFPANQAKTVEEELPLQVVADQDPEVKTFKDSFDFPSIDSFPDFDSITWSESNRNVEDFSIEDTDFDFFEYHMEIPQEDVVTVSDVMCPENQTTVNSESIEGKLEVFNDDMSGEVGVSSVRSESMVEVKPVLCDVMSEEVGVSSVEVEPNVCVEMSANGGDEPAVKDSEPVVSENSRSMEGETEPVVVSDASVACPTMDLDESGLKKTDEGLACSIEVGLEKVSLAVDDDEKSDEAKGEMDSAESESETSSSSASSSDSSSSEEEESDEDESDKEENKKEEKFEHMVVGKEDDLAGELEEGEIENLDEENGDDDIEDEDDDDDDDDDDDDDVNEMVAWSNDEDDDLGLQTKEPIRSKNELKELPPVPPVDVTLEPHHATLPVGVVLSVMSAQVIVEGMEKHSPLTEGSILWITEKRTPLGLVDEIFGPVKCPYYIVRFNSESEVPEGVCQGTPVSFVADFAQHILNIKELQKKGYDASGDNDEEIPDELEFSDDEKEAEYRRMQKLEKRGMMSDQKTGNTRNKKKKNRDPGRPTSSYSGEWTKNQGSSSLSSNRSDPQMGGPRPQMDGFPPNNAAWRPQSNQQNPYQLPPIPNQMGMQIPFMAMQNQNQMMFQPQFNGGQMPMPGGPGGLNFFPGQASAPWPAMVGQNCFNQQFGMGRGIQQQPLPNELSFNMFSQGLQMHPPQSQMHRPQSQMNPQFQMPPQFQPHQQSPMNPQYQMMHRPQSPANPQFQMQAQSDVRPLQSQIPQSPSDLQSPMEPQSQGFSSGQSSERGRGFHGRGRGRGRGRFGRGRGRGRQQSG C4B1AC645D03E983 cbf5-at1g03530 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association Sequencing the cDNA inserts of eight randomly chosen potential CBF5 interaction partners and subsequent database searches revealed that they all matched to At1g03530, a previously uncharacterized protein of 801aa and a predicted molecular weight of 88.8 kD. cbf5-at1g03530-1 2 3 unspecified role unspecified role neutral component neutral component region n-terminal region of the yfp protein yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role neutral component neutral component region c-terminal region of the yfp protein yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association BiFC analysis showing interaction of AtCBF5-NYFP and AtNAF1-CYFP within nuclei of transiently transformed N. benthamiana leaves 5 The authors report that colocalisation was observed in nucleoplasm, nuclear bodies and nucleolus.