MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Reconstitution and subunit geometry of human condensin complexes. Reconstitution and subunit geometry of human condensin complexes. EMBO J. (0261-4189) 2007 imex curation Onn I., Aono N., Hirano M., Hirano T. hirano@cshl.edu 2009-01-08 Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip autoradiography autoradiography Onn I., Aono N., Hirano M., Hirano T. hirano@cshl.edu EMBO J. (0261-4189) 2007 2009-01-08 Only protein-protein interactions imex curation Reconstitution and subunit geometry of human condensin complexes. Reconstitution and subunit geometry of human condensin complexes. EMBO J. (0261-4189) 2007 imex curation Onn I., Aono N., Hirano M., Hirano T. hirano@cshl.edu 2009-01-08 Only protein-protein interactions spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Onn I., Aono N., Hirano M., Hirano T. hirano@cshl.edu EMBO J. (0261-4189) 2007 2009-01-08 Only protein-protein interactions imex curation Reconstitution and subunit geometry of human condensin complexes. Reconstitution and subunit geometry of human condensin complexes. EMBO J. (0261-4189) 2007 imex curation Onn I., Aono N., Hirano M., Hirano T. hirano@cshl.edu 2009-01-08 Only protein-protein interactions spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection pull down pull down western blot western blot Immuno blot Immuno blot Onn I., Aono N., Hirano M., Hirano T. hirano@cshl.edu EMBO J. (0261-4189) 2007 2009-01-08 Only protein-protein interactions imex curation cndh2_human Condensin-2 complex subunit H2 NCAPH2 CAPH2 Non-SMC condensin II complex subunit H2 Chromosome-associated protein H2 Kleisin-beta protein protein human Homo sapiens Human MEDVEARFAHLLQPIRDLTKNWEVDVAAQLGEYLEELDQICISFDEGKTTMNFIEAALLIQGSACVYSKKVEYLYSLVYQALDFISGKRRAKQLSSVQEDRANGVASSGVPQEAENEFLSLDDFPDSRTNVDLKNDQTPSEVLIIPLLPMALVAPDEMEKNNNPLYSRQGEVLASRKDFRMNTCVPHPRGAFMLEPEGMSPMEPAGVSPMPGTQKDTGRTEEQPMEVSVCRSPVPALGFSQEPGPSPEGPMPLGGGEDEDAEEAVELPEASAPKAALEPKESRSPQQSAALPRRYMLREREGAPEPASCVKETPDPWQSLDPFDSLESKPFKKGRPYSVPPCVEEALGQKRKRKGAAKLQDFHQWYLAAYADHADSRRLRRKGPSFADMEVLYWTHVKEQLETLRKLQRREVAEQWLRPAEEDHLEDSLEDLGAADDFLEPEEYMEPEGADPREAADLDAVPMSLSYEELVRRNVELFIATSQKFVQETELSQRIRDWEDTVQPLLQEQEQHVPFDIHTYGDQLVSRFPQLNEWCPFAELVAGQPAFEVCRSMLASLQLANDYTVEITQQPGLEMAVDTMSLRLLTHQRAHKRFQTYAAPSMAQP DIP protein Q6IBW4 original sequence version: 51 2C8A1070C27B4547 cndd3_human Condensin-2 complex subunit D3 Non-SMC condensin II complex subunit D3 NCAPD3 CAPD3 KIAA0056 protein protein human Homo sapiens Human