IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cell (0092-8674) 2007 imex curation Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis ludger.hengst@i-med.ac.at 12-MAR-2007: Contacted by JYOTI. Only protein-protein interactions 12 human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis ludger.hengst@i-med.ac.at Accepted 2007-MAR-1 by JYOTI. 12-MAR-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 12 imex curation Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cell (0092-8674) 2007 imex curation Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis ludger.hengst@i-med.ac.at 12-MAR-2007: Contacted by JYOTI. Only protein-protein interactions 12 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis Identity of Cyclin A taken from reference Lacy et al. 2004 (PMID:15024385). ludger.hengst@i-med.ac.at Accepted 2007-MAR-1 by JYOTI. 12-MAR-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 12 imex curation Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cell (0092-8674) 2007 imex curation Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis ludger.hengst@i-med.ac.at 12-MAR-2007: Contacted by JYOTI. Only protein-protein interactions 12 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis ludger.hengst@i-med.ac.at Accepted 2007-MAR-1 by JYOTI. 12-MAR-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 12 imex curation Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cell (0092-8674) 2007 imex curation Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis ludger.hengst@i-med.ac.at 12-MAR-2007: Contacted by JYOTI. Only protein-protein interactions 12 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis Identity of Cyclin A taken from reference Lacy et al. 2004 (PMID:15024385). ludger.hengst@i-med.ac.at Accepted 2007-MAR-1 by JYOTI. 12-MAR-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 12 imex curation Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cell (0092-8674) 2007 imex curation Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis ludger.hengst@i-med.ac.at 12-MAR-2007: Contacted by JYOTI. Only protein-protein interactions 12 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis ludger.hengst@i-med.ac.at Accepted 2007-MAR-1 by JYOTI. 12-MAR-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 12 imex curation Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases. Cell (0092-8674) 2007 imex curation Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis ludger.hengst@i-med.ac.at 12-MAR-2007: Contacted by JYOTI. Only protein-protein interactions 12 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Grimmler M., Wang Y., Mund T., Cilensek Z., Keidel EM., Waddell MB., Jakel H., Kullmann M., Kriwacki RW., Hengst L. Apoptosis - Interactions involving proteins with a function related to apoptosis Further detail on Lyn kinase taken from manufacturer's website. Identity of Cyclin A taken from reference Lacy et al. 2004 (PMID:15024385). ludger.hengst@i-med.ac.at Accepted 2007-MAR-1 by JYOTI. 12-MAR-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 12 imex curation lyn_human Tyrosine-protein kinase Lyn JTK8 Lck/Yes-related novel protein tyrosine kinase V-yes-1 Yamaguchi sarcoma viral related oncogene homolog p53Lyn p56Lyn LYN protein protein human Homo sapiens Human MGCIKSKGKDSLSDDGVDLKTQPVRNTERTIYVRDPTSNKQQRPVPESQLLPGQRFQTKDPEEQGDIVVALYPYDGIHPDDLSFKKGEKMKVLEEHGEWWKAKSLLTKKEGFIPSNYVAKLNTLETEEWFFKDITRKDAERQLLAPGNSAGAFLIRESETLKGSFSLSVRDFDPVHGDVIKHYKIRSLDNGGYYISPRITFPCISDMIKHYQKQADGLCRRLEKACISPKPQKPWDKDAWEIPRESIKLVKRLGAGQFGEVWMGYYNNSTKVAVKTLKPGTMSVQAFLEEANLMKTLQHDKLVRLYAVVTREEPIYIITEYMAKGSLLDFLKSDEGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSESLMCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGCFTIKSDVWSFGILLYEIVTYGKIPYPGRTNADVMTALSQGYRMPRVENCPDELYDIMKMCWKEKAEERPTFDYLQSVLDDFYTATEGQYQQQP DIP protein P07948 original sequence version: 141 408D3D461204E378 ccnd1_human G1/S-specific cyclin-D1 CCND1 BCL1 PRAD1 PRAD1 oncogene BCL-1 oncogene B-cell lymphoma 1 protein protein protein human Homo sapiens Human MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI DIP protein P24385 original sequence version: 163 DIP protein P24385 original sequence version: 165 DIP protein P24385 original sequence version: 160 DIP protein P24385 original sequence version: 121 DIP protein P24385 original sequence version: 122 DIP protein P24385 original sequence version: 143 DIP protein P24385 original sequence version: 127 3CC00C9905F58D3A cdn1b_human Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 CDKN1B KIP1 protein protein human Homo sapiens Human MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT DIP protein P46527 original sequence version: 120 DIP protein P46527 original sequence version: 115 DIP protein P46527 original sequence version: 154 DIP protein P46527 