MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Mus81 cleavage of Holliday junctions: a failsafe for processing meiotic recombination intermediates? Mus81 cleavage of Holliday junctions: a failsafe for processing meiotic recombination intermediates? EMBO J. (0261-4189) 2007 imex curation Gaskell LJ., Osman F., Gilbert RJ., Whitby MC. 2009-01-08 Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Gaskell LJ., Osman F., Gilbert RJ., Whitby MC. EMBO J. (0261-4189) 2007 2009-01-08 Only protein-protein interactions imex curation mus81_schpo Crossover junction endonuclease mus81 mus81 SPCC4G3.05c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDCGNPLFLQWIQEWMEESTRRFPKSYQTWRKAYDSMKSCPITFHRPSQALALKGIGPTICAKLEKKWNAYCLENNIPISTHNEQNDSHVNANKSSSETSSEKPRSVKKPTTRKRKVYVPSYRSGAYSILCALYMLNKHEFATKPQIVTMAQPYCDSSFGSATDRNMRYTAWSAMKTLITKNLVYQTGHPSKYCLTDDGEEVCIRLAKVDDSFQRKHTVSNFSVSKSDDHDSSLCQPPNFVTSINKAGSSSDHGGELHVTYCPVDHNEVSDGVETDIDVDQVDSLTGIHDHHIINNEQLIDLTEQEKKQPNESNLSNLKIETVLFSNCTVFLLIDTREIRSPLDRNLIIDKLTNDFGVNCQVRSLELGDALWVARDMESGQEVVLDFVVERKRYDDLVASIKDGRFHEQKARLKKSGIRSVTYILEESSYDESFTESIRTAVSNTQVDQLFHVRHTRSLEHSVSLLAEMTKQINLFYEKRKTLAVIPDLSIEAKTYESLREQLLKIDPSTPYHISYHAFSSVLSKSSTLTVGDIFIRMLMTIKGISASKAIEIQKKYPTFMHLFEAYEKSSSSQERNLLLNKTCQGYGFQTIGPALSAKVASVFFPES mint Interacts with eme1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI) 481EBE3846931732 mms4_yeast Crossover junction endonuclease MMS4 MMS4 SLX2 YBR0826 YBR0829 YBR100W YBR098W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQIVDFVEDKDSRNDASIQIIDGPSNVEIIALSESMDQDECKRAHVSSAEMIPSSPQRKSVSNDVENVDLNKSIELSAPFFQDISISKLDDFSTTVNSIIDSSLRNENNAKGNAKKLLDDLISDEWSADLESSGKKHNKSQYNLRDIAEKWGVQSLKNPEPIAVDCEYKTQGIGKTNSDISDSPKSQIGAADILFDFPLSPVKHENPTEEKHNSIANENSSPDNSLKPAGKQNHGEDGTSMAKRVYNKGEDEQEHLPKGKKRTIALSRTLINSTKLPDTVELNLSKFLDSSDSITTDVLSTPAKGSNIVRTGSQPIFSNANCFQEAKRSKTLTAEDPKCTKNTAREVSQLENYIAYGQYYTREDSKNKIRHLLKENKNAFKRVNQIYRDNIKARSQMIIEFSPSLLQLFKKGDSDLQQQLAPAVVQSSYNDSMPLLRFLRKCDSIYDFSNDFYYPCDPKIVEENVLILYYDAQEFFEQYTSQKKELYRKIRFFSKNGKHVILILSDINKLKRAIFQLENEKYKARVEQRLSGTEEALRPRSKKSSQVGKLGIKKFDLEQRLRFIDREWHVKIHTVNSHMEFINSLPNLVSLIGKQRMDPAIRYMKYAHLNVKSAQDSTETLKKTFHQIGRMPEMKANNVVSLYPSFQSLLEDIEKGRLQSDNEGKYLMTEAVEKRLYKLFTCTDPNDTIE 3B3918DD3B2E7E0C 