MVALRGLGSGLQPWCPLDLRLEWVDTVWELDFTETEPLDPSIEAEIIETGLAAFTKLYESLLPFATGEHGSMESIWTFFIENNVSHSTLVALFYHFVQIVHKKNVSVQYREYGLHAAGLYFLLLEVPGSVANQVFHPVMFDKCIQTLKKSWPQESNLNRKRKKEQPKSSQANPGRHRKRGKPPRREDIEMDEIIEEQEDENICFSARDLSQIRNAIFHLLKNFLRLLPKFSLKEKPQCVQNCIEVFVSLTNFEPVLHECHVTQARALNQAKYIPELAYYGLYLLCSPIHGEGDKVISCVFHQMLSVILMLEVGEGSHRAPLAVTSQVINCRNQAVQFISALVDELKESIFPVVRILLQHICAKVVDKSEYRTFAAQSLVQLLSKLPCGEYAMFIAWLYKYSRSSKIPHRVFTLDVVLALLELPEREVDNTLSLEHQKFLKHKFLVQEIMFDRCLDKAPTVRSKALSSFAHCLELTVTSASESILELLINSPTFSVIESHPGTLLRNSSAFSYQRQTSNRSEPSGEINIDSSGETVGSGERCVMAMLRRRIRDEKTNVRKSALQVLVSILKHCDVSGMKEDLWILQDQCRDPAVSVRKQALQSLTELLMAQPRCVQIQKAWLRGVVPVVMDCESTVQEKALEFLDQLLLQNIRHHSHFHSGDDSQVLAWALLTLLTTESQELSRYLNKAFHIWSKKEKFSPTFINNVISHTGTEHSAPAWMLLSKIAGSSPRLDYSRIIQSWEKISSQQNPNSNTLGHILCVIGHIAKHLPKSTRDKVTDAVKCKLNGFQWSLEVISSAVDALQRLCRASAETPAEEQELLTQVCGDVLSTCEHRLSNIVLKENGTGNMDEDLLVKYIFTLGDIAQLCPARVEKRIFLLIQSVLASSADADHSPSSQGSSEAPASQPPPQVRGSVMPSVIRAHAIITLGKLCLQHEDLAKKSIPALVRELEVCEDVAVRNNVIIVMCDLCIRYTIMVDKYIPNISMCLKDSDPFIRKQTLILLTNLLQEEFVKWKGSLFFRFVSTLIDSHPDIASFGEFCLAHLLLKRNPVMFFQHFIECIFHFNNYEKHEKYNKFPQSEREKRLFSLKGKSNKERRMKIYKFLLEHFTDEQRFNITSKICLSILACFADGILPLDLDASELLSDTFEVLSSKEIKLLAMRSKPDKDLLMEEDDMALANVVMQEAQKKLISQVQKRNFIENIIPIIISLKTVLEKNKIPALRELMHYLREVMQDYRDELKDFFAVDKQLASELEYDMKKYQEQLVQEQELAKHADVAGTAGGAEVAPVAQVALCLETVPVPAGQENPAMSPAVSQPCTPRASAGHVAVSSPTPETGPLQRLLPKARPMSLSTIAILNSVKKAVESKSRHRSRSLGVLPFTLNSGSPEKTCSQVSSYSLEQESNGEIEHVTKRAISTPEKSISDVTFGAGVSYIGTPRTPSSAKEKIEGRSQGNDILCLSLPDKPPPQPQQWNVRSPARNKDTPACSRRSLRKTPLKTAN DIP protein P42695 original sequence version: 76 038033DA393E4310 cnd1_human Condensin complex subunit 1 NCAPD2 CAPD2 KIAA0159 Non-SMC condensin I complex subunit D2 Chromosome condensation-related SMC-associated protein 1 Chromosome-associated protein D2 XCAP-D2 homolog CNAP1 protein protein human Homo sapiens Human MAPQMYEFHLPLSPEELLKSGGVNQYVVQEVLSIKHLPPQLRAFQAAFRAQGPLAMLQHFDTIYSILHHFRSIDPGLKEDTLQFLIKVVSRHSQELPAILDDTTLSGSDRNAHLNALKMNCYALIRLLESFETMASQTNLVDLDLGGKGKKARTKAAHGFDWEEERQPILQLLTQLLQLDIRHLWNHSIIEEEFVSLVTGCCYRLLENPTINHQKNRPTREAITHLLGVALTRYNHMLSATVKIIQMLQHFEHLAPVLVAAVSLWATDYGMKSIVGEIVREIGQKCPQELSRDPSGTKGFAAFLTELAERVPAILMSSMCILLDHLDGENYMMRNAVLAAMAEMVLQVLSGDQLEAAARDTRDQFLDTLQAHGHDVNSFVRSRVLQLFTRIVQQKALPLTRFQAVVALAVGRLADKSVLVCKNAIQLLASFLANNPFSCKLSDADLAGPLQKETQKLQEMRAQRRTAAASAVLDPEEEWEAMLPELKSTLQQLLQLPQGEEEIPEQIANTETTEDVKGRIYQLLAKASYKKAIILTREATGHFQESEPFSHIDPEESEETRLLNILGLIFKGPAASTQEKNPRESTGNMVTGQTVCKNKPNMSDPEESRGNDELVKQEMLVQYLQDAYSFSRKITEAIGIISKMMYENTTTVVQEVIEFFVMVFQFGVPQALFGVRRMLPLIWSKEPGVREAVLNAYRQLYLNPKGDSARAKAQALIQNLSLLLVDASVGTIQCLEEILCEFVQKDELKPAVTQLLWERATEKVACCPLERCSSVMLLGMMARGKPEIVGSNLDTLVSIGLDEKFPQDYRLAQQVCHAIANISDRRKPSLGKRHPPFRLPQEHRLFERLRETVTKGFVHPDPLWIPFKEVAVTLIYQLAEGPEVICAQILQGCAKQALEKLEEKRTSQEDPKESPAMLPTFLLMNLLSLAGDVALQQLVHLEQAVSGELCRRRVLREEQEHKTKDPKEKNTSSETTMEEELGLVGATADDTEAELIRGICEMELLDGKQTLAAFVPLLLKVCNNPGLYSNPDLSAAASLALGKFCMISATFCDSQLRLLFTMLEKSPLPIVRSNLMVATGDLAIRFPNLVDPWTPHLYARLRDPAQQVRKTAGLVMTHLILKDMVKVKGQVSEMAVLLIDPEPQIAALAKNFFNELSHKGNAIYNLLPDIISRLSDPELGVEEEPFHTIMKQLLSYITKDKQTESLVEKLCQRFRTSRTERQQRDLAYCVSQLPLTERGLRKMLDNFDCFGDKLSDESIFSAFLSVVGKLRRGAKPEGKAIIDEFEQKLRACHTRGLDGIKELEIGQAGSQRAPSAKKPSTGSRYQPLASTASDNDFVTPEPRRTTRRHPNTQQRASKKKPKVVFSSDESSEEDLSAEMTEDETPKKTTPILRASARRHRS 35E31642B94B513D cnd3_human Condensin complex subunit 3 NCAPG NYMEL3 CAPG Chromosome-associated protein G Condensin subunit CAP-G XCAP-G homolog Melanoma antigen NY-MEL-3 Non-SMC condensin I complex subunit G protein protein human Homo sapiens Human MGAERRLLSIKEAFRLAQQPHQNQAKLVVALSRTYRTMDDKTVFHEEFIHYLKYVMVVYKREPAVERVIEFAAKFVTSFHQSDMEDDEEEEDGGLLNYLFTFLLKSHEANSNAVRFRVCLLINKLLGSMPENAQIDDDVFDKINKAMLIRLKDKIPNVRIQAVLALSRLQDPKDDECPVVNAYATLIENDSNPEVRRAVLSCIAPSAKTLPKIVGRTKDVKEAVRKLAYQVLAEKVHMRAMSIAQRVMLLQQGLNDRSDAVKQAMQKHLLQGWLRFSEGNILELLHRLDVENSSEVAVSVLNALFSITPLSELVGLCKNNDGRKLIPVETLTPEIALYWCALCEYLKSKGDEGEEFLEQILPEPVVYADYLLSYIQSIPVVNEEHRGDFSYIGNLMTKEFIGQQLILIIKSLDTSEEGGRKKLLAVLQEILILPTIPISLVSFLVERLLHIIIDDNKRTQIVTEIISEIRAPIVTVGVNNDPADVRKKELKMAEIKVKLIEAKEALENCITLQDFNRASELKEEIKALEDARINLLKETEQLEIKEVHIEKNDAETLQKCLILCYELLKQMSISTGLSATMNGIIESLILPGIISIHPVVRNLAVLCLGCCGLQNQDFARKHFVLLLQVLQIDDVTIKISALKAIFDQLMTFGIEPFKTKKIKTLHCEGTEINSDDEQESKEVEETATAKNVLKLLSDFLDSEVSELRTGAAEGLAKLMFSGLLVSSRILSRLILLWYNPVTEEDVQLRHCLGVFFPVFAYASRTNQECFEEAFLPTLQTLANAPASSPLAEIDITNVAELLVDLTRPSGLNPQAKTSQDYQALTVHDNLAMKICNEILTSPCSPEIRVYTKALSSLELSSHLAKDLLVLLNEILEQVKDRTCLRALEKIKIQLEKGNKEFGDQAEAAQDATLTTTTFQNEDEKNKEVYMTPLRGVKATQASKSTQLKTNRGQRKVTVSARTNRRCQTAEADSESDHEVPEPESEMKMRLPRRAKTAALEKSKLNLAQFLNEDLS DIP protein Q9BPX3 original sequence version: 129 D9ACC205C48F3AF5 cndg2_human Condensin-2 complex subunit G2 Chromosome-associated protein G2 Non-SMC condensin II complex subunit G2 Leucine zipper protein 5 NCAPG2 LUZP5 protein protein human Homo sapiens Human