IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ IKK beta suppression of TSC1 links inflammation and tumor angiogenesis via the mTOR pathway. IKK beta suppression of TSC1 links inflammation and tumor angiogenesis via the mTOR pathway. Cell (0092-8674) 2007 imex curation Lee DF., Kuo HP., Chen CT., Hsu JM., Chou CK., Wei Y., Sun HL., Li LY., Ping B., Huang WC., He X., Hung JY., Lai CC., Ding Q., Su JL., Yang JY., Sahin AA., Hortobagyi GN., Tsai FJ., Tsai CH., Hung MC. Cancer - Interactions investigated in the context of cancer Source organism from which TSC1 was cloned supplied by author, details of IKKbeta taken from manufacturer's website. mhung@mdanderson.org 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions 4 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lee DF., Kuo HP., Chen CT., Hsu JM., Chou CK., Wei Y., Sun HL., Li LY., Ping B., Huang WC., He X., Hung JY., Lai CC., Ding Q., Su JL., Yang JY., Sahin AA., Hortobagyi GN., Tsai FJ., Tsai CH., Hung MC. Cancer - Interactions investigated in the context of cancer Source organism from which TSC1 was cloned supplied by author, details of IKKbeta taken from manufacturer's website. mhung@mdanderson.org Accepted 2007-OCT-25 by JYOTI. 12-Oct-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation IKK beta suppression of TSC1 links inflammation and tumor angiogenesis via the mTOR pathway. IKK beta suppression of TSC1 links inflammation and tumor angiogenesis via the mTOR pathway. Cell (0092-8674) 2007 imex curation Lee DF., Kuo HP., Chen CT., Hsu JM., Chou CK., Wei Y., Sun HL., Li LY., Ping B., Huang WC., He X., Hung JY., Lai CC., Ding Q., Su JL., Yang JY., Sahin AA., Hortobagyi GN., Tsai FJ., Tsai CH., Hung MC. Cancer - Interactions investigated in the context of cancer Source organism from which TSC1 was cloned supplied by author, details of IKKbeta taken from manufacturer's website. mhung@mdanderson.org 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions 4 human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lee DF., Kuo HP., Chen CT., Hsu JM., Chou CK., Wei Y., Sun HL., Li LY., Ping B., Huang WC., He X., Hung JY., Lai CC., Ding Q., Su JL., Yang JY., Sahin AA., Hortobagyi GN., Tsai FJ., Tsai CH., Hung MC. Cancer - Interactions investigated in the context of cancer Source organism from which TSC1 was cloned supplied by author, details of IKKbeta taken from manufacturer's website. mhung@mdanderson.org Accepted 2007-OCT-25 by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation IKK beta suppression of TSC1 links inflammation and tumor angiogenesis via the mTOR pathway. IKK beta suppression of TSC1 links inflammation and tumor angiogenesis via the mTOR pathway. Cell (0092-8674) 2007 imex curation Lee DF., Kuo HP., Chen CT., Hsu JM., Chou CK., Wei Y., Sun HL., Li LY., Ping B., Huang WC., He X., Hung JY., Lai CC., Ding Q., Su JL., Yang JY., Sahin AA., Hortobagyi GN., Tsai FJ., Tsai CH., Hung MC. Cancer - Interactions investigated in the context of cancer Source organism from which TSC1 was cloned supplied by author, details of IKKbeta taken from manufacturer's website. mhung@mdanderson.org 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions 4 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Lee DF., Kuo HP., Chen CT., Hsu JM., Chou CK., Wei Y., Sun HL., Li LY., Ping B., Huang WC., He X., Hung JY., Lai CC., Ding Q., Su JL., Yang JY., Sahin AA., Hortobagyi GN., Tsai FJ., Tsai CH., Hung MC. Cancer - Interactions investigated in the context of cancer Source organism from which TSC1 was cloned supplied by author, details of IKKbeta taken from manufacturer's website. mhung@mdanderson.org Accepted 2007-OCT-25 by JYOTI. 