3B3918DD3B2E7E0C mus81_yeast Crossover junction endonuclease MUS81 MUS81 SLX3 YDR386W D9509.6 MMS and UV-sensitive protein 81 protein protein yeast Saccharomyces cerevisiae Baker's yeast MELSSNLKDLYIEWLQELVDGLTPKQEQLKIAYEKAKRNLQNAEGSFYYPTDLKKVKGIGNTIIKRLDTKLRNYCKIHHISPVEAPSLTQTSSTRPPKRTTTALRSIVNSCENDKNEAPEEKGTKKRKTRKYIPKKRSGGYAILLSLLELNAIPRGVSKEQIIEVAGKYSDHCMTPNFSTKEFYGAWSSIAALKKHSLVLEEGRPKRYSLTEEGVELTKSLKTADGISFPKENEEPNEYSVTRNESSEFTANLTDLRGEYGKEEEPCDINNTSFMLDITFQDLSTPQRLQNNVFKNDRLNSQTNISSHKLEEVSDDQTVPDSALKAKSTIKRRRYNGVSYELWCSGDFEVFPIIDHREIKSQSDREFFSRAFERKGMKSEIRQLALGDIIWVAKNKNTGLQCVLNTIVERKRLDDLALSIRDNRFMEQKNRLEKSGCEHKYYLIEETMSGNIGNMNEALKTALWVILVYYKFSMIRTCNSDETVEKIHALHTVISHHYSQKDLIVIFPSDLKSKDDYKKVLLQFRREFERKGGIECCHNLECFQELMGKGDLKTVGELTIHVLMLVKGISLEKAVAIQEIFPTLNKILMAYKTCSSEEEAKLLMFNVLGDAPGAKKITKSLSEKIYDAFGKL F73FA3856C5F87F1 F73FA3856C5F87F1 eme1_schpo Crossover junction endonuclease eme1 eme1 mms4 slx2 SPAPB1E7.06c Essential meiotic endonuclease 1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTLQSTDSAIVIDSEADVDISSSQASPSKDNIALSEHNVITVLDTPQRSTQCDSLLKSFSTPLVSGSEDVLPSPRDALNITNKKSVTDNLLLSLTSSNQSTNTNLNPSSRVEIINLNSSPPNSLSSQPKHQEFHLFHTPTIPRTTQLSSKTSSPIVIPDDNEQVASPLSKKAASLTSSPLKDFQSSPPLSTVLQKSHSLHDILLDTNDDDHFPFTQSPLTKTKSFNDALTSSSSILKPCMPSIASPTSNRLSHAPSTPNLFPNQDSSNTIDLINNRSKTSVENQERTFNLTSDVHLDSPTSPSKHSSIEPNTSQEDSFQELPSLNKLSIQSRAFKKMRLPKISRTTDTPPASTSNSNKKNLDKLKKMRKLCSRSLEPYELDSNTQRKRKRYEDSLKKSKTLDKVDSLNRKMAKELDRKNSKELQKINKVKRTKEECLSEIILLSPDDWASSWYSTVRSQLDSYNCQFVVNSNQPKDSIMWKRKVNNVFNSSTNRFELSIEHEQIEPFALLRLKCRDFIKYIEEDQADTFFHEMSEKFKGCKLILLLEGIPNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRFSFEHSILINTSNDEKETAQWIVSFTGDIALSRYKHFSKFSARASTTEIGHVKSADRIENSLNFMLRQILRVTPNIANAICDQFDSIPSLIHHLKTHGEESLTNVVIQSSISERNLGPVLSRRIYNTFLCKEASSDAP mint Interacts with mus81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI) 275825D6875A9838 eme1-mus81-2 1 5 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 2 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction F2 EMBO-Journal mint mms4-mus81 1 3 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 predetermined predetermined participant predetermined predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction SF2 EMBO-Journal mint