MEKRETFVQAVSKELVGEFLQFVQLDKEASDPFSLNELLDELSRKQKEELWQRLKNLLTDVLLESPVDGWQVVEAQGEDNMETEHGSKMRKSIEIIYAITSVILASVSVINESENYEALLECVIILNGILYALPESERKLQSSIQDLCVTWWEKGLPAKEDTGKTAFVMLLRRSLETKTGADVCRLWRIHQALYCFDYDLEESGEIKDMLLECFININYIKKEEGRRFLSCLFNWNINFIKMIHGTIKNQLQGLQKSLMVYIAEIYFRAWKKASGKILEAIENDCIQDFMFHGIHLPRRSPVHSKVREVLSYFHHQKKVRQGVEEMLYRLYKPILWRGLKARNSEVRSNAALLFVEAFPIRDPNLHAIEMDSEIQKQFEELYSLLEDPYPMVRSTGILGVCKITSKYWEMMPPTILIDLLKKVTGELAFDTSSADVRCSVFKCLPMILDNKLSHPLLEQLLPALRYSLHDNSEKVRVAFVDMLLKIKAVRAAKFWKICPMEHILVRLETDSRPVSRRLVSLIFNSFLPVNQPEEVWCERCVTLVQMNHAAARRFYQYAHEHTACTNIAKLIHVIRHCLNACIQRAVREPPEDEEEEDGREKENVTVLDKTLSVNDVACMAGLLEIIVILWKSIDRSMENNKEAKLYTINKFASVLPEYLKVFKDDRCKIPLFMLMSFMPASAVPPFSCGVISTLRSREEGAVDKSYCTLLDCLCSWGQVGHILELVDNWLPTEHAQAKSNTASKGRVQIHDTRPVKPELALVYIEYLLTHPKNRECLLSAPRKKLNHLLKALETSKADLESLLQTPGGKPRGFSEAAAPRAFGLHCRLSIHLQHKFCSEGKVYLSMLEDTGFWLESKILSFIQDQEEDYLKLHRVIYQQIIQTYLTVCKDVVMVGLGDHQFQMQLLQRSLGIMQTVKGFFYVSLLLDILKEITGSSLIQKTDSDEEVAMLLDTVQKVFQKMLECIARSFRKQPEEGLRLLYSVQRPLHEFITAVQSRHTDTPVHRGVLSTLIAGPVVEISHQLRKVSDVEELTPPEHLSDLPPFSRCLIGIIIKSSNVVRSFLDELKACVASNDIEGIVCLTAAVHIILVINAGKHKSSKVREVAATVHRKLKTFMEITLEEDSIERFLYESSSRTLGELLNS DIP protein Q86XI2 original sequence version: 54 7C7B7FA2A7F0683C cnd2_human Condensin complex subunit 2 Non-SMC condensin I complex subunit H Barren homolog protein 1 Chromosome-associated protein H XCAP-H homolog NCAPH BRRN CAPH KIAA0074 BRRN1 protein protein human Homo sapiens Human MGPPGPALPATMNNSSSETRGHPHSASSPSERVFPMPLPRKAPLNIPGTPVLEDFPQNDDEKERLQRRRSRVFDLQFSTDSPRLLASPSSRSIDISATIPKFTNTQITEHYSTCIKLSTENKITTKNAFGLHLIDFMSEILKQKDTEPTNFKVAAGTLDASTKIYAVRVDAVHADVYRVLGGLGKDAPSLEEVEGHVADGSATEMGTTKKAVKPKKKHLHRTIEQNINNLNVSEADRKCEIDPMFQKTAASFDECSTAGVFLSTLHCQDYRSELLFPSDVQTLSTGEPLELPELGCVEMTDLKAPLQQCAEDRQICPSLAGFQFTQWDSETHNESVSALVDKFKKNDQVFDINAEVDESDCGDFPDGSLGDDFDANDEPDHTAVGDHEEFRSWKEPCQVQSCQEEMISLGDGDIRTMCPLLSMKPGEYSYFSPRTMSMWAGPDHWRFRPRRKQDAPSQSENKKKSTKKDFEIDFEDDIDFDVYFRKTKAATILTKSTLENQNWRATTLPTDFNYNVDTLVQLHLKPGTRLLKMAQGHRVETEHYEEIEDYDYNNPNDTSNFCPGLQAADSDDEDLDDLFVGPVGNSDLSPYPCHPPKTAQQNGDTPEAQGLDITTYGESNLVAEPQKVNKIEIHYAKTAKKMDMKKLKQSMWSLLTALSGKEADAEANHREAGKEAALAEVADEKMLSGLTKDLQRSLPPVMAQNLSIPLAFACLLHLANEKNLKLEGTEDLSDVLVRQGD DIP protein Q15003 original sequence version: 97 B5B34A36CAE0C28E smc2_human Structural maintenance of chromosomes protein 2 PRO0324 SMC2 CAPE SMC2L1 Chromosome-associated protein E XCAP-E homolog protein protein human Homo sapiens Human MHIKSIILEGFKSYAQRTEVNGFDPLFNAITGLNGSGKSNILDSICFLLGISNLSQVRASNLQDLVYKNGQAGITKASVSITFDNSDKKQSPLGFEVHDEITVTRQVVIGGRNKYLINGVNANNTRVQDLFCSVGLNVNNPHFLIMQGRITKVLNMKPPEILSMIEEAAGTRMYEYKKIAAQKTIEKKEAKLKEIKTILEEEITPTIQKLKEERSSYLEYQKVMREIEHLSRLYIAYQFLLAEDTKVRSAEELKEMQDKVIKLQEELSENDKKIKALNHEIEELEKRKDKETGGILRSLEDALAEAQRVNTKSQSAFDLKKKNLACEESKRKELEKNMVEDSKTLAAKEKEVKKITDGLHALQEASNKDAEALAAAQQHFNAVSAGLSSNEDGAEATLAGQMMACKNDISKAQTEAKQAQMKLKHAQQELKNKQAEVKKMDSGYRKDQEALEAVKRLKEKLEAEMKKLNYEENKEESLLEKRRQLSRDIGRLKETYEALLARFPNLRFAYKDPEKNWNRNCVKGLVASLISVKDTSATTALELVAGERLYNVVVDTEVTGKKLLERGELKRRYTIIPLNKISARCIAPETLRVAQNLVGPDNVHVALSLVEYKPELQKAMEFVFGTTFVCDNMDNAKKVAFDKRIMTRTVTLGGDVFDPHGTLSGGARSQAASILTKFQELKDVQDELRIKENELRALEEELAGLKNTAEKYRQLKQQWEMKTEEADLLQTKLQQSSYHKQQEELDALKKTIEESEETLKNTKEIQRKAEEKYEVLENKMKNAEAERERELKDAQKKLDCAKTKADASSKKMKEKQQEVEAITLELEELKREHTSYKQQLEAVNEAIKSYESQIEVMAAEVAKNKESVNKAQEEVTKQKEVITAQDTVIKAKYAEVAKHKEQNNDSQLKIKELDHNISKHKREAEDGAAKVSKMLKDYDWINAERHLFGQPNSAYDFKTNNPKEAGQRLQKLQEMKEKLGRNVNMRAMNVLTEAEERYNDLMKKKRIVENDKSKILTTIEDLDQKKNQALNIAWQKVNKDFGSIFSTLLPGANAMLAPPEGQTVLDGLEFKVALGNTWKENLTELSGGQRSLVALSLILSMLLFKPAPIYILDEVDAALDLSHTQNIGQMLRTHFTHSQFIVVSLKEGMFNNANVLFKTKFVDGVSTVARFTQCQNGKISKEAKSKAKPPKGAHVEV DIP protein O95347 original sequence version: 87 DIP protein O95347 original sequence version: 95 DIP protein O95347 original sequence version: 139 15EBB56B31FC1364 smc4_human Structural maintenance of chromosomes protein 4 SMC4 CAPC SMC4L1 Chromosome-associated polypeptide C XCAP-C homolog protein protein human Homo sapiens Human MPRKGTQPSTARRREEGPPPPSPDGASSDAEPEPPSGRTESPATAAETASEELDNRSLEEILNSIPPPPPPAMTNEAGAPRLMITHIVNQNFKSYAGEKILGPFHKRFSCIIGPNGSGKSNVIDSMLFVFGYRAQKIRSKKLSVLIHNSDEHKDIQSCTVEVHFQKIIDKEGDDYEVIPNSNFYVSRTACRDNTSVYHISGKKKTFKDVGNLLRSHGIDLDHNRFLILQGEVEQIAMMKPKGQTEHDEGMLEYLEDIIGCGRLNEPIKVLCRRVEILNEHRGEKLNRVKMVEKEKDALEGEKNIAIEFLTLENEIFRKKNHVCQYYIYELQKRIAEMETQKEKIHEDTKEINEKSNILSNEMKAKNKDVKDTEKKLNKITKFIEENKEKFTQLDLEDVQVREKLKHATSKAKKLEKQLQKDKEKVEEFKSIPAKSNNIINETTTRNNALEKEKEKEEKKLKEVMDSLKQETQGLQKEKESREKELMGFSKSVNEARSKMDVAQSELDIYLSRHNTAVSQLTKAKEALIAASETLKERKAAIRDIEGKLPQTEQELKEKEKELQKLTQEETNFKSLVHDLFQKVEEAKSSLAMNRSRGKVLDAIIQEKKSGRIPGIYGRLGDLGAIDEKYDVAISSCCHALDYIVVDSIDIAQECVNFLKRQNIGVATFIGLDKMAVWAKKMTEIQTPENTPRLFDLVKVKDEKIRQAFYFALRDTLVADNLDQATRVAYQKDRRWRVVTLQGQIIEQSGTMTGGGSKVMKGRMGSSLVIEISEEEVNKMESQLQNDSKKAMQIQEQKVQLEERVVKLRHSEREMRNTLEKFTASIQRLIEQEEYLNVQVKELEANVLATAPDKKKQKLLEENVSAFKTEYDAVAEKAGKVEAEVKRLHNTIVEINNHKLKAQQDKLDKINKQLDECASAITKAQVAIKTADRNLQKAQDSVLRTEKEIKDTEKEVDDLTAELKSLEDKAAEVVKNTNAAEESLPEIQKEHRNLLQELKVIQENEHALQKDALSIKLKLEQIDGHIAEHNSKIKYWHKEISKISLHPIEDNPIEEISVLSPEDLEAIKNPDSITNQIALLEARCHEMKPNLGAIAEYKKKEELYLQRVAELDKITYERDSFRQAYEDLRKQRLNEFMAGFYIITNKLKENYQMLTLGGDAELELVDSLDPFSEGIMFSVRPPKKSWKKIFNLSGGEKTLSSLALVFALHHYKPTPLYFMDEIDAALDFKNVSIVAFYIYEQTKNAQFIIISLRNNMFEISDRLIGIYKTYNITKSVAVNPKEIASKGLC DIP protein Q9NTJ3 original sequence version: 91 9D164D6EB0602464 smc2-smc4-8 2 SMC4 11 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell SMC2 