12-Oct-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation tsc2_human Tuberin TSC2 Tuberous sclerosis 2 protein TSC4 protein protein human Homo sapiens Human MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV 7B915C46970D7D31 tsc1_human Hamartin TSC1 KIAA0243 Tuberous sclerosis 1 protein TSC protein protein human Homo sapiens Human MAQQANVGELLAMLDSPMLGVRDDVTAVFKENLNSDRGPMLVNTLVDYYLETSSQPALHILTTLQEPHDKHLLDRINEYVGKAATRLSILSLLGHVIRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVAEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTTSPYADTQNSYGCATSTPYSTSRLMLLNMPGQLPQTLSSPSTRLITEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPLCHSDDYVHISLPQATVTPPRKEERMDSARPCLHRQHHLLNDRGSEEPPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISRELSEITTAEAEPVVPRGGFDSPFYRDSLPGSQRKTHSAASSSQGASVNPEPLHSSLDKLGPDTPKQAFTPIDLPCGSADESPAGDRECQTSLETSIFTPSPCKIPPPTRVGFGSGQPPPYDHLFEVALPKTAHHFVIRKTEELLKKAKGNTEEDGVPSTSPMEVLDRLIQQGADAHSKELNKLPLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYNQLQEQRDTMVTKLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQTQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLDCCNDGCSDSMVGHNEEASGHNGETKTPRPSSARGSSGSRGGGGSSSSSSELSTPEKPPHQRAGPFSSRWETTMGEASASIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDGMTSSLSESLKTELGKDLGVEAKIPLNLDGPHPSPPTPDSVGQLHIMDYNETHHEHS EF15509385C7AACC ikkb_human Inhibitor of nuclear factor kappa-B kinase subunit beta IKBKB IKKB I-kappa-B kinase 2 Nuclear factor NF-kappa-B inhibitor kinase beta Serine/threonine protein kinase IKBKB protein protein human Homo sapiens Human MSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQAS DIP protein O14920 original sequence version: 89 DIP protein O14920 original sequence version: 100 DIP protein O14920 original sequence version: 121 DIP protein O14920 original sequence version: 126 DIP protein O14920 original sequence version: 136 DIP protein O14920 original sequence version: 128 DIP protein O14920-1 original sequence version: 151 DIP protein O14920 original sequence version: 142 DIP protein O14920 original sequence version: 159 DIP protein O14920 original sequence version: 93 DIP protein O14920 original sequence version: 124 DIP protein O14920-1 original sequence version: 178 DIP protein O14920 original sequence version: 85 F9CADF671AE9E14E tsc1-ikkb-1 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association This interaction between IKKbeta and TSC1 was also observed with endogenous IKKbeta and TSC1 Fig 4D tsc1-ikkb-2 3 6 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 5 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction In vitro kinase assays demonstrated that the TSC1 domain 301-600 was strongly phosphorylated by IKKβ, whereas other domains of TSC1 were not phosphorylated by IKKβ under the same conditions (Figure S7A, lanes 1-4). Fig. 4F and Supp Fig. 7A, Ser-487 and Ser-511 of TSC1 is phosphorylated by IKKbeta Ser-487 and Ser-511 mutations to alanine abolished phosphorylation of TSC1 by IKKbeta tsc1-ikkb-4 1 6 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. 5 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction In vitro transcribed TSC1 proteins were incubated with recombinant IKKbeta proteins and then pulled down by antibody to IKKbeta. Fig 4E tsc1-tsc2-1 2 5 unspecified role unspecified role prey prey Q92574:p.[Ser487Asp;Ser511Asp] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S D certain certain sequence position certain certain certain sequence position certain S D Fri Jul 22 11:15:42 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser511asp,ser487asp' Wed May 02 16:48:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser487asp,ser511asp' Tue Jun 30 18:42:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser487Asp;Ser511Asp]' 4 unspecified role unspecified role bait bait physical association physical association Fig. 5E and Sup 10D Indeed, TNFalpha treatment, which induced IKKbeta to phosphorylate TSC1, interfered with TSC complex formation (Figure 5E). TNF-alpha