original sequence version: 168 DIP protein P46527 original sequence version: 164 DIP protein P46527 original sequence version: 118 1118D58901CDF3FC abl1_mouse Tyrosine-protein kinase ABL1 Abl1 Abl Abelson murine leukemia viral oncogene homolog 1 Proto-oncogene c-Abl p150 Abelson tyrosine-protein kinase 1 protein protein mouse Mus musculus Mouse MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTIYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVSAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKRGTRGGAGSMLQAPELPTKTRTCRRAAEQKDAPDTPELLHTKGLGESDALDSEPAVSPLLPRKERGPPDGSLNEDERLLPRDRKTNLFSALIKKKKKMAPTPPKRSSSFREMDGQPDRRGASEDDSRELCNGPPALTSDAAEPTKSPKASNGAGVPNGAFREPGNSGFRSPHMWKKSSTLTGSRLAAAEEESGMSSSKRFLRSCSASCMPHGARDTEWRSVTLPRDLPSAGKQFDSSTFGGHKSEKPALPRKRTSESRSEQVAKSTAMPPPRLVKKNEEAAEEGFKDTESSPGSSPPSLTPKLLRRQVTASPSSGLSHKEEATKGSASGMGTPATAEPAPPSNKVGLSKASSEEMRVRRHKHSSESPGRDKGRLAKLKPAPPPPPACTGKAGKPAQSPSQEAGEAGGPTKTKCTSLAMDAVNTDPTKAGPPGEGLRKPVPPSVPKPQSTAKPPGTPTSPVSTPSTAPAPSPLAGDQQPSSAAFIPLISTRVSLRKTRQPPERIASGTITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLESNLRELQICPATASSGPAATQDFSKLLSSVKEISDIVRR BD48ADE8557AE95C cdk2_human Cyclin-dependent kinase 2 CDK2 p33 protein kinase Cell division protein kinase 2 CDKN2 protein protein human Homo sapiens Human MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL DIP protein P24941 original sequence version: 142 DIP protein P24941 original sequence version: 118 DIP protein P24941 original sequence version: 143 DIP protein P24941 original sequence version: 144 DIP protein P24941 original sequence version: 152 DIP protein P24941 original sequence version: 182 DIP protein P24941-1 original sequence version: 204 DIP protein P24941 original sequence version: 90 DIP protein P24941 original sequence version: 190 DIP protein P24941 original sequence version: 168 F90A0F4E70910B51 ccne1_human G1/S-specific cyclin-E1 CCNE1 CCNE protein protein human Homo sapiens Human MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA DIP protein P24864 original sequence version: 114 DIP protein P24864 original sequence version: 150 424DF0B253B7047E ccna2_human Cyclin-A2 Cyclin A CCNA2 CCN1 CCNA protein protein human Homo sapiens Human MLGNSAPGPATREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAALAVLKSGNPRGLAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKIEREDALAFNSAISLPGPRKPLVPLDYPMDGSFESPHTMDMSIILEDEKPVSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNETLHLAVNYIDRFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLKVLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAGAAFHLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKNSKYHGVSLLNPPETLNL DIP protein P20248 original sequence version: 183 DIP protein P20248 original sequence version: 146 DIP protein P20248 original sequence version: 168 DIP protein P20248 original sequence version: 149 DIP protein P20248 original sequence version: 111 97763A2897DD35F4 cdkn1b-lyn-1 3 7 unspecified role unspecified role prey prey P07948:p.Tyr508Pro mutation mutation certain certain sequence position certain certain certain sequence position certain Y P Fri Jul 22 10:59:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr508phe' Wed May 02 20:22:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr508pro' Tue Jun 30 16:49:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr508Pro' 9 unspecified role unspecified role bait bait P46527:p.Pro94_Lys96delinsAlaAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain PPK AAA Wed May 02 15:15:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_pro_lys94-96ala_ala_ala' Tue Jun 30 18:31:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro94_Lys96delinsAlaAlaAla' P46527:p.[Tyr74Ala;Tyr88Ala;Tyr89Ala] mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 19:03:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr74ala,tyr88ala,tyr89ala' Tue Jun 30 20:24:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr74Ala;Tyr88Ala;Tyr89Ala]' physical association physical association Catalytically active Lyn (Tyr508Phe) will only bind mutated CDKN1B (p27). Inactive endogenous Lyn binds endogenous p27. 1B Lyn binds to a p27 mutant protein lacking all three tyrosines. cdkn1b-lyn-2 6 7 enzyme enzyme unspecified role unspecified role n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. 