IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Functional similarity between the chloroplast translocon component, Tic40, and the human co-chaperone, Hsp70-interacting protein (Hip). Functional similarity between the chloroplast translocon component, Tic40, and the human co-chaperone, Hsp70-interacting protein (Hip). J. Biol. Chem. (0021-9258) 2007 imex curation Bedard J., Kubis S., Bimanadham S., Jarvis P. Apoptosis - Interactions involving proteins with a function related to apoptosis rpj3@le.ac.uk 12-Oct-2007: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bedard J., Kubis S., Bimanadham S., Jarvis P. Apoptosis - Interactions involving proteins with a function related to apoptosis rpj3@le.ac.uk Accepted 2007-OCT-10 by ORCHARD. J. Biol. Chem. (0021-9258) 2007 orchard Only protein-protein interactions imex curation Functional similarity between the chloroplast translocon component, Tic40, and the human co-chaperone, Hsp70-interacting protein (Hip). Functional similarity between the chloroplast translocon component, Tic40, and the human co-chaperone, Hsp70-interacting protein (Hip). J. Biol. Chem. (0021-9258) 2007 imex curation Bedard J., Kubis S., Bimanadham S., Jarvis P. Apoptosis - Interactions involving proteins with a function related to apoptosis rpj3@le.ac.uk 12-Oct-2007: Contacted by JYOTI. orchard Only protein-protein interactions arath-leaf Arabidopsis thaliana leaf leaf leaf bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Bedard J., Kubis S., Bimanadham S., Jarvis P. Apoptosis - Interactions involving proteins with a function related to apoptosis rpj3@le.ac.uk Accepted 2007-OCT-10 by ORCHARD. 12-Oct-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2007 orchard Only protein-protein interactions imex curation lt_sv40 Large T antigen protein protein sv40 sv40 SV40 MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKKPPTPPPEPET 3E37D4BAFC5D59BD ti110_arath Protein TIC110, chloroplastic TIC110 Translocon at the inner envelope membrane of chloroplasts 110 F4H5.1 F10K1.33 At1g06950 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNPSLVTAINAPISPSPRSPLLSHFLPTLPHRFSKSECLSRRRYRVSFPRSSAASSDQLSVSTQAKNPGIHGNKKELTGLQPIVEKMTPPVRLATSAVVLAASLATGYGLGLRLAGSRNIAFGGAAVAGAAGGAVVYALNSAVPEVAAISLHNYVAEFEDPASVTKDDVEKIADRYGVNKGDEAFQAEICDIYCRYVTSVLPTEGQSLKGDEVAKIVKFKNALGIDEPDAAAMHMEIGRRIFRQRLETGEREGDAEQRRAFMRLVYVSALVFGDASSFLLPWKRVLKVTDAQVEIAIRENAKQLYAERLKLVGRDINVENLVDLRKSQLSFKLSDELAEDLFREHTRKVVVENISSALSILKSRTRAAKSLASVVEELEKVLEFNNLLVSLKSHSEADQFARGVGPISLIGDESDFERRMDDLKLLYRAYVTDALSGGRLEENKLVAMSQLRNILGLGKREAEAISVDVTSKSYRKRLANAVSSGDLEAQDSKAKYLQKLCEELHFDAQKAGAIHEEIYRQKLQQCVTDGELSDDNVAALLRLRVMLCIPQQTVDTAHAEICGTIFEKVVRDAISSGVDGYDAETRKSVRKAAHGLRLSRETAMSIASKAVRRVFTNYIRRARAAENRTDSAKELKKMIAFNTLVVTEMVADIKGESSDKAPEEDPVQEKEEDDEDEEWGSLESLRKTRPDKELAEKMGKPGQTEITLKDDLPDRDRIDLYKTYLLYCVTGEVTRIPFGAQITTKRDDSEYLLLNQLGGILGLSSKEIVNIHVGLAEQAFRQQAEVILADGQLTKARVEQLDELQKQVGLPQPQAEKVIKNITTTKMANAIETAVNQGRLNIKQIRELKEANVSLDSMIAVSLREKLFKKTVSDIFSSGTGEFDETEVYQTIPSDLSIDVEKAKRVVHDLAQSRLSNSLVQAVALLRQRNSKGVVLSLNDLLACDKAVPAEPMSWEVSEELSDLYAIYSKSDPKPAPEKVLRLQYLLGIDDSTATALREMEDGALSSAAEEGNFVF 814CCDE07EB5D0EA ftz21_arath Cell division protein FtsZ homolog 2-1, chloroplastic FTSZ2-1 At2g36250 Plastid division protein FTSZ2-1 F2H17.14 