IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. J. Mol. Biol. (0022-2836) 2007 imex curation Di Fulvio M., Frondorf K., Henkels KM., Lehman N., Gomez-Cambronero J. julian.cambronero@wright.edu 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA orchard Only protein-protein interactions human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Di Fulvio M., Frondorf K., Henkels KM., Lehman N., Gomez-Cambronero J. PLD activity is not entered since whole cell lysates used to check activity and we cannot be sure that PLD2 is the enzyme. julian.cambronero@wright.edu Accepted 2007-SEP-10 by ORCHARD. 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA J. Mol. Biol. (0022-2836) 2007 orchard Only protein-protein interactions imex curation The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. J. Mol. Biol. (0022-2836) 2007 imex curation Di Fulvio M., Frondorf K., Henkels KM., Lehman N., Gomez-Cambronero J. julian.cambronero@wright.edu 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA orchard Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Di Fulvio M., Frondorf K., Henkels KM., Lehman N., Gomez-Cambronero J. julian.cambronero@wright.edu Accepted 2007-SEP-10 by ORCHARD. 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA J. Mol. Biol. (0022-2836) 2007 orchard Only protein-protein interactions imex curation The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. J. Mol. Biol. (0022-2836) 2007 imex curation Di Fulvio M., Frondorf K., Henkels KM., Lehman N., Gomez-Cambronero J. julian.cambronero@wright.edu 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA orchard Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Di Fulvio M., Frondorf K., Henkels KM., Lehman N., Gomez-Cambronero J. julian.cambronero@wright.edu Accepted 2007-SEP-10 by ORCHARD. 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA J. Mol. Biol. (0022-2836) 2007 orchard Only protein-protein interactions imex curation The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. J. Mol. Biol. (0022-2836) 2007 imex curation Di Fulvio M., Frondorf K., Henkels KM., Lehman N., Gomez-Cambronero J. julian.cambronero@wright.edu 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA orchard Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Di Fulvio M., Frondorf K., Henkels KM., Lehman N., Gomez-Cambronero J. julian.cambronero@wright.edu Accepted 2007-SEP-10 by ORCHARD. 07-Sept-2007: Contacted by JYOTI. 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA J. Mol. Biol. (0022-2836) 2007 orchard Only protein-protein interactions imex curation grb2_cerae_protein Cercopithecus aethiops Grb2 protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt Similar to P62993 pld2_human Phospholipase D2 Choline phosphatase 2 Phosphatidylcholine-hydrolyzing phospholipase D2 PLD1C PLD2 protein protein human Homo sapiens Human MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQSLKVHPLVFAPGVPVTAQVVGTERYTSGSKVGTCTLYSVRLTHGDFSWTTKKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRAGPEGSTRHAASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGRKGLEGMIRKRSGGHRVPGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCLETGAISFVQLFDPGFEVQVGKRSTEARHGVRIDTSHRSLILKCSSYRQARWWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVADAILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGRFALSLRKHCFGVILGANTRPDLDLRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT DIP protein O14939 original sequence version: 128 F25F6B73B45F57ED sos_chlae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt grb2_human Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 SH2/SH3 adapter GRB2 Protein Ash protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV 83A4B0BA1B248DC4 pld2-grb2-1 4 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey physical association physical association Moreover, Grb2 association with PLD2 appeared to be independent of EGFR stimulation of COS7shGrb2 cells 5 pld2-grb2-2 1 6 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component physical association physical association 8 All these imply that PLD2 follows Grb2 localization to specific compartments, but not to the plasma membrane upon stimulation by EGF. PLD2 colocalizes with Grb2 in the perinuclear region, and this colocalization becomes more pronounced upon stimulation by EGF EGF pld2-grb2-3 2 6 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined tyr169 phe178 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role neutral component neutral component P62993:p.Arg86Lys mutation mutation certain certain sequence position certain certain certain sequence position certain R K Wed May 02 18:19:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg86lys' Tue Jun 30 20:26:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg86Lys' physical association physical association 6, 7 PLD2 colocalizes with Grb2 in the perinuclear Golgi region of COS7shGrb2 cells. EGF pld2-sos 4 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association PLD2 WT interacts with Grb2/Sos 4a pld2-grb2-4 3 6 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait physical association physical association PLD2 WT interacts with Grb2/Sos 4a