10 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell physical association physical association F3A EMBO-Journal homomint mint smc2-ncaph2-1 2 SMC2 10 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell CAP-H2 4 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell physical association physical association F3C EMBO-Journal homomint mint smc2-ncaph-2 2 SMC2 10 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell CAP-H 9 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell physical association physical association F3C EMBO-Journal homomint mint smc4-ncaph-1 2 SMC4 11 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell CAP-H 9 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell physical association physical association F3C EMBO-Journal homomint mint smc4-ncaph2-1 2 CAP-H2 4 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell SMC4 11 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell physical association physical association F3C EMBO-Journal homomint mint ncaph2-ncapg2-1 2 CAP-G2 8 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell CAP-H2 4 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell physical association physical association F3B EMBO-Journal homomint mint ncaph2-ncapd3-1 2 CAP-H2 4 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell CAP-D3 5 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell physical association physical association F3B EMBO-Journal homomint mint ncapd2-ncapg-1 2 CAP-D2 6 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell CAP-G 7 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell physical association physical association F3B EMBO-Journal homomint mint ncaph-ncapg-2 2 CAP-G 7 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell CAP-H 9 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell physical association physical association F3B EMBO-Journal homomint mint ncaph-ncapd2-2 2 CAP-H 9 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell CAP-D2 6 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell physical association physical association F3B EMBO-Journal homomint mint ncaph-ncapd2-1 1 CAPD2 6 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence autoradiography autoradiography unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated CAPH 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence autoradiography autoradiography unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated physical association physical association F4A F5A F5A F4B F4D EMBO-Journal homomint