9 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 1D Lyn and Abl phosphorylate Y88 of p27 in vitro. Phosphorylation of CDKN1B (p27) on Tyr-88 (mutational analysis) cdkn1b-abl-1 6 10 enzyme enzyme unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range ecoli Escherichia coli (strain K12) 9 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 1D Lyn and Abl phosphorylate Y88 of p27 in vitro. Phosphorylation of CDKN1B (p27) on Tyr-88 (mutational analysis) cdkn1b-abl-2 6 9 enzyme target enzyme target substrate substrate unspecified role unspecified role ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role ancillary ancillary 11 unspecified role unspecified role ancillary ancillary spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 10 enzyme enzyme unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation 1G While monomeric p27 was a better substrate for Abl phosphorylation, tyrosine phosphorylation of cyclin A/Cdk2-bound p27 was clearly detected (Figure 1G) Phosphorylation of CDKN1B (p27) Binding of CDKN1B (p27) to Cyclin A/CDK2 partially inhibited phosphorylation by cAbl cdkn1b-cdk2-1 4 13 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 11 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 9 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association 2A The results of SEC showed that both unmodified p27 and Y88-phosphorylated p27 (pY88-p27) bound to recombinant cyclin A/Cdk2 (Figure 2A). Both phosphorylated and dephosphorylated p27 bind to Cyclin A2/CDK2 cdkn1b-cdk2-2 2 9 unspecified role unspecified role bait bait P46527:p.Tyr89Phe mutation mutation certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 14:57:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr89phe' Tue Jun 30 16:25:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr89Phe' ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 11 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Table 1 Surface Plasmon Resonance (SPR) Analysis of p27 Species Binding to Cyclin A, Cdk2, and Cyclin A/Cdk2 Tyr-88 of CDKN1B (p27) tyrosine phosphorylated, KD=3.8E-9M. Tyr-88 and Tyr 89 of CDKN1B (p27) mutated to Phe KD=5.8E-9M. Truncated CDKN1B (p27) 22-104, KD=2.9E-9M. Tyr-88 of truncated CDKN1B (p27) 22-104, KD=2.7E-9M. cdkn1b-cdk2-3 2 9 unspecified role unspecified role bait bait P46527:p.Tyr89Phe mutation mutation certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 15:59:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr89phe' Tue Jun 30 20:13:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr89Phe' ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Table 1 Surface Plasmon Resonance (SPR) Analysis of p27 Species Binding to Cyclin A, Cdk2, and Cyclin A/Cdk2 Tyr-88 of CDKN1B (p27) tyrosine phosphorylated, KD=4.5E-6M. Truncated 22-104 CDKN1B (p27)KD=1.9E-7M Tyr-88 of truncated 22-104 CDKN1B (p27)tyrosine phosphorylated, KD=9.0E-7M. cdkn1b-ccna1-1 2 9 unspecified role unspecified role bait bait P46527:p.Tyr89Phe mutation mutation certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 18:41:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr89phe' Tue Jun 30 19:27:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr89Phe' ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Table 1 Surface Plasmon Resonance (SPR) Analysis of p27 Species Binding to Cyclin A, Cdk2, and Cyclin A/Cdk2 Tyr-88 of CDKN1B (p27) tyrosine phosphorylated, KD=4.0E-8M. Tyr-88 of truncated 22-104 CDKN1B (p27)tyrosine phosphorylated, KD=3.03E-8M. Truncated 22-104 CDKN1B (p27) KD=3.87E-8M. ccne1-cdkn1b-1 1 9 unspecified role unspecified role prey prey try-88 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait physical association physical association pY88-p27 binds cyclin D and cyclin E complexes in vivo. 2B ccnd1-cdkn1b-2 1 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey tyr-88 (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain physical association physical association pY88-p27 binds cyclin D and cyclin E complexes in vivo. 2B cdkn1b-cdk2-4 5 13 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 9 unspecified role unspecified role neutral component neutral component P46527:p.Tyr89Phe mutation mutation certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 16:10:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr89phe' Tue Jun 30 18:31:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr89Phe' kid region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Figures 3B, S8C, and S8D We used solution NMR spectroscopy to elucidate the structural consequences of pY88 phosphorylation. cdkn1b-cdk2-5 3 9 enzyme target enzyme target substrate substrate unspecified role unspecified role P46527:p.Tyr89Phe mutation mutation certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 16:17:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr89phe' Tue Jun 30 17:51:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr89Phe' region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role ancillary ancillary spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 11 enzyme enzyme unspecified role unspecified role spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation Fig. 4 Y88-phosphorylated p27 is a superior substrate for Cdk2. Prior phosphorylation of Tyr-88 of CDKN1B increases the rate of phosphorylation of the molecule by CDK2