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATYVSPCFTPSDSRLLTVLRKNVLPENHLGRLNSIRTIDSKKNRVVVAAQKSESSPIRNSPRHYQSQAQDPFLNLHPEISMLRGEGTSTIVNPRKETSSGPVVEDFEEPSAPSNYNEARIKVIGVGGGGSNAVNRMIESEMSGVEFWIVNTDIQAMRMSPVLPDNRLQIGKELTRGLGAGGNPEIGMNAARESKEVIEEALYGSDMVFVTAGMGGGTGTGAAPVIAGIAKAMGILTVGIATTPFSFEGRRRTVQAQEGLASLRDNVDTLIVIPNDKLLTAVSQSTPVTEAFNLADDILRQGVRGISDIITIPGLVNVDFADVRAIMANAGSSLMGIGTATGKSRARDAALNAIQSPLLDIGIERATGIVWNITGGSDLTLFEVNAAAEVIYDLVDPTANLIFGAVVDPALSGQVSITLIATGFKRQEEGEGRTVQMVQADAASVGATRRPSSSFRESGSVEIPEFLKKKGSSRYPRV A5CE7F111C962044 p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD DIP protein P02340 original sequence version: 117 DIP protein P02340 original sequence version: 165 DIP protein P02340 original sequence version: 187 DIP protein P02340 original sequence version: 201 DIP protein P02340 original sequence version: 111 DIP protein P02340 original sequence version: 141 DIP protein P02340 original sequence version: 180 DIP protein P02340 original sequence version: 163 DIP protein P02340 original sequence version: 152 ED5A607378D921D7 tic40_arath Protein TIC 40, chloroplastic TIC40 PDE120 At5g16620 Protein PIGMENT DEFECTIVE EMBRYO 120 Translocon at the inner envelope membrane of chloroplasts 40 MTG13.6 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MENLTLVSCSASSPKLLIGCNFTSSLKNPTGFSRRTPNIVLRCSKISASAQSQSPSSRPENTGEIVVVKQRSKAFASIFSSSRDQQTTSVASPSVPVPPPSSSTIGSPLFWIGVGVGLSALFSYVTSNLKKYAMQTAMKTMMNQMNTQNSQFNNSGFPSGSPFPFPFPPQTSPASSPFQSQSQSSGATVDVTATKVETPPSTKPKPTPAKDIEVDKPSVVLEASKEKKEEKNYAFEDISPEETTKESPFSNYAEVSETNSPKETRLFEDVLQNGAGPANGATASEVFQSLGGGKGGPGLSVEALEKMMEDPTVQKMVYPYLPEEMRNPETFKWMLKNPQYRQQLQDMLNNMSGSGEWDKRMTDTLKNFDLNSPEVKQQFNQIGLTPEEVISKIMENPDVAMAFQNPRVQAALMECSENPMNIMKYQNDKEVMDVFNKISQLFPGMTG 52D3E7222AB461CE tic40-tic110 1 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 10 physical association physical association 6A To address this possibility, we subjected atTic110 and atTic40 to yeast two-hybrid interaction analysis. As shown in Fig. 6 (A and B), the results provided clear evidence of a significant, if rather weak, interaction between the proteins. tic40-tic110-1 2 7 unspecified role unspecified role neutral component neutral component region c-terminal region of the yfp protein tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role neutral component neutral component n-terminal n-terminal region of the yfp protein tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 6C These data nicely corroborate previous findings based on biochemical analysis (which showed that Tic110 and Tic40 are envelope proteins) and indicated that BiFC fluorescence associated with an atTic110-atTic40 interaction should localize to the envelope. sv40-tp53-2 1 6 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Vigorous growth of cells carrying the pGBD-p53 plus pGAD-SV40T control plasmids indicated a very strong interaction between murine p53 and SV40 large T-antigen, as expected. 6 ftsz2_1-ftsz2_1 2 5 unspecified role unspecified role neutral component neutral component region c-terminal region of the yfp protein tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component region n-terminal region of the yfp protein tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S5 The Tic110-Tic40 BiFC signal was also stronger than that mediated by AtFtsZ2-1 homodimerization