mint ncaph-ncapg-1 1 CAP-G 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence autoradiography autoradiography unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated CAPH 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence autoradiography autoradiography unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated physical association physical association H(395-730) does not bind D2 (F4B to F4D) F4B F4D F5B F4B F4D EMBO-Journal homomint mint ncapd2-ncaph-1 1 CAPH 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence autoradiography autoradiography unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain range MINT-4330196: original sequence: 1-393 : MGPPGPALPATMNNSSSETRGHPHSASSPSERVFPMPLPRKAPLNIPGTPVLEDFPQNDDEKERLQRRRSRVFDLQFSTDSPRLLASPSSRSIDISATIPKFTNTQITEHYSTCIKLSTENKITTKNAFGLHLIDFMSEILKQKDTEPTNFKVAAGTLDASTKIYAVRVDAVHADVYRVLGGLGKDAPSLEEVEGHVADGSATEMGTTKKAVKPKKKHLHRTIEQNINNLNVSEADRKCEIDPMFQKTAASFDECSTAGVFLSTLHCQDYRSELLFPSDVQTLSTGEPLELPELGCVEMTDLKAPLQQCAEDRQICPSLAGFQFTQWDSETHNESVSALVDKFKKNDQVFDINAEVDESDCGDFPDGSLGDDFDANDEPDHTAVGDHEEFRS CAPD2 6 western blot western blot Immuno blot Immuno blot autoradiography autoradiography unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated physical association physical association H(1-393) does not bind G, cf to F4A/F4B. F4C EMBO-Journal homomint mint ncaph-smc4-1 3 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f3d To further narrow down interaction domains, we constructed recombinant viruses that express the N-terminal (amino acids 1–393) or C-terminal half (amino acids 395–730) of hCAP-H. It was found that the N-terminal half of hCAP-H binds to hCAP-D2 and hSMC2, but not to hCAP-G or hSMC4 (Figure 3D, lanes 1 and 2). Conversely, the C-terminal half of hCAP-H interacted with hCAP-G and hSMC4, but not with hCAP-D2 or hSMC2 (Figure 3D, lanes 3 and 4). ncaph-smc4-2 3 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association f3d To further narrow down interaction domains, we constructed recombinant viruses that express the N-terminal (amino acids 1–393) or C-terminal half (amino acids 395–730) of hCAP-H. It was found that the N-terminal half of hCAP-H binds to hCAP-D2 and hSMC2, but not to hCAP-G or hSMC4 (Figure 3D, lanes 1 and 2). Conversely, the C-terminal half of hCAP-H interacted with hCAP-G and hSMC4, but not with hCAP-D2 or hSMC2 (Figure 3D, lanes 3 and 4). ncaph-ncapg-3 3 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f3d' To further narrow down interaction domains, we constructed recombinant viruses that express the N-terminal (amino acids 1–393) or C-terminal half (amino acids 395–730) of hCAP-H. It was found that the N-terminal half of hCAP-H binds to hCAP-D2 and hSMC2, but not to hCAP-G or hSMC4 (Figure 3D, lanes 1 and 2). Conversely, the C-terminal half of hCAP-H interacted with hCAP-G and hSMC4, but not with hCAP-D2 or hSMC2 (Figure 3D, lanes 3 and 4). ncaph2-ncapd3-2 3 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f3d' To further narrow down interaction domains, we constructed recombinant viruses that express the N-terminal (amino acids 1–393) or C-terminal half (amino acids 395–730) of hCAP-H. It was found that the N-terminal half of hCAP-H binds to hCAP-D2 and hSMC2, but not to hCAP-G or hSMC4 (Figure 3D, lanes 1 and 2). Conversely, the C-terminal half of hCAP-H interacted with hCAP-G and hSMC4, but not with hCAP-D2 or hSMC2 (Figure 3D, lanes 3 and 4). ncaph-ncapd2-3 3 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association f4d These observations were further confirmed by reciprocal reactions that pulled down the N-terminal or the C-terminal domain of hCAP-H (Figure 4D). H(1-393) does not bind G, cf to F4B ncaph-ncapd2-4 1 7 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated CAPD2 6 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence autoradiography autoradiography unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated CAPH 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence autoradiography autoradiography unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated physical association physical association F4A F4B F5A F4B F4D EMBO-Journal homomint mint ncaph-ncapg-4 1 CAP-G 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence autoradiography autoradiography unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated CAPH 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence autoradiography autoradiography unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated physical association physical association H(395-730) does not bind D2 F4C F5B F4B F4D EMBO-Journal homomint mint ncaph-ncapg-5 1 CAP-G 7 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence autoradiography autoradiography unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain CAPH 9 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence autoradiography autoradiography unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated physical association physical association H(395-730) does not bind D2 (F4B to F4D) F5B F5B F4B F4D EMBO